Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,670,384 A→G Q128Q (CAA→CAG)  PA14_RS16675 → ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,670,3840AG99.8% 1522.7 / NA 394Q128Q (CAA→CAG) PA14_RS16675ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (190/202);  minor base T (1/0);  total (191/203)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 394 total aligned reads displayed.

CCCCTACATCAGCCAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAAGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCA  >  NC_008463/3670237‑3670530
                                                                                                                                                   |                                                                                                                                                  
  ccTACATCAGCCAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGccc                                                                                                                                                >  2:2245385/1‑150 (MQ=255)
    tACATCAGCCAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCcgc                                                                                                                                              <  1:6948801/150‑1 (MQ=255)
    tACATCAGCCAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCcgc                                                                                                                                              <  1:4485444/150‑1 (MQ=255)
     aCATCAGCCAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCcgcg                                                                                                                                             >  2:1014871/1‑150 (MQ=255)
       aTCAGCCAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGATGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGAc                                                                                                                                           >  2:5223935/1‑150 (MQ=255)
              aaCTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCg                                                                                                                                    >  2:8048551/1‑150 (MQ=255)
                  gATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCCGGCCCGCGACGAGCTCGCCCg                                                                                                                                >  2:6823680/1‑150 (MQ=255)
                  gATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCg                                                                                                                                >  1:5061562/1‑150 (MQ=255)
                   aTCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCgg                                                                                                                               <  1:5226692/150‑1 (MQ=255)
                   aTCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCgg                                                                                                                               <  1:10278451/150‑1 (MQ=255)
                    tCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCggg                                                                                                                              <  2:5828444/150‑1 (MQ=255)
                      gCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTg                                                                                                                            >  1:1077121/1‑150 (MQ=255)
                           gaAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTa                                                                                                                       <  1:3539055/150‑1 (MQ=255)
                           gaAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTa                                                                                                                       >  2:3102511/1‑150 (MQ=255)
                            aaGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTAc                                                                                                                      >  2:8973003/1‑150 (MQ=255)
                            aaGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTCCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTAc                                                                                                                      >  1:8092030/1‑150 (MQ=255)
                               gACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGc                                                                                                                   <  1:7217941/150‑1 (MQ=255)
                                ccggcggGGTGCTTTGGCCGCCGCTCCCCTCCAGCGACGACACCCTCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCt                                                                                                                  <  2:4858837/149‑1 (MQ=37)
                                 cGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCtt                                                                                                                 <  1:7421637/150‑1 (MQ=255)
                                  ggCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTc                                                                                                                >  1:8273690/1‑150 (MQ=255)
                                   gCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTcc                                                                                                               >  2:84721/1‑150 (MQ=255)
                                   gCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTcc                                                                                                               >  1:1653447/1‑150 (MQ=255)
                                   gCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTcc                                                                                                               >  1:9563170/1‑150 (MQ=255)
                                    cTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCcg                                                                                                              >  2:3562552/1‑150 (MQ=255)
                                       ggTGCTTTGGCCGCCGATCCCCTCCAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCgt                                                                                                           <  1:4828705/150‑1 (MQ=255)
                                       ggTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCgt                                                                                                           <  2:1357906/150‑1 (MQ=255)
                                       ggTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCgt                                                                                                           <  2:9280109/150‑1 (MQ=255)
                                         tGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCgtgt                                                                                                         <  2:6312106/150‑1 (MQ=255)
                                         tGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCgtgt                                                                                                         >  2:3817641/1‑150 (MQ=255)
                                            tttGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGa                                                                                                      <  1:6780391/150‑1 (MQ=255)
                                                 ccgccgATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCctc                                                                                                 <  2:9520016/150‑1 (MQ=255)
                                                 ccgccgATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCctc                                                                                                 <  1:1749115/150‑1 (MQ=255)
                                                 ccgccgATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCctc                                                                                                 <  1:7730414/150‑1 (MQ=255)
                                                 ccgccgATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCctc                                                                                                 <  1:3179374/150‑1 (MQ=255)
                                                   gccgATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCtt                                                                                               <  1:9240113/150‑1 (MQ=255)
                                                   gccgATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCtt                                                                                               <  2:7000547/150‑1 (MQ=255)
                                                   gccgATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCtt                                                                                               >  2:7439675/1‑150 (MQ=255)
                                                   gccgATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTCCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCtt                                                                                               <  2:7201949/150‑1 (MQ=255)
                                                      gATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGc                                                                                            >  1:7082600/1‑150 (MQ=255)
                                                       aTCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGcc                                                                                           >  1:1952133/1‑150 (MQ=255)
                                                       aTCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGcc                                                                                           <  1:9960856/150‑1 (MQ=255)
                                                       aTCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATGTACGGCTTCCGCGTGTCGATCCTCTTCGcc                                                                                           >  2:9354548/1‑150 (MQ=255)
                                                        tCCCCTACCGCTACGACACCCTCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGccc                                                                                          <  2:3259774/150‑1 (MQ=255)
                                                        tCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGccc                                                                                          <  1:10168703/150‑1 (MQ=255)
                                                        tCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGccc                                                                                          >  2:1470986/1‑150 (MQ=255)
                                                        tCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGccc                                                                                          >  2:3441309/1‑150 (MQ=255)
                                                         ccccTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGccct                                                                                         <  2:7683031/150‑1 (MQ=255)
                                                         ccccTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGccct                                                                                         >  1:2434104/1‑150 (MQ=255)
                                                           ccTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGccctga                                                                                       >  2:628252/1‑150 (MQ=255)
