Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,198,441 T→G A547A (GCA→GCC)  PA14_RS14615 ← acyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,198,4410TG99.8% 1667.3 / NA 424A547A (GCA→GCC) PA14_RS14615acyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base G (220/203);  total (221/203)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 429 total aligned reads displayed.

TCAGGGTGAAACGGTTTCGGCGCGCCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCTGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGCACCAGCGGACCAG  >  NC_008463/3198294‑3198587
                                                                                                                                                   |                                                                                                                                                  
 cAGGGTGAAACGGTTTCGGCGCGCCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCg                                                                                                                                                 >  1:9531978/1‑150 (MQ=255)
   gggTGAAACGGTTTCGGCGCGCCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTc                                                                                                                                               >  1:6756325/1‑150 (MQ=255)
   gggTGAAACGGTTTCGGCGCGCCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTc                                                                                                                                               <  2:6743908/150‑1 (MQ=255)
   gggTGAAACGGTTTCGGCGCGCCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTc                                                                                                                                               >  1:3612092/1‑150 (MQ=255)
   gggTGAAACGGTTTCGGCGCGCCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTc                                                                                                                                               <  2:317192/150‑1 (MQ=255)
          aCGGTTTCGGCGCGCCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGcc                                                                                                                                        <  2:8689476/150‑1 (MQ=255)
          aCGGTTTCGGCGCGCCACCAGGTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGcc                                                                                                                                        <  2:6799569/150‑1 (MQ=255)
            ggTTTCGGCGCGCCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCAGCAGCGGCCGGCGTCGCCGGcctc                                                                                                                                      >  2:10368756/1‑150 (MQ=255)
                 cGGCGCGCCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGcctcctcct                                                                                                                                 <  2:212164/150‑1 (MQ=255)
                     gcgcCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTg                                                                                                                             >  1:9004569/1‑150 (MQ=255)
                      cgcCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGc                                                                                                                            <  2:9739678/150‑1 (MQ=255)
                       gcCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGcc                                                                                                                           >  2:5180830/1‑150 (MQ=255)
                        ccaccaGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCg                                                                                                                          >  1:10182573/1‑150 (MQ=255)
                        ccaccaGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCg                                                                                                                          >  1:4070835/1‑150 (MQ=255)
                         caccaGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCgg                                                                                                                         <  2:3665755/150‑1 (MQ=255)
                          accaGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCggg                                                                                                                        >  1:10695541/1‑150 (MQ=255)
                          accaGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCggg                                                                                                                        >  1:5307690/1‑150 (MQ=255)
                             cgTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGccc                                                                                                                     <  1:9947308/149‑1 (MQ=255)
                              gTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCa                                                                                                                    <  2:3553072/150‑1 (MQ=255)
                              gTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCa                                                                                                                    <  1:8253585/150‑1 (MQ=255)
                              gTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCa                                                                                                                    >  1:6127187/1‑150 (MQ=255)
                               gtGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGAGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCAc                                                                                                                   <  2:5556851/149‑1 (MQ=255)
                                tGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACt                                                                                                                  <  1:715003/150‑1 (MQ=255)
                                   tGGAACCGGGCACTGGCGCGGATACGTGCGTCTAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGGTAGGGAATGTGCGGGTGCGGCAGCGCCATCCGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTcgc                                                                                                               <  2:601765/150‑1 (MQ=255)
                                    ggAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTcgcg                                                                                                              >  1:10398756/1‑150 (MQ=255)
                                       aCCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAg                                                                                                           <  2:9158574/150‑1 (MQ=255)
                                            gCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGa                                                                                                      <  2:6162280/150‑1 (MQ=255)
                                                 ggCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTc                                                                                                 >  2:8194882/1‑150 (MQ=255)
                                                  gcgcGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGACCTTCt                                                                                                >  2:6499466/1‑150 (MQ=255)
                                                   cgcgGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTg                                                                                               <  2:5718621/150‑1 (MQ=255)
                                                    gcgGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTgg                                                                                              >  2:6274579/1‑150 (MQ=255)
