Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,840,442 T→C intergenic (+42/‑47) PA14_RS13335 → / → PA14_RS13340 LysR substrate‑binding domain‑containing protein/sigma‑70 family RNA polymerase sigma factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,840,4420TC100.0% 2058.2 / NA 519intergenic (+42/‑47)PA14_RS13335/PA14_RS13340LysR substrate‑binding domain‑containing protein/sigma‑70 family RNA polymerase sigma factor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (264/255);  total (264/255)

Only 100 of 519 total aligned reads displayed.

CGGCGAGGGCGCGGTGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTTTTGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTGCGCGGCAAGCTG  >  NC_008463/2840298‑2840587
                                                                                                                                                |                                                                                                                                                 
cGGCGAGGGCGCGGTGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGAc                                                                                                                                              <  1:2693682/150‑1 (MQ=255)
cGGCGAGGGCGCGGTGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGAc                                                                                                                                              <  1:2179230/150‑1 (MQ=255)
cGGCGAGGGCGCGGTGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGAc                                                                                                                                              <  1:7636271/150‑1 (MQ=255)
    gAGGGCGCGGTGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATg                                                                                                                                          >  2:6273721/1‑150 (MQ=255)
      gggCGCGGTGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCg                                                                                                                                        <  1:4276077/150‑1 (MQ=255)
       ggCGCGGTGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGa                                                                                                                                       <  1:6578411/150‑1 (MQ=255)
        gcgcGGTGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGAt                                                                                                                                      <  1:8475085/150‑1 (MQ=255)
         cgcgGTGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGAtt                                                                                                                                     <  1:5017928/150‑1 (MQ=255)
         cgcgGTGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGAtt                                                                                                                                     <  1:3275062/150‑1 (MQ=255)
            ggTGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCAt                                                                                                                                  >  1:4686427/1‑150 (MQ=255)
               gggTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTAt                                                                                                                               >  1:3687515/1‑150 (MQ=255)
                  tATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTAtcat                                                                                                                            <  1:9274635/150‑1 (MQ=255)
                  tATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTAtcat                                                                                                                            >  2:1981203/1‑150 (MQ=255)
                    tgtgCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTAtcattc                                                                                                                          <  2:10078783/150‑1 (MQ=255)
                    tgtgCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTAtcattc                                                                                                                          <  2:9087154/150‑1 (MQ=255)
                     gtgCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTAtcattca                                                                                                                         <  1:6382751/150‑1 (MQ=255)
                      tgCTGGCGCGCCGAACCGCAGCGCGACCGCGCCCGCGAACACCTGCTGGCCTGCCGGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGAGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTAtcattcat                                                                                                                        <  2:8938836/150‑1 (MQ=255)
                      tgCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGGAGGGCGCCGGCCGGCGGGTGCTATTGCACAAAGGCCGCGGCTTCTCGACAATGCGATTCATTAtcagtcat                                                                                                                        >  2:294412/1‑150 (MQ=255)
                       gCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCAtg                                                                                                                       >  1:2761290/1‑150 (MQ=255)
                         tGGCGCGACGAACCGCATCGCGACCGCGCCCGCGACGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTAGTGCAAACAGGCCGCGGCTTCTCGACATTGCGAGTCATTCTcatgcatggg                                                                                                                     >  2:4006184/1‑148 (MQ=255)
                               gacgaACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGccc                                                                                                               >  2:104475/1‑150 (MQ=255)
                                acgaACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGcccc                                                                                                              >  1:9876443/1‑150 (MQ=255)
                                 cgaACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCg                                                                                                             >  1:2946682/1‑150 (MQ=255)
                                 cgaACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCg                                                                                                             <  2:7207886/150‑1 (MQ=255)
                                   aaCCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGAc                                                                                                           >  2:4439389/1‑150 (MQ=255)
                                     ccGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACtt                                                                                                         >  2:3126482/1‑150 (MQ=255)
                                       gCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTc                                                                                                       >  2:7414820/1‑150 (MQ=255)
                                       gCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTc                                                                                                       <  2:8105153/150‑1 (MQ=255)
                                          tCGCGACCGCGCCAGCGTAGACCTGCGGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCa                                                                                                    <  2:8589863/150‑1 (MQ=255)
                                             cgACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGt                                                                                                 <  1:3229359/150‑1 (MQ=255)
                                             cgACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGt                                                                                                 <  2:2827195/150‑1 (MQ=255)
