Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 4,947,683 A→G H166R (CAC→CGC)  PA14_RS22670 → DNA polymerase Y family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084634,947,6830AG100.0% 1245.4 / NA 324H166R (CAC→CGC) PA14_RS22670DNA polymerase Y family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (167/157);  total (167/157)

Only 100 of 324 total aligned reads displayed.

GGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAAGGTCAT‑GCGC‑ACTACACAGGACAACATTTCCATCGAGGACGAGACAGCGCTGCAACACCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTCCCGGCCGAACTGCTGT  >  NC_008463/4947536‑4947829
                                                                                                                                                     |                                                                                                                                                  
ggCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGcc                                                                                                                                                  >  1:6919683/1‑150 (MQ=255)
 gCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCa                                                                                                                                                 <  1:1786151/150‑1 (MQ=255)
 gCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCa                                                                                                                                                 <  2:3544725/150‑1 (MQ=255)
  ccACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAg                                                                                                                                                <  1:1019726/150‑1 (MQ=255)
   cACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGt                                                                                                                                               <  1:9112472/150‑1 (MQ=255)
    aCGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGtt                                                                                                                                              >  2:9287613/1‑150 (MQ=255)
    aCGATTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGtt                                                                                                                                              <  1:10479194/150‑1 (MQ=255)
     cGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTg                                                                                                                                             <  2:9763484/150‑1 (MQ=255)
     cGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTg                                                                                                                                             <  1:7252465/150‑1 (MQ=255)
        ttCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTcc                                                                                                                                          <  2:5549949/150‑1 (MQ=255)
            aGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGccc                                                                                                                                      >  1:4334192/1‑150 (MQ=255)
             ggCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCt                                                                                                                                     >  2:492420/1‑150 (MQ=255)
             ggCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCt                                                                                                                                     <  1:4211574/150‑1 (MQ=255)
             ggCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCt                                                                                                                                     >  1:4887378/1‑150 (MQ=255)
              gCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCtt                                                                                                                                    >  2:3902489/1‑150 (MQ=255)
              gCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCtt                                                                                                                                    >  2:3792813/1‑150 (MQ=255)
               cccGCTTGCGCAAGGAACTCACGGCGCTGGGCTTGCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTc                                                                                                                                   >  1:9880927/1‑150 (MQ=255)
               cccGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTc                                                                                                                                   >  2:8201710/1‑150 (MQ=255)
                ccGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTcc                                                                                                                                  >  2:5211069/1‑150 (MQ=255)
                 cGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCg                                                                                                                                 <  1:7154693/150‑1 (MQ=255)
                     tGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTgg                                                                                                                             <  2:6120022/150‑1 (MQ=255)
                      gcgcAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGa                                                                                                                            >  2:2087744/1‑150 (MQ=255)
                        gcAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGAcc                                                                                                                          >  1:8399663/1‑150 (MQ=255)
                        gcAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCAGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGAcc                                                                                                                          <  2:9414701/150‑1 (MQ=255)
                         cAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGACCCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCg                                                                                                                         >  2:1452427/1‑150 (MQ=255)
                          aaGGAACTCACGGCGCTGGGCTACCGCCCTCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGCGGGTC‑C‑GCGCGAACAGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGc                                                                                                                        <  2:6746742/150‑1 (MQ=255)
                            ggAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCt                                                                                                                      >  1:10396653/1‑150 (MQ=255)
                            ggAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCt                                                                                                                      <  1:6170159/150‑1 (MQ=255)
                             gAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTg                                                                                                                     <  1:8127471/150‑1 (MQ=255)
                               aCTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCT‑GGCG‑ACCAGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCACCGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTggg                                                                                                                   >  2:6186605/1‑150 (MQ=255)
                                 tCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGAc                                                                                                                 <  2:4253761/150‑1 (MQ=255)
                                  cACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGAct                                                                                                                >  2:10016774/1‑150 (MQ=255)
                                   aCGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGActc                                                                                                               >  2:4774885/1‑150 (MQ=255)
                                    cGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGActct                                                                                                              <  2:2116528/150‑1 (MQ=255)
                                    cGGCGCTGGGCGTCCGCCATCGCCTGTGCCTGGCGCCGACCCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACGGCCTGGGActct                                                                                                              <  1:3968074/150‑1 (MQ=255)
                                      gcgcTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTcg                                                                                                            >  2:813926/1‑150 (MQ=255)
                                        gcTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTcgcg                                                                                                          <  1:9433007/150‑1 (MQ=255)
                                        gcTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTcgcg                                                                                                          <  2:9635398/150‑1 (MQ=255)
                                          tGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCggg                                                                                                        <  1:3578126/150‑1 (MQ=255)
                                           gggCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGa                                                                                                       <  2:10688595/150‑1 (MQ=255)
                                                tCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTgg                                                                                                  >  1:8998305/1‑150 (MQ=255)
                                                 ccGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGc                                                                                                 <  2:1306929/150‑1 (MQ=255)
                                                    ccATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGt                                                                                              >  1:2387151/1‑150 (MQ=255)
                                                    ccATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGt                                                                                              >  2:250495/1‑150 (MQ=255)
                                                    ccATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGt                                                                                              <  1:443434/150‑1 (MQ=255)
                                                    ccATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGt                                                                                              <  1:6416618/150‑1 (MQ=255)
                                                     cATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTc                                                                                             >  2:1617719/1‑150 (MQ=255)
                                                     cATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTc                                                                                             >  2:1988390/1‑150 (MQ=255)
                                                      aTCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCg                                                                                            >  1:5781852/1‑150 (MQ=255)
                                                      aTCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCg                                                                                            <  1:5265626/150‑1 (MQ=255)
