Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,807,266 G→C V137L (GTG→CTG)  PA14_RS13175 → leucine‑rich repeat‑containing protein kinase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,807,2660GC100.0% 1911.9 / NA 488V137L (GTG→CTG) PA14_RS13175leucine‑rich repeat‑containing protein kinase family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (218/270);  total (218/270)

Only 100 of 499 total aligned reads displayed.

GCCGACTCCCTGGAGGTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGGTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGCAGAAGCTGATG  >  NC_008463/2807119‑2807412
                                                                                                                                                   |                                                                                                                                                  
gCCGACTCCCTGGAGGTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTg                                                                                                                                                  <  2:1897423/150‑1 (MQ=255)
  cGACTCCCTGGAGGTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAg                                                                                                                                                <  1:4320463/150‑1 (MQ=255)
   gACTCCCTGGAGGTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGc                                                                                                                                               >  2:5011381/1‑150 (MQ=255)
     cTCCCTGGAGGTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCAt                                                                                                                                             <  1:4052502/150‑1 (MQ=255)
     cTCCCTGGAGGTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCAt                                                                                                                                             >  1:6275410/1‑150 (MQ=255)
          tGGAGGTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCg                                                                                                                                        >  2:4162992/1‑150 (MQ=255)
          tGGAGGTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCg                                                                                                                                        <  1:1956132/150‑1 (MQ=255)
                  cTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAg                                                                                                                                >  2:286017/1‑150 (MQ=255)
                   tCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGt                                                                                                                               <  1:609218/150‑1 (MQ=255)
                    cAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTc                                                                                                                              <  1:3949592/150‑1 (MQ=255)
                    cAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTc                                                                                                                              >  1:8275394/1‑150 (MQ=255)
                      aCCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCa                                                                                                                            >  2:8208778/1‑150 (MQ=255)
                             cGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCAt                                                                                                                     >  1:10707862/1‑150 (MQ=255)
                             cGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCAt                                                                                                                     <  2:7479984/150‑1 (MQ=255)
                              ggCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATc                                                                                                                    <  2:5194082/150‑1 (MQ=255)
                              ggCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATc                                                                                                                    <  2:2665359/150‑1 (MQ=255)
                                 aaCGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAg                                                                                                                 <  2:6883368/150‑1 (MQ=255)
                                   cGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCg                                                                                                               <  2:5726023/150‑1 (MQ=255)
                                     cccTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCggg                                                                                                             <  2:8061190/150‑1 (MQ=255)
                                      ccTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGt                                                                                                            <  1:3069601/150‑1 (MQ=255)
                                      ccTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGt                                                                                                            <  2:322664/150‑1 (MQ=255)
                                       cTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTg                                                                                                           >  2:7326627/1‑150 (MQ=255)
                                       cTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTg                                                                                                           <  1:506629/150‑1 (MQ=255)
                                       cTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTg                                                                                                           >  1:4578281/1‑150 (MQ=255)
                                         ggACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTgcc                                                                                                         >  1:5688763/1‑150 (MQ=255)
                                          gACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTgccg                                                                                                        <  2:8684798/150‑1 (MQ=255)
                                              gCCTTCAGGACGACCTCGGCCGGCTGCGTCGTCTCAAGGTGCTGTACTGTTCCGGGAAAGACGTCGACGAATAGCATGGGGCGCTGGGCGAAGGTCCGGCGCTGAGGCTGGTCGGCGGGCTGTACGGGCGCCTCGCGCGGGTgccggccg                                                                                                    >  2:9391799/1‑150 (MQ=255)
                                              gCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTgccggccg                                                                                                    >  1:5847994/1‑150 (MQ=255)
                                               ccTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCcgc                                                                                                   >  1:5339445/1‑150 (MQ=255)
                                                    cGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGccc                                                                                              <  1:9174028/150‑1 (MQ=255)
                                                     ggACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACGGTCCGGCGCTGAGCATGGTCGGCGTCTAGTCCAAGCGCATCGAGCGGGTGCCGGCCGCGGcccg                                                                                             >  1:2024651/1‑149 (MQ=255)
                                                       acgacCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTgc                                                                                           <  2:1693821/150‑1 (MQ=255)
