Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 4,659,805 A→G intergenic (+45/+54) alpR → / ← PA14_RS21395 transcriptional regulator AlpR/tRNA‑Arg

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084634,659,8050AG100.0% 1505.8 / NA 383intergenic (+45/+54)alpR/PA14_RS21395transcriptional regulator AlpR/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (140/243);  total (140/243)

Only 100 of 394 total aligned reads displayed.

TTCAACAGCGACGAGCACCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAAAAAAATCCCGGGCAAAAAAAAAGGGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGTCGGTGCTTTTTCC  >  NC_008463/4659659‑4659952
                                                                                                                                                  |                                                                                                                                                   
ttCAACAGCGACGAGCACCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCACCGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCGTCCCGCCCCGCCTGACGCCGGGGCCGCGTTCCGGCGGaagaaa                                                                                                                                                  <  1:3238025/150‑1 (MQ=255)
        cgacgaGCACCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCgg                                                                                                                                          <  1:657884/150‑1 (MQ=255)
        cgacgaGCACCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCgg                                                                                                                                          <  1:3142162/150‑1 (MQ=255)
         gacgaGCACCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCggg                                                                                                                                         <  1:3959759/150‑1 (MQ=255)
         gacgaGCACCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCggg                                                                                                                                         <  2:7541053/150‑1 (MQ=255)
            gaGCACCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCaa                                                                                                                                      <  1:6928289/150‑1 (MQ=255)
             aGCACCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCaaa                                                                                                                                     >  1:4462387/1‑150 (MQ=255)
             aGCACCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCaaa                                                                                                                                     >  2:8866676/1‑150 (MQ=255)
              gCACCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCaaag                                                                                                                                    <  1:2474792/150‑2 (MQ=255)
               cACCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCaaaga                                                                                                                                   <  2:474262/150‑3 (MQ=255)
                  ccGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCaaagaaaa                                                                                                                                >  2:7957897/1‑150 (MQ=255)
                   cGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAAGCCCGGGCaaagaaaat                                                                                                                               >  2:7002361/1‑149 (MQ=255)
                    ggACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAAg                                                                                                                              <  2:3484287/150‑1 (MQ=255)
                     gACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAAgg                                                                                                                             >  1:2331979/1‑150 (MQ=255)
                       cGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAAgggc                                                                                                                           >  2:6372546/1‑149 (MQ=255)
                       cGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAAgggc                                                                                                                           <  1:732269/150‑2 (MQ=255)
                        gAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAACTCCCGGGCAAAGAAAAAgggcc                                                                                                                          >  2:8125471/1‑148 (MQ=255)
                        gAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAAgggcc                                                                                                                          <  1:7155660/150‑3 (MQ=255)
                         aGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCGTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAAgggcct                                                                                                                         <  1:6123/150‑4 (MQ=255)
                          gcgcTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAAgggcctg                                                                                                                        <  1:6791110/150‑5 (MQ=255)
                           cgcTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGc                                                                                                                      <  1:272489/150‑1 (MQ=255)
                           cgcTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGc                                                                                                                      <  2:5538818/150‑1 (MQ=255)
                            gcgaCGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGAACGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCGTCCCGCCCCGCCTGCCGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGCGCAAAGAACAA‑GGGCCTGCa                                                                                                                     <  2:6197885/147‑1 (MQ=255)
                            gcTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCa                                                                                                                     <  1:7547842/150‑1 (MQ=255)
                             cTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGC‑AAAGAAAATGGGCCTGCAt                                                                                                                    >  1:974100/1‑150 (MQ=255)
                              tACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCAtt                                                                                                                   >  1:4126536/1‑150 (MQ=255)
                              tACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCAtt                                                                                                                   <  1:1184592/150‑1 (MQ=255)
                               aCGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATtg                                                                                                                  >  1:3349529/1‑150 (MQ=255)
                                cGAGCCCCAGGAATCCGCCGCGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGACAAA‑GGGCCTGCATtgc                                                                                                                 <  2:10465969/150‑1 (MQ=255)
                                cGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATtgc                                                                                                                 <  2:8691980/150‑1 (MQ=255)
                                      cccggAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAgg                                                                                                           <  2:5829401/147‑1 (MQ=255)
