BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	6,070,106	Δ1 bp	pseudogene (575/1086 nt)	PA14_RS27735 →	oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	6,070,105	0	A	.	99.8%	2866.7 / ‑3.5	579	pseudogene (574/1086 nt)	PA14_RS27735	oxidoreductase
Reads supporting (aligned to +/- strand): ref base A (0/1); new base . (280/298); total (280/299)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 595 total aligned reads displayed.

GGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGCTTCCGGGCTGTGGTTTGACCTTGGCCCGCAACCTGGTTGACCAAGGCACTGTGCCTGTTTGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTGCGCGCCAGCATGCT  >  NC_008463/6069954‑6070244

 gccgccCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACCag                                                                                                                                            >  1:7653163/1‑149 (MQ=255)

 gccgccCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACCag                                                                                                                                            <  2:8230121/150‑2 (MQ=255)

  ccgccCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACCagg                                                                                                                                           >  1:4357952/1‑148 (MQ=255)

     cccTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCAc                                                                                                                                       >  1:360070/1‑150 (MQ=255)

     cccTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCAc                                                                                                                                       >  1:1688980/1‑150 (MQ=255)

      ccTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACt                                                                                                                                      <  1:209292/150‑1 (MQ=255)

       cTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGTCCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACtg                                                                                                                                     >  2:7247197/1‑150 (MQ=255)

            ggCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCt                                                                                                                                <  1:4400518/150‑1 (MQ=255)

             gCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTg                                                                                                                               <  1:1088146/150‑1 (MQ=255)

              ccGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGt                                                                                                                              <  1:7839925/150‑1 (MQ=255)

                 gcagcaTCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGttcg                                                                                                                           >  2:1689608/1‑148 (MQ=255)

                 gcagcaTCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGttcg                                                                                                                           >  1:4164525/1‑148 (MQ=255)

                  cagcaTCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCgg                                                                                                                          >  1:1750473/1‑150 (MQ=255)

                   agcaTCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGc                                                                                                                         >  1:9730910/1‑150 (MQ=255)

                    gcaTCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCa                                                                                                                        >  1:9304001/1‑150 (MQ=255)

                     caTCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCAt                                                                                                                       <  1:7510728/150‑1 (MQ=255)

                          gCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGc                                                                                                                  >  1:5838089/1‑150 (MQ=255)

                           cccGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCa                                                                                                                 >  1:8246721/1‑150 (MQ=255)

                           cccGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCa                                                                                                                 <  2:4976959/150‑1 (MQ=255)

                             cGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGc                                                                                                               >  1:4942311/1‑150 (MQ=255)

                               gTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGt                                                                                                             <  2:2963853/150‑1 (MQ=255)

                               gTGCGCTACTTCGAGTCGGCCATCGAGCGCGTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGt                                                                                                             <  2:6118398/150‑1 (MQ=255)

                                tGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTa                                                                                                            <  1:2807914/150‑1 (MQ=255)

                                tGCGCTACTTCGAGTCGGCCATCGAGCGCGTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTa                                                                                                            <  2:6065577/150‑1 (MQ=255)

                                  cgcTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATg                                                                                                          >  2:5278191/1‑150 (MQ=255)

                                  cgcTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATg                                                                                                          <  2:3029706/150‑1 (MQ=255)

                                   gcTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGc                                                                                                         <  2:1077467/150‑1 (MQ=255)

                                      aCTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACg                                                                                                      >  1:10383197/1‑150 (MQ=255)

                                      aCTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACg                                                                                                      <  2:852233/150‑1 (MQ=255)

                                       cTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACgg                                                                                                     >  2:1849889/1‑150 (MQ=255)

                                         tCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGac                                                                                                   <  2:7735322/150‑1 (MQ=255)

                                          cGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGaca                                                                                                  >  2:9285784/1‑150 (MQ=255)

                                          agAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGAATGCACGGaca                                                                                                  >  2:3889525/2‑150 (MQ=255)

                                            aGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACTcc                                                                                                <  2:4172374/150‑1 (MQ=255)

                                            aGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACAcc                                                                                                <  2:9562950/150‑1 (MQ=255)

                                               cGGCCCTCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGCACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGTCCTCGGCCCGC‑ACCTGCTCGACC‑CGGCACTGTGCCTGTTCGGCATAGCGCAGCGTATGCACGGACACCTgc                                                                                             >  2:6526638/1‑150 (MQ=255)

                                               cGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTgc                                                                                             >  1:10652414/1‑150 (MQ=255)

                                                 gCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTgcgc                                                                                           >  1:8110509/1‑150 (MQ=255)

                                                 gCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTgcgc                                                                                           >  1:10010051/1‑150 (MQ=255)

                                                  ccATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGcc                                                                                          <  2:6706457/150‑1 (MQ=255)

                                                   cATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCg                                                                                         >  1:4678785/1‑150 (MQ=255)

                                                    aTCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCgg                                                                                        >  1:2467878/1‑150 (MQ=255)

                                                     tCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGc                                                                                       >  1:7272442/1‑150 (MQ=255)

                                                      cGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCt                                                                                      <  1:9169417/150‑1 (MQ=255)

                                                      cGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCt                                                                                      >  2:9747775/1‑150 (MQ=255)

                                                         gcgcTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTgcg                                                                                   <  1:3072109/150‑1 (MQ=255)

                                                          cgcTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTgcgc                                                                                  >  1:2213846/1‑150 (MQ=255)

                                                            cTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGa                                                                                >  1:10544328/1‑150 (MQ=255)

                                                             ttCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAg                                                                               <  2:5529784/150‑1 (MQ=255)

