Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 4,565,005 C→G G319A (GGG→GCG)  PA14_RS20885 ← anthranilate synthase component 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084634,565,0050CG99.8% 2312.8 / NA 601G319A (GGG→GCG) PA14_RS20885anthranilate synthase component 1
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base G (289/311);  total (289/312)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 601 total aligned reads displayed.

GCATCATGTGCTCGGCGATCTCCTTGGCGTCCAGGCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCCCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCAAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCCCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGC  >  NC_008463/4564859‑4565151
                                                                                                                                                  |                                                                                                                                                  
  atcatGTGCTCGGCGATCTCCTTGGCGTCCAGGCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGcgtcg                                                                                                                                               <  1:7017796/150‑1 (MQ=255)
   tcatGTGCTCGGCGATCTCCTTGGCGTCCAGGCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGcgtcgt                                                                                                                                              <  1:9606204/150‑1 (MQ=255)
      tgtgCTCGGCGATCTCCTTGGCGTCCAGGCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACt                                                                                                                                           >  2:8468654/1‑150 (MQ=255)
       gtgCTCGGCGATCTCCTTGGCGTCCAGGCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACtt                                                                                                                                          <  1:9247005/150‑1 (MQ=255)
         gCTCGGCGATCTCCTTGGCGTCCAGGCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCa                                                                                                                                        >  1:498615/1‑150 (MQ=255)
              gCGATCTCCTTGGCGTCCAGGCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGcc                                                                                                                                   <  2:5545880/150‑1 (MQ=255)
                gATCTCCTTGGCGTCCAGGCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGa                                                                                                                                 <  2:6161240/150‑1 (MQ=255)
                 aTCTCCTTGGCGTCCAGGCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGAc                                                                                                                                >  1:1214772/1‑150 (MQ=255)
                    tcCTTGGCGTCCAGGCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTcc                                                                                                                             <  1:1397024/150‑1 (MQ=255)
                     ccTTGGCGTCCAGGCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCg                                                                                                                            >  1:2976381/1‑150 (MQ=255)
                      cTTGGCGTCCAGGCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCgg                                                                                                                           <  1:8632617/150‑1 (MQ=255)
                      cTTGGCGTCCAGGCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCgg                                                                                                                           <  2:727176/150‑1 (MQ=255)
                         ggCGTCCAGGCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGa                                                                                                                        >  2:5128388/1‑150 (MQ=255)
                            gTCCAGGCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAgc                                                                                                                     >  1:7732358/1‑150 (MQ=255)
                             tCCAGGCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAgcg                                                                                                                    >  2:1942679/1‑150 (MQ=255)
                               cAGGCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGcc                                                                                                                  >  2:2216486/1‑150 (MQ=255)
                               cAGGCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGcc                                                                                                                  <  1:4614054/150‑1 (MQ=255)
                                 ggCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGccga                                                                                                                >  1:8497705/1‑148 (MQ=255)
                                  gcgcAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCgaa                                                                                                               <  1:330577/150‑1 (MQ=255)
                                   cgcAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAAc                                                                                                              <  1:2491388/150‑1 (MQ=255)
                                     cAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAg                                                                                                            >  2:8312426/1‑150 (MQ=255)
                                       aCTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGc                                                                                                          >  2:4551026/1‑150 (MQ=255)
                                       aCTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGc                                                                                                          >  1:9173810/1‑150 (MQ=255)
                                       aCTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGc                                                                                                          >  2:326219/1‑150 (MQ=255)
                                        cTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCa                                                                                                         >  1:6263178/1‑150 (MQ=255)
                                        cTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCa                                                                                                         >  2:3645938/1‑150 (MQ=255)
                                         tCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCTATGGGGTAGAGTTACACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAg                                                                                                        <  2:2854081/150‑1 (MQ=255)
                                           cGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGaa                                                                                                      <  1:10847901/150‑1 (MQ=255)
                                             cTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGt                                                                                                    >  2:4214850/1‑150 (MQ=255)
                                              ttCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCACTGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCCGACGTc                                                                                                   >  2:9258279/1‑150 (MQ=255)
                                              ttCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTc                                                                                                   >  1:5524656/1‑150 (MQ=255)
                                               tCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTcc                                                                                                  >  2:5896987/1‑150 (MQ=255)
                                               tCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTcc                                                                                                  >  1:6824467/1‑150 (MQ=255)
                                                 cAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTcccc                                                                                                >  1:2638833/1‑150 (MQ=255)
                                                     cGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCa                                                                                            <  1:10012721/150‑1 (MQ=255)
                                                          gTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAg                                                                                       >  2:10998442/1‑150 (MQ=255)
                                                          gTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAg                                                                                       <  2:4088682/150‑1 (MQ=255)
                                                          gTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAg                                                                                       <  1:1157595/150‑1 (MQ=255)
                                                           tCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAgg                                                                                      <  1:1813910/150‑1 (MQ=255)
                                                            ccAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGga                                                                                     <  1:5764268/150‑1 (MQ=255)
                                                             cAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGgaa                                                                                    <  1:796593/150‑1 (MQ=255)
                                                               gTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGgaaga                                                                                  <  2:4892037/150‑1 (MQ=255)
                                                                 tCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGaagaag                                                                                >  1:10674581/1‑150 (MQ=255)
                                                                  ccGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGc                                                                               >  1:6153236/1‑150 (MQ=255)
                                                                      aTCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTa                                                                           >  2:8889388/1‑150 (MQ=255)
                                                                      aTCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTa                                                                           <  1:6012933/150‑1 (MQ=255)
                                                                       tCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCACTGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTAc                                                                          >  2:3524353/1‑150 (MQ=255)
                                                                         gATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACgg                                                                        >  1:6622237/1‑150 (MQ=255)
                                                                          aTGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACggg                                                                       <  2:336782/150‑1 (MQ=255)
                                                                          aTGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACggg                                                                       >  2:4626631/1‑150 (MQ=255)
                                                                          aTGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACggg                                                                       >  2:442425/1‑150 (MQ=255)
                                                                           tGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGc                                                                      >  2:10855729/1‑150 (MQ=255)
                                                                              cgcCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTgg                                                                   <  2:6283776/150‑1 (MQ=255)
                                                                              cgcCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTgg                                                                   <  1:9004811/150‑1 (MQ=255)
                                                                                ccccGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTgggg                                                                 <  2:5420584/150‑1 (MQ=255)
                                                                                ccccGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTgggg                                                                 >  2:776481/1‑150 (MQ=255)
                                                                                  ccGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGtt                                                               <  2:1129053/150‑1 (MQ=255)
                                                                                   cGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCACTGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTg                                                              <  1:1577797/150‑1 (MQ=255)
                                                                                   cGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTg                                                              <  1:3857100/150‑1 (MQ=255)
                                                                                    gggCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGc                                                             >  1:2151895/1‑150 (MQ=255)
                                                                                    gggCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGc                                                             >  2:7087453/1‑150 (MQ=255)
                                                                                       cATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGGTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCa                                                          <  2:2325545/150‑1 (MQ=255)
                                                                                        aTCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAg                                                         <  1:5245013/150‑1 (MQ=255)
                                                                                        aTCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAg                                                         >  1:430495/1‑150 (MQ=255)
                                                                                          cgcgTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGc                                                       >  2:1297955/1‑150 (MQ=255)
                                                                                          cgcgTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGc                                                       <  1:9935450/150‑1 (MQ=255)
                                                                                                cgcgcgGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACt                                                 >  1:1014198/1‑150 (MQ=255)
                                                                                                    cgGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCg                                             >  2:7650827/1‑150 (MQ=255)
                                                                                                      ggCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGACGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGAt                                           >  2:9088597/1‑150 (MQ=255)
                                                                                                      ggCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGAt                                           <  2:7006619/150‑1 (MQ=255)
                                                                                                         gAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAgg                                        >  2:10676814/1‑150 (MQ=255)
                                                                                                           gTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGcc                                      <  2:10506868/150‑1 (MQ=255)
                                                                                                            tGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGccc                                     <  2:9365419/150‑1 (MQ=255)
                                                                                                                ggCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCa                                 >  1:3557257/1‑150 (MQ=255)
                                                                                                                 gCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAg                                <  2:1559244/150‑1 (MQ=255)
                                                                                                                 gCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAg                                >  2:9011387/1‑150 (MQ=255)
                                                                                                                   aaTGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAggg                              >  2:3006638/1‑150 (MQ=255)
                                                                                                                      ggggTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTc                           >  2:959198/1‑150 (MQ=255)
                                                                                                                       gggTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTcc                          >  2:9820241/1‑150 (MQ=255)
                                                                                                                       gggTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTcc                          >  1:983240/1‑150 (MQ=255)
                                                                                                                        ggTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCcg                         >  2:918858/1‑150 (MQ=255)
                                                                                                                           agagTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCgcgc                      >  1:4143367/1‑150 (MQ=255)
                                                                                                                           agagTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCgcgc                      <  2:56632/150‑1 (MQ=255)
                                                                                                                             agTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCAc                    >  1:4514522/1‑150 (MQ=255)
                                                                                                                              gTTCCACCTCGCGACTCTCCGCGTCGTACATCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGACCGCCAGGGGTCCGCGCACg                   >  2:1700501/1‑150 (MQ=255)
                                                                                                                                tCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGc                 >  2:7426951/1‑150 (MQ=255)
                                                                                                                                    ccTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGc             <  1:7497740/150‑1 (MQ=255)
                                                                                                                                    ccTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCGGGGGTTGCGCAGGCACAACAGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATga             >  2:6570818/1‑149 (MQ=255)
                                                                                                                                     cTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCt            >  2:10104116/1‑150 (MQ=255)
                                                                                                                                     cTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCt            <  2:10357387/150‑1 (MQ=255)
                                                                                                                                      tCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTg           <  1:4771303/150‑1 (MQ=255)
                                                                                                                                      tCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTg           >  2:7963944/1‑150 (MQ=255)
                                                                                                                                        gcgACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGaa         <  2:6431294/150‑1 (MQ=255)
                                                                                                                                        gcgACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGaa         <  1:2341688/150‑1 (MQ=255)
                                                                                                                                        gcgACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGaa         <  1:8145889/150‑1 (MQ=255)
                                                                                                                                         cgACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAg        >  1:9499276/1‑150 (MQ=255)
                                                                                                                                           aCTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTTAGGGCTAGGGTTGCGCAGGCACTACTGGCGATAGGCCCGCCCGGGGTCCGCGCACGGCAGGCGGAAGcg      >  2:2601546/1‑149 (MQ=255)
                                                                                                                                           aCTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCt      >  1:539890/1‑150 (MQ=255)
                                                                                                                                            ctctCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTg     <  1:10349174/150‑1 (MQ=255)
                                                                                                                                              ctcCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTgcg   <  2:3802109/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GCATCATGTGCTCGGCGATCTCCTTGGCGTCCAGGCGCAACTCCGCTTCCAGGCGATTGTCCAGTTCCGCATCGATGGCGCCCCGGGCATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCCCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCAAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCCCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGC  >  NC_008463/4564859‑4565151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