                                      ccAGGACGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTCCTCGGTGCTGGTCTGCCCCCGGCCCGCCCCGCCTGCCGACGGGGCCGCGTTCCTGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAgg                                                                                                           <  2:9920714/150‑1 (MQ=255)
                                      ccAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAgg                                                                                                           >  2:6666450/1‑150 (MQ=255)
                                       cAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGc                                                                                                          <  1:3898851/150‑1 (MQ=255)
                                         ggAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGccc                                                                                                        <  1:8081425/150‑1 (MQ=255)
                                         ggAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGccc                                                                                                        >  2:7172286/1‑150 (MQ=255)
                                          gAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCt                                                                                                       <  1:2933744/150‑1 (MQ=255)
                                           acgccgccGAGCACATCAACGGGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCtt                                                                                                      <  2:1987402/148‑1 (MQ=255)
                                           aaGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCtt                                                                                                      <  1:8922614/150‑1 (MQ=255)
                                            aGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCttt                                                                                                     <  1:8320671/150‑1 (MQ=255)
                                               cgccgAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCgg                                                                                                  <  1:842054/150‑1 (MQ=255)
                                                 ccgAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTa                                                                                                <  1:1055491/150‑1 (MQ=255)
                                                   gAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATg                                                                                              >  2:1464254/1‑150 (MQ=255)
                                                       acaTCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGgcgc                                                                                          >  2:4613197/1‑150 (MQ=255)
                                                       acaTCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCGTCCCGCCCCGCCTGACGACGGGGCCGCGGTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGgcgc                                                                                          <  2:3898940/150‑1 (MQ=255)
                                                            aaCGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCa                                                                                     >  1:8032562/1‑150 (MQ=255)
                                                            aaCGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCa                                                                                     <  1:1740465/150‑1 (MQ=255)
                                                             aCGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCag                                                                                    <  1:171092/150‑1 (MQ=255)
                                                               gTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCagga                                                                                  <  1:7783305/150‑1 (MQ=255)
                                                                  aTCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCAGTGCTGCAGGCCCTTTCGGTATGGCGCACTCAggagga                                                                               <  2:9218662/150‑1 (MQ=255)
                                                                   tCGGCCGGGTGGTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGTGTCCGCTTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGAt                                                                              <  1:10059903/150‑1 (MQ=255)
                                                                      gCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTGTCGGTATGGCGCACTCAGGAGGATTCg                                                                           >  1:4311093/1‑150 (MQ=255)
                                                                       ccGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGa                                                                          >  2:10642922/1‑150 (MQ=255)
                                                                          ggTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAACATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAAcc                                                                       <  2:2694942/150‑1 (MQ=255)
                                                                             gtTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTcc                                                                    <  2:680828/150‑1 (MQ=255)
                                                                              ttCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCg                                                                   <  1:5220591/150‑1 (MQ=255)
                                                                                cTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGAc                                                                 <  2:8312767/150‑1 (MQ=255)
                                                                                   gTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGc                                                              <  1:7172286/150‑1 (MQ=255)
                                                                                     aCTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGGTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTc                                                            <  2:1634001/150‑1 (MQ=255)
                                                                                      cTCGGTGCTGGTCTGACCCCGACCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCg                                                           <  1:5380898/150‑1 (MQ=39)
                                                                                         ggTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGtt                                                        <  1:10330079/150‑1 (MQ=255)
                                                                                          gTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTc                                                       <  2:9274856/150‑1 (MQ=255)
                                                                                          gTGCTGGACTGACCCCGTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAACATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTc                                                       <  2:7388057/150‑1 (MQ=38)
                                                                                           tGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCg                                                      <  2:4134070/150‑1 (MQ=255)
                                                                                           tGCTGGTCTGACCCCGTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCg                                                      <  1:4775072/150‑1 (MQ=255)
                                                                                              tGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAg                                                   >  1:9444615/1‑150 (MQ=255)
                                                                                               ggTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGC‑AAAGAAATTGGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGc                                                  >  2:9770607/1‑150 (MQ=38)
