Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,985,345 A→G K2233E (AAA→GAA)  pvdD → pyoverdine non‑ribosomal peptide synthetase PvdD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,985,3450AG100.0% 1526.1 / NA 385K2233E (AAA→GAA) pvdDpyoverdine non‑ribosomal peptide synthetase PvdD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (173/212);  total (173/212)

Only 100 of 387 total aligned reads displayed.

TGTTACAAGACCCTCGCCATGGCGCTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCAAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTCAGGGTCGAAGCGTTCTGGAA  >  NC_008463/2985198‑2985490
                                                                                                                                                   |                                                                                                                                                 
  ttACAAGACCCTCGCCATGGCGCTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAgg                                                                                                                                               >  1:2659505/1‑150 (MQ=255)
   tACAAGACCCTCGCCATGGCGCTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAggg                                                                                                                                              >  1:1153449/1‑150 (MQ=255)
   tACAAGACCCTCGCCATGGCGCTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAggg                                                                                                                                              <  1:1523354/150‑1 (MQ=255)
       aGACCCTCGCCATGGCGCTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGttt                                                                                                                                          >  2:9108318/1‑150 (MQ=255)
       aGACCCTCGCCATGGCGCTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGttt                                                                                                                                          <  2:4244049/150‑1 (MQ=255)
       aGACCCTCGCCATGGCGCTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGttt                                                                                                                                          <  1:985896/150‑1 (MQ=255)
        gACCCTCGCCATGGCGCTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGtttt                                                                                                                                         <  1:2382274/150‑1 (MQ=255)
         aCCCTCGCCATGGCGCTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTc                                                                                                                                        <  1:3876380/150‑1 (MQ=255)
         aCCCTCGCCATGGCGCTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTc                                                                                                                                        >  2:5856100/1‑150 (MQ=255)
             tCGCCATGGCGCTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAAcc                                                                                                                                    <  1:5502003/150‑1 (MQ=255)
                ccATGGCGCTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTgg                                                                                                                                 <  2:7046120/150‑1 (MQ=255)
                 cATGGCGCTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGc                                                                                                                                <  2:1565066/150‑1 (MQ=255)
                      cgcTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGAt                                                                                                                           >  1:8727864/1‑150 (MQ=255)
                      cgcTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGAt                                                                                                                           <  2:4107929/150‑1 (MQ=255)
                      cgcTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGGGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGAt                                                                                                                           <  2:7078192/150‑1 (MQ=255)
                         tGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGt                                                                                                                        <  1:1671730/150‑1 (MQ=255)
                          gCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTc                                                                                                                       <  1:2847016/150‑1 (MQ=255)
                           cGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCg                                                                                                                      >  1:5058066/1‑150 (MQ=255)
                            gggATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGTGGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGTGCACCCCGTAGGGGTTTTCAACCTGGCGGGATGGTCGc                                                                                                                     <  1:7539191/150‑1 (MQ=255)
                            gggATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGc                                                                                                                     >  2:9505177/1‑150 (MQ=255)
                                tCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTcgg                                                                                                                 <  2:3865166/150‑1 (MQ=255)
                                 cGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTcggc                                                                                                                <  1:10058583/150‑1 (MQ=255)
                                   tCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTcggcgg                                                                                                              >  2:1262345/1‑150 (MQ=255)
                                      tCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCaa                                                                                                           >  2:8443970/1‑150 (MQ=255)
                                      tCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCaa                                                                                                           <  2:2257779/150‑1 (MQ=255)
                                       ccGGTCAAGGGTGTTGTCTGCCGTGCCCTGCGGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAAc                                                                                                          <  2:7498368/150‑1 (MQ=255)
                                        cGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAAcc                                                                                                         <  2:1739279/150‑1 (MQ=255)
                                        cGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAAcc                                                                                                         <  2:4624778/150‑1 (MQ=255)
                                        cGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAAcc                                                                                                         <  1:7736290/150‑1 (MQ=255)
                                            cAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGc                                                                                                     <  1:3225579/150‑1 (MQ=255)
                                                ggTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCgatg                                                                                                 <  1:2530030/150‑1 (MQ=255)