                                                     cgGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGa                                                                                             >  2:10572537/1‑150 (MQ=255)
                                                     cgGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGa                                                                                             >  2:3819861/1‑150 (MQ=255)
                                                        aTACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCt                                                                                          <  2:7060048/150‑1 (MQ=255)
                                                          aCGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTg                                                                                        <  1:653245/150‑1 (MQ=255)
                                                           cGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGc                                                                                       <  2:2433130/150‑1 (MQ=255)
                                                            gTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGcc                                                                                      >  1:9671514/1‑150 (MQ=255)
                                                            gTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGcc                                                                                      >  1:2027876/1‑150 (MQ=255)
                                                             tGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCg                                                                                     >  2:7981061/1‑150 (MQ=255)
                                                               cggataGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGt                                                                                   >  2:1118148/4‑150 (MQ=255)
                                                               cGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGt                                                                                   <  1:2168868/150‑1 (MQ=255)
                                                               cGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGt                                                                                   <  1:858722/150‑1 (MQ=255)
                                                                 tataGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTgg                                                                                 >  1:2554779/1‑150 (MQ=255)
                                                                 tataGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACACGCGCAGGACGAACTTCTGGATCTAGCCGGAgg                                                                                 >  1:4449012/1‑150 (MQ=255)
                                                                  ataGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGc                                                                                <  2:6896261/150‑1 (MQ=255)
                                                                    aGCAGGCTGGACATGGGCCACCTCGGGCAACCTAGGGATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCgg                                                                              >  2:7386190/1‑150 (MQ=255)
                                                                    aGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCgg                                                                              >  1:7269091/1‑150 (MQ=255)
                                                                     gCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGt                                                                             >  2:2764834/1‑150 (MQ=255)
                                                                       aGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCt                                                                           <  1:7949911/150‑1 (MQ=255)
                                                                       aGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCt                                                                           >  1:5863657/1‑150 (MQ=255)
                                                                        ggCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCtt                                                                          >  2:4082665/1‑150 (MQ=255)
                                                                          cTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTgg                                                                        <  1:8747309/150‑1 (MQ=255)
                                                                                aTGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGtt                                                                  <  2:9628851/150‑1 (MQ=255)
                                                                                aTGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGtt                                                                  >  1:2392344/1‑150 (MQ=255)
                                                                                aTGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGtt                                                                  >  1:7538480/1‑150 (MQ=255)
                                                                                 tGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTc                                                                 <  1:8028221/150‑1 (MQ=255)
                                                                                      cacCTCGGGCAACCAAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCCGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTaa                                                            <  2:4959252/150‑1 (MQ=255)
                                                                                       acCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAg                                                           >  2:10618838/1‑150 (MQ=255)
                                                                                       acCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAg                                                           <  1:5966545/150‑1 (MQ=255)
                                                                                       acCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAg                                                           <  1:4500639/150‑1 (MQ=255)
                                                                                        ccTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGc                                                          >  1:9859098/1‑150 (MQ=255)
                                                                                          tCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGt                                                        <  2:7672203/150‑1 (MQ=255)
                                                                                           cGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTc                                                       <  1:4681137/150‑1 (MQ=255)
                                                                                                   cTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCcg                                               <  1:3908534/150‑1 (MQ=255)
                                                                                                    gaGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCcgc                                              <  2:2295759/149‑1 (MQ=255)
                                                                                                      gTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCgcgg                                            <  2:7087752/150‑1 (MQ=255)