                                              gACCGCGCCAGCGAAGACCGGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGGAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCAGGTc                                                                                                >  2:2370434/1‑150 (MQ=255)
                                               aCCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTcc                                                                                               >  1:1078753/1‑150 (MQ=255)
                                               aCCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTcc                                                                                               <  2:3057749/150‑1 (MQ=255)
                                               aCCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTcc                                                                                               >  2:969810/1‑150 (MQ=255)
                                               aCCGCGCCAGCGAAGACCTGCTGGCCAGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTcc                                                                                               >  1:3450421/1‑150 (MQ=255)
                                                ccGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCg                                                                                              >  1:9451819/1‑150 (MQ=255)
                                                 cgcgCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGc                                                                                             <  1:4022301/150‑1 (MQ=255)
                                                 cgcgCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGc                                                                                             >  2:6083146/1‑150 (MQ=255)
                                                   cgcCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATACATGTGTCGCCCCGACTTTCCCATGTCCGccc                                                                                           <  1:6117485/150‑1 (MQ=255)
                                                    gcCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCACAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGcccc                                                                                          <  1:731203/150‑1 (MQ=255)
                                                    gcCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGcccc                                                                                          >  2:7747302/1‑150 (MQ=255)
                                                         cGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATcc                                                                                     <  2:8191573/150‑1 (MQ=255)
                                                         cGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATcc                                                                                     >  1:10000214/1‑150 (MQ=255)
                                                          gAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCt                                                                                    >  1:1344792/1‑150 (MQ=255)
                                                           aaGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCtt                                                                                   <  2:5169702/150‑1 (MQ=255)
                                                             gACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTCGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTGCGCCCCGATCCTtct                                                                                 >  2:4894872/1‑150 (MQ=255)
                                                              aCCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTtctc                                                                                <  1:7912428/150‑1 (MQ=255)
                                                              aCCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTtctc                                                                                <  1:4626888/150‑1 (MQ=255)
                                                              aCCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTtctc                                                                                <  2:5898498/150‑1 (MQ=255)
                                                                ctgctgGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAg                                                                              >  1:8950828/1‑150 (MQ=255)
                                                                ctgctgGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAg                                                                              >  1:10435455/1‑150 (MQ=255)
                                                                 tgctgGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAgc                                                                             <  1:8824344/150‑1 (MQ=255)
                                                                  gctgGCCTGCCTGCGCGAAGGCGCGGCGGCCGTGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTCTCATTCAGGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAgcg                                                                            >  2:1355562/1‑150 (MQ=255)
                                                                         tgcctgcGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCccacca                                                                     <  1:1137198/150‑1 (MQ=255)
                                                                          gcctgcGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAATAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCAt                                                                    <  2:6957917/150‑1 (MQ=255)
                                                                           cctgcGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCCAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATc                                                                   >  2:4133116/1‑150 (MQ=255)
                                                                            ctgcGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATcg                                                                  <  2:9222785/150‑1 (MQ=255)
                                                                             tgcGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATcgc                                                                 >  2:130192/1‑150 (MQ=255)
                                                                               cgcgAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATGGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCgcgc                                                               <  1:3112663/150‑1 (MQ=255)
                                                                                  gAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCcat                                                            >  1:1820527/1‑150 (MQ=255)
                                                                                   aaGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCcatg                                                           >  2:10361852/1‑150 (MQ=255)
                                                                                   aaGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCcatg                                                           >  2:4363896/1‑150 (MQ=255)
                                                                                     ggCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCcatgca                                                         >  2:6712978/1‑150 (MQ=255)
                                                                                      gcgcGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCcatgcat                                                        >  1:7143489/1‑150 (MQ=255)
