Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084635,116,3910CT31.8% 708.3 / inf 574R504H (CGC→CAC) ftsIpeptidoglycan D,D‑transpeptidase FtsI
Reads supporting (aligned to +/- strand):  ref base C (190/200);  new base T (94/88);  total (285/289)
Fisher's exact test for biased strand distribution p-value = 5.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 623 total aligned reads displayed.

TCATCAGGCGCAGCGCGCCAGCCATGACCTTGCTGAACACCGGCGCCGACACCAGGCCGCCGAAGTAGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTGGAA  >  NC_008463/5116242‑5116540
                                                                                                                                                     |                                                                                                                                                     
 catcaGGCGCAGCGCGCCAGCCATGACCTTGCTGAACACCGGCGCCGACACCAGGCCGCCGAAGTAGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACagcgagcg                                                                                                                                                      <  2:123130/150‑1 (MQ=255)
   tcaGGCGCAGCGCGCCAGCCATGACCTTGCTGAACACCGGCGCCGACACCAGGCCGCCGAAGTAGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGAt                                                                                                                                                    >  2:4895293/1‑150 (MQ=255)
    caGGCGCAGCGCGCCAGCCATGACCTTGCTGAACACCGGCGCCGACACCAGGCCGCCGAAGTAGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGTGATa                                                                                                                                                   >  2:7383755/1‑150 (MQ=255)
    caGGCGCAGCGCGCCAGCCATGACCTTGCTGAACACCGGCGCCGACACCAGGCCGCCGAAGTAGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGTGATa                                                                                                                                                   >  2:2359106/1‑150 (MQ=255)
          cAGCGCGCCAGCCATGACCTTGCTGAACACCGGCGCCGACACCAGGCCGCCGAAGTAGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGTGATAGGCGtt                                                                                                                                             >  2:10245432/1‑150 (MQ=255)
           aGCGCGCCAGCCATGACCTTGCAGAACACCGGCGCCGACACCAGGCCGCCGAAGTAGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGttt                                                                                                                                            <  2:9212178/150‑1 (MQ=255)
            gcgcgcCAGCCATGACCTTGCTGAACACCGGCGCCGACACCAGGCCGCCGAAGTAGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGtttt                                                                                                                                           >  1:4797399/1‑150 (MQ=255)
              gcgcCAGCCATGACCTTGCTGAACACCGGCGCCGACACCAGGCCGCCGAAGTAGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGTGATAGGCGTTTTcc                                                                                                                                         >  2:387179/1‑150 (MQ=255)
               cgcCAGCCATGACCTTGCTGAACACCGGCGCCGACACCAGGCCGCCGAAGTAGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGTGATAGGCGTTTTccc                                                                                                                                        <  1:2360758/150‑1 (MQ=255)
                 ccagccaTGACCTTGCTGAACACCGGCGCCGACACCAGGCCGCCGAAGTAGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCgg                                                                                                                                      >  1:1936799/1‑150 (MQ=255)
                  cagccaTGACCTTGCTGAACACCGGCGCCGCCACCAGGCCGCCGAAGTAGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGt                                                                                                                                     <  1:8340354/150‑1 (MQ=255)
                   agccaTGACCTTGCTGAACACCGGCGCCGACACCAGTCCGCCGAAGTAGCCCGCCTGGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGTGATAGGCGTTTTCCCGGTa                                                                                                                                    <  2:80621/150‑1 (MQ=255)
                   agccaTGACCTTGCTGAACACCGGCGCCGACACCAGGCCGCCGAAGTAGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGTGATAGGCGTTTTCCCGGTa                                                                                                                                    >  1:6909490/1‑150 (MQ=255)
                    gccaTGACCTTGCTGAACACCGGCGCCGACACCAGGCCGCCGAAGTAGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAg                                                                                                                                   >  2:1766741/1‑150 (MQ=255)
                     ccaTGACCTTGCTGAACACCGGCGCCGACACCAGGCCGCCGAAGTAGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGc                                                                                                                                  <  1:10302247/150‑1 (MQ=255)
                     ccaTGACCTTGCTGAACACCGGCGCCGACACCAGGCCGCCGAAGTAGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGc                                                                                                                                  <  2:10633827/150‑1 (MQ=255)
                       aTGACCTTGCTGAACACCGGCGCCGACACCAGGCCGCCGAAGTAGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGccc                                                                                                                                >  1:5233856/1‑150 (MQ=255)
                         gACCTTGCTGAACACCGGCGCCGACACCAGGCCGCCGAAGTAGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGTGATAGGCGTTTTCCCGGTAGCCCtt                                                                                                                              <  1:10022992/150‑1 (MQ=255)
                          aCCTTGCTGAACACCGGCGCCGACACCAGGCCGCCGAAGTAGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTg                                                                                                                             >  1:8520697/1‑150 (MQ=255)
                           ccTTGCTGAACACCGGCGCCGACACCAGGCCGCCGAAGTAGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTgg                                                                                                                            <  2:4602912/150‑1 (MQ=255)
                             ttGCTGAACACCGGCGCCGACACCAGGCCGCCGAAGTAGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCATCGGGATGCGCGGATCGGTGGCCGGGGCGAAACGGGCGAACAGCGAGTGATTGGCGTTTTCCCGGTAGCCCTTGGTg                                                                                                                          >  1:9080224/1‑150 (MQ=255)
