| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_008463 | 4,659,831 | Δ1 bp | intergenic (+71/+28) | alpR → / ← PA14_RS21395 | transcriptional regulator AlpR/tRNA‑Arg |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_008463 | 4,659,828 | 0 | G | . | 95.0% | 1527.1 / 93.6 | 351 | intergenic (+68/+31) | alpR/PA14_RS21395 | transcriptional regulator AlpR/tRNA‑Arg |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base . (116/210); minor base A (8/9); total (132/219) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 382 total aligned reads displayed.
GGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAAAAAAATCCCGGGCAAAAAAAAAGGGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGTCGGTGCTTTTTCCGGCCACCTGCCGGTAACGCTTG > NC_008463/4659679‑4659974 | ggACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAAg < 2:3484287/150‑1 (MQ=255)ggACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAAg < 1:292227/150‑1 (MQ=255) gACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCTCATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAAgg > 2:5790453/1‑150 (MQ=255) cGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAAgggc < 2:6759829/150‑2 (MQ=255) gAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAAgggcc < 1:7155711/150‑3 (MQ=255) tgcgcTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAAgggcct > 1:5147673/2‑147 (MQ=255) gcgcTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAAgggcctg < 1:6791110/150‑5 (MQ=255) gcgcTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAAgggcctg < 2:198271/150‑5 (MQ=255) cgcTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGc < 2:5538818/150‑1 (MQ=255) gcTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCa < 1:1239904/150‑1 (MQ=255) cTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCAt > 1:721838/1‑150 (MQ=255) cTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCAt < 2:1451820/150‑1 (MQ=255) cTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCAt < 1:2686285/150‑1 (MQ=255) tACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCAtt < 1:7543200/150‑1 (MQ=255) tACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCAtt > 1:4126536/1‑150 (MQ=255) tACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCAtt < 1:1184592/150‑1 (MQ=255) aCGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATtg < 1:9816643/150‑1 (MQ=255) aCGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATtg > 1:3349529/1‑150 (MQ=255) gAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATtgct < 1:3211054/150‑1 (MQ=255) gAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCGTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATtgct < 2:2572760/150‑1 (MQ=255) aGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATtgctg > 2:4662520/1‑150 (MQ=255) aGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATtgctg < 2:8746119/150‑1 (MQ=255) ccAGGACGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTCCTCGGTGCTGGTCTGCCCCCGGCCCGCCCCGCCTGCCGACGGGGCCGCGTTCCTGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAgg < 2:9920714/150‑1 (MQ=255) ccAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCAGTGCTGCAgg > 2:2268715/1‑150 (MQ=255) ggAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGccc < 1:9016296/150‑1 (MQ=255) ggAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGccc < 1:8081425/150‑1 (MQ=255) gAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCt < 1:8324015/150‑1 (MQ=255) aGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAACA‑GGGCCTGCATTGCTGCAGGCCCttt < 2:8358950/150‑1 (MQ=255) ccgAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTa < 1:1055491/150‑1 (MQ=255) cgAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTAt > 2:9120950/1‑150 (MQ=255) gAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATg < 2:3363037/150‑1 (MQ=255) aGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATgg > 2:6886875/1‑150 (MQ=255) gCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTGCGGTATGgc > 1:4434868/1‑150 (MQ=255) cacaTCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGgcg > 1:8665644/1‑150 (MQ=255) acaTCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGgcgc < 1:8008287/150‑1 (MQ=255) caTCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCa < 2:9010061/150‑1 (MQ=255) aaCGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCa > 2:4104576/1‑150 (MQ=255) gTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCagga < 2:5001585/150‑1 (MQ=255) gATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCaggagg < 1:8824136/150‑1 (MQ=255) tCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGAt < 1:173540/150‑1 (MQ=255) tCGGCCGGGTGGTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGTGTCCGCTTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGAt < 1:10059903/150‑1 (MQ=255) gCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCg > 2:10475796/1‑150 (MQ=255) gggTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAAc > 1:10690666/1‑150 (MQ=255) gggTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAAc < 1:2242909/150‑1 (MQ=255) gggTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAAc > 2:7574343/1‑150 (MQ=255) gtgtTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCt > 1:2115376/1‑150 (MQ=255) tgtTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTc < 2:4849559/150‑1 (MQ=255) gtTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTcc < 1:2358271/150‑1 (MQ=255) gtTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTcc < 1:8368670/150‑1 (MQ=255) ttCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCg < 1:5220591/150‑1 (MQ=255) tCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGa > 2:4183111/1‑150 (MQ=255) aCTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGGTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTc < 2:1634001/150‑1 (MQ=255) cTCGGTGCTGGTCTGACCCCGACCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCg < 1:5380898/150‑1 (MQ=39) ggTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGtt > 2:3203417/1‑150 (MQ=255) gTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTc < 2:7388014/150‑1 (MQ=255) tGCTGGTCTGACCCCGTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCg < 1:4775072/150‑1 (MQ=255) tGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAg < 1:8036343/150‑1 (MQ=255) ggTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGc > 1:9249815/1‑150 (MQ=255) ggTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGC‑AAAGAAATTGGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGc > 2:9770607/1‑150 (MQ=38) tGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAg < 1:5631429/150‑1 (MQ=255) ccTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTActc < 2:7057969/150‑1 (MQ=255) ttCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTa < 1:2551523/150‑1 (MQ=255) ttCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACATCCGACCGCTCGGTTCGTAGCCGAGTACTCTa > 2:8497403/1‑150 (MQ=255) ctCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGACACA‑GGGCCGGCATTGCTGCAGGCCCTATCGGTATGGCGCACTCAGGAGGATTCGAACCGCCGACCGCTCGGTTCGTAGCCGAGTACTCTa < 2:10199556/149‑1 (MQ=25) cccgccccgcCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATc > 1:5577879/1‑150 (MQ=255) ccgccccgccTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATcc > 1:3870416/1‑150 (MQ=255) cccgccTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCt > 1:5933491/1‑150 (MQ=255) cgccTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGa < 1:7590392/150‑1 (MQ=255) ccTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGc < 1:1619659/150‑1 (MQ=255) cTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCt < 1:9629981/150‑1 (MQ=255) tGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTa < 1:780610/150‑1 (MQ=255) tGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTa > 2:5322518/1‑150 (MQ=255) gacgGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAg > 1:3131230/1‑150 (MQ=255) acgGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGt < 2:9237354/150‑1 (MQ=255) acgGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGt > 2:3866761/1‑150 (MQ=255) acgGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGt > 1:9491731/1‑150 (MQ=255) ggggCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGc > 1:10758117/1‑150 (MQ=255) ggggCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGc < 1:8445573/150‑1 (MQ=255) ccGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCgtgt > 2:4422935/1‑150 (MQ=255) cgcgTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGTc < 2:124824/150‑1 (MQ=255) cgcgTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGTc < 2:1111852/150‑1 (MQ=255) gcgTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGTCg < 2:6102223/150‑1 (MQ=255) gTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGTCGGt > 1:7322025/1‑150 (MQ=255) gTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGGATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGTCGGt > 1:7314742/1‑150 (MQ=255) ttCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGTCGGTg > 1:3542972/1‑150 (MQ=255) ttCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGTCGGTg > 1:6261386/1‑150 (MQ=255) tCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGTCGGTGc < 2:7668015/150‑1 (MQ=255) tCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGTCGGTGc > 1:6919525/1‑150 (MQ=255) tCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGTCGGTGc > 1:1524471/1‑150 (MQ=255) gcggAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGTCGGTGCtttt > 2:9516647/1‑150 (MQ=255) ggAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGTCGGTGCGTTTTc > 2:6466529/1‑150 (MQ=255) gaagaaAAATCCCGGGCAAAGAAACA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGTCGGTGCTTTTTcc < 2:8118211/150‑1 (MQ=255) aaaaaTCCCGGGCAAAGAAAAA‑GCGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGTCGGTGCTTTTTCCGGcc < 1:6523606/150‑1 (MQ=255) aaaaTCCCGGGCAAAGAAACA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCGATCCAGCTGAGCGATGAGTGCGTGTCGGTGCTTTTTCCGGCCa < 2:3002085/150‑1 (MQ=25) aaaaTCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCGTGCGGTGTGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGGTCGTAGCCGAGTACTCTAGCCAGCTGAGCGTTGAGTGCGTGTCGGTGCTTATTCCGGCCa > 2:627907/1‑150 (MQ=21) aaTCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGTCGGTGCTTTTTCCGGCCAcc < 1:6595441/150‑1 (MQ=255) gcaaagaaaaAGGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGTCGGTGCTTTTTCCGGCCACCTGCCGGTa < 1:4509671/148‑1 (MQ=255) caaagaaaaAGGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGTCGGTGCTTTTTCCGGCCACCTGCCGGTaa < 2:2109452/149‑1 (MQ=255) caaagaaaaAGGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGTCGGTGCTTTTTCCGGCCACCTGCCGGTaa < 1:10262050/149‑1 (MQ=255) gaaaaAGGGCCTGCAGTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTCGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGTCGGTGCTTTTTCCGGCCACCTGCCGGTCACGcg > 2:2728908/2‑149 (MQ=255) | GGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAAAAAAATCCCGGGCAAAAAAAAAGGGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGTCGGTGCTTTTTCCGGCCACCTGCCGGTAACGCTTG > NC_008463/4659679‑4659974 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |