Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,879,553 A→G F52L (TTC→CTC)  PA14_RS17695 ← LysE family translocator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,879,5530AG99.8% 1770.7 / NA 456F52L (TTC→CTC) PA14_RS17695LysE family translocator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (246/208);  minor base T (1/0);  total (247/209)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 460 total aligned reads displayed.

CGTCGCCGCGCCGCAGCCAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAAAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCAAGACAGACAATT  >  NC_008463/3879406‑3879699
                                                                                                                                                   |                                                                                                                                                  
cGTCGCCGCGCCGCAGCCAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTCTGCAGGagaa                                                                                                                                                  >  2:9860421/1‑150 (MQ=255)
  tCGCCGCGCCGCAGCCAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATc                                                                                                                                                <  2:6339273/150‑1 (MQ=255)
   cgccgcgccgCAGCCAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATcc                                                                                                                                               <  1:6280282/150‑1 (MQ=255)
   cgccgcgccgCAGCCAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATcc                                                                                                                                               <  1:3470380/150‑1 (MQ=255)
    gccgcgccgcAGCCAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATccc                                                                                                                                              >  2:337438/1‑150 (MQ=255)
      cgcgccgcAGCCAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGc                                                                                                                                            <  1:9189873/150‑1 (MQ=255)
         gccgcAGCCAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAgg                                                                                                                                         <  1:5427323/150‑1 (MQ=255)
          ccgcAGCCAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGCGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGc                                                                                                                                        >  2:4280210/1‑150 (MQ=255)
           cgcAGCCAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGcc                                                                                                                                       <  1:10257547/150‑1 (MQ=255)
                ccAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGccc                                                                                                                                  >  2:10445863/1‑150 (MQ=255)
                 cAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGcccc                                                                                                                                 <  1:2170841/150‑1 (MQ=255)
                 cAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGcccc                                                                                                                                 <  1:5236540/150‑1 (MQ=255)
                    gcgcgcAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAgc                                                                                                                              <  1:6829390/150‑1 (MQ=255)
                      gcgcAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACACGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGACTCCCGCAGGCCAGCCCCAgccc                                                                                                                            >  1:6866643/1‑148 (MQ=255)
                         cAGCGCTCCGACGCCGCGCCAGGCCAGGTACAGCGCCCCGAGCGCCTGCAGGATGGCGACCAGCACAGGCGTACGGCTGCGCAGCAGCGACACGCCGGTCCGCACCAGGGTCGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCt                                                                                                                         <  2:1663355/150‑1 (MQ=255)
                         cAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCt                                                                                                                         <  2:876909/150‑1 (MQ=255)
                          aGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCCGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTg                                                                                                                        >  1:7738880/1‑150 (MQ=255)
                           gcgcgcCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTgg                                                                                                                       >  1:1443088/1‑150 (MQ=255)
                             gcgcCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGcc                                                                                                                     <  2:4851761/150‑1 (MQ=255)
                             gcgcCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGcc                                                                                                                     >  1:4127238/1‑150 (MQ=255)
                                ccgacgccgaGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAgg                                                                                                                  >  1:994097/1‑150 (MQ=255)
                                ccgacgccgaGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAgg                                                                                                                  <  1:8822702/150‑1 (MQ=255)
                                  gacgccgaGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAggcc                                                                                                                >  1:5616989/1‑150 (MQ=255)
                                   acgccgaGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAggccg                                                                                                               <  1:6157820/150‑1 (MQ=255)
                                       cgaGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGccc                                                                                                           >  1:3875997/1‑150 (MQ=255)
                                        gaGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCccg                                                                                                          >  1:4211860/1‑150 (MQ=255)
                                         aGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCCGCGCGCTGGCCAGGACGGCccgc                                                                                                         >  2:8312963/1‑150 (MQ=255)
                                         aGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCccgc                                                                                                         >  2:4398663/1‑150 (MQ=255)
                                                    gTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGTATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTg                                                                                              >  1:4445077/1‑150 (MQ=255)
                                                       cAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAg                                                                                           <  2:8004796/150‑1 (MQ=255)
                                                        aGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAgg                                                                                          <  1:1137137/150‑1 (MQ=255)
