BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	4,700,807	Δ1 bp	intergenic (+165/‑150)	PA14_RS21585 → / → pbpG	aspartate/tyrosine/aromatic aminotransferase/D‑alanyl‑D‑alanine endopeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	4,700,807	0	G	.	100.0%	2371.2 / NA	478	intergenic (+165/‑150)	PA14_RS21585/pbpG	aspartate/tyrosine/aromatic aminotransferase/D‑alanyl‑D‑alanine endopeptidase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (175/303); total (175/303)

Only 100 of 478 total aligned reads displayed.

GCCGGCTGTGATGGCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGCGCGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTTCTCGTCTTAGTGAGCAA  >  NC_008463/4700662‑4700951

gCCGGCTGTGATGGCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAAc                                                                                                                                             >  2:6392174/1‑150 (MQ=255)

gCCGGCTGTGATGGCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGGTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAAc                                                                                                                                             <  1:8239345/150‑1 (MQ=255)

 ccGGCTGTGATGGCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAAcc                                                                                                                                            <  2:7843530/150‑1 (MQ=255)

  cGGCTGTGATGGCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACct                                                                                                                                           <  2:6795622/150‑1 (MQ=255)

   ggCTGTGATGGCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACctc                                                                                                                                          <  1:6139267/150‑1 (MQ=255)

     cTGTGATGGCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCtctc                                                                                                                                        <  2:8566941/150‑1 (MQ=255)

      tgtgATGGCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTcc                                                                                                                                       <  2:1811671/150‑1 (MQ=255)

      tgtgATGGCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTcc                                                                                                                                       <  2:66195/150‑1 (MQ=255)

      tgtgATGGCCGGCTTTTGTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGGTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTcc                                                                                                                                       <  2:5554873/150‑1 (MQ=255)

       gtgATGGCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCg                                                                                                                                      <  1:4302958/150‑1 (MQ=255)

            ggCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCg                                                                                                                                 <  2:2023241/150‑1 (MQ=255)

             gCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGAGAACCGCGTTGCGGTTATTCGCCCGACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGt                                                                                                                                <  2:6354211/150‑1 (MQ=255)

              ccGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTg                                                                                                                               >  1:5020582/1‑150 (MQ=255)

               cGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGc                                                                                                                              <  2:3460333/150‑1 (MQ=255)

               cGGCATTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGc                                                                                                                              <  2:3868417/150‑1 (MQ=255)

                ggCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGcc                                                                                                                             >  1:3465266/1‑150 (MQ=255)

                     tttttCGCCCCAGCGAATGCCGCGCACGCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCa                                                                                                                        >  2:2983362/1‑150 (MQ=255)

                       tttCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATa                                                                                                                      <  1:4115234/150‑1 (MQ=255)

                        ttCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATaa                                                                                                                     <  1:4913277/150‑1 (MQ=255)

                          cGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGt                                                                                                                   <  1:10771424/150‑1 (MQ=255)

                            ccccAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCAGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCa                                                                                                                 <  2:10762439/150‑1 (MQ=255)

                              ccAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGc                                                                                                               >  1:589858/1‑150 (MQ=255)

                               cAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCa                                                                                                              >  1:1434901/1‑150 (MQ=255)

                               cAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCa                                                                                                              <  2:9068835/150‑1 (MQ=255)

                                aGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCAc                                                                                                             <  2:7119505/150‑1 (MQ=255)

                                  cGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACtt                                                                                                           <  1:972614/150‑1 (MQ=255)

                                   gAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGCACCTCTCCGAACCGTGCCACCCATAAGTCAGCTCttt                                                                                                          >  2:3622069/1‑150 (MQ=255)

                                   gAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACttt                                                                                                          <  1:2151278/150‑1 (MQ=255)

                                    aaTGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTc                                                                                                         <  1:6482378/150‑1 (MQ=255)

                                     aTGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCa                                                                                                        >  1:2952374/1‑150 (MQ=255)

                                         cgcgCACTCCCGCCGAGGCGGCGGATCACCGCGTTGCGGTTATTCGCCCTACCGCACCACCCCCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGGCAGCACTTTCAAAGc                                                                                                    <  2:6130213/150‑1 (MQ=255)

