Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 41,756 G→A R208R (CGG→CGA)  tpsB2 → two‑partner secretion system transporter TpsB2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00846341,7560GA100.0% 1526.5 / NA 605R208R (CGG→CGA) tpsB2two‑partner secretion system transporter TpsB2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (320/285);  total (320/285)

Only 100 of 605 total aligned reads displayed.

GCGCGAACTGGCGATGAGCTTTCCGGGCAGGACCGGCGAGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGTCGGGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGACAGCCCGCTGGGCCTGGCCGACCAGCTCAACCT  >  NC_008463/41609‑41899
                                                                                                                                                   |                                                                                                                                               
gcgcgAACTGGCGATGAGCTTTCCGGGCAGGACCGGCGAGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGt                                                                                                                                               >  2:6456307/1‑150 (MQ=21)
    gAACTGGCGATGAGCTTTCCGGGCAGGACCGGCGAGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGt                                                                                                                                           >  1:10527528/1‑150 (MQ=21)
      aCTGGCGATGAGCTTTCCGGGCAGGACCGGCGAGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGtct                                                                                                                                         <  1:9560763/150‑1 (MQ=21)
       cTGGCGATGAGCTTTCCGGGCAGGACCGGCGAGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGtctc                                                                                                                                        >  2:4306784/1‑150 (MQ=21)
           cGATGAGCTTTCCGGGCAGGACCGGCGAGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAgg                                                                                                                                    >  2:5708286/1‑150 (MQ=21)
            gATGAGCTTTCCGGGCAGGACCGGCGAGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAggg                                                                                                                                   <  1:8803180/150‑1 (MQ=21)
             aTGAGCTTTCCGGGCAGGACCGGCGAGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGc                                                                                                                                  <  1:6231172/150‑1 (MQ=21)
             aTGAGCTTTCCGGGCAGGACCGGCGAGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGc                                                                                                                                  <  2:7133472/150‑1 (MQ=21)
             aTGAGCTTTCCGGGCAGGACCGGCGAGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGc                                                                                                                                  >  1:3856543/1‑150 (MQ=21)
                aGCTTTCCGGGCAGGACCGGCGAGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAg                                                                                                                               <  2:8900870/150‑1 (MQ=21)
                 gCTTTCCGGGCAGGACCGGCGAGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAgc                                                                                                                              >  1:6662887/1‑150 (MQ=21)
                 gCTTTCCGGGCAGGACCGGCGAGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAgc                                                                                                                              >  1:4653669/1‑150 (MQ=21)
                  cTTTCCGGGCAGGACCGGCGAGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAgcg                                                                                                                             <  2:7761945/150‑1 (MQ=21)
                   tttCCGGGCAGGACCGGCGAGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAgcgc                                                                                                                            <  1:8328540/150‑1 (MQ=21)
                     tCCGGGCAGGACCGGCGAGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGa                                                                                                                          >  2:5561765/1‑150 (MQ=21)
                     tCCGGGCAGGACCGGCGAGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGa                                                                                                                          <  2:189289/150‑1 (MQ=21)
                       cGGGCAGGACCGGCGAGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACa                                                                                                                        >  1:4967820/1‑150 (MQ=21)
                         ggCAGGACCGGCGAGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAg                                                                                                                      >  1:7385302/1‑150 (MQ=21)
                          gCAGGACCGGCGAGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGc                                                                                                                     >  2:4539284/1‑150 (MQ=21)
                            aGGACCGGCGAGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGccc                                                                                                                   <  2:8673919/150‑1 (MQ=21)
                            aGGACCGGCGAGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGccc                                                                                                                   <  1:3980695/150‑1 (MQ=21)
                             ggACCGGCGAGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCt                                                                                                                  >  1:7127425/1‑150 (MQ=21)
                             ggACCGGCGAGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCt                                                                                                                  >  1:10616893/1‑150 (MQ=21)
                             ggACCGGCGAGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCt                                                                                                                  >  2:1574356/1‑150 (MQ=21)
                                ccGGCGAGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGc                                                                                                               >  1:9763802/1‑150 (MQ=21)
                                   gCGAGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCggg                                                                                                            >  2:5290629/1‑150 (MQ=21)
                                      aGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGtct                                                                                                         >  2:5367129/1‑150 (MQ=21)
                                       gctgctCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGtctc                                                                                                        <  2:1005798/150‑1 (MQ=18)
                                       gctgctCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGtctc                                                                                                        >  1:3423861/1‑150 (MQ=18)
                                          gctCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGc                                                                                                     <  1:3677125/150‑1 (MQ=17)
                                          gctCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGc                                                                                                     >  1:3257880/1‑150 (MQ=17)
