Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 4,565,093 C→G G290R (GGC→CGC)  PA14_RS20885 ← anthranilate synthase component 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084634,565,0930CG99.8% 2468.0 / NA 630G290R (GGC→CGC) PA14_RS20885anthranilate synthase component 1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (321/308);  minor base T (0/1);  total (321/309)
Fisher's exact test for biased strand distribution p-value = 4.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 630 total aligned reads displayed.

CATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCCCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCAAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCCCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCACCTGGCGAGCAAAGCTCGCATCGTCCAGGTCGA  >  NC_008463/4564946‑4565239
                                                                                                                                                   |                                                                                                                                                  
cATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCa                                                                                                                                                  <  1:269921/150‑1 (MQ=255)
  tCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAgg                                                                                                                                                >  1:1328658/1‑150 (MQ=255)
  tCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCGGGGGTTGCGCAgg                                                                                                                                                >  1:79049/1‑150 (MQ=255)
    gcgTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAGGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGACGCGGTACGGGCTGGGGTTGCGCAGGca                                                                                                                                              >  2:2450136/1‑150 (MQ=255)
     cgTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGcac                                                                                                                                             <  1:2333340/150‑1 (MQ=255)
     cgTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGcac                                                                                                                                             >  2:158780/1‑150 (MQ=255)
       tCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGACGCGCAGAACAGGCAGAAGTCCCCCGAAACGAGGTAGAAGGGGTACGGGCTGGGGTTGCGCGGGCACaa                                                                                                                                           >  2:6103699/1‑150 (MQ=255)
        ccGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAAc                                                                                                                                          <  1:7166390/150‑1 (MQ=255)
        ccGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAAc                                                                                                                                          <  2:7967112/150‑1 (MQ=255)
          gcgcgGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTg                                                                                                                                        <  2:10612471/150‑1 (MQ=255)
           cgcgGGCGATTGCCGGCAATGGGGTAGATTTCCACCACGCGACTCTCCGCGTCGTACTTCAAATCCGACTCCGGCGAAGCGCCGAACAGGCAGAATTCCCCCGCAACGAGGAAGAAGCGGTAAGGGCTGGGGTTGCGCAGGCACAACTgg                                                                                                                                       >  2:9310669/1‑150 (MQ=255)
              gggCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGa                                                                                                                                    <  1:9681653/150‑1 (MQ=255)
              gggCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGa                                                                                                                                    >  1:1731231/1‑150 (MQ=255)
               ggCGAGTGCCGGCCATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGACGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCTCAACTGGCGAt                                                                                                                                   >  2:6923835/1‑150 (MQ=255)
                  gAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAgg                                                                                                                                <  1:10479830/150‑1 (MQ=255)
                  gAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAgg                                                                                                                                <  2:181065/150‑1 (MQ=255)
                   aGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGc                                                                                                                               <  2:7093494/150‑1 (MQ=255)
                    gTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGcc                                                                                                                              <  2:4174800/150‑1 (MQ=255)
                     ggCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGccc                                                                                                                             <  1:4615411/149‑1 (MQ=255)
                      gCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCg                                                                                                                            >  2:2277196/1‑150 (MQ=255)
                         ggCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCa                                                                                                                         >  1:5001012/1‑150 (MQ=255)
                            aaTGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAggg                                                                                                                      >  1:1234766/1‑150 (MQ=255)
                            aaTGGGGAAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGATGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAggg                                                                                                                      <  2:3800530/150‑1 (MQ=255)
                             aTGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAgggg                                                                                                                     <  2:10755109/150‑1 (MQ=255)
                               ggggTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTc                                                                                                                   >  1:5837596/1‑150 (MQ=255)
                                gggTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTcc                                                                                                                  >  2:8429644/1‑150 (MQ=255)
                                  gTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCcgc                                                                                                                <  2:5529224/150‑1 (MQ=255)
                                  gTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCcgc                                                                                                                <  2:10184120/150‑1 (MQ=255)
                                    agagTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCgcgc                                                                                                              >  1:8753909/1‑150 (MQ=255)
                                          ccACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCa                                                                                                        >  1:5113945/1‑150 (MQ=255)
                                           cACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCAt                                                                                                       <  2:5964984/150‑1 (MQ=255)
                                              cTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCt                                                                                                    >  2:5259469/1‑150 (MQ=255)
                                                  cgACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAg                                                                                                <  2:7393281/150‑1 (MQ=255)
                                                   gACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGc                                                                                               >  1:938710/1‑150 (MQ=255)
                                                    aCTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCt                                                                                              >  2:722017/1‑150 (MQ=255)
                                                    aCTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCt                                                                                              <  1:8040250/150‑1 (MQ=255)
                                                      tctcCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTgc                                                                                            <  1:6014178/150‑1 (MQ=255)
                                                      tctcCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTgc                                                                                            <  2:3317335/150‑1 (MQ=255)
                                                       ctcCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTgcg                                                                                           >  2:3681009/1‑150 (MQ=255)
                                                        tcCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTgcgc                                                                                          <  1:4267114/150‑1 (MQ=255)
                                                        tcCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTgcgc                                                                                          >  2:2913109/1‑150 (MQ=255)
                                                         ccgcgtcgtACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGcgcg                                                                                         >  1:9923188/4‑150 (MQ=255)
                                                         ccgcgtcgtACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGcgcg                                                                                         <  2:8087932/147‑1 (MQ=255)
                                                         ccgcgtcgtACTTCAATGCCGACTACGGCGACGCGCCGAACAGGCAGAAGTCCCCCGCATAGAGGAAGAAGCGGTACGGGCAGGGGTTGCGCAGGCACAACTGGCGATAGGACAGCCAGGGGTCCGCCCTCGGCATGCTGAAGCTgcgag                                                                                         >  2:9720274/4‑148 (MQ=255)
                                                          cgcgtcgtACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGa                                                                                        >  2:5852029/3‑150 (MQ=255)
                                                           gcgtcgtACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGAc                                                                                       >  2:2163023/2‑150 (MQ=255)
                                                           gcgtcgtACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGAc                                                                                       >  2:498384/2‑150 (MQ=255)
                                                                 tACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACg                                                                                 >  1:811466/1‑150 (MQ=255)
                                                                   cTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTCCGAt                                                                               >  2:2026688/1‑150 (MQ=255)
                                                                     tCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCt                                                                             >  2:9559554/1‑150 (MQ=255)
                                                                          gCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAac                                                                        <  2:2829358/150‑1 (MQ=255)
                                                                           ccGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAaca                                                                       <  1:2546692/150‑1 (MQ=255)
                                                                              aCTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGt                                                                    <  2:781388/150‑1 (MQ=255)
                                                                               cTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGACGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGAGCTGGAACACGTc                                                                   >  2:2603052/1‑150 (MQ=255)
                                                                               cTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTc                                                                   >  1:3695423/1‑150 (MQ=255)
                                                                                 ccGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGc                                                                 <  2:3173502/150‑1 (MQ=255)
                                                                                 ccGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGc                                                                 <  1:8464442/150‑1 (MQ=255)
                                                                                 ccGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGc                                                                 >  2:5623626/1‑150 (MQ=255)
                                                                                  cGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGcc                                                                <  1:47588/150‑1 (MQ=255)
                                                                                  cGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGcc                                                                <  1:6433890/150‑1 (MQ=255)
                                                                                   ggCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCg                                                               <  2:8516268/150‑1 (MQ=255)
                                                                                      gAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGcc                                                            <  2:680479/150‑1 (MQ=255)
                                                                                       aaGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGccc                                                           <  1:10079199/150‑1 (MQ=255)
                                                                                        aGCGCCGAACCGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGTCCCAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGCTTCGATCTGGAACACGTCGCCGGCCCt                                                          <  1:1482731/150‑1 (MQ=255)
                                                                                         gcgcCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTc                                                         <  1:6645520/150‑1 (MQ=255)
                                                                                         gcgcCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTc                                                         <  1:4471898/150‑1 (MQ=255)
                                                                                          cgcCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCa                                                        <  2:6612947/150‑1 (MQ=255)
