BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	3,387,941	A→C	M67L (ATG→CTG)	cynS →	cyanase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	3,387,941	0	A	C	99.8%	1747.5 / NA	461	M67L (ATG→CTG)	cynS	cyanase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base C (161/299); minor base G (1/0); total (162/299)
Fisher's exact test for biased strand distribution p-value = 3.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 461 total aligned reads displayed.

GTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTATCGCCCTGACATTAGTCACCGCCGCCATGTTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCATGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTCTACGGCAGCACCCTGAAGGCACTGGTCCACGAACAG  >  NC_008463/3387794‑3388087

gTGGTCCTGAACACGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTg                                                                                                                                                  <  1:3655893/150‑1 (MQ=255)

gTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTg                                                                                                                                                  >  2:10423495/1‑150 (MQ=255)

gTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTg                                                                                                                                                  >  1:3968293/1‑150 (MQ=255)

  ggTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGga                                                                                                                                                <  2:1254063/150‑1 (MQ=255)

   gTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGgac                                                                                                                                               >  1:9320871/1‑150 (MQ=255)

    tCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGTTCTGGCCGACGGCACCGGTATCGACCTGACCTTCGTCACCGCCGCCATGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGgacg                                                                                                                                              <  2:1820875/150‑1 (MQ=255)

    tCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGgacg                                                                                                                                              >  1:4995327/1‑150 (MQ=255)

     ccTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGgccga                                                                                                                                             >  2:3914344/1‑150 (MQ=255)

        gAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGgacgacga                                                                                                                                          <  2:8390511/150‑1 (MQ=255)

          acacGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGacgacgacg                                                                                                                                        <  1:10979708/150‑1 (MQ=255)

           cAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGAcgc                                                                                                                                       >  1:1595262/1‑150 (MQ=255)

           cAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGAcgc                                                                                                                                       >  1:7049945/1‑150 (MQ=255)

           cAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCGTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGAcgc                                                                                                                                       <  2:10976253/150‑1 (MQ=255)

             aGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCgg                                                                                                                                     >  2:1631069/1‑150 (MQ=255)

               gCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTg                                                                                                                                   <  1:597540/150‑1 (MQ=255)

               gCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTg                                                                                                                                   <  2:2711410/150‑1 (MQ=255)

                   gCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTcc                                                                                                                               <  2:9741072/150‑1 (MQ=255)

                   gCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTcc                                                                                                                               <  2:5981875/150‑1 (MQ=255)

                    ccTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCct                                                                                                                              <  1:4531002/150‑1 (MQ=255)

                            tGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCaaa                                                                                                                      <  2:10846864/150‑1 (MQ=255)

                            tGTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCaaa                                                                                                                      <  2:6242339/150‑1 (MQ=255)

                             gTCCTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAg                                                                                                                     >  2:2293279/1‑150 (MQ=255)

                               ccTGGCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCa                                                                                                                   >  2:6671784/1‑150 (MQ=255)

                                  ggCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATcc                                                                                                                >  1:8635070/1‑150 (MQ=255)

                                   gCAGGATCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATccc                                                                                                               <  1:6607361/150‑1 (MQ=255)

                                        aTCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTgc                                                                                                          <  1:6895286/150‑1 (MQ=255)

                                         tCTGGCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGTCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTgcg                                                                                                         >  2:7994038/1‑150 (MQ=255)

                                           tGGCCGACGGCTCCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGGGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGcgcg                                                                                                       <  2:505111/150‑1 (MQ=255)

                                            ggCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCgg                                                                                                      <  1:2429893/150‑1 (MQ=255)

                                            ggCCGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCgg                                                                                                      <  2:9511894/150‑1 (MQ=255)

                                              ccGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGgca                                                                                                    <  1:5635146/150‑1 (MQ=255)

                                              ccGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGgca                                                                                                    <  2:10085608/150‑1 (MQ=255)

                                              ccGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGgca                                                                                                    <  1:6778966/150‑1 (MQ=255)

                                              ccGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGgca                                                                                                    <  1:2901355/150‑1 (MQ=255)

                                               cGACGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGgcag                                                                                                   <  2:9279344/150‑1 (MQ=255)