                                                                                       cgcgGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCcatgcatg                                                       >  2:2616587/1‑150 (MQ=255)
                                                                                        gccgcggcCGAGTAGGGCGCCGGCCGGCGGCTGCTCGTGCAACCAGGCCGCGGCTTCTCGACAAGGCGATTCATTATCATTCATGTGTCGCCCCGACTGTCCCATGTCCGCCCCGATCCTACTCAGCGCCCACCATCGCGCCatgcatgc                                                      <  2:10285664/147‑1 (MQ=255)
                                                                                          ggcggcCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATgcgc                                                    <  1:8407125/150‑1 (MQ=255)
                                                                                             ggcCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGt                                                 >  1:8002528/1‑150 (MQ=255)
                                                                                                cGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACa                                              <  2:10547221/150‑1 (MQ=255)
                                                                                                 gAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAg                                             <  1:3835696/150‑1 (MQ=255)
                                                                                                  aGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGc                                            <  1:9093681/150‑1 (MQ=255)
                                                                                                   gTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCg                                           >  1:6674278/1‑150 (MQ=255)
                                                                                                     aGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAg                                         <  2:1085318/150‑1 (MQ=255)
                                                                                                       ggCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCa                                       >  1:621428/1‑150 (MQ=255)
                                                                                                           ccggccggCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCAt                                   >  1:10105948/1‑150 (MQ=255)
                                                                                                              gccggcGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCTTGCATGCGCTGTACAGCGAGCACCATGGc                                >  2:6550948/1‑150 (MQ=255)
                                                                                                                cggcggGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTg                              >  1:5572815/1‑150 (MQ=255)
                                                                                                                 ggcggGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTgg                             >  1:548508/1‑150 (MQ=255)
                                                                                                                    gggTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTg                          >  2:3701144/1‑150 (MQ=255)
                                                                                                                     ggTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGAACCTTCTCAGCCCCCACCATCGCGCCATGCATGCGCTGAACAGCGAGCACCATGGCTGGTTGc                         >  2:779771/1‑150 (MQ=255)
                                                                                                                        gCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGa                      <  2:572760/150‑1 (MQ=255)
                                                                                                                        gCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGa                      >  1:7037802/1‑150 (MQ=255)
                                                                                                                            ttGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTg                  <  1:574994/150‑1 (MQ=255)
                                                                                                                             tGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTgg                 <  1:8307741/150‑1 (MQ=255)
                                                                                                                              gCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGt                >  1:1782677/1‑150 (MQ=255)
                                                                                                                               cAAAAAGGCCGCGGCTTCTCGACACTGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGtt               <  2:5347373/150‑1 (MQ=255)
                                                                                                                               cAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGtt               >  1:8635811/1‑150 (MQ=255)
                                                                                                                               cAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGtt               <  1:7224152/150‑1 (MQ=255)
                                                                                                                                aaaaaGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTg              >  1:6022036/1‑150 (MQ=255)
                                                                                                                                 aaaaGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTgc             <  1:6851629/150‑1 (MQ=255)
                                                                                                                                   aaGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTgcgc           >  1:3789146/1‑150 (MQ=255)
                                                                                                                                      gCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTGCGCGGc        >  2:3407438/1‑150 (MQ=255)
                                                                                                                                      gCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTGCGCGGc        >  1:4374998/1‑150 (MQ=255)
                                                                                                                                         gcgGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTGCGCGGCAAg     >  1:3575106/1‑150 (MQ=255)
                                                                                                                                          cgGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTGCGCGGCAAGc    <  2:7546860/150‑1 (MQ=255)
                                                                                                                                          cgGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTGCGCGGCAAGc    <  2:4828775/150‑1 (MQ=255)
                                                                                                                                           ggCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTGCGCGGCAAGCt   <  2:3890989/150‑1 (MQ=255)
                                                                                                                                            gCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTGCGCGGCAAGCTg  <  1:2569883/150‑1 (MQ=255)
                                                                                                                                            gCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTGCGCGGCAAGCTg  >  2:9457879/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
CGGCGAGGGCGCGGTGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTTTTGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTGCGCGGCAAGCTG  >  NC_008463/2840298‑2840587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