                                                                                                       ttATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCgcggc                                           <  1:7442905/150‑1 (MQ=255)
                                                                                                       ttATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCgcggc                                           <  2:4518363/150‑1 (MQ=255)
                                                                                                          ttGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGcc                                        >  1:2688212/1‑150 (MQ=255)
                                                                                                            gTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCtt                                      <  1:5042316/150‑1 (MQ=255)
                                                                                                            gTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCtt                                      <  2:4580062/150‑1 (MQ=255)
                                                                                                              tATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCTGGACGAACTGCTGGATCTTGCCGGTGGCGGTCTGGGGCAGTTCGGTAAGCGTCACTTGCCGCGGCGCCTGGa                                    >  1:1607419/1‑150 (MQ=255)
                                                                                                               aTGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGaa                                   >  1:5144341/1‑150 (MQ=255)
                                                                                                               aTGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGaa                                   >  2:8808126/1‑150 (MQ=255)
                                                                                                               aTGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGaa                                   >  1:3367136/1‑150 (MQ=255)
                                                                                                                tGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAg                                  <  2:8457270/150‑1 (MQ=255)
                                                                                                                  gAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTg                                >  2:5959000/1‑150 (MQ=255)
                                                                                                                       tgcgggtgcggCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTACGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCa                           >  2:8996379/1‑150 (MQ=255)
                                                                                                                         cgggtgcggCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAgg                         >  1:1881996/1‑150 (MQ=255)
                                                                                                                          gggtgcggCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGgc                        >  1:3215299/1‑150 (MQ=255)
                                                                                                                           ggtgcggCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGgcg                       >  1:1293640/1‑150 (MQ=255)
                                                                                                                            gtgcggCAGCGCCATCAGCGGCCGGCTTCGCCGGCCTCCACCTCCTGCCGGGCCCACTCTCGCAGGAAGAACTTCTGGAGCTTGCCGGTGGCGGTCTGGTGCAGTTCGGTAAGCGTCACATGCCGAGGCGCCTTGAAGAGCGCCCGgcgc                      >  2:400991/1‑150 (MQ=255)
                                                                                                                             tgcggCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCt                     >  1:2811680/1‑150 (MQ=255)
                                                                                                                             tgcggCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCt                     <  2:428855/150‑1 (MQ=255)
                                                                                                                              gcggCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTc                    <  2:1508021/150‑1 (MQ=255)
                                                                                                                              gcggCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTc                    >  1:1698419/1‑150 (MQ=255)
                                                                                                                                 gCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTcgcg                 >  1:4211341/1‑150 (MQ=255)
                                                                                                                                  cAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCgg                >  2:2543767/1‑150 (MQ=255)
                                                                                                                                    gcgcCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGCa              >  2:7523995/1‑150 (MQ=255)
                                                                                                                                    gcgcCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGCa              <  2:9383966/150‑1 (MQ=255)
                                                                                                                                     cgcCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGCAc             >  1:4842232/1‑150 (MQ=255)
                                                                                                                                        cATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGCACCAg          <  1:5024523/150‑1 (MQ=255)
                                                                                                                                         aTCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGCACCAGc         <  1:5545590/150‑1 (MQ=255)
                                                                                                                                          tCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGCACCAGCg        >  2:1295823/1‑150 (MQ=255)
                                                                                                                                           cAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGCACCAGCgg       <  1:3035292/150‑1 (MQ=255)
                                                                                                                                            aGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGCACCAGCGGa      <  1:6832627/150‑1 (MQ=255)
                                                                                                                                             gCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGCCCCAGCGGAc     >  1:5494761/1‑150 (MQ=255)
                                                                                                                                             gCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGCACCAGCggcc     <  1:621502/150‑3 (MQ=255)
                                                                                                                                             gCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTGGAAGTGCGCCAGGCGCTCGCGGCACCAGCGGAc     >  2:5463288/1‑150 (MQ=255)
                                                                                                                                               ggCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGCACCAGCGGACCa   >  1:10535815/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TCAGGGTGAAACGGTTTCGGCGCGCCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCTGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGCACCAGCGGACCAG  >  NC_008463/3198294‑3198587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