                                                  cGGCACCGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCAt                                                                                                <  1:10364847/150‑1 (MQ=255)

                                                       ccGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCcg                                                                                           <  1:7711957/150‑1 (MQ=255)

                                                        cggactCGCCCTGACCTACGACACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCcgg                                                                                          <  2:8725743/145‑1 (MQ=255)

                                                        cGGTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCcgg                                                                                          <  2:5179284/150‑1 (MQ=255)

                                                         ggTCTCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCcggc                                                                                         <  1:8255593/150‑1 (MQ=255)

                                                           tctCGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCcggcgg                                                                                       >  1:4888067/3‑150 (MQ=255)

                                                             tCGCCCAGTCCTTCGTCACCGCCGCCCTGCTCGGCCCGCAAGCCCTGCCAGAGGCGGCGGCGCGCCACGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCAGTCCCggcggcg                                                                                     >  2:8294126/1‑149 (MQ=255)

                                                              cGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCAt                                                                                    <  2:3363592/150‑1 (MQ=255)

                                                              cGCCCTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGACCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCAGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCAt                                                                                    >  1:238405/1‑150 (MQ=255)

                                                                cccTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTc                                                                                  <  1:9143830/150‑1 (MQ=255)

                                                                cccTGACCTTCGTCACCGCCGCCCTGCTCGGACATCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCATGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTc                                                                                  <  2:2849479/150‑1 (MQ=255)

                                                                 ccTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTcc                                                                                 <  1:6319923/150‑1 (MQ=255)

                                                                 ccTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTcc                                                                                 <  1:4196385/150‑1 (MQ=255)

                                                                 ccTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTcc                                                                                 <  2:1865210/150‑1 (MQ=255)

                                                                  cTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTccc                                                                                <  1:3375489/150‑1 (MQ=255)

                                                                  cTGACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTccc                                                                                <  2:2066054/150‑1 (MQ=255)

                                                                    gACCTTCGTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCAGTCCCAg                                                                              >  2:3100968/1‑150 (MQ=255)

                                                                     accttcgTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGc                                                                             >  1:3177145/7‑150 (MQ=255)

                                                                          cgTCACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGcgaccc                                                                        <  1:1530690/149‑1 (MQ=255)

                                                                             cACCGCCGCCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGcgacccgac                                                                     >  1:2744713/1‑150 (MQ=255)

                                                                                cgccgcCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCAt                                                                  <  2:6588850/150‑1 (MQ=255)

                                                                                cgccgcCCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCAt                                                                  <  1:10695238/150‑1 (MQ=255)

                                                                                  ccgccCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCt                                                                >  2:1385664/1‑150 (MQ=255)

                                                                                   cgccCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTa                                                               >  2:8815702/1‑150 (MQ=255)

                                                                                    gccCTGCTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTAc                                                              <  1:10017068/150‑1 (MQ=255)

                                                                                          ctCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTc                                                        <  1:62267/149‑1 (MQ=255)

                                                                                          ctCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTc                                                        <  1:7519429/149‑1 (MQ=255)

                                                                                           tCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCt                                                       >  2:5288090/1‑150 (MQ=255)

                                                                                               ccAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACga                                                   <  1:2525635/150‑1 (MQ=255)

                                                                                                 aGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACgaga                                                 <  1:9069335/150‑1 (MQ=255)

                                                                                                 aGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACgaga                                                 <  2:7155397/150‑1 (MQ=255)

                                                                                                  gCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGAt                                                <  1:8449136/150‑1 (MQ=255)

                                                                                                  gCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGAt                                                <  2:673504/150‑1 (MQ=255)

                                                                                                   cATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGAtg                                               <  2:4752637/150‑1 (MQ=255)

                                                                                                     tGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGAtgct                                             >  2:3694253/1‑150 (MQ=255)

                                                                                                      gCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGAtgcgg                                            <  1:9756468/150‑3 (MQ=255)

                                                                                                         cTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCaa                                         >  1:2221205/1‑150 (MQ=255)

                                                                                                          tGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAg                                        <  1:1207127/150‑1 (MQ=255)

                                                                                                           gCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGt                                       <  2:7457788/150‑1 (MQ=255)

