Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 4,707,787 G→C A208A (GCG→GCC)  PA14_RS21625 → zinc‑binding alcohol dehydrogenase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084634,707,7870GC99.7% 2366.9 / NA 599A208A (GCG→GCC) PA14_RS21625zinc‑binding alcohol dehydrogenase family protein
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base C (284/312);  total (284/315)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 633 total aligned reads displayed.

GGCCAAGGCCCTGGGGGCGGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCGGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTCGCGGTGGCTCGGT  >  NC_008463/4707640‑4707933
                                                                                                                                                   |                                                                                                                                                  
ggcCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGa                                                                                                                                                  <  1:9973338/150‑1 (MQ=255)
ggcCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGa                                                                                                                                                  <  2:6011792/150‑1 (MQ=255)
ggcCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGa                                                                                                                                                  >  1:3588368/1‑150 (MQ=255)
 gcCAAGGCCCTTGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAg                                                                                                                                                 <  2:713587/150‑1 (MQ=255)
 gcCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAg                                                                                                                                                 >  2:9759944/1‑150 (MQ=255)
   cAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGt                                                                                                                                               >  1:9494148/1‑150 (MQ=255)
    aaGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTg                                                                                                                                              >  1:6908438/1‑150 (MQ=255)
      ggCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGAt                                                                                                                                            <  1:2882611/150‑1 (MQ=255)
       gCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATc                                                                                                                                           >  2:5606208/1‑150 (MQ=255)
        cccTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCt                                                                                                                                          <  1:4175154/150‑1 (MQ=255)
         ccTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCtt                                                                                                                                         <  1:6565000/150‑1 (MQ=255)
          cTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTc                                                                                                                                        <  1:7566312/150‑1 (MQ=255)
          cTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTc                                                                                                                                        >  2:2330172/1‑150 (MQ=255)
            gggggCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGa                                                                                                                                      <  1:7928371/150‑1 (MQ=255)
            gggggCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGa                                                                                                                                      >  2:3493788/1‑150 (MQ=255)
               ggCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATAc                                                                                                                                   <  2:8382889/150‑1 (MQ=255)
                 ccgAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGa                                                                                                                                 <  2:10206930/148‑1 (MQ=255)
                 ccgAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGa                                                                                                                                 <  2:2079021/148‑1 (MQ=255)
                 ccgAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGa                                                                                                                                 <  2:796451/148‑1 (MQ=255)
                   gAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGAcc                                                                                                                               >  2:3119215/1‑150 (MQ=255)
                       cGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGtt                                                                                                                           <  2:8708590/150‑1 (MQ=255)
                       cGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGtt                                                                                                                           <  2:4646447/150‑1 (MQ=255)
                       cGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGtt                                                                                                                           <  1:2794277/150‑1 (MQ=255)
                        gATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGttt                                                                                                                          <  1:10545013/150‑1 (MQ=255)
                         aTCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTc                                                                                                                         >  1:4680662/1‑150 (MQ=255)
                          tCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCt                                                                                                                        <  2:1349845/150‑1 (MQ=255)
                           cGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTg                                                                                                                       <  1:1252162/150‑1 (MQ=255)
                               gcgGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTc                                                                                                                   >  1:5800544/1‑150 (MQ=255)
                                 ggTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGa                                                                                                                 <  2:4247874/150‑1 (MQ=255)
                                  gTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAg                                                                                                                <  1:1444257/150‑1 (MQ=255)
                                    gcggcgCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAggc                                                                                                              >  2:500624/1‑150 (MQ=255)
                                    gcggcgCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAggc                                                                                                              >  2:6483452/1‑150 (MQ=255)
                                          cgAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGt                                                                                                        >  1:8894377/1‑150 (MQ=255)
                                           gaggagCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTc                                                                                                       <  2:1701538/150‑1 (MQ=255)
                                           gaggagCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTc                                                                                                       >  1:9381460/1‑150 (MQ=255)
                                              gagCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCg                                                                                                    >  2:768934/1‑150 (MQ=255)
                                               agCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCgg                                                                                                   <  2:5789703/150‑1 (MQ=255)
                                                 cAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGgcg                                                                                                 >  1:2285867/1‑150 (MQ=255)
                                                  aaCGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGgcgc                                                                                                >  1:326944/1‑150 (MQ=255)
                                                  aaCGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGgcgc                                                                                                <  1:564359/150‑1 (MQ=255)
                                                      ccAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGgc                                                                                            <  1:7078074/150‑1 (MQ=255)
                                                      ccAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGgc                                                                                            >  2:8611612/1‑150 (MQ=255)
                                                      ccAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGgc                                                                                            >  1:8528221/1‑150 (MQ=255)
                                                       cAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGgcc                                                                                           <  1:7680141/150‑1 (MQ=255)
                                                          gcgcTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGgccgcc                                                                                        <  2:5479026/150‑1 (MQ=255)
                                                            gcTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCtt                                                                                      <  1:1807223/150‑1 (MQ=255)
                                                             cTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACTGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATAGGTCCGGTGTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCGTc                                                                                     >  2:6222073/1‑150 (MQ=255)
                                                                   gcgcAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGc                                                                               <  2:2221133/150‑1 (MQ=255)
                                                                    cgcAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGACGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGcc                                                                              >  2:3953051/1‑150 (MQ=255)
