Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,407,463 A→G K125E (AAG→GAG)  PA14_RS11255 → glutathione S‑transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,407,4630AG100.0% 1796.0 / NA 459K125E (AAG→GAG) PA14_RS11255glutathione S‑transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (210/249);  total (210/249)

Only 100 of 463 total aligned reads displayed.

CGCTATGCGCCGGGCGACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACCCTGCGTACGCCGCCGGAGCAACGCGACAAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTCATCTACGCCTGGTTCGAG  >  NC_008463/2407316‑2407609
                                                                                                                                                   |                                                                                                                                                  
  cTATGCGCCGGGCGACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGc                                                                                                                                                <  1:2287826/150‑1 (MQ=255)
     tGCGCCGGGCGACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCt                                                                                                                                             >  1:1013976/1‑150 (MQ=255)
         ccGGGCGACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATc                                                                                                                                         >  2:8339400/1‑150 (MQ=255)
             gCGACCTCTCTCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCaa                                                                                                                                     <  2:5791746/150‑1 (MQ=255)
             gCGACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCaa                                                                                                                                     >  1:2097659/1‑150 (MQ=255)
              cGACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCaaa                                                                                                                                    <  2:2592379/150‑1 (MQ=255)
               gACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAg                                                                                                                                   >  1:9481224/1‑150 (MQ=255)
                   tctATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGgcgc                                                                                                                               <  1:1652411/150‑1 (MQ=255)
                    ctATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCt                                                                                                                              <  2:8542968/150‑1 (MQ=255)
                          gCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGAc                                                                                                                        >  2:10623326/1‑150 (MQ=255)
                           cAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGAcc                                                                                                                       <  2:4707273/150‑1 (MQ=255)
                             ggACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTg                                                                                                                     >  2:5366619/1‑150 (MQ=255)
                              gACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTgc                                                                                                                    <  1:190110/150‑1 (MQ=255)
                              gACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTgc                                                                                                                    >  1:5552332/1‑150 (MQ=255)
                              gACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTgc                                                                                                                    <  1:5739466/150‑1 (MQ=255)
                                cccGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGACCGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTgcgg                                                                                                                  >  1:524839/1‑150 (MQ=255)
                                 ccGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTgcggc                                                                                                                 >  1:10587815/1‑150 (MQ=255)
                                   ggTGCGCCGCGCCGACGCCGACATGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGTCCTGTTCTGTGGAACGGTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCATTGGCGCTGCAGACCTGCGGCGa                                                                                                               <  2:2372494/150‑1 (MQ=255)
                                    gTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAg                                                                                                              >  1:3237605/1‑150 (MQ=255)
                                     tGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGt                                                                                                             <  2:2853313/150‑1 (MQ=255)
                                         ccgcgccgACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTtgct                                                                                                         >  1:9703838/1‑150 (MQ=255)
                                            cgccgacgccgaCAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCg                                                                                                      <  2:9927502/150‑1 (MQ=255)
                                             gccgacgccgacAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCgg                                                                                                     >  1:4386577/1‑150 (MQ=255)
                                             gccgacgccgacAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCgg                                                                                                     >  1:5082200/1‑150 (MQ=255)
                                              ccgacgccgacAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCggg                                                                                                    <  2:4310791/150‑1 (MQ=255)
                                              ccgacgccgacAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCggg                                                                                                    >  2:3871817/1‑150 (MQ=255)
                                               cgacgccgacAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGt                                                                                                   >  1:8354363/1‑150 (MQ=255)
                                                   gccgacAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCg                                                                                               >  2:1787194/1‑150 (MQ=255)
                                                      gacAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGAc                                                                                            >  1:6529081/1‑150 (MQ=255)
                                                       acAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGAcc                                                                                           <  2:478130/150‑1 (MQ=255)
                                                          aGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGa                                                                                        >  1:6357978/1‑150 (MQ=255)
                                                          aGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGa                                                                                        >  2:341907/1‑150 (MQ=255)
                                                            tggatggaCTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGAcc                                                                                      >  2:408674/1‑150 (MQ=255)
                                                             ggatggaCTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGAccc                                                                                     <  1:3104696/150‑1 (MQ=255)
                                                                tggactggacCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTgg                                                                                  <  2:4892/150‑1 (MQ=255)
                                                                 ggactggacCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTggc                                                                                 >  1:7159280/1‑150 (MQ=255)
                                                                  gactggacCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTggcc                                                                                >  2:8057174/1‑150 (MQ=255)
                                                                    ctggacCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTggccgg                                                                              <  2:8967070/150‑1 (MQ=255)
                                                                         ccaccTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAAc                                                                         >  2:3925242/1‑150 (MQ=255)
                                                                           accTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAAccc                                                                       >  1:2546226/1‑150 (MQ=255)
                                                                            ccTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCt                                                                      <  1:7061475/150‑1 (MQ=255)
                                                                               cGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGc                                                                   <  2:7261428/150‑1 (MQ=255)
                                                                                gACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCt                                                                  <  1:6305886/150‑1 (MQ=255)
                                                                                 aCTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTg                                                                 <  2:1248676/150‑1 (MQ=255)
                                                                                 aCTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTg                                                                 >  1:2958678/1‑150 (MQ=255)
                                                                                      cGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCgg                                                            <  1:9052943/150‑1 (MQ=255)
                                                                                      cGCCGGGCCGGTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCgg                                                            <  1:4728637/150‑1 (MQ=255)
                                                                                       gCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGc                                                           >  1:5930673/1‑150 (MQ=255)
                                                                                        ccGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCg                                                          <  2:4379273/150‑1 (MQ=255)