                                                                cgtccgCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGgc                                                                            <  1:3844842/150‑1 (MQ=255)

                                                                    cgCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGgcgcgc                                                                        >  2:2554960/1‑150 (MQ=255)

                                                                    cgCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGgcgcgc                                                                        >  2:7947296/1‑150 (MQ=255)

                                                                     gCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCt                                                                       <  1:238855/150‑1 (MQ=255)

                                                                      cAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACTGACACCTGCGCCGGCTGCGCGAGGGCGCGCTg                                                                      >  2:4027344/1‑150 (MQ=255)

                                                                       aGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGa                                                                     >  2:3358649/1‑150 (MQ=255)

                                                                        ggTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGAc                                                                    <  1:9044705/150‑1 (MQ=255)

                                                                               gACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCgacgac                                                             <  2:28774/150‑1 (MQ=255)

                                                                                 ccGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTg                                                           >  2:4762724/1‑150 (MQ=255)

                                                                                  cGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACggg                                                          >  2:2236253/1‑150 (MQ=255)

                                                                                   gCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGt                                                         <  1:7386520/150‑1 (MQ=255)

                                                                                     tGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTc                                                       >  2:5137521/1‑150 (MQ=255)

                                                                                     tGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTc                                                       >  1:7079194/1‑150 (MQ=255)

                                                                                     tGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTc                                                       <  2:750313/150‑1 (MQ=255)

                                                                                      ggCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCg                                                      <  1:9616074/150‑1 (MQ=255)

                                                                                      ggCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCg                                                      <  1:700230/150‑1 (MQ=255)

                                                                                         gcgAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAgg                                                   <  1:2697474/150‑1 (MQ=255)

                                                                                         gcgAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAgg                                                   <  2:10191041/150‑1 (MQ=255)

                                                                                         gcgAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAgg                                                   >  2:1417059/1‑150 (MQ=255)

                                                                                            aaCACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTcc                                                <  2:5773390/150‑1 (MQ=255)

                                                                                             acacGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCt                                               <  2:7378850/150‑1 (MQ=255)

                                                                                             acacGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCt                                               <  2:4659011/150‑1 (MQ=255)

                                                                                             acacGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCt                                               <  1:6715123/150‑1 (MQ=255)

                                                                                                cGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCt                                            >  1:8685828/1‑150 (MQ=255)

                                                                                                cGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCt                                            >  2:9108405/1‑150 (MQ=255)

                                                                                                   ccTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGa                                         >  1:5263580/1‑150 (MQ=255)

                                                                                                    cTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGAc                                        <  1:6028804/150‑1 (MQ=255)

                                                                                                      gCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTa                                      >  1:9898527/1‑150 (MQ=255)

                                                                                                       cccGGC‑TCCGGGCTGTGTTTCGACCTCGGCCAGC‑ACCTGCTCGACC‑AGGCAATGTGCCTGTTCGGCATACCGCAGCGTAAGCACGGACACCTGCTCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTAt                                     >  1:6259547/1‑150 (MQ=255)

                                                                                                          cggctCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATcc                                   <  1:3304845/146‑1 (MQ=255)

                                                                                                          cggctCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATcc                                   >  2:8345451/5‑150 (MQ=255)

                                                                                                             ctCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCa                                >  1:5062579/2‑150 (MQ=255)

                                                                                                                     tgtgGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAgg                        <  1:1344703/150‑1 (MQ=255)

                                                                                                                      gtgGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGt                       >  1:3984593/1‑150 (MQ=255)

                                                                                                                      gtgGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGt                       <  2:2683594/150‑1 (MQ=255)

                                                                                                                         gTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGgc                    <  1:5492328/150‑1 (MQ=255)

                                                                                                                          ttcgACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGCGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGgcg                   <  2:10081451/147‑1 (MQ=255)

                                                                                                                              aCCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCTCGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTgc               <  2:1316497/150‑1 (MQ=255)

                                                                                                                              aCCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTgc               <  1:3499832/150‑1 (MQ=255)

                                                                                                                               ccTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTgcg              <  1:6552370/150‑1 (MQ=255)

                                                                                                                                ctcggCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTgcgc             <  2:3165215/147‑1 (MQ=255)

                                                                                                                                ctcggCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTgcgc             <  1:6428215/147‑1 (MQ=255)

                                                                                                                                 tcggCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCCGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACGGGTTCGCGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTgcgcg            >  2:10717519/3‑150 (MQ=255)

                                                                                                                                 tcggCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTgcgcg            >  1:1174513/3‑150 (MQ=255)

                                                                                                                                 tcggCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTgcgcg            >  1:8966028/3‑150 (MQ=255)

                                                                                                                                   ggCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTGCGCGcc          <  2:4336548/150‑1 (MQ=255)

                                                                                                                                   ggCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTGCGCGcc          <  2:10630823/150‑1 (MQ=255)

                                                                                                                                    gCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTGCGCGCCa         >  1:5616734/1‑150 (MQ=255)

                                                                                                                                        cgcaCCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTGCGCGCCAGCa      >  2:8816652/4‑150 (MQ=255)

                                                                                                                                         gcaCCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTGCGCGCCAGCAt     >  2:702029/3‑150 (MQ=255)

                                                                                                                                           aCCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTGCGCGCCAGCATGc   <  2:6434846/150‑1 (MQ=255)

GGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGCTTCCGGGCTGTGGTTTGACCTTGGCCCGCAACCTGGTTGACCAAGGCACTGTGCCTGTTTGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCCGCACTGCCAGGTGGCGCTGCGCGCCAGCATGCT  >  NC_008463/6069954‑6070244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`