Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,149,425 T→C Q549Q (CAA→CAG)  PA14_RS09965 ← M14 family metallopeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,149,4250TC100.0% 1711.8 / NA 451Q549Q (CAA→CAG) PA14_RS09965M14 family metallopeptidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (220/231);  total (220/231)

Only 100 of 451 total aligned reads displayed.

GCGCAGGCGTTTGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTTTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGCCCGGAATCCGGTT  >  NC_008463/2149278‑2149571
                                                                                                                                                   |                                                                                                                                                  
 cgcAGGCGTTTGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGc                                                                                                                                                 <  1:10063484/150‑1 (MQ=255)
 cgcAGGCGTTTGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGc                                                                                                                                                 <  1:9278081/150‑1 (MQ=255)
  gcAGGCGTTTGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCg                                                                                                                                                >  1:8576169/1‑150 (MQ=255)
   cAGGCGTTTGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCgg                                                                                                                                               <  2:9755126/150‑1 (MQ=255)
   cAGGCGTTTGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCGTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCgg                                                                                                                                               <  1:5041351/150‑1 (MQ=255)
    aGGCGTTTGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGt                                                                                                                                              >  1:7351981/1‑150 (MQ=255)
     ggCGTTTGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGtt                                                                                                                                             >  1:8934482/1‑150 (MQ=255)
      gCGTTTGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTg                                                                                                                                            <  2:10338133/150‑1 (MQ=255)
      gCGTTTGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTg                                                                                                                                            >  1:430042/1‑150 (MQ=255)
      gCGTTTGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTg                                                                                                                                            <  1:1076398/150‑1 (MQ=255)
      gCGTTTGGCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTCTACCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTg                                                                                                                                            <  2:8199801/150‑1 (MQ=255)
       cGTTTGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTgc                                                                                                                                           >  1:6657156/1‑150 (MQ=255)
         tttGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGATGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTgcag                                                                                                                                         <  1:5304875/150‑1 (MQ=255)
         tgtGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTgcag                                                                                                                                         <  1:2297662/148‑1 (MQ=255)
          ttGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTgcagc                                                                                                                                        <  2:888529/150‑1 (MQ=255)
          ttGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTgcagc                                                                                                                                        <  1:4140909/150‑1 (MQ=255)
             tCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAgg                                                                                                                                     >  2:10466762/1‑150 (MQ=255)
             tCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAgg                                                                                                                                     <  1:8119283/150‑1 (MQ=255)
                aaGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCg                                                                                                                                  >  1:4433770/1‑150 (MQ=255)
                aaGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCg                                                                                                                                  >  2:4982644/1‑150 (MQ=255)
                 aGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGa                                                                                                                                 <  1:1365278/150‑1 (MQ=255)
                 aGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGa                                                                                                                                 <  2:7193889/150‑1 (MQ=255)
                  ggCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGAt                                                                                                                                <  1:8768371/150‑1 (MQ=255)
                  ggCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGAt                                                                                                                                <  2:3681350/150‑1 (MQ=255)
                   gCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGACTTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATg                                                                                                                               >  1:398352/1‑150 (MQ=255)
                   gCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGATTCCGGACTCTGCGGTTGCAGCAGGTCGATg                                                                                                                               <  2:1639783/150‑1 (MQ=255)
                   gCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATg                                                                                                                               >  2:8605383/1‑150 (MQ=255)
                     ggACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCAACGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGc                                                                                                                             <  2:668659/150‑1 (MQ=255)
                      gACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCTGGTCGATGGcc                                                                                                                            >  2:9596625/1‑150 (MQ=255)
                      gACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGcc                                                                                                                            >  2:3260079/1‑150 (MQ=255)
                      gACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGcc                                                                                                                            <  2:6147253/150‑1 (MQ=255)
                      gACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGcc                                                                                                                            <  1:11093277/150‑1 (MQ=255)