                                                            agcagAGCGACTGCATGATGGCGCACAGCACAGGCGTACGGCTTAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCATGAGAATCCCGCAGTCCAGCCCCATAGCGCTGGCCAGGCCTGCCCGCCGCCCCTGGTGCAGGGACg                                                                                      <  1:758016/146‑1 (MQ=255)
                                                             gcCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGt                                                                                     >  1:598434/1‑150 (MQ=255)
                                                             gcCGAGCGCCTGCAGGAGGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGt                                                                                     <  2:7521796/150‑1 (MQ=255)
                                                                gAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGCCGTgcg                                                                                  >  2:6863848/1‑150 (MQ=255)
                                                                gAGCGCCTGCAGGAGGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTgcg                                                                                  <  1:8580956/150‑1 (MQ=255)
                                                                        gCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCAccacca                                                                          >  1:6651863/1‑150 (MQ=255)
                                                                           ggATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAggg                                                                       >  1:7515159/1‑150 (MQ=255)
                                                                            gATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGc                                                                      >  2:3820077/1‑150 (MQ=255)
                                                                               ggCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGAc                                                                   >  1:2549835/1‑150 (MQ=255)
                                                                                  gAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATc                                                                >  1:3975363/1‑150 (MQ=255)
                                                                                     cAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCggg                                                             >  1:5779077/1‑150 (MQ=255)
                                                                                     cAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCggg                                                             >  1:4070483/1‑150 (MQ=255)
                                                                                             gCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGAGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGc                                                     <  2:333631/150‑1 (MQ=255)
                                                                                              cGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGACTCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCt                                                    <  2:10597700/150‑1 (MQ=255)
                                                                                              cGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCt                                                    <  1:3505591/150‑1 (MQ=255)
                                                                                               gTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTg                                                   >  2:4213317/1‑150 (MQ=255)
                                                                                               gTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTg                                                   >  1:4984191/1‑150 (MQ=255)
                                                                                               gTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTg                                                   >  2:8245845/1‑150 (MQ=255)
                                                                                                    gCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAg                                              >  2:5609128/1‑150 (MQ=255)
                                                                                                    gCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAg                                              >  2:2777360/1‑150 (MQ=255)
                                                                                                    gCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAg                                              >  2:2117218/1‑150 (MQ=255)
                                                                                                    gCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAg                                              <  1:338267/150‑1 (MQ=255)
                                                                                                     cTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAgg                                             <  1:10051325/150‑1 (MQ=255)
                                                                                                      tGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGGATGCTGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCCGGGACGGGCGCACCACCGGGGCGACATCGGGTCCGGGGCTGACGAggg                                            >  2:1519492/1‑150 (MQ=255)
                                                                                                       gAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGc                                           >  2:9627569/1‑150 (MQ=255)
                                                                                                       gAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGc                                           >  1:10720421/1‑150 (MQ=255)
                                                                                                       gAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAAGCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCGGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGc                                           >  2:3147539/1‑150 (MQ=255)
                                                                                                         gccgcagcGACACGCCGGTCCGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGa                                         <  2:7783628/147‑1 (MQ=255)
                                                                                                         gcagcagcGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGa                                         <  1:7842130/150‑1 (MQ=255)
                                                                                                          cagcagcGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCCGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGAc                                        <  2:2497314/150‑1 (MQ=255)
                                                                                                          cagcagcGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGAc                                        >  1:7991179/1‑150 (MQ=255)
                                                                                                            gcagcGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGACTCCCGCAGGCCAGCCCCCGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACgg                                      <  1:3286023/150‑1 (MQ=255)
                                                                                                            gcagcGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACgg                                      >  1:1968593/1‑150 (MQ=255)
                                                                                                            gcagcGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACgg                                      >  2:8933063/1‑150 (MQ=255)
                                                                                                              agcGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCg                                    <  2:1843965/150‑1 (MQ=255)