                                                       acgacCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTgc                                                                                           <  1:461854/150‑1 (MQ=255)
                                                          acCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTgccgc                                                                                        <  1:5182034/150‑1 (MQ=255)
                                                          acCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTgccgc                                                                                        <  1:7219458/150‑1 (MQ=255)
                                                          acCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAAGTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTgccgc                                                                                        <  2:8503777/150‑1 (MQ=255)
                                                           ccTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTgccgcc                                                                                       <  1:4778390/150‑1 (MQ=255)
                                                              cggccggcTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGc                                                                                    <  1:6407567/150‑1 (MQ=255)
                                                              cggccggcTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGc                                                                                    >  2:2507998/1‑150 (MQ=255)
                                                                  cggcTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTg                                                                                <  2:10046160/150‑1 (MQ=255)
                                                                   ggcTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGACGCGGCCCTGCCGCCGGCGTTgc                                                                               <  2:7410388/150‑1 (MQ=255)
                                                                     cTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTgcgc                                                                             <  1:6894750/150‑1 (MQ=255)
                                                                     cTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTgcgc                                                                             >  1:4455542/1‑150 (MQ=255)
                                                                      tGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGCCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCgct                                                                            >  1:6652069/1‑150 (MQ=255)
                                                                       gCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCgctg                                                                           <  2:55449/150‑1 (MQ=255)
                                                                       gCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCgctg                                                                           >  2:4801847/1‑150 (MQ=255)
                                                                         aTCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCgctggc                                                                         <  2:10415994/150‑1 (MQ=255)
                                                                         aTCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCgctggc                                                                         <  1:1160450/150‑1 (MQ=255)
                                                                          tCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCgctggct                                                                        >  2:10728489/1‑150 (MQ=255)
                                                                            gTCTCAAGGTGCTGTTCTGTTCCGCCAACGAAGTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTTCCGGCCGCGGCCCTGCCTCCGGCGTTGCGCTGGCTGa                                                                      <  2:1151639/150‑1 (MQ=255)
                                                                             tctcAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGAt                                                                     >  1:2931715/1‑150 (MQ=255)
                                                                             tctcAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGAt                                                                     <  1:1587549/150‑1 (MQ=255)
                                                                              ctcAAGGTGCTGTTCTGGTCCGCCAACGACTTCGCCGACTTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATc                                                                    <  2:7157144/150‑1 (MQ=255)
                                                                                 aaGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTc                                                                 >  2:6436259/1‑150 (MQ=255)
                                                                                   ggTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCAc                                                               <  1:9698486/150‑1 (MQ=255)
                                                                                   ggTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCAc                                                               <  2:7825109/150‑1 (MQ=255)
                                                                                      gCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGa                                                            >  2:9936576/1‑150 (MQ=255)
                                                                                           tctgttcCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAAcc                                                       <  2:10154253/150‑1 (MQ=255)
                                                                                           tctgttcCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAAcc                                                       <  1:4935055/150‑1 (MQ=255)
                                                                                            ctgttcCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCg                                                      <  2:2342834/150‑1 (MQ=255)
                                                                                             tgttcCGCCAACGACTTCGCCGAAGTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCgg                                                     <  1:7430971/150‑1 (MQ=255)
                                                                                               ttcCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGAt                                                   <  1:10378487/150‑1 (MQ=255)
                                                                                               ttcCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGAt                                                   >  1:352830/1‑150 (MQ=255)
                                                                                               ttcCGCCAACGACTTCGCCGAAGTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGAt                                                   <  2:5880898/150‑1 (MQ=255)
                                                                                                  cGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGc                                                >  1:4535895/1‑150 (MQ=255)
                                                                                                        cGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCt                                          <  1:10010101/150‑1 (MQ=255)
                                                                                                          aCTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCCTGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGc                                        >  1:3197802/1‑150 (MQ=255)