                                                            cTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGccctgac                                                                                      >  1:2982488/1‑150 (MQ=255)
                                                             tACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGccctgacc                                                                                     <  1:6384054/150‑1 (MQ=255)
                                                             tACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGccctgacc                                                                                     >  2:9329763/1‑150 (MQ=255)
                                                               cAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGccctgaccct                                                                                   <  1:9806232/150‑1 (MQ=255)
                                                                 gCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGccctgaccctga                                                                                 >  1:10032010/1‑150 (MQ=255)
                                                                 gCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGccctgaccctga                                                                                 <  1:2141150/150‑1 (MQ=255)
                                                                  cTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCcctgaccctgac                                                                                >  1:5381941/1‑150 (MQ=255)
                                                                    aCGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCg                                                                              >  2:10570703/1‑150 (MQ=255)
                                                                     cGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGt                                                                             <  1:5928880/150‑1 (MQ=255)
                                                                         acCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTc                                                                         <  2:1140795/150‑1 (MQ=255)
                                                                              cAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTtc                                                                    <  1:4376592/150‑1 (MQ=255)
                                                                                aCTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTtctc                                                                  >  1:4294812/1‑150 (MQ=255)
                                                                                 cTACGAGCTGAAGGGTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTctct                                                                 <  2:2626972/150‑1 (MQ=255)
                                                                                   aCGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGa                                                               <  2:7533054/150‑1 (MQ=255)
                                                                                    cGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGAt                                                              >  1:6464190/1‑150 (MQ=255)
                                                                                    cGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGAt                                                              <  2:5481710/150‑1 (MQ=255)
                                                                                    cGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCAGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGAt                                                              <  2:66527/150‑1 (MQ=255)
                                                                                       gCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCgg                                                           <  2:499857/150‑1 (MQ=255)
                                                                                            aGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCa                                                      <  1:9379463/150‑1 (MQ=255)
                                                                                             ggTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCAc                                                     >  1:3733373/1‑150 (MQ=255)
                                                                                              gTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCAcc                                                    >  2:4064464/1‑150 (MQ=255)
                                                                                              gTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCAcc                                                    <  2:7891772/150‑1 (MQ=255)
                                                                                                tCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGt                                                  <  1:9702639/150‑1 (MQ=255)
                                                                                                 cccgcccccgccCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTc                                                 <  2:8247543/150‑1 (MQ=255)
                                                                                                 cccgcccccgccCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTc                                                 >  2:188558/1‑150 (MQ=255)
                                                                                                 cccgcccccgccCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTc                                                 >  1:5618868/1‑150 (MQ=255)
                                                                                                  ccgcccccgcccCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCg                                                <  1:3066915/150‑1 (MQ=255)
                                                                                                     cccccgccccGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGgcg                                             >  1:3373991/1‑150 (MQ=255)
                                                                                                     cccccgccccGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGgcg                                             >  2:9261284/1‑150 (MQ=255)
                                                                                                        ccgccccgccGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGccc                                          >  2:4439450/1‑150 (MQ=255)
                                                                                                         cgccccgccGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCt                                         >  1:2223222/1‑150 (MQ=255)
                                                                                                           ccccgccGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGc                                       >  2:10447743/1‑150 (MQ=255)
                                                                                                            cccgccGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCa                                      >  1:6144105/1‑150 (MQ=255)
                                                                                                             ccgccgTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCaa                                     >  2:8736627/1‑150 (MQ=255)
                                                                                                                ccgTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGc                                  <  2:9860765/150‑1 (MQ=255)
                                                                                                                ccgTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGc                                  >  1:5141147/1‑150 (MQ=255)
                                                                                                                     ggcggAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGctacta                             >  1:6720492/1‑150 (MQ=255)
                                                                                                                          aaaaCTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTAcggcg                        <  1:7913517/150‑1 (MQ=255)
                                                                                                                           aaaCTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTAcggcgg                       >  1:1361028/1‑150 (MQ=255)
                                                                                                                            aaCTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACggcggc                      <  2:5614807/150‑1 (MQ=255)
                                                                                                                             aCTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCt                     >  2:2750965/1‑150 (MQ=255)
                                                                                                                              ctggctggGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTg                    >  2:6000020/1‑150 (MQ=255)
                                                                                                                                ggctggGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTggg                  <  2:1662029/150‑1 (MQ=255)
                                                                                                                                   tggGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGACCTGCAAGGCTACTACGGCGGCTGGGTCg               <  1:8836189/150‑1 (MQ=255)
                                                                                                                                    gggCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGa              >  2:3684427/1‑150 (MQ=255)
                                                                                                                                      gCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGAcc            <  1:2173675/150‑1 (MQ=255)
                                                                                                                                       cACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCt           <  1:8502603/150‑1 (MQ=255)
                                                                                                                                         ccGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGc         >  1:6609277/1‑150 (MQ=255)
                                                                                                                                            acgacCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCg      <  1:2691642/150‑1 (MQ=255)
                                                                                                                                            acgacCAGGCCCGCGACGTGCTCGCCCGGGTGAGTTACGGCTTCCGCGTGTCGATCCTCGTCGCCCAGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCACGGCTACTACGGCGGCTGGGTCGACCGGCTCg      >  2:4589576/1‑150 (MQ=255)
                                                                                                                                                ccAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCa  >  1:3937978/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CCCCTACATCAGCCAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAAGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCA  >  NC_008463/3670237‑3670530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