                                                    ttgtCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCgatggatg                                                                                             <  1:1065493/150‑1 (MQ=255)
                                                    ttgtCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCgatggatg                                                                                             <  1:3698994/150‑1 (MQ=255)
                                                      gtCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTc                                                                                           >  2:80849/1‑150 (MQ=255)
                                                         tGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTcgcg                                                                                        >  1:4933967/1‑150 (MQ=255)
                                                            cGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGcc                                                                                     <  2:10730328/150‑1 (MQ=255)
                                                             gTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGccc                                                                                    <  2:635181/150‑1 (MQ=255)
                                                                  ctgctgGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGTCGAGGGATGTCGCGGCCCGGCTg                                                                               >  2:6015328/1‑150 (MQ=255)
                                                                   tgctgGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTgg                                                                              <  1:2341319/150‑1 (MQ=255)
                                                                    gctgGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGa                                                                             >  2:5220996/1‑150 (MQ=255)
                                                                    gctgGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGa                                                                             <  1:4796300/150‑1 (MQ=255)
                                                                       gggCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGagca                                                                          <  2:2454875/150‑1 (MQ=255)
                                                                             tGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTgg                                                                    >  1:2600417/1‑150 (MQ=255)
                                                                                tCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGggcg                                                                 <  2:8325252/150‑1 (MQ=255)
                                                                                   cgAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCa                                                              >  2:8568799/1‑150 (MQ=255)
                                                                                    gAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAg                                                             >  2:5034563/1‑150 (MQ=255)
                                                                                      ggTGCCGGAGTGGGACGATATGGTTGCGGACTACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGAGGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGt                                                           <  1:630070/150‑1 (MQ=255)
                                                                                       gTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTg                                                          >  2:188154/1‑150 (MQ=255)
                                                                                        tGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTgg                                                         <  1:9688728/150‑1 (MQ=255)
                                                                                           cGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCtt                                                      >  2:3139140/1‑150 (MQ=255)
                                                                                               gTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTtcgt                                                  <  1:7839275/150‑1 (MQ=255)
                                                                                               gTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTtcgt                                                  >  1:1820075/1‑150 (MQ=255)
                                                                                               gTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTtcgt                                                  <  1:9177344/150‑1 (MQ=255)
                                                                                                tGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTtcgtc                                                 <  1:4094699/150‑1 (MQ=255)
                                                                                                  ggACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCgg                                               >  1:8196857/1‑150 (MQ=255)
                                                                                                   gACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGc                                              >  1:7417833/1‑150 (MQ=255)
                                                                                                      gATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTgg                                           <  2:6426436/150‑1 (MQ=255)
                                                                                                         atGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATc                                        >  2:9301288/1‑150 (MQ=255)
                                                                                                         atGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATc                                        >  1:4048583/1‑150 (MQ=255)
                                                                                                         atGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATc                                        <  2:4402278/150‑1 (MQ=255)
                                                                                                          tGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCg                                       >  1:1392056/1‑150 (MQ=255)
                                                                                                          tGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCg                                       >  2:200028/1‑150 (MQ=255)
                                                                                                             ttGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATg                                    <  2:968155/150‑1 (MQ=255)
                                                                                                             ttGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATg                                    >  1:4401254/1‑150 (MQ=255)
                                                                                                             gtGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATg                                    <  2:257554/149‑1 (MQ=255)
                                                                                                              tGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATgc                                   >  1:1050173/1‑150 (MQ=255)
                                                                                                              tGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATgc                                   >  1:7931795/1‑150 (MQ=255)