                                                                                         cGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGa                                                         <  1:4861918/150‑1 (MQ=255)
                                                                                          gggCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCACCGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGaa                                                        <  2:3511946/150‑1 (MQ=255)
                                                                                          gggCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGaa                                                        >  1:3760945/1‑150 (MQ=255)
                                                                                              cGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCt                                                    >  2:11028071/1‑150 (MQ=255)
                                                                                               gTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCtt                                                   >  2:5235034/1‑150 (MQ=255)
                                                                                                ttCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTc                                                  <  1:9172629/150‑1 (MQ=255)
                                                                                                 tCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCg                                                 >  1:4060192/1‑150 (MQ=255)
                                                                                                  ccGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCgg                                                <  2:3768188/150‑1 (MQ=255)
                                                                                                   cgcgACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGc                                               <  2:6232354/150‑1 (MQ=255)
                                                                                                    gcgACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCa                                              <  2:7418848/150‑1 (MQ=255)
                                                                                                    gcgACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCa                                              >  2:9839790/1‑150 (MQ=255)
                                                                                                    gcgACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCa                                              >  1:7897233/1‑150 (MQ=255)
                                                                                                     cgACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCAt                                             <  2:10279538/150‑1 (MQ=255)
                                                                                                      gACCCGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGCTCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATg                                            <  1:4195252/150‑1 (MQ=255)
                                                                                                       aCCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATgg                                           <  2:658744/150‑1 (MQ=255)
                                                                                                       aCCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATgg                                           >  2:606844/1‑150 (MQ=255)
                                                                                                          tGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTg                                        >  2:6485461/1‑150 (MQ=255)
                                                                                                          tGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTg                                        <  2:10798386/150‑1 (MQ=255)
                                                                                                             tCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACa                                     <  2:5568472/150‑1 (MQ=255)
                                                                                                               tGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACAtt                                   <  1:1581180/150‑1 (MQ=255)
                                                                                                                 gggAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTcc                                 <  2:5170449/150‑1 (MQ=255)
                                                                                                                  ggAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTccc                                >  1:8547494/1‑150 (MQ=255)
                                                                                                                  ggAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTccc                                <  1:1407999/150‑1 (MQ=255)
                                                                                                                   gAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTcccc                               >  2:320134/1‑150 (MQ=255)
                                                                                                                   gAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTcccc                               <  1:5846108/150‑1 (MQ=255)
                                                                                                                     acgcTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTg                             >  2:6946763/4‑150 (MQ=255)
                                                                                                                       gcTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTggg                           <  1:6858636/149‑1 (MQ=255)
                                                                                                                       gcTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTggg                           >  1:6116110/2‑150 (MQ=255)
                                                                                                                       gcTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTggg                           <  2:10556827/149‑1 (MQ=255)
                                                                                                                         tGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCt                         >  2:308878/1‑150 (MQ=255)
                                                                                                                          gCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTg                        >  2:1494947/1‑150 (MQ=255)
                                                                                                                          gCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTg                        >  1:268516/1‑150 (MQ=255)
                                                                                                                           cGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGt                       <  1:3339104/150‑1 (MQ=255)
                                                                                                                           cGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGt                       <  1:8116542/150‑1 (MQ=255)
                                                                                                                           cGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGt                       <  1:8720233/150‑1 (MQ=255)
                                                                                                                                 ccgccgGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTCATc                 <  2:9516096/150‑1 (MQ=255)
                                                                                                                                  cgccgGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTCATCt                <  1:1126235/150‑1 (MQ=255)
                                                                                                                                   gccgGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTCATCTa               <  1:10285473/150‑1 (MQ=255)
                                                                                                                                   gccgGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTCATCTa               <  2:1683505/150‑1 (MQ=255)
                                                                                                                                    ccgGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTCATCTAc              <  1:5342893/150‑1 (MQ=255)
                                                                                                                                    ccgGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTCATCTAc              >  1:7620509/1‑150 (MQ=255)
                                                                                                                                      ggAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTCATCTACGc            >  1:131185/1‑150 (MQ=255)
                                                                                                                                          cAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTCATCTACGCCTgg        <  1:10048886/150‑1 (MQ=255)
                                                                                                                                           aaCGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTCATCTACGCCTGGt       <  1:7521806/150‑1 (MQ=255)
                                                                                                                                           aaCGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTCATCTACGCCTGGt       <  2:8777917/150‑1 (MQ=255)
                                                                                                                                           aaCGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTCATCTACGCCTGGt       <  1:181299/150‑1 (MQ=255)
                                                                                                                                              gcgACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTCATCTACGCCTGGTTCg    >  1:8058622/1‑150 (MQ=255)
                                                                                                                                               cgacgaGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTCATCTACGCCTGGTTCga   <  1:10792980/150‑1 (MQ=255)
                                                                                                                                               cgacgaGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTCATCTACGCCTGGTTCga   <  2:7630701/150‑1 (MQ=255)
                                                                                                                                               cgacgaGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTCATCTACGCCTGGTTCga   >  2:10047621/1‑150 (MQ=255)
                                                                                                                                                gACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTCATCTACGCCTGGTTCgag  >  2:9128776/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGCTATGCGCCGGGCGACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACCCTGCGTACGCCGCCGGAGCAACGCGACAAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTCATCTACGCCTGGTTCGAG  >  NC_008463/2407316‑2407609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