Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,198,441 T→G A547A (GCA→GCC)  PA14_RS14615 ← acyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,198,4410TG100.0% 1651.2 / NA 425A547A (GCA→GCC) PA14_RS14615acyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (242/183);  total (242/183)

Only 100 of 428 total aligned reads displayed.

TCAGGGTGAAACGGTTTCGGCGCGCCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCTGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGCACCAGCGGACCAG  >  NC_008463/3198294‑3198587
                                                                                                                                                   |                                                                                                                                                  
 cAGGGTGAAACGGTTTCGGCGCGCCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCg                                                                                                                                                 >  2:7098575/1‑150 (MQ=255)
 cAGGGTGAAACGGTTTCGGCGCGCCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCg                                                                                                                                                 >  2:639505/1‑150 (MQ=255)
 cAGGGTGAAACGGTTTCGGCGCGCCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCg                                                                                                                                                 <  1:3879613/150‑1 (MQ=255)
 cAGGGTGAAACGGTTTCGGCGCGCCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCACGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCg                                                                                                                                                 <  2:2875525/150‑1 (MQ=255)
  aGGGTGAAACGGTTTCGGCGCGCCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGt                                                                                                                                                <  2:2519388/150‑1 (MQ=255)
  aGGGTGAAACGGTTTCGGCGCGCCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGt                                                                                                                                                >  2:6843118/1‑150 (MQ=255)
   gggTGAAACGGTTTCGGCGCGCCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTc                                                                                                                                               >  2:651893/1‑150 (MQ=255)
       gAAACGGTTTCGGCGCGCCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCg                                                                                                                                           <  1:3496615/150‑1 (MQ=255)
        aaaCGGTTTCGGCGCGCCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCgg                                                                                                                                          >  1:4865971/1‑150 (MQ=255)
         aaCGGTTTCGGCGCGCCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCCTCAGCGGCCGGCGTCGCCGGc                                                                                                                                         >  2:8791272/1‑150 (MQ=255)
          aCGGTTTCGGCGCGCCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGcc                                                                                                                                        <  1:5123739/150‑1 (MQ=255)
             gTTTCGGCGCGCCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCACCCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGcctcc                                                                                                                                     >  1:342925/1‑150 (MQ=255)
                   gcgcgcCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGcctcctcctcc                                                                                                                               >  1:3000932/1‑150 (MQ=255)
                       gcCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGcc                                                                                                                           >  1:5038949/1‑150 (MQ=255)
                             aGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGccc                                                                                                                     >  2:9674849/1‑150 (MQ=255)
                             aGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGccc                                                                                                                     >  1:5846842/1‑150 (MQ=255)
                             aGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGccc                                                                                                                     >  1:9645988/1‑150 (MQ=255)
                              gTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCa                                                                                                                    >  1:7893615/1‑150 (MQ=255)
                                  cTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTcg                                                                                                                <  2:1207426/150‑1 (MQ=255)
                                   tGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTcgc                                                                                                               <  1:3639325/150‑1 (MQ=255)
                                   tGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTcgc                                                                                                               >  1:9036528/1‑150 (MQ=255)
                                     gAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCgcgc                                                                                                             >  2:1576337/1‑150 (MQ=255)
                                      aaCCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCa                                                                                                            >  1:2691284/1‑150 (MQ=255)
                                       aCCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAg                                                                                                           <  2:2984609/150‑1 (MQ=255)
                                        ccGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAgg                                                                                                          >  2:433103/1‑150 (MQ=255)
                                          gggCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGAc                                                                                                        >  1:7299702/1‑150 (MQ=255)
                                          gggCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGAc                                                                                                        >  1:1423007/1‑150 (MQ=255)
                                          gggCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGAc                                                                                                        >  1:1000816/1‑150 (MQ=255)
                                          gggCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGAc                                                                                                        >  1:1811138/1‑150 (MQ=255)
                                            gCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTAGTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGa                                                                                                      >  2:1935148/1‑150 (MQ=255)
                                               cTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACt                                                                                                   >  1:5246580/1‑150 (MQ=255)
                                                     cgGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGa                                                                                             <  1:8912647/150‑1 (MQ=255)
                                                       gATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGAGCGGCAGCGCCATCAGCGGCCGGCGGCGCCGGCCTCCTCCACCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATc                                                                                           >  2:1697795/1‑150 (MQ=255)
                                                       gATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGGTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATc                                                                                           >  2:9529352/1‑150 (MQ=255)
                                                          aCGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTg                                                                                        <  1:10907860/150‑1 (MQ=255)
                                                            ctGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCAGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGcc                                                                                      <  2:10091652/149‑1 (MQ=255)
                                                             tGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCg                                                                                     <  1:1309288/150‑1 (MQ=255)
                                                             tGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCg                                                                                     >  2:3747450/1‑150 (MQ=255)
                                                                 tataGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTgg                                                                                 >  1:7484498/1‑150 (MQ=255)
                                                                  ataGCAGGCGGGACATGGGACACCTCGGTCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGc                                                                                <  2:10876492/150‑1 (MQ=255)
                                                                   taGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCg                                                                               >  2:10523575/1‑150 (MQ=255)
                                                                     gCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGt                                                                             >  1:2032380/1‑150 (MQ=255)
                                                                       aGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCt                                                                           <  1:3953323/150‑1 (MQ=255)
                                                                       aGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCt                                                                           >  2:3853721/1‑150 (MQ=255)
                                                                           tGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTggg                                                                       <  1:8730759/150‑1 (MQ=255)
                                                                           tGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTggg                                                                       >  1:5642583/1‑150 (MQ=255)
                                                                            ggACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGc                                                                      <  2:1832175/150‑1 (MQ=255)
                                                                            ggACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGc                                                                      >  1:1578278/1‑150 (MQ=255)
                                                                             gACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCa                                                                     <  2:7706770/150‑1 (MQ=255)