                        cTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAg                                                                                                                          <  2:827694/150‑1 (MQ=255)
                         ttCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAgg                                                                                                                         >  1:7673692/1‑150 (MQ=255)
                         ttCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAgg                                                                                                                         >  2:581173/1‑150 (MQ=255)
                                  gggTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGc                                                                                                                >  2:5387485/1‑150 (MQ=255)
                                  gggTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGc                                                                                                                <  2:1980335/150‑1 (MQ=255)
                                     tCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGc                                                                                                             <  1:6548592/150‑1 (MQ=255)
                                      cTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCt                                                                                                            <  1:8308122/150‑1 (MQ=255)
                                       ttCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTg                                                                                                           <  1:11029398/150‑1 (MQ=255)
                                       ttCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTg                                                                                                           <  1:967139/150‑1 (MQ=255)
                                            ccAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCgg                                                                                                      >  2:3319979/1‑150 (MQ=255)
                                            ccAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCgg                                                                                                      <  1:5457950/150‑1 (MQ=255)
                                            ccAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCgg                                                                                                      <  2:10688492/150‑1 (MQ=255)
                                               gCATCTTGCGCGCCCTCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGACGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGgtgt                                                                                                   <  1:2824490/150‑1 (MQ=255)
                                               gCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGTAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATTGCCAGGACGCCCAGCGGCTGGGCGgtgt                                                                                                   <  2:2825244/150‑1 (MQ=255)
                                               gCATCGTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGgtgt                                                                                                   <  2:10792655/150‑1 (MQ=255)
                                                 aTCTTGCGCGCCATCGGCTCCATCACGTCTTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGtcg                                                                                                 <  2:1558417/150‑1 (MQ=255)
                                                  tCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGtcga                                                                                                >  1:1689958/1‑150 (MQ=255)
                                                     tGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGtcgatcg                                                                                             >  2:4820245/1‑150 (MQ=255)
                                                     tGCGCGCCATCGGCTCCATCACGTATACCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGTAGAAGCCCCAGGACCATAACGAATACGGACTCTGCGTTTGCAGCAGGTCGATGGCCATGACTCCCAGCGGCTGGGCGGTGtcgatcg                                                                                             >  1:3620469/1‑150 (MQ=255)
                                                       cgcgcCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGAc                                                                                           >  2:6983576/1‑150 (MQ=255)
                                                        gcgcCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCCGGGTGGTATTGAAGAAGCCCCCGGCCCAGAACGTCTCCGGACGCTCCGGTTGCAGCTGGTCGCTCGCCCGGACGCAGCGCGGCTGGGCGGTGtcgatcgaga                                                                                          >  2:7607848/1‑148 (MQ=255)
                                                        gcgcCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCCGGGTGGAATTGAAGAAGCCCCCGGACCAGAACGAAGCCGGACTCTGGGGTTGCCGCAGGGCGCTGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACa                                                                                          >  2:7695170/1‑150 (MQ=255)
                                                        gcgcCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGGGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACa                                                                                          >  2:4705027/1‑150 (MQ=255)
                                                           ccATCCGCTCCATCCCGTCGTCCTCCGGCCCCACGGCGGCCACCAGGGTGGAATAGAAGCAGCCCCAGGACCAGAACCAAACCGGACTCGGCGGTTGCAGCAGGCCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCg                                                                                       <  2:1757521/150‑1 (MQ=255)
                                                            cATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGa                                                                                      <  1:6220196/150‑1 (MQ=255)
                                                             aTCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAg                                                                                     >  1:597762/1‑150 (MQ=255)
                                                              tCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAgc                                                                                    <  1:10603178/150‑1 (MQ=255)
                                                                ggCTCCATCACGGCTTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGCATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAgccg                                                                                  <  2:2378752/150‑1 (MQ=255)
                                                                 gCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAgccgg                                                                                 <  1:7088883/150‑1 (MQ=255)
                                                                 gCTCCATCACGTAGTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGCTCGACAGCGAgccgg                                                                                 <  2:9326610/150‑1 (MQ=255)
                                                                  cTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAgccggc                                                                                <  2:10867648/150‑1 (MQ=255)
                                                                   tCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAgccggcc                                                                               <  2:10004598/150‑1 (MQ=255)
                                                                    ccATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAgccggccg                                                                              >  1:9472311/1‑150 (MQ=255)
                                                                       tcccGTAGTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGACGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGa                                                                           <  2:6349434/147‑1 (MQ=255)