                                                       tCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGc                                                                                           <  1:7974375/150‑1 (MQ=255)
                                                        cgcctgcgcctgGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGcc                                                                                          <  2:2249568/150‑1 (MQ=255)
                                                        cGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGcc                                                                                          <  2:2252460/150‑1 (MQ=255)
                                                          ccTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCt                                                                                        <  2:9091733/150‑1 (MQ=255)
                                                          ccTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCt                                                                                        >  1:10648071/1‑150 (MQ=255)
                                                           cTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTc                                                                                       >  2:5321359/1‑150 (MQ=255)
                                                            tgtgCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCCGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCg                                                                                      >  2:6547064/1‑150 (MQ=255)
                                                            tgtgCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCg                                                                                      >  2:7452380/1‑150 (MQ=255)
                                                             gtgCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCgg                                                                                     <  2:7676647/150‑1 (MQ=255)
                                                              tgCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGa                                                                                    <  2:2315042/150‑1 (MQ=255)
                                                              tgCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGa                                                                                    <  2:3065043/150‑1 (MQ=255)
                                                               gCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGAc                                                                                   >  2:10512087/1‑150 (MQ=255)
                                                                   ggCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCACCGCCAGTTGTCCGCCCTTCCGCTGGACCGCCGGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCAt                                                                               >  2:888467/1‑150 (MQ=255)
                                                                   ggCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCAt                                                                               >  1:3209356/1‑150 (MQ=255)
                                                                     cgcCGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATgg                                                                             <  2:8579698/150‑1 (MQ=255)
                                                                        cGAACCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGAc                                                                          >  2:4640919/1‑150 (MQ=255)
                                                                           aCCCGGTGGCGGCGAGGGTCCTGGCGA‑AC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTgc                                                                       >  2:3508821/1‑150 (MQ=255)
                                                                                                                   ggcctgtCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGATGCCGCGCgccgcc                                 >  2:310118/7‑150 (MQ=255)
                                                                                                                    gcctggccATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGccc                                <  1:9064096/144‑1 (MQ=255)
                                                                                                                     cctggccATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCTTCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCt                               <  1:2975391/145‑1 (MQ=255)
                                                                                                                     cctggccATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCt                               >  2:2196566/6‑150 (MQ=255)
                                                                                                                        ggccATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGc                            >  2:6896830/3‑150 (MQ=255)
                                                                                                                         gccATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGcc                           <  1:1459802/149‑1 (MQ=255)
                                                                                                                          ccATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCCTGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGccc                          >  2:6709687/1‑150 (MQ=255)
                                                                                                                          ccATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGccc                          <  2:5892219/150‑1 (MQ=255)
                                                                                                                          ccATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGccc                          <  2:10286363/150‑1 (MQ=255)
                                                                                                                            aacGAGGACGAGACAGCGCTGCCACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCACGGACGCATGGGACCGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGt                        <  1:3856219/148‑1 (MQ=255)
                                                                                                                            aTCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGt                        <  1:7739199/150‑1 (MQ=255)
                                                                                                                             tCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTc                       <  2:1880211/150‑1 (MQ=255)
                                                                                                                               gAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGc                     >  2:3244358/1‑150 (MQ=255)
                                                                                                                               gAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGc                     >  1:1978488/1‑150 (MQ=255)
                                                                                                                                aGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCt                    <  2:4343996/150‑1 (MQ=255)
                                                                                                                                 ggACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCtt                   >  2:10150955/1‑150 (MQ=255)
                                                                                                                                  gACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTc                  <  1:6507888/150‑1 (MQ=255)
                                                                                                                                    cGAGCCAGCGCTGCAACGCCAGTTGTCCGCCCGTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGCCGCATGGGCCTGCGCCATCTGCGCCCGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTccc                <  2:6986905/150‑1 (MQ=255)
                                                                                                                                    cGAGACATCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTccc                <  2:6056090/150‑1 (MQ=255)
                                                                                                                                     gagaCAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTCCCg               >  2:7297340/1‑150 (MQ=255)
                                                                                                                                     gagaCAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTCCCg               <  2:8333809/150‑1 (MQ=255)
                                                                                                                                      agaCAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTCCCgg              >  1:7429952/1‑150 (MQ=255)
                                                                                                                                       gaCAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGCCTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTCCCGGc             >  2:9457755/1‑150 (MQ=255)
                                                                                                                                        aCAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTCCCGGcc            <  2:7104950/150‑1 (MQ=255)
                                                                                                                                         cAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTCCCGGCCg           >  1:2334400/1‑150 (MQ=255)
                                                                                                                                          aGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTCCCGGCCGa          >  2:1192145/1‑150 (MQ=255)
                                                                                                                                          aGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTCCCGGCCGa          <  1:6543415/150‑1 (MQ=255)
                                                                                                                                            cgcTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTCCCGGCCGAAc        <  2:3614985/150‑1 (MQ=255)
                                                                                                                                               tGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTCCCGGCCGAActgc     <  2:2838390/150‑1 (MQ=255)
                                                                                                                                                gCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTCCCGGCCGAActgct    >  1:8294277/1‑150 (MQ=255)
                                                                                                                                                 cAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTCCCGGCCGAActgctg   >  1:5546085/1‑150 (MQ=255)
                                                                                                                                                  aaCGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTCCCGGCCGAACTGCTGt  <  2:239160/150‑1 (MQ=255)
                                                                                                                                                  aaCGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTCCCGGCCGAACTGCTGt  <  2:2677540/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
GGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAAGGTCAT‑GCGC‑ACTACACAGGACAACATTTCCATCGAGGACGAGACAGCGCTGCAACACCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTCCCGGCCGAACTGCTGT  >  NC_008463/4947536‑4947829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