                                                                                             cgaaCAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGt                                                     <  1:4909921/148‑1 (MQ=255)
                                                                                              gaaCAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGACGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTg                                                    >  2:9882783/2‑150 (MQ=255)
                                                                                              gaaCAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTg                                                    >  1:8353297/2‑150 (MQ=255)
                                                                                                aCAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTggg                                                  <  1:4106484/150‑1 (MQ=255)
                                                                                                  aGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGcc                                                <  2:9057671/150‑1 (MQ=255)
                                                                                                   ggCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCt                                               >  2:6333196/1‑150 (MQ=255)
                                                                                                        aaGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAgg                                          <  2:4836495/150‑1 (MQ=255)
                                                                                                        aaGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAgg                                          >  1:6270346/1‑150 (MQ=255)
                                                                                                        aaGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAgg                                          >  2:4550318/1‑150 (MQ=255)
                                                                                                         aGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGgc                                         >  2:7898009/1‑150 (MQ=255)
                                                                                                            cccccGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCt                                      >  2:3565983/1‑150 (MQ=255)
                                                                                                             ccccGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTc                                     >  2:7651287/1‑150 (MQ=255)
                                                                                                             ccccGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTc                                     <  1:3870060/150‑1 (MQ=255)
                                                                                                               ccGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCa                                   >  1:7643191/1‑150 (MQ=255)
                                                                                                               ccGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCa                                   <  1:4333580/150‑1 (MQ=255)
                                                                                                                cGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCAc                                  <  2:111955/150‑1 (MQ=255)
                                                                                                                 gCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGAACTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCAcc                                 <  2:4411751/150‑1 (MQ=255)
                                                                                                                  cATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCACCt                                >  1:10767252/1‑150 (MQ=255)
                                                                                                                   aTCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGCTAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTCCGATCTGGACCACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCACCTg                               >  2:6484031/1‑150 (MQ=255)
                                                                                                                   aTCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCACCTg                               >  1:10654352/1‑150 (MQ=255)
                                                                                                                    tCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCACCTgg                              <  1:5453870/150‑1 (MQ=255)
                                                                                                                          aagaagCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCACCTGGCGAGCa                        <  1:1168803/150‑1 (MQ=255)
                                                                                                                            gaagCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCACCTGGCGAGCaaa                      <  2:8878255/150‑1 (MQ=255)
                                                                                                                             aagCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCACCTGGCGAGCAAAg                     >  2:1032683/1‑150 (MQ=255)
                                                                                                                                    aCGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCACCTGGCGAGCAAAGCTCGCAt              <  2:7290423/150‑1 (MQ=255)
                                                                                                                                    aCGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCACCTGGCGAGCAAAGCTCGCAt              <  1:286041/150‑1 (MQ=255)
                                                                                                                                      gggCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCACCTGGCGAGCAAAGCTCGCATCg            <  1:3150351/150‑1 (MQ=255)
                                                                                                                                       ggCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCACCTGGCGAGCAAAGCTCGCATCGt           >  1:2082841/1‑150 (MQ=255)
                                                                                                                                          tGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGACCACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCACCTGGCGAGCAAAGCTCGCATCGTCCa        <  1:7332356/150‑1 (MQ=255)
                                                                                                                                             ggTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCACCTGGCGAGCAAAGCTCGCATCGTCCAGGt     >  1:8983346/1‑150 (MQ=255)
                                                                                                                                             ggTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCACCTGGCGAGCAAAGCTCGCATCGTCCAGGt     <  2:1259266/150‑1 (MQ=255)
                                                                                                                                             ggTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCACCTGGCGAGCAAAGCTCGCATCGTCCAGGt     >  1:3470853/1‑150 (MQ=255)
                                                                                                                                               ttGCTCAGGCACAACTGGCTATAGGCCCGCCAGGGGTCCGCGCACGGCATGATGAAGCTGCGCGACGTTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCACCTGGCGAGCAAAGCTCGCATCGTCCAGGTCg   <  1:5261234/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCCCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCAAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCCCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCACCTGGCGAGCAAAGCTCGCATCGTCCAGGTCGA  >  NC_008463/4564946‑4565239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