Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,281,477 G→C intergenic (‑25/+44) katE ← / ← PA14_RS14985 catalase HPII/general stress protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,281,4770GC100.0% 1433.7 / NA 358intergenic (‑25/+44)katE/PA14_RS14985catalase HPII/general stress protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (164/194);  total (164/194)

Only 100 of 368 total aligned reads displayed.

GGCGTCCTCGCGATAGGCCTCCAGCTGTTCCAGCTTGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGGGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCTTGAAGTTGCCGCCGCTGGCCTGGCCGCCCTTCTTG  >  NC_008463/3281330‑3281623
                                                                                                                                                   |                                                                                                                                                  
   gTCCTCGCGATAGGCCTCCAGCTGTTCCAGCTTGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATg                                                                                                                                               <  1:3628721/150‑1 (MQ=255)
      cTCGCGATAGGCCTCCAGCTGTTCCAGCTTGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCt                                                                                                                                            <  1:8223243/150‑1 (MQ=255)
      cTCGCGATAGGCCTCCAGCTGTTCCAGCTTGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCt                                                                                                                                            <  1:7421262/150‑1 (MQ=255)
         gcgATAGGCCTCCAGCTGTTCCAGCTTGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTccc                                                                                                                                         <  2:9079666/150‑1 (MQ=255)
          cgATAGGCCTCCAGCTGTTCCAGCTTGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCt                                                                                                                                        <  2:8071060/150‑1 (MQ=255)
              aGGCCTCCCGCTGTTCCAGCTGGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTc                                                                                                                                    <  2:1397234/150‑1 (MQ=255)
              aGGCCTCCAGCTGTTCCAGCTTGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTc                                                                                                                                    >  1:3000042/1‑150 (MQ=255)
               ggCCTCCAGCTGTTCCAGCTTGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTcc                                                                                                                                   >  1:10003587/1‑150 (MQ=255)
               ggCCTCCAGCTGTTCCAGCTTGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTcc                                                                                                                                   >  1:8454845/1‑150 (MQ=255)
               ggCCTCCAGCTGTTCCAGCTTGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTcc                                                                                                                                   <  2:1491274/150‑1 (MQ=255)
                gCCTCCAGCTGTTCCAGCTTGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCg                                                                                                                                  >  1:5590358/1‑150 (MQ=255)
                 ccTCCAGCTGTTCCAGCTTGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGc                                                                                                                                 <  1:1177068/150‑1 (MQ=255)
                 ccTCCAGCTGTTCCAGCTTGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGc                                                                                                                                 <  1:6601188/150‑1 (MQ=255)
                      aGCTGTTCCAGCTTGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCAt                                                                                                                            <  2:1687127/150‑1 (MQ=255)
                      aGCTGTTCCAGCTTGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCAt                                                                                                                            <  1:3336667/150‑1 (MQ=255)
                       gCTGTTCCAGCTTGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCAtt                                                                                                                           >  1:4239081/1‑150 (MQ=255)
                       gCTGTTCCAGCTTGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCAtt                                                                                                                           <  1:2421340/150‑1 (MQ=255)
                         tGTTCCAGCTTGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCAttca                                                                                                                         <  2:8702614/150‑1 (MQ=255)
                         cgTTCCAGCTTGGCGGTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCAttca                                                                                                                         <  2:6893342/149‑1 (MQ=255)
                          gTTCCAGCTTGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCAttcac                                                                                                                        <  2:5250437/150‑1 (MQ=255)
                           ttCCAGCTTGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCAttcact                                                                                                                       <  1:7015174/150‑1 (MQ=255)
                            tCCAGCTTGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCAgtcactt                                                                                                                      >  1:6519652/1‑150 (MQ=255)
                             ccAGCTTGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCCTCCTGGGCATGACAATGGGGCGGATGCCTCCCTGCTCCGCCCCAttcacttc                                                                                                                     <  1:2837741/150‑1 (MQ=255)
                               aGCTTGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCAttcacttcac                                                                                                                   <  2:5412982/150‑1 (MQ=255)
                                gCTTGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATtcacttcact                                                                                                                  <  1:5955663/150‑1 (MQ=255)
                                gCTTGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATtcacttcact                                                                                                                  >  1:9110640/1‑150 (MQ=255)
                                  ttGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTgc                                                                                                                >  1:5353194/1‑150 (MQ=255)
                                   tGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGGTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTgcg                                                                                                               <  2:7668833/150‑1 (MQ=255)
                                        ttGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGtt                                                                                                          <  2:269408/150‑1 (MQ=255)
                                         tGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTa                                                                                                         <  1:4208328/150‑1 (MQ=255)
                                                 ccACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTcc                                                                                                 <  1:3525874/150‑1 (MQ=255)