                                                                                aTGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGtt                                                                  <  2:9251230/150‑1 (MQ=255)
                                                                                aTGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGtt                                                                  >  2:10392657/1‑150 (MQ=255)
                                                                                aTGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGtt                                                                  >  2:5526295/1‑150 (MQ=255)
                                                                                aTGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCGTGGGCAGtt                                                                  >  1:1503577/1‑150 (MQ=255)
                                                                                  gggACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCg                                                                <  2:4584116/150‑1 (MQ=255)
                                                                                   ggACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCgg                                                               >  1:9313380/1‑150 (MQ=255)
                                                                                     acacCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTa                                                             <  2:1313362/150‑1 (MQ=255)
                                                                                      cacCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTaa                                                            <  2:9001903/150‑1 (MQ=255)
                                                                                      cacCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTaa                                                            <  1:7162715/150‑1 (MQ=255)
                                                                                       acCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACGTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAg                                                           >  1:808652/1‑150 (MQ=255)
                                                                                         cTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCg                                                         <  1:9506763/150‑1 (MQ=255)
                                                                                         cTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCg                                                         >  2:8314734/1‑150 (MQ=255)
                                                                                          tCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCTGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGt                                                        >  2:3554450/1‑150 (MQ=255)
                                                                                          tCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCga                                                        >  2:5010792/1‑149 (MQ=255)
                                                                                          tCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGt                                                        >  2:4411378/1‑150 (MQ=255)
                                                                                           cGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTc                                                       <  1:10415418/150‑1 (MQ=255)
                                                                                            gggCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTca                                                      <  1:6815208/150‑1 (MQ=255)
                                                                                              gCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTcaca                                                    >  2:66998/1‑150 (MQ=255)
                                                                                               cAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACAt                                                   >  1:6386466/1‑150 (MQ=255)
                                                                                               cAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCAGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACAt                                                   >  2:5410057/1‑150 (MQ=255)
                                                                                                aaCCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATg                                                  <  1:4683256/150‑1 (MQ=255)
                                                                                                 aCCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGc                                                 >  1:986305/1‑150 (MQ=255)
                                                                                                    tAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCcgc                                              >  1:10151094/1‑150 (MQ=255)
                                                                                                     aGTTATTGTGATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCGGGTTCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTCAGCGTCACATGCcgcg                                             >  2:10707597/1‑150 (MQ=255)
                                                                                                      gTTATTGTTATGGACTGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCgcgg                                            <  1:23619/150‑1 (MQ=255)
                                                                                                      gTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTGCGGTCAGCGTCACATGCCgcgg                                            >  2:10189908/1‑150 (MQ=255)
                                                                                                       ttATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCgcggc                                           <  2:1129384/150‑1 (MQ=255)
                                                                                                          ttGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGcc                                        >  1:4159251/1‑150 (MQ=255)
                                                                                                          ttGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGAGCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGcc                                        >  2:2283624/1‑150 (MQ=255)
                                                                                                            gTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCtt                                      >  1:2532138/1‑150 (MQ=255)
                                                                                                             ttATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTg                                     >  2:7386749/1‑150 (MQ=255)
                                                                                                              tagggAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGa                                    >  2:1077514/4‑150 (MQ=255)
                                                                                                              tATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGa                                    >  1:6020041/1‑150 (MQ=255)
                                                                                                               aTGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTCAGCGTCACATGCCGCGGCGCCTTGaa                                   >  1:8607728/1‑150 (MQ=255)
                                                                                                                 ggAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGt                                 <  2:7887997/150‑1 (MQ=255)
                                                                                                                  gAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTg                                >  1:3142430/1‑150 (MQ=255)
                                                                                                                    aTGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTgcg                              >  1:4444837/1‑150 (MQ=255)
                                                                                                                    aTGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTgcg                              >  1:3220538/1‑150 (MQ=255)
                                                                                                                       tgcgggtgcggCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCa                           >  2:3946621/1‑150 (MQ=255)
                                                                                                                       tgcgggtgcggCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGGTCGGTAAGCCTGACATGCCGCGGCGCCTGGAAGTGAGCCa                           >  2:5190005/1‑150 (MQ=255)
                                                                                                                            gtgcggCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCGTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGgcgc                      <  1:3274642/150‑1 (MQ=255)
                                                                                                                             tgcggCAGCGCCATCAGCTGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCt                     <  2:315988/150‑1 (MQ=255)
                                                                                                                             tgcggCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCt                     >  1:8342787/1‑150 (MQ=255)
                                                                                                                              gcggCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTc                    >  2:8378483/1‑150 (MQ=255)
                                                                                                                              gcggCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTc                    <  1:11083783/150‑1 (MQ=255)
                                                                                                                                ggCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTcgc                  <  2:7939650/150‑1 (MQ=255)
                                                                                                                                   aGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGc               >  2:2135142/1‑150 (MQ=255)
                                                                                                                                     cgcCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGCAc             >  1:10618798/1‑150 (MQ=255)
                                                                                                                                      gcCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGCAcc            >  2:8554666/1‑150 (MQ=255)
                                                                                                                                      gcCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCGTGCCGGTGGCGGTCTTGGTCAGTTCGGTAAGCGTCACATGACGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGCAcc            <  2:5870742/150‑1 (MQ=255)
                                                                                                                                          tCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGCACCAGCg        >  1:5475781/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TCAGGGTGAAACGGTTTCGGCGCGCCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCTGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGCACCAGCGGACCAG  >  NC_008463/3198294‑3198587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