                                                                      cAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTg                                                                            <  1:9800995/150‑1 (MQ=255)
                                                                      cAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTg                                                                            >  2:9150695/1‑150 (MQ=255)
                                                                       aGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTgg                                                                           <  2:52562/150‑1 (MQ=255)
                                                                       aGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTgg                                                                           <  1:2971643/150‑1 (MQ=255)
                                                                        gggCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGc                                                                          >  2:2786377/1‑150 (MQ=255)
                                                                            aTCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGatc                                                                      >  2:3784071/1‑150 (MQ=255)
                                                                             tCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGatca                                                                     <  1:67408/150‑1 (MQ=255)
                                                                                    gctgctgctGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATcgccgc                                                              >  2:9249298/1‑150 (MQ=255)
                                                                                    gctgctgctGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATcgccgc                                                              >  1:8686309/1‑150 (MQ=255)
                                                                                     ctgctgctgGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCa                                                             >  1:4466887/1‑150 (MQ=255)
                                                                                      tgctgctgGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCAc                                                            >  1:6325382/1‑150 (MQ=255)
                                                                                       gctgctgGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCAcc                                                           <  1:4093971/150‑1 (MQ=255)
                                                                                       gctgctgGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCAcc                                                           <  2:6668338/150‑1 (MQ=255)
                                                                                         tgctgGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAg                                                         >  2:2263758/1‑150 (MQ=255)
                                                                                          gctgGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGc                                                        <  2:10666662/150‑1 (MQ=255)
                                                                                             gggCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGa                                                     <  2:2914879/150‑1 (MQ=255)
                                                                                               gCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACg                                                   <  2:9534398/150‑1 (MQ=255)
                                                                                                 gAGCCGGCCGTACTGGCGGCACAGGTTGTAGGGATCTTCCCCGGCGGGGCAGAGGTGATCTTCGATACGACAGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCATTCGGTCGCCTGGCGATCATCGCCGAACCAGCCTACGGc                                                 <  2:3686810/150‑1 (MQ=255)
                                                                                                 gAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGc                                                 >  1:10418082/1‑150 (MQ=255)
                                                                                                  aGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGcc                                                <  2:3324097/150‑1 (MQ=255)
                                                                                                      ggccgAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCAtgt                                            <  2:4008539/150‑1 (MQ=255)
                                                                                                      ggccgAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCAtgt                                            >  1:10081984/1‑150 (MQ=255)
                                                                                                         cgAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTgcg                                         >  1:8859848/1‑150 (MQ=255)
                                                                                                          gAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTgcgc                                        >  2:10059162/1‑150 (MQ=255)
                                                                                                           aaCTGGCTGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCGTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTGCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGcc                                       >  2:10496522/1‑150 (MQ=255)
                                                                                                           aaCTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGcc                                       <  1:5129430/150‑1 (MQ=255)
                                                                                                            aCTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCt                                      >  1:10704810/1‑150 (MQ=255)
                                                                                                             cTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTg                                     >  1:5452844/1‑150 (MQ=255)
                                                                                                                    cacaGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGt                              <  1:10000189/150‑1 (MQ=255)
                                                                                                                      caGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTg                            <  2:9021387/150‑1 (MQ=255)
                                                                                                                       aGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTGTCTGGCTCGCGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGa                           <  1:4854130/150‑1 (MQ=255)
                                                                                                                        ggTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGaa                          <  2:4338241/150‑1 (MQ=255)
                                                                                                                          tGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAAcc                        >  1:6153574/1‑150 (MQ=255)
                                                                                                                          tGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAAcc                        >  2:7312113/1‑150 (MQ=255)
                                                                                                                            gAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTg                      <  1:7220482/150‑1 (MQ=255)
                                                                                                                            gAAGGGATCGTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTg                      <  1:9057573/150‑1 (MQ=255)
                                                                                                                              aGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTa                    <  2:7803016/150‑1 (MQ=255)
                                                                                                                              aGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTa                    <  2:8117989/150‑1 (MQ=255)
                                                                                                                               gggATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTAc                   <  1:9638621/150‑1 (MQ=255)
                                                                                                                               gggATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTAc                   <  1:2121621/150‑1 (MQ=255)
                                                                                                                                     ttCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTcgc             >  1:9257011/1‑150 (MQ=255)
                                                                                                                                      tCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTcgcg            <  2:1459822/150‑1 (MQ=255)
                                                                                                                                       ccccGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTCGCgg           <  1:3764121/150‑1 (MQ=255)
                                                                                                                                        cccGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTCGCGGt          <  1:3493788/150‑1 (MQ=255)
                                                                                                                                           ggcggggccgAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTCGCGGTGGc       <  2:1154770/150‑1 (MQ=255)
                                                                                                                                           ggcggggccgAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTCGCGGTGGc       >  2:6877860/1‑150 (MQ=255)
                                                                                                                                           ggcggggccgAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTCGCGGTGGc       >  1:8224698/1‑150 (MQ=255)
                                                                                                                                            gcggggccgAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTCGCGGTGGCt      >  2:24888/1‑150 (MQ=255)
                                                                                                                                             cggggccgAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTCGCGGTGGCTc     <  2:8852034/150‑1 (MQ=255)
                                                                                                                                              ggggccgAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCTTCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTGGAACCGGTGCCGTCGCGGGGGCTCg    >  1:2516118/1‑150 (MQ=255)
                                                                                                                                              ggggccgAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTCGCGGTGGCTCg    >  1:3170977/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGCCAAGGCCCTGGGGGCGGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCGGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTCGCGGTGGCTCGGT  >  NC_008463/4707640‑4707933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