                                                                                                              agagGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTCTa                                    >  2:9051885/1‑150 (MQ=255)

                                                                                                               gagGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGTCGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTCTAc                                   <  1:9975557/150‑1 (MQ=255)

                                                                                                                agGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTCTACg                                  <  1:572868/150‑1 (MQ=255)

                                                                                                                 ggcggcggcGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTCCCGCTTCTACGAGATGCTGCAAGTCTACgg                                 <  1:5351239/150‑1 (MQ=255)

                                                                                                                 ggcggcggcGCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCAGTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTCTACgg                                 <  1:10198006/150‑1 (MQ=255)

                                                                                                                  gcggcggcgCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTCTACGgc                                <  1:192678/150‑1 (MQ=255)

                                                                                                                  gcggcggcgCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTCTACGgc                                <  1:7289139/150‑1 (MQ=255)

                                                                                                                   cggcggcgCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTCTACGgca                               <  2:6438605/150‑1 (MQ=255)

                                                                                                                    ggcggcgCGCAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTCTACGgcag                              >  2:1509042/1‑150 (MQ=255)

                                                                                                                        gcgcgcAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTCTACGGCAGCAcc                          >  2:3726699/1‑150 (MQ=255)

                                                                                                                        gcgcgcAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTCTACGGCAGCAcc                          <  1:7877805/150‑1 (MQ=255)

                                                                                                                         cgcgcAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTCTACGGCAGCAccc                         <  2:4827807/150‑1 (MQ=255)

                                                                                                                             cAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTCTACGGCAGCACCCTGaa                     <  2:5665956/150‑1 (MQ=255)

                                                                                                                             cAAGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTCTACGGCAGCACCCTGaa                     <  2:170928/150‑1 (MQ=255)

                                                                                                                              aaGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTCTACGGCAGCACCCTGAAg                    >  1:3831204/1‑150 (MQ=255)

                                                                                                                               aGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCTAGTCTACGGCAGCACCCTGAAgg                   <  2:4380634/150‑1 (MQ=255)

                                                                                                                               aGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTCTACGGCAGCACCCTGAAgg                   <  2:1159690/150‑1 (MQ=255)

                                                                                                                               aGGTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTCTACGGCAGCACCCTGAAgg                   <  2:2368481/150‑1 (MQ=255)

                                                                                                                                 gTCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTCTACGGCAGCACCCTGAAGGCa                 >  1:10487159/1‑150 (MQ=255)

                                                                                                                                  tCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTCTACGGCAGCACCCTGAAGGCAc                <  2:6838189/150‑1 (MQ=255)

                                                                                                                                  tCGCCGCCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTCTACGGCAGCACCCTGAAGGCAc                <  2:5715865/150‑1 (MQ=255)

                                                                                                                                   cgccgcCCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTCTACGGCAGCACCCTGAAGGCACt               >  2:7834087/1‑150 (MQ=255)

                                                                                                                                    gccgccCAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTCTACGGCAGCACCCTGAAGGCACTg              <  1:9510088/150‑1 (MQ=255)

                                                                                                                                        cccAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTCTACGGCAGCACCCTGAAGGCACTGGTcc          <  1:8731709/150‑1 (MQ=255)

                                                                                                                                         ccAACTCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTCTACGGCAGCACCCTGAAGGCACTGGTCCa         <  1:2268881/150‑1 (MQ=255)

                                                                                                                                              tCGGCCTGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTCTACGGCAGCACCCTGAAGGCACTGGTCCACGAAc    <  1:8876108/150‑1 (MQ=255)

GTGGTCCTGAACAAGGCCCGCCTGGGCCTGTCCTGGCAGGATCTGGCCGACGGCACCGGTATCGCCCTGACATTAGTCACCGCCGCCATGTTCGGCCAGCATGCCCTGCCAGAGGCGGCGGCGCGCAAGGTCGCCGCCCAACTCGGCATGGACGACGACGCGGTGCTCCTGCTGCAAAGCATCCCGCTGCGCGGCAGCATTCCCGGCGGCATTCCCAGCGACCCGACCATCTACCGCTTCTACGAGATGCTGCAAGTCTACGGCAGCACCCTGAAGGCACTGGTCCACGAACAG  >  NC_008463/3387794‑3388087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`