                                                                                                               gcGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGa                                   >  2:2226684/1‑150 (MQ=255)
                                                                                                               gcGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGa                                   >  1:6588982/1‑150 (MQ=255)
                                                                                                                 gACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAac                                 <  1:9058373/150‑1 (MQ=255)
                                                                                                                  acacGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAaca                                >  2:6526114/1‑150 (MQ=255)
                                                                                                                   cacGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAacac                               >  1:8278520/1‑150 (MQ=255)
                                                                                                                    acGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACAcc                              <  2:8952153/150‑1 (MQ=255)
                                                                                                                      gCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACAccgc                            <  1:2891027/150‑1 (MQ=255)
                                                                                                                       ccGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGACCAccgcc                           >  2:6114469/1‑150 (MQ=255)
                                                                                                                          gTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCgccgcc                        >  1:2013361/1‑150 (MQ=255)
                                                                                                                          gTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCgccgcc                        >  2:4579665/1‑150 (MQ=255)
                                                                                                                            cAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAg                      >  1:3092109/1‑150 (MQ=255)
                                                                                                                            cAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCACGCCGGCCCGCCGCCCCTCGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAg                      <  1:7367528/150‑1 (MQ=255)
                                                                                                                             aGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAgg                     >  2:7191813/1‑150 (MQ=255)
                                                                                                                             aGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAgg                     <  1:3819751/150‑1 (MQ=255)
                                                                                                                             aGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAgg                     >  2:1121598/1‑150 (MQ=255)
                                                                                                                                aCCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCCGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTc                  >  2:5470620/1‑150 (MQ=255)
                                                                                                                                aCCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTc                  >  2:8201631/1‑150 (MQ=255)
                                                                                                                                  cAGGGTGGTATGCAGGAGAATCCCGCCGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAg                <  2:2977032/150‑1 (MQ=255)
                                                                                                                                   aGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGc               >  2:5393432/1‑150 (MQ=255)
                                                                                                                                   aGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGc               >  2:6304617/1‑150 (MQ=255)
                                                                                                                                   aGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGc               >  1:5112083/1‑150 (MQ=255)
                                                                                                                                    gggTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCCCCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCa              <  2:5124357/150‑1 (MQ=255)
                                                                                                                                     ggtggtATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCaa             <  2:5242355/150‑1 (MQ=255)
                                                                                                                                       tggtATGCAAGAGAATCCCGCAGGCCAGCCCAAGCGCGCTGGCCATGCCGGCACGCCGCCCCTGGTTCAGGGACGTGCGCACCACCAGGGCGACATCGTGACCGGGGCTGACCTGGGCGACGGCGAACACCGCCGCCAGGGTCAGCAaga           >  1:2472609/1‑150 (MQ=255)
                                                                                                                                        ggtATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCAagac          <  1:10627457/150‑1 (MQ=255)
                                                                                                                                         gtATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCAagaca         <  1:1775574/150‑1 (MQ=255)
                                                                                                                                          tATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCAagacag        <  1:7092982/150‑1 (MQ=255)
                                                                                                                                          tATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCAagacag        <  2:3962581/150‑1 (MQ=255)
                                                                                                                                           aTGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCAagacaga       <  2:9288643/150‑1 (MQ=255)
                                                                                                                                             gCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCAAgacagaca     >  1:9859299/1‑150 (MQ=255)
                                                                                                                                             gCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCAAgacagaca     >  1:9309205/1‑150 (MQ=255)
                                                                                                                                                ggAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCAAGACAGACAAtt  <  1:6136902/150‑1 (MQ=255)
                                                                                                                                                ggAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCAAGACAGACAAtt  <  2:3627219/150‑1 (MQ=255)
                                                                                                                                                ggAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCAAGACAGACAAtt  >  2:10174677/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGTCGCCGCGCCGCAGCCAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAAAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCAAGACAGACAATT  >  NC_008463/3879406‑3879699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