                                                                                                          aCTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCATCCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGGTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGc                                        >  1:9374769/1‑150 (MQ=255)
                                                                                                          aCTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGc                                        <  2:6591808/150‑1 (MQ=255)
                                                                                                          aCTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGGGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGc                                        <  2:4634782/150‑1 (MQ=255)
                                                                                                           cTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGcc                                       <  1:9090725/150‑1 (MQ=255)
                                                                                                             tCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCg                                     <  2:1480979/150‑1 (MQ=255)
                                                                                                             tCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCg                                     <  2:626741/150‑1 (MQ=255)
                                                                                                               gCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACACCCGGATTGCCACGCTGCCCGAg                                   >  2:8806865/1‑150 (MQ=255)
                                                                                                                ccGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAgg                                  >  1:266145/1‑150 (MQ=255)
                                                                                                                 cGAAGTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGa                                 <  2:215846/150‑1 (MQ=255)
                                                                                                                  gAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGac                                >  2:10658997/1‑149 (MQ=255)
                                                                                                                  gAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGaa                                >  1:133310/1‑150 (MQ=255)
                                                                                                                      tGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTgg                            <  1:7088764/150‑1 (MQ=255)
                                                                                                                      tGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTgg                            <  1:5605454/150‑1 (MQ=255)
                                                                                                                         cggcggcggTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCAGCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTc                         <  2:3964718/150‑1 (MQ=255)
                                                                                                                          ggcggcggTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCg                        >  1:10167017/1‑150 (MQ=255)
                                                                                                                           gcggcggTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCgc                       <  1:4086133/150‑1 (MQ=255)
                                                                                                                            cggcggTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCgcc                      <  2:426212/150‑1 (MQ=255)
                                                                                                                            cggcggTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCgcc                      >  2:6094077/1‑150 (MQ=255)
                                                                                                                            cggcggTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCgcc                      <  2:3540408/150‑1 (MQ=255)
                                                                                                                               cggTGGGCGACTGTCCGGCGCTGAGCATGGTCGGATTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCgccggc                   <  2:6854127/150‑1 (MQ=255)
                                                                                                                                    ggCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTg              <  1:8674984/150‑1 (MQ=255)
                                                                                                                                     gCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGc             <  1:10068137/150‑1 (MQ=255)
                                                                                                                                      cGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGCa            <  1:1227336/150‑1 (MQ=255)
                                                                                                                                         cTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGCAGaa         <  2:4545188/150‑1 (MQ=255)
                                                                                                                                          tGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGCAGAAg        >  2:3651681/1‑150 (MQ=255)
                                                                                                                                           gTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGCAGAAGc       <  1:8228263/150‑1 (MQ=255)
                                                                                                                                            tCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGCAGAAGCt      >  1:780431/1‑150 (MQ=255)
                                                                                                                                              cggcgcTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGCAGAAGCTGa    >  2:3096808/1‑150 (MQ=255)
                                                                                                                                               ggcgcTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACCACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGCAGAAGCGGAt   >  1:7025025/1‑150 (MQ=255)
                                                                                                                                               ggcgcTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGCAGAAGCTGAt   <  1:241423/150‑1 (MQ=255)
                                                                                                                                               ggcgcTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGCAGAAGCTGAt   >  2:4081824/1‑150 (MQ=255)
                                                                                                                                               ggcgcTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGCAGAAGCTGAt   >  2:4395509/1‑150 (MQ=255)
                                                                                                                                                gcgcTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGCAGAAGCTGATg  <  2:9165920/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCCGACTCCCTGGAGGTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGGTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGCAGAAGCTGATG  >  NC_008463/2807119‑2807412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