                                                                                                                cGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATgcgc                                 <  2:5339060/150‑1 (MQ=255)
                                                                                                                  gAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCg                               >  2:7890629/1‑150 (MQ=255)
                                                                                                                  gAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCg                               >  2:2906015/1‑150 (MQ=255)
                                                                                                                  gAATACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCg                               >  1:3017783/1‑150 (MQ=255)
                                                                                                                    aTACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGc                             >  2:7743873/1‑150 (MQ=255)
                                                                                                                     tACGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCa                            >  2:3441199/1‑150 (MQ=255)
                                                                                                                      aCGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCAc                           <  2:5650730/150‑1 (MQ=255)
                                                                                                                       cGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCAcc                          <  1:8428138/150‑1 (MQ=255)
                                                                                                                       cGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCAcc                          >  2:6237372/1‑150 (MQ=255)
                                                                                                                       cGCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCAcc                          >  2:6122379/1‑150 (MQ=255)
                                                                                                                        gCCGAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCg                         >  1:9455159/1‑150 (MQ=255)
                                                                                                                           gAGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTc                      >  1:5379219/1‑150 (MQ=255)
                                                                                                                            aGCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTCa                     >  1:1704494/1‑150 (MQ=255)
                                                                                                                             gCAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTCAg                    <  1:417039/150‑1 (MQ=255)
                                                                                                                              cAATTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTCAgg                   <  2:731865/150‑1 (MQ=255)
                                                                                                                               aaTTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTCAggg                  <  2:9142132/150‑1 (MQ=255)
                                                                                                                                aTTGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTCAGGGt                 <  1:10149078/150‑1 (MQ=255)
                                                                                                                                 ttGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTCAGGGTc                >  1:6626973/1‑150 (MQ=255)
                                                                                                                                 ttGCTGCAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTCAGGGTc                >  2:1291655/1‑150 (MQ=255)
                                                                                                                                   gctgcAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTCAGGGTCGa              >  1:9036803/1‑150 (MQ=255)
                                                                                                                                     tgcAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTCAGGGTCGAAg            <  1:6167284/150‑1 (MQ=255)
                                                                                                                                      gcAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTCAGGGTCGAAGc           >  2:6650467/1‑150 (MQ=255)
                                                                                                                                      gcAGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTCAGGGTCGAAGc           <  2:3039173/150‑1 (MQ=255)
                                                                                                                                        aGGAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTCAGGGTCGAAGCGt         >  2:10576479/1‑150 (MQ=255)
                                                                                                                                         ggAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTCAGGGTCGAAGCGtt        <  1:5205653/150‑1 (MQ=255)
                                                                                                                                         ggAGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTCAGGGTCGAAGCGtt        >  2:8794462/1‑150 (MQ=255)
                                                                                                                                           aGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTCAGGGTCGAAGCGTTCt      >  1:5635282/1‑150 (MQ=255)
                                                                                                                                           aGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTCAGGGTCGAAGCGTTCt      >  2:6580489/1‑150 (MQ=255)
                                                                                                                                           aGCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTCAGGGTCGAAGCGTTCt      >  1:7115633/1‑150 (MQ=255)
                                                                                                                                            gCACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTCAGGGTCGAAGCGTTCTg     >  1:575558/1‑150 (MQ=255)
                                                                                                                                             cACCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTCAGGGTCGAAGCGTTCTgg    >  1:6939070/1‑150 (MQ=255)
                                                                                                                                              aCCCCGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTCAGGGTCGAAGCGTTCTGGa   >  2:10222988/1‑150 (MQ=255)
                                                                                                                                               ccccGAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTCAGGGTCGAAGCGTTCTGGaa  <  1:3463538/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TGTTACAAGACCCTCGCCATGGCGCTGCGGGATCGTCATCCGGTCAAGGGTGTTGTCTGCCGTGCCCTGCTGGGCGCTGGTCGCGAGGTGCCGGAGTGGGACGATATGGTTGCGGAATACGCCGAGCAATTGCTGCAGGAGCACCCCAAAGGGGTTTTCAACCTGGCGGGATGGTCGCTCGGCGGCAACCTGGCGATGGATGTCGCGGCCCGGCTGGAGCAGCGTGGGCGGCAGGTGGCTTTCGTCGGCTGGATCGATGCGCCGGCACCGGTCAGGGTCGAAGCGTTCTGGAA  >  NC_008463/2985198‑2985490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