                              tGCTGAACACCGGCGCCGACACCAGGCCGCCGAAGTAGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGTGATAGGCGTTTTCCCGGTAGCCCTTGGTGc                                                                                                                         <  1:3133318/150‑1 (MQ=255)
                              tGCTGAACACCGGCGCCGACACCAGGCCGCCGAAGTAGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGc                                                                                                                         <  1:4135276/150‑1 (MQ=255)
                              tGCTGAACACCGGCGCCGACACCAGGCCGCCGAAGTAGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGc                                                                                                                         <  2:2627924/150‑1 (MQ=255)
                               gCTGAACACCGGCGCCGACACCAGGCCGCCGAAGTAGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGcc                                                                                                                        <  1:2635073/150‑1 (MQ=255)
                                 tGAACACCGGCGCCGACACCAGGCCGCCGAAGTAGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGccga                                                                                                                      <  2:581290/150‑1 (MQ=255)
                                   aaCACCGGCGCCGACACCAGGCCGCCGAAGTAGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGccgacc                                                                                                                    >  2:7160139/1‑150 (MQ=255)
                                     cacCGGCGCCGACACCAGGCCGCCGAAGTAGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGccgaccga                                                                                                                  >  1:5994944/1‑150 (MQ=255)
                                        cGGCGCCGACACCAGGCCGCCGAAGTAGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGAc                                                                                                               <  1:9901757/150‑1 (MQ=255)
                                         ggCGCCGACACCCGGCCGCCGAAGTAGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGAcc                                                                                                              <  2:6374703/150‑1 (MQ=255)
                                         ggCGCCGACACCAGGCCGCCGAAGTAGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGTGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGAcc                                                                                                              >  2:10108110/1‑150 (MQ=255)
                                          gcgcCGACACCAGGCCGCCGAAGTAGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGTGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCt                                                                                                             >  1:3632005/1‑150 (MQ=255)
                                            gcCGACACCAGGCCGCCGAAGTAGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTg                                                                                                           >  1:5330261/1‑150 (MQ=255)
                                             ccGACACCAGGCCGCCGAAGTAGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGTGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTgc                                                                                                          >  1:4318559/1‑150 (MQ=255)
                                               gACACCAGGCCGCCGAAGTAGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTgcgc                                                                                                        >  2:2206360/1‑150 (MQ=255)
                                                acacCAGGCCGCCGAAGTAGCCCGCCTTGCTCTGCTCGTCGAACACCACGACAATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGTCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTgcgcg                                                                                                       <  1:7057096/150‑1 (MQ=255)
                                                acacCAGGCCGCCGAAGTAGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGACCAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTgcgcg                                                                                                       >  2:1381228/1‑150 (MQ=255)
                                                acacCAGGCCGCCGAAGTAGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGTGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTgcgcg                                                                                                       >  2:8761064/1‑150 (MQ=255)
                                                acacCAGGCCGCCGAAGTAGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGTGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTgcgcg                                                                                                       >  1:2222772/1‑150 (MQ=255)
                                                acacCAGGCCGCCGAAGTAGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTgcgcg                                                                                                       >  1:5056055/1‑150 (MQ=255)
                                                 cacCAGGCCGCCGAAGTAGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGTGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTgcgcgc                                                                                                      <  2:8980640/150‑1 (MQ=255)
                                                 cacCAGGCCGCCGAAGTAGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTgcgcgc                                                                                                      >  1:10329536/1‑150 (MQ=255)
                                                  acCAGGCCGCCGAAGTAGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCCTCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGcgcgcg                                                                                                     <  2:5481077/150‑1 (MQ=255)
                                                  acCAGGCCGCCGAAGTAGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGTGATAGGCGTTGTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGcgcgcg                                                                                                     >  2:3731416/1‑150 (MQ=255)
                                                  acCAGGCCGCCGAAGTAGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGcgcgcg                                                                                                     <  2:4450528/150‑1 (MQ=255)
                                                   ccAGGCCGCCGAAGTAGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCgg                                                                                                    >  2:2793678/1‑150 (MQ=255)
                                                     aGGCCGCCGAAGTAGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGTGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTc                                                                                                  >  2:9502370/1‑150 (MQ=255)
                                                     aGGCCGCCGAAGTAGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGTGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTc                                                                                                  >  1:7729429/1‑150 (MQ=255)
                                                            cgAAGTAGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGctct                                                                                           >  2:2686485/1‑150 (MQ=255)
                                                             gAAGTAGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGTGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCtt                                                                                          >  2:4233503/1‑150 (MQ=255)
                                                               aGTAGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGc                                                                                        >  1:9530383/1‑150 (MQ=255)
                                                                gTAGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGcc                                                                                       >  2:8571919/1‑150 (MQ=255)
                                                                  aGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCcgg                                                                                     <  1:3315707/150‑1 (MQ=255)
                                                                    cccGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGTGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCcggcg                                                                                   >  2:5316440/1‑150 (MQ=255)
                                                                        ccTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGTGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGt                                                                               <  2:3632005/150‑1 (MQ=255)
                                                                         cTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTg                                                                              >  1:8260959/1‑150 (MQ=255)
                                                                          ttGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGTGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTgg                                                                             >  2:2079410/1‑150 (MQ=255)
                                                                              tcggctcgTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGTGTTAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTTAc                                                                         <  1:9730791/150‑1 (MQ=255)
                                                                               cggctcgTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGTGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAAcc                                                                        <  1:799859/150‑1 (MQ=255)
                                                                                ggctcgTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGGAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAAccc                                                                       <  2:4017244/150‑1 (MQ=255)
                                                                                  ctcgTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGTGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCgg                                                                     <  2:6021484/150‑1 (MQ=255)
                                                                                   tcgtcgATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCGGc                                                                    <  1:6795469/150‑1 (MQ=255)
                                                                                       cgATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCGGCACCt                                                                <  1:4643394/150‑1 (MQ=255)
                                                                                        gATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCGGCACCTg                                                               >  1:4032585/1‑150 (MQ=255)
                                                                                        gATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCGGCACCTg                                                               >  1:9431628/1‑150 (MQ=255)
                                                                                         aTCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCGGCACCTgg                                                              >  2:2924155/1‑150 (MQ=255)
                                                                                         aTCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCGGCACCTgg                                                              <  2:322878/150‑1 (MQ=255)
                                                                                           caccacGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCGGCACCTGGgc                                                            >  1:5430507/1‑150 (MQ=255)
                                                                                              cacGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCGGCACCTGGGcgcg                                                         <  1:2560339/150‑1 (MQ=255)
                                                                                              cacGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCGGCACCTGGGcgcg                                                         <  1:127830/150‑1 (MQ=255)
                                                                                                cGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCGGCACCTGGGCGCGGa                                                       >  1:7583884/1‑150 (MQ=255)
                                                                                                cGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCGGCACCTGGGCGCGGa                                                       <  1:2073512/150‑1 (MQ=255)
                                                                                                 gACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCGGCACCTGGGCGCGGaa                                                      >  2:9440522/1‑150 (MQ=255)