                                                                                                 tCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGC‑AAAGAAAATGGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCg                                                >  1:2553689/1‑150 (MQ=255)
                                                                                                  cTGACCCCGTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGa                                               <  1:8799189/150‑1 (MQ=255)
                                                                                                      ccccTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTAc                                           >  1:4791572/1‑150 (MQ=255)
                                                                                                      ccccTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTAc                                           >  2:4286743/1‑150 (MQ=255)
                                                                                                       cccTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTAct                                          >  1:2153106/1‑150 (MQ=255)
                                                                                                          gtCCCGCCCCGCCTGACGACGGGGCCGCGTACCGGCGGAAGACAAATCCCGGGCTAAGAAATA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTTTGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTa                                       <  2:1305697/149‑1 (MQ=25)
                                                                                                          ctCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGACACA‑GGGCCGGCATTGCTGCAGGCCCTATCGGTATGGCGCACTCAGGAGGATTCGAACCGCCGACCGCTCGGTTCGTAGCCGAGTACTCTa                                       <  2:10199556/149‑1 (MQ=25)
                                                                                                            cccgccccgcCTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATc                                     <  1:3552165/150‑1 (MQ=255)
                                                                                                             ccgccccgccTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATcc                                    >  2:2407506/1‑150 (MQ=255)
                                                                                                                ccccgccTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGc                                 <  2:8446189/150‑1 (MQ=255)
                                                                                                                ccccgccTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGc                                 <  2:3391825/150‑1 (MQ=255)
                                                                                                                 cccgccTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCt                                <  1:8361956/150‑1 (MQ=255)
                                                                                                                  ccgccTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTg                               >  1:9017400/1‑150 (MQ=255)
                                                                                                                     ccTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGc                            <  1:1332678/150‑1 (MQ=255)
                                                                                                                      cTGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCt                           <  1:9628450/150‑1 (MQ=255)
                                                                                                                       tGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTa                          <  1:781167/150‑1 (MQ=255)
                                                                                                                       tGACGACGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTa                          >  2:5322518/1‑150 (MQ=255)
                                                                                                                        gacgacGGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTAt                         >  1:561920/1‑150 (MQ=255)
                                                                                                                           gacgGGGCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAg                      <  2:3216608/150‑1 (MQ=255)
                                                                                                                              ggggCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGc                   >  1:10758117/1‑150 (MQ=255)
                                                                                                                                ggCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCgt                 <  2:912003/150‑1 (MQ=255)
                                                                                                                                 gCCGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCgtg                <  1:6372546/150‑1 (MQ=255)
                                                                                                                                  ccGCGTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCgtgt               <  1:10627473/150‑1 (MQ=255)
                                                                                                                                     cgTTCCGGCGGAAGAAAAATCCCGCGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTAAGAGTGCGTGTCgg            <  2:1138321/150‑1 (MQ=21)
                                                                                                                                      gTTCCGGCGGAAGAAACAGCCCGGGCAAAGAAACA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCACGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGATTACTCTATCCAGCTGAGCTATGAGTGCGTGTCGGt           <  2:4967981/150‑1 (MQ=21)
                                                                                                                                      gTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGTCGGt           <  2:3028150/150‑1 (MQ=255)
                                                                                                                                      gTTCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGGATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGTCGGt           >  1:7314742/1‑150 (MQ=255)
                                                                                                                                        tCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGTCGGTGc         <  2:7668015/150‑1 (MQ=255)
                                                                                                                                        tCCGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGTCGGTGc         >  1:1524478/1‑150 (MQ=255)
                                                                                                                                         ccGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGTCGGTGCt        <  1:5715709/150‑1 (MQ=255)
                                                                                                                                         ccGGCGGAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGTCGGTGCt        <  2:2138241/150‑1 (MQ=255)
                                                                                                                                            gcggAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGTCGGTGCtttt     >  2:9516647/1‑150 (MQ=255)
                                                                                                                                            gcggAAGAAAAATCCCGGGCAAAGAAAAA‑GGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGTCGGTGCtttt     <  1:5511632/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TTCAACAGCGACGAGCACCCGGACGAGCGCTACGAGCCCCAGGAAGCCGCCGAGCACATCAACGTGATCGGCCGGGTGTTCTGGTACTCGGTGCTGGTCTGACCCCTTCCCGCCCCGCCTGACGACGGGGCCGCGTTCCGGCGGAAAAAAAATCCCGGGCAAAAAAAAAGGGGCCTGCATTGCTGCAGGCCCTTTCGGTATGGCGCACTCAGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTGCGTGTCGGTGCTTTTTCC  >  NC_008463/4659659‑4659952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