                                                    cGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCccgc                                                                                              >  2:3515405/1‑150 (MQ=255)
                                                    cGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTGCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCccgc                                                                                              >  1:10163956/1‑150 (MQ=255)
                                                    cGGGCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCccgc                                                                                              <  1:9897799/150‑1 (MQ=255)
                                                      gTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCccgccg                                                                                            <  1:5457461/150‑1 (MQ=255)
                                                      gTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCccgccg                                                                                            <  2:6377774/150‑1 (MQ=255)
                                                       tCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGt                                                                                           >  2:5991202/1‑150 (MQ=255)
                                                       tCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGt                                                                                           <  1:3210720/150‑1 (MQ=255)
                                                        cGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTg                                                                                          <  1:7821700/150‑1 (MQ=255)
                                                         gACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTgg                                                                                         >  2:4451345/1‑150 (MQ=255)
                                                          aCGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCCTCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGc                                                                                        <  1:156263/150‑1 (MQ=255)
                                                          aCGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGc                                                                                        <  1:2591142/150‑1 (MQ=255)
                                                          aCGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGc                                                                                        >  1:8098064/1‑150 (MQ=255)
                                                             gtgtCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACCGCGCGGTTATCAGTTCCCGCCGTGGCTgc                                                                                     >  2:21138/1‑150 (MQ=255)
                                                               gtCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTgcgc                                                                                   <  2:10598538/150‑1 (MQ=255)
                                                                tCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCt                                                                                  >  2:1343058/1‑150 (MQ=255)
                                                                 cGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTg                                                                                 >  2:2413366/1‑150 (MQ=255)
                                                                  ggTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTgg                                                                                <  2:558863/150‑1 (MQ=255)
                                                                   gTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCTCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGc                                                                               <  2:8278041/150‑1 (MQ=255)
                                                                     gccggccgCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCa                                                                             >  1:9276183/1‑150 (MQ=255)
                                                                       cggccgCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCAcc                                                                           >  2:9720810/1‑150 (MQ=255)
                                                                        ggccgCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCt                                                                          <  2:1861311/150‑1 (MQ=255)
                                                                         gccgccTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCtt                                                                         >  1:5135798/1‑150 (MQ=255)
                                                                         gccgccTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCtt                                                                         >  1:10534185/1‑150 (MQ=255)
                                                                         gccgccTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCtt                                                                         >  1:2924108/1‑150 (MQ=255)
                                                                          ccgccTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCttt                                                                        >  1:1721991/1‑150 (MQ=255)
                                                                           cgccTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTc                                                                       <  2:10076700/150‑1 (MQ=255)
                                                                            gccTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCt                                                                      >  1:66325/1‑150 (MQ=255)
                                                                            gccTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCt                                                                      >  2:4394403/1‑150 (MQ=255)
                                                                             ccTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCtt                                                                     >  2:6074183/1‑150 (MQ=255)
                                                                              cTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTg                                                                    >  1:1801244/1‑150 (MQ=255)
                                                                              cTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTg                                                                    >  1:5297547/1‑150 (MQ=255)
                                                                               tGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGc                                                                   <  2:3343196/150‑1 (MQ=255)
                                                                                   tgcgctgcTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGc                                                               >  1:3732864/1‑150 (MQ=255)
                                                                                    gcgctgcTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGcc                                                              >  2:8572099/1‑150 (MQ=255)
                                                                                    gcgctgcTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGcc                                                              >  2:3137/1‑150 (MQ=255)
                                                                                         gctCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGa                                                         <  2:884275/150‑1 (MQ=255)