                                                                                                   ccATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGTGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCGGCACCTGGGCGCGGAaca                                                    >  2:3458387/1‑150 (MQ=255)
                                                                                                     aTCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCGGCACCTGGGCGCGGAACAcc                                                  <  2:10499720/150‑1 (MQ=255)
                                                                                                      tCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCGGCACCTGGGCGCGGAACAccc                                                 >  2:7641174/1‑150 (MQ=255)
                                                                                                       cgcgATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCGGCACCTGGGCGCGGAACAcccc                                                <  2:6847395/150‑1 (MQ=255)
                                                                                                        gcgATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCg                                               >  1:3494824/1‑150 (MQ=255)
                                                                                                           aTGCGCGGATCGGTGGCCGGGGAGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGccc                                            <  1:1052588/150‑1 (MQ=255)
                                                                                                            tGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCt                                           >  2:202363/1‑150 (MQ=255)
                                                                                                              cgcgGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGTGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTgg                                         >  1:1692324/1‑150 (MQ=255)
                                                                                                               gcgGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTggg                                        <  1:6229101/150‑1 (MQ=255)
                                                                                                                 ggATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGcc                                      <  1:8646999/150‑1 (MQ=255)
                                                                                                                       gTGGCCGGGGCGAAACCGGCGAACAGCGAGTGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGAcc                                >  2:7944217/1‑150 (MQ=255)
                                                                                                                       gTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGAcc                                >  2:6623935/1‑150 (MQ=255)
                                                                                                                             ggggCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACttgt                          <  1:4709279/150‑1 (MQ=255)
                                                                                                                             ggggCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACttgt                          <  1:10761591/150‑1 (MQ=255)
                                                                                                                             ggggCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACttgt                          <  1:4990550/150‑1 (MQ=255)
                                                                                                                             ggggCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACttgt                          >  1:2741957/1‑150 (MQ=255)
                                                                                                                                  gAAACCGGCGAACAGCGAGTGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTgcag                     <  2:9924507/150‑1 (MQ=255)
                                                                                                                                      ccGGCGAACAGCGAGTGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATg                 <  2:19371/150‑1 (MQ=255)
                                                                                                                                       cGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGc                >  1:7358122/1‑150 (MQ=255)
                                                                                                                                           gAACAGCGAGTGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTg            >  1:6474884/1‑150 (MQ=255)
                                                                                                                                           gAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTg            <  2:5175533/150‑1 (MQ=255)
                                                                                                                                            aaCAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGc           <  2:1591333/150‑1 (MQ=255)
                                                                                                                                                gcgagcgaTAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACgg       >  1:5518142/1‑150 (MQ=255)
                                                                                                                                                gcgagcgaTAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACgg       <  2:5069554/150‑1 (MQ=255)
                                                                                                                                                  gagcgaTAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTg     >  2:6684248/1‑150 (MQ=255)
                                                                                                                                                  gagcgaTAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTg     >  2:4633597/1‑150 (MQ=255)
                                                                                                                                                    gcgaTAGGCGTTTTCCCGGTAGCCCGTGGTGCCGACCGAGACCTGGCGCGCGGTCCCGCTCGTGCCGGCGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTGGa   <  2:8294158/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCATCAGGCGCAGCGCGCCAGCCATGACCTTGCTGAACACCGGCGCCGACACCAGGCCGCCGAAGTAGCCCGCCTTGCTCGGCTCGTCGATCACCACGACCATCGCGATGCGCGGATCGGTGGCCGGGGCGAAACCGGCGAACAGCGAGCGATAGGCGTTTTCCCGGTAGCCCTTGGTGCCGACCGAGACCTTGCGCGCGGTCCCGCTCTTGCCGGCGGCGTGGTAACCCGGCACCTGGGCGCGGAACACCCCGCCCTGGGCCTCGACCACTTGTTGCAGCATGCCCTGCACGGTGGAA  >  NC_008463/5116242‑5116540

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