                                          gcgcACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGcc                                                                                                   <  2:1571604/150‑1 (MQ=255)

                                           cgcACTCCCGCCGAGGCGGCGGCTAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAACGccc                                                                                                  <  2:3180133/150‑1 (MQ=255)

                                             cACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTa                                                                                                <  2:2706227/150‑1 (MQ=255)

                                             cACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTa                                                                                                >  2:8043016/1‑150 (MQ=255)

                                               cTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACg                                                                                              <  1:1984869/150‑1 (MQ=255)

                                                 cccGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCCTAAGTCAGCACTTTCAAAGCCCTACGTg                                                                                            <  2:8266205/150‑1 (MQ=255)

                                                   cgccgAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCt                                                                                          <  2:10365299/150‑1 (MQ=255)

                                                    gccgAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGACTACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCtt                                                                                         <  2:9578247/150‑1 (MQ=255)

                                                    gccgAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCtt                                                                                         <  1:3256712/150‑1 (MQ=255)

                                                    gccgAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCtt                                                                                         <  2:4486394/150‑1 (MQ=255)

                                                    gccgAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCtt                                                                                         <  1:5295725/150‑1 (MQ=255)

                                                      cgAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCtttt                                                                                       <  1:2635457/150‑1 (MQ=255)

                                                      cgAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCattt                                                                                       <  1:3656786/150‑1 (MQ=255)

                                                       gAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCAGGACCTTGTGGGCGTTGGGCGAATACCGCCCCAGGCGATATCCGC‑CGAACCTCTCCGAACCGGGCCAACCATTAGTCAGCACTGTCTATGGCCGACGTGCttttg                                                                                      >  2:5857053/1‑149 (MQ=255)

                                                        aGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTcc                                                                                     >  2:2554497/1‑150 (MQ=255)

                                                        aGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGCGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTcc                                                                                     <  1:10184420/150‑1 (MQ=255)

                                                          gcggcgGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTa                                                                                   >  1:9404595/1‑150 (MQ=255)

                                                          gcggcgGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTa                                                                                   <  2:2261217/150‑1 (MQ=255)

                                                           cggcggATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTAc                                                                                  <  2:8219584/150‑1 (MQ=255)

                                                            ggcggATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACa                                                                                 >  2:8751681/1‑150 (MQ=255)

                                                             gcggATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACaa                                                                                <  1:3310201/150‑1 (MQ=255)

                                                                 aTAACCGCGGTGCGGTTATTCGCCCTACCGCACCCCGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAaga                                                                            <  1:7703736/150‑1 (MQ=255)

                                                                   aaCCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGNGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTGTCCTACAAAAGAGc                                                                          >  1:5676435/1‑150 (MQ=255)

                                                                   aaCCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGc                                                                          <  2:5004735/150‑1 (MQ=255)

                                                                       gcgTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATaa                                                                      <  1:2316871/150‑1 (MQ=255)

                                                                        cgTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATaaa                                                                     <  2:1292184/150‑1 (MQ=255)

                                                                          ttGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAAt                                                                   <  2:9418128/150‑1 (MQ=255)

                                                                             cGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCt                                                                <  2:4644393/150‑1 (MQ=255)

                                                                             cGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCt                                                                <  1:8835611/150‑1 (MQ=255)

                                                                              ggTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTc                                                               <  2:4852064/150‑1 (MQ=255)

                                                                              ggTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTc                                                               <  2:234011/150‑1 (MQ=255)

                                                                              ggTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTc                                                               <  1:6747302/150‑1 (MQ=255)

                                                                               gTTATTCGCCCTACCGCACCACGAACTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAAACGTGCCTACCATAAGTCAGCACTTTCAAAGCCCTCCGTGCTTTTCCTACAAAAGAGCATAAAATGCTCa                                                              <  1:4474169/150‑1 (MQ=255)

                                                                                 tATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAAt                                                            >  1:5984560/1‑150 (MQ=255)

                                                                                   ttCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTa                                                          >  2:4136161/1‑150 (MQ=255)

                                                                                    tCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTAt                                                         >  1:10046803/1‑150 (MQ=255)

                                                                                     cGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATg                                                        <  2:1026637/150‑1 (MQ=255)