                                                                                          ctCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAg                                                        >  2:9383371/1‑150 (MQ=255)
                                                                                          ctCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAg                                                        <  2:888258/150‑1 (MQ=255)
                                                                                             gttgttCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGcc                                                     <  2:7158218/150‑1 (MQ=255)
                                                                                              ttgttCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGccc                                                    <  2:4747573/150‑1 (MQ=255)
                                                                                              ttgttCTGCTCGCTCATCGGGTTCCTCTCCTCATCCTGGGCATGAACATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGccc                                                    <  1:6809432/150‑1 (MQ=255)
                                                                                                  tCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTg                                                >  1:2216562/1‑150 (MQ=255)
                                                                                                      ctcgctcATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGa                                            <  1:8581707/150‑1 (MQ=255)
                                                                                                       tcgctcATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGAt                                           >  1:2655375/1‑150 (MQ=255)
                                                                                                       tcgctcATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGAt                                           <  2:3848664/150‑1 (MQ=255)
                                                                                                            cATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTc                                      >  1:187014/1‑150 (MQ=255)
                                                                                                             aTCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCt                                     <  2:2454/150‑1 (MQ=255)
                                                                                                                ggTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCTTGa                                  <  2:5919646/150‑1 (MQ=255)
                                                                                                                 gTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCTTGaa                                 >  1:127332/1‑150 (MQ=255)
                                                                                                                   ttCCTCTCCTCATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCTTGAAGt                               <  1:7637895/150‑1 (MQ=255)
                                                                                                                    tcctctcctcATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCTTGAAGtt                              >  1:5568560/1‑150 (MQ=255)
                                                                                                                     cctctcctcATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCTTGAAGTTg                             <  2:3711866/150‑1 (MQ=255)
                                                                                                                     cctctcctcATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCTTGAAGTTg                             <  2:3703309/150‑1 (MQ=255)
                                                                                                                     cctctcctcATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCTTGAAGTTg                             >  1:4982892/1‑150 (MQ=255)
                                                                                                                       tctcctcATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCTTGAAGTTgcc                           >  1:411553/1‑150 (MQ=255)
                                                                                                                         tcctcATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCTTGAAGTTgccgc                         >  1:10069989/1‑150 (MQ=255)
                                                                                                                             cATCCTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCTTGAAGTTGCCGCCGct                     <  1:4741967/150‑1 (MQ=255)
                                                                                                                                 cTGGGCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCTTGAAGTTGCCGCCGctggcc                 >  1:9253216/1‑150 (MQ=255)
                                                                                                                                     gCATGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCTTGAAGTTGCCGCCGctggcctggc             <  1:141229/150‑1 (MQ=255)
                                                                                                                                       aTGAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCTTGAAGTTGCCGCCGCTGGCCTGgccg           >  2:4027473/1‑150 (MQ=255)
                                                                                                                                        ngAAAATGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCTTGAAGTTGCCGCCGCTGGCCTGgccgc          >  1:2094511/2‑150 (MQ=255)
                                                                                                                                            aaTGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCTTGAAGTTGCCGCCGCTGGCCTGGCCGCCctt      >  1:7128128/1‑150 (MQ=255)
                                                                                                                                            aaTGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCTTGAAGTTGCCGCCGCTGGCCTGGCCGCCctt      >  2:10311402/1‑150 (MQ=255)
                                                                                                                                             aTGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCTTGAAGTTGCCGCCGCTGGCCTGGCCGCCcttc     >  2:7430367/1‑150 (MQ=255)
                                                                                                                                             aTGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCTTGAAGTTGCCGCCGCTGGCCTGGCCGCCcttc     >  2:5820227/1‑150 (MQ=255)
                                                                                                                                             aTGGGGCGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCTTGAAGTTGCCGCCGCTGGCCTGGCCGCCcttc     >  2:3028546/1‑150 (MQ=255)
                                                                                                                                               ggggcggATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCTTGAAGTTGCCGCCGCTGGCCTGGCCGCCcttctt   >  2:7932650/1‑150 (MQ=255)
                                                                                                                                                gggcggATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCTTGAAGTTGCCGCCGCTGGCCTGGCCGCCCTTCTTg  <  2:1048387/150‑1 (MQ=255)
                                                                                                                                                gggcggATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCTTGAAGTTGCCGCCGCTGGCCTGGCCGCCCTTCTTg  >  1:8985673/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGCGTCCTCGCGATAGGCCTCCAGCTGTTCCAGCTTGGCGTTGCTGTTGCCACGGTCGACGGTGTCGGTGCCGGCCGCCTGGCTGCGCTGCTCGTTGTTCTGCTCGCTCATCGGTTTCCTCTCCTCATCCTGGGCATGAAAATGGGGGGGATGCCTCCCTGCTCCGCCCCATTCACTTCACTGCGCGGTTATCAGTTCCCGCCGTGGCTGCGCTGGCCACCTTTCTTGCCAGCCTCGGAGGCCCGCTGCGGATCGTTCTTGAAGTTGCCGCCGCTGGCCTGGCCGCCCTTCTTG  >  NC_008463/3281330‑3281623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