                                                                       tCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGa                                                                           >  1:1496161/1‑150 (MQ=255)
                                                                                cctccGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCa                                                                  >  2:3490644/1‑150 (MQ=255)
                                                                                   ccGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAgc                                                               <  2:7781609/150‑1 (MQ=255)
                                                                                      gCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCt                                                            <  2:6939141/150‑1 (MQ=255)
                                                                                           tcGGCGGCCACCAGGGTGGAATAGAAGAAGCCCCAGGACCAGAACGACTCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAAc                                                       <  2:802361/150‑1 (MQ=255)
                                                                                              gcggcCACCAGGGTGGAATTGAAGAAGCCCCAGGACCCGAACGAATCCGGACTCTGCGGGTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGc                                                    <  2:10216558/150‑1 (MQ=255)
                                                                                                 gcCACCCGCGTGGCATTGCAGAACCCCCCGCACCAGCACCACACCGGACACTGCGGTTGCAGCAGGTCGATGGCCAGCACGCCCAGCGGCTGGGCGGTGCCGAACGACAGCGAGCCGGCCGGGAAGGTCCGCAAGCGCTGGAACGGCGTc                                                 <  2:5627983/150‑1 (MQ=255)
                                                                                                    accaGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGc                                              >  2:1241480/1‑150 (MQ=255)
                                                                                                    accaGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGc                                              >  1:7533047/1‑150 (MQ=255)
                                                                                                        gggTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCCGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCACGCGCTGGAACGGCTTCGGCGTGc                                          >  2:293085/1‑150 (MQ=255)
                                                                                                        gggTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGc                                          >  2:3979275/1‑150 (MQ=255)
                                                                                                        gggTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGc                                          >  2:2174592/1‑150 (MQ=255)
                                                                                                         ggTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGcc                                         >  1:6332170/1‑150 (MQ=255)
                                                                                                          gTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCg                                        >  2:4463116/1‑150 (MQ=255)
                                                                                                               aTTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGAc                                   <  1:5679419/150‑1 (MQ=255)
                                                                                                               aTTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGAc                                   <  1:7939414/150‑1 (MQ=255)
                                                                                                                ttGAAGACGCCCCAGGACCAGATCGAAACCGGACTCTGCGGTTGCAGCAGGTCGCTGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGAcc                                  <  2:5466758/150‑1 (MQ=255)
                                                                                                                  gaagaaGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACcag                                >  1:4860694/1‑150 (MQ=255)
                                                                                                                    agaagCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACcagca                              >  2:3841397/1‑150 (MQ=255)
                                                                                                                         ccccAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCGTCGGCGTGCCGCTGCCCAGCGGGCGa                         >  2:6834896/1‑150 (MQ=255)
                                                                                                                           ccAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGAcc                       <  1:10133231/150‑1 (MQ=255)
                                                                                                                            cAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCt                      <  2:3369100/150‑1 (MQ=255)
                                                                                                                             aGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCtt                     <  1:9941164/150‑1 (MQ=255)
                                                                                                                             aGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCGTCGGCGTGCCGCTGACCAGCAGGCGACCtt                     >  1:3494362/1‑150 (MQ=255)
                                                                                                                              ggACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTc                    >  1:9156433/1‑150 (MQ=255)
                                                                                                                              ggACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTc                    <  2:4210107/150‑1 (MQ=255)
                                                                                                                                aCCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGt                  >  2:7247548/1‑150 (MQ=255)
                                                                                                                                aCCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGt                  >  2:2120017/1‑150 (MQ=255)
                                                                                                                                      aCGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGACGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGCCCg            >  2:2285997/1‑150 (MQ=255)
                                                                                                                                         aaTCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGCCCGGaa         >  1:1610710/1‑150 (MQ=255)
                                                                                                                                           tCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGCCCGGAATc       >  2:9144541/1‑150 (MQ=255)
                                                                                                                                             cGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGCCCGGAATCCg     >  2:9431807/1‑150 (MQ=255)
                                                                                                                                              ggACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGCCCGGAATCCgg    >  1:1823663/1‑150 (MQ=255)
                                                                                                                                                aCTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGCCCGGAATCCGGtt  <  1:2518459/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCGCAGGCGTTTGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTTTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGCCCGGAATCCGGTT  >  NC_008463/2149278‑2149571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