                                                                                     cGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATg                                                        <  1:8706830/150‑1 (MQ=255)

                                                                                     cGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATg                                                        <  1:1160129/150‑1 (MQ=255)

                                                                                      gCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATgg                                                       >  1:1838495/1‑150 (MQ=255)

                                                                                      gCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATgg                                                       <  2:10636239/150‑1 (MQ=255)

                                                                                       cccTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATggg                                                      <  2:7507568/150‑1 (MQ=255)

                                                                                         cTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCa                                                    >  2:9783655/1‑150 (MQ=255)

                                                                                             cGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAg                                                <  1:10444669/150‑1 (MQ=255)

                                                                                                  cacGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTcac                                           <  2:8469091/150‑1 (MQ=255)

                                                                                                   acGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTcaca                                          >  2:1184778/1‑150 (MQ=255)

                                                                                                   acGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTcaca                                          <  1:6774447/150‑1 (MQ=255)

                                                                                                   acGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTcaca                                          >  1:7032806/1‑150 (MQ=255)

                                                                                                      aCCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTg                                       <  2:9190625/150‑1 (MQ=255)

                                                                                                            tgGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGcccc                                 <  2:5323442/150‑1 (MQ=255)

                                                                                                             gggCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCa                                <  1:7294758/150‑1 (MQ=255)

                                                                                                             gggCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCa                                >  1:6862148/1‑150 (MQ=255)

                                                                                                               gCGTAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAACGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACg                              <  1:5418983/150‑1 (MQ=255)

                                                                                                                  tAGGGCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGcc                           >  1:10621313/1‑150 (MQ=255)

                                                                                                                    gggCGAATACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGcccc                         >  1:8296234/1‑150 (MQ=255)

                                                                                                                         aaTACCGCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACtt                    >  2:4012308/1‑150 (MQ=255)

                                                                                                                               gCCACAGGCGTTATCCGC‑CGAACCTCTCCGACCCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAACTGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTTCTCGt              <  2:7403546/150‑1 (MQ=255)

                                                                                                                               gCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATACGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACACAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTTCTCGt              <  1:3891432/150‑1 (MQ=255)

                                                                                                                               gCCACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTTCTCGt              <  1:9605508/150‑1 (MQ=255)

                                                                                                                                ccACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTTCTCGTc             <  1:7424602/150‑1 (MQ=255)

                                                                                                                                ccACAGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTTCTCGTc             >  1:8556683/1‑150 (MQ=255)

                                                                                                                                  acaGGCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTTCTCGTCtt           >  2:4339386/1‑150 (MQ=255)

                                                                                                                                     ggCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTTCTCGTCTTAGt        <  1:7589053/150‑1 (MQ=255)

                                                                                                                                     ggCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTTCTCGTCTTAGt        >  2:10029455/1‑150 (MQ=255)

                                                                                                                                      gCGTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTTCTCGTCTTAGTg       <  2:5956938/150‑1 (MQ=255)

                                                                                                                                        gTTATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAACATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTTCTCGTCTTAGTGAg     <  2:2899470/150‑1 (MQ=255)

                                                                                                                                         ttATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACCAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTTCTCGTCTTAGTGAGc    <  1:7215357/150‑1 (MQ=255)

                                                                                                                                         ttATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTTCTCGTCTTAGTGAGc    <  1:4816332/150‑1 (MQ=255)

                                                                                                                                          tATCCGC‑CGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTTCTCGTCTTAGTGAGCa   <  1:9127314/150‑1 (MQ=255)

GCCGGCTGTGATGGCCGGCTTTTTTTCGCCCCAGCGAATGCCGCGCACTCCCGCCGAGGCGGCGGATAACCGCGTTGCGGTTATTCGCCCTACCGCACCACGACCTTGTGGGCGTAGGGCGAATACCGCCACAGGCGTTATCCGCGCGAACCTCTCCGAACCGTGCCAACCATAAGTCAGCACTTTCAAAGCCCTACGTGCTTTTCCTACAAAAGAGCATAAAATGCTCAATTATGGGCACCAGTTCACACTGCGCCCCACGGCCCCCACTTTCTCGTCTTAGTGAGCAA  >  NC_008463/4700662‑4700951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`