                                           ctCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGcc                                                                                                    >  1:6305906/1‑150 (MQ=17)
                                             cGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCAc                                                                                                  <  1:4872798/150‑1 (MQ=17)
                                             cGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCAc                                                                                                  <  2:10671181/150‑1 (MQ=17)
                                                ccTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCAcgcg                                                                                               >  2:9403311/1‑150 (MQ=17)
                                                ccTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCAcgcg                                                                                               >  1:10395945/1‑150 (MQ=17)
                                                 cTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACgcgc                                                                                              <  2:3965884/150‑1 (MQ=17)
                                                  tGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGca                                                                                             >  2:3452568/1‑150 (MQ=17)
                                                  tGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGca                                                                                             <  2:10125727/150‑1 (MQ=17)
                                                   gcgcGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGcaa                                                                                            >  1:10023093/1‑150 (MQ=17)
                                                    cgcgAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGcaac                                                                                           <  2:1741044/150‑1 (MQ=17)
                                                     gcgAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGcaaca                                                                                          <  1:10571528/150‑1 (MQ=17)
                                                       gAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCaacaac                                                                                        <  1:5600405/150‑1 (MQ=17)
                                                         aCTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAacga                                                                                      >  2:8539574/1‑150 (MQ=17)
                                                         aCTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAacga                                                                                      <  2:7488081/150‑1 (MQ=17)
                                                         aCTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAacga                                                                                      <  2:1333233/150‑1 (MQ=17)
                                                          cTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAacgac                                                                                     >  1:5837983/1‑150 (MQ=17)
                                                           tGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAacgacg                                                                                    >  1:2349893/1‑150 (MQ=17)
                                                           tGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAacgacg                                                                                    <  1:2836165/150‑1 (MQ=17)
                                                            ggAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACgg                                                                                   >  2:3333249/1‑150 (MQ=17)
                                                              aGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCg                                                                                 <  1:6152622/150‑1 (MQ=17)
                                                              aGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCg                                                                                 >  1:1397294/1‑150 (MQ=17)
                                                              aGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCg                                                                                 >  1:9311546/1‑150 (MQ=17)
                                                               gCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGa                                                                                <  1:7525500/150‑1 (MQ=17)
                                                                  aCTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGt                                                                             >  1:418114/1‑150 (MQ=17)
                                                                    tGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCACCTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCa                                                                           <  2:8259827/150‑1 (MQ=12)
                                                                    tGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCa                                                                           <  1:1719336/150‑1 (MQ=17)
                                                                     ggTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAg                                                                          <  2:266408/150‑1 (MQ=17)
                                                                       tCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCa                                                                        >  2:90332/1‑150 (MQ=17)
                                                                         gACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCAcc                                                                      <  2:9710592/150‑1 (MQ=17)
                                                                          aCCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCg                                                                     >  2:4909644/1‑150 (MQ=17)
                                                                             aGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCg                                                                  >  2:118583/1‑150 (MQ=17)
                                                                               ttGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGag                                                                <  2:8106768/150‑1 (MQ=17)
                                                                               ttGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGag                                                                >  2:1020135/1‑150 (MQ=17)
                                                                                     cGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGcagcag                                                          <  2:2187240/150‑1 (MQ=17)
                                                                                       ccTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGAt                                                        >  1:2007726/1‑150 (MQ=17)
                                                                                        cTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATg                                                       >  1:1253778/1‑150 (MQ=17)
                                                                                          gCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATggg                                                     >  1:6442101/1‑150 (MQ=17)
                                                                                                gcgcCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTggg                                               >  1:4356787/1‑150 (MQ=17)
                                                                                                  gcCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTc                                             <  1:907169/150‑1 (MQ=18)
                                                                                                   ccAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCt                                            >  1:5351289/1‑150 (MQ=21)
                                                                                                   ccAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCt                                            >  1:2270369/1‑150 (MQ=21)
                                                                                                    cAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTg                                           <  2:2347873/150‑1 (MQ=21)
                                                                                                      ggCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGa                                         <  1:6865873/150‑1 (MQ=21)
                                                                                                         gcAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTg                                      >  1:9248539/1‑150 (MQ=21)
                                                                                                           aaCTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTggg                                    >  2:371305/1‑150 (MQ=21)
                                                                                                            aCTGGAGCTAGGGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGa                                   <  2:2888352/150‑1 (MQ=255)
                                                                                                             cTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGAc                                  >  1:8093977/1‑150 (MQ=21)
                                                                                                                 aGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGACAGcc                              <  1:8851986/150‑1 (MQ=21)
                                                                                                                  gCTAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGACAGccc                             >  2:2959449/1‑150 (MQ=21)
                                                                                                                    tAGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGACAGCCCGc                           <  2:7942265/150‑1 (MQ=18)
                                                                                                                     aGTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGACAGCCCGCt                          >  1:6559515/1‑150 (MQ=17)
                                                                                                                      gTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGACAGCCCGCTg                         <  2:8320265/150‑1 (MQ=17)
                                                                                                                      gTGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGACAGCCCGCTg                         >  1:81682/1‑150 (MQ=17)
                                                                                                                       tGCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGACAGCCCGCTgg                        >  2:63128/1‑150 (MQ=17)
                                                                                                                        gCCCGGCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGACAGCCCGCTggg                       <  2:9216838/150‑1 (MQ=17)
                                                                                                                             gCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGACAGCCCGCTGGGCCTgg                  <  2:1040601/150‑1 (MQ=17)
                                                                                                                             gCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGACAGCCCGCTGGGCCTgg                  <  1:10773881/150‑1 (MQ=17)
                                                                                                                             gCAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGACAGCCCGCTGGGCCTgg                  >  1:3061548/1‑150 (MQ=17)
                                                                                                                              cAGCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGACAGCCCGCTGGGCCTGGc                 <  1:9916881/150‑1 (MQ=17)
                                                                                                                                gCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGACAGCCCGCTGGGCCTGGCCg               >  2:782022/1‑150 (MQ=17)
                                                                                                                                gCGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGACAGCCCGCTGGGCCTGGCCg               <  2:7306936/150‑1 (MQ=17)
                                                                                                                                 cGAGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGACAGCCCGCTGGGCCTGGCCGa              <  2:510418/150‑1 (MQ=17)
                                                                                                                                   aGGTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGACAGCCCGCTGGGCCTGGCCGAcc            >  2:7020457/1‑150 (MQ=17)
                                                                                                                                     gTCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGACAGCCCGCTGGGCCTGGCCGACCAg          >  1:6042695/1‑150 (MQ=17)
                                                                                                                                      tCGGCGGCAGCCGAGTGCGTCTCAAGGGCGAGCGCGACACGCCCTGGCGGGTCTCCGCCACGCGCAACCCCGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGACAGCCCGCTGGGCCTGGCCGACCAGc         <  1:4453164/150‑1 (MQ=11)
                                                                                                                                         gcggcAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGACAGCCCGCTGGGCCTGGCCGACCAGCTCa      >  2:6641037/1‑150 (MQ=17)
                                                                                                                                          cggcAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGACAGCCCGCTGGGCCTGGCCGACCAGCTCaa     >  2:5838150/1‑150 (MQ=17)
                                                                                                                                          cggcAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGACAGCCCGCTGGGCCTGGCCGACCAGCTCaa     <  1:190672/150‑1 (MQ=17)
                                                                                                                                           ggcAGCCGAGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGACAGCCCGCTGGGCCTGGCCGACCAGCTCAAc    <  1:7708395/150‑1 (MQ=17)
                                                                                                                                                   |                                                                                                                                               
GCGCGAACTGGCGATGAGCTTTCCGGGCAGGACCGGCGAGCTGCTCGACCTGCGCGAACTGGAGCAACTGGTCGACCAGTTGAGTCGCCTGCCGTCGCGCCAGGCGCAACTGGAGCTAGTGCCCGGCAGCGAGGTCGGCGGCAGTCGGGTGCGTCTCAAGGGCGAGCGCGACAAGCCCTGGCGGGTCTCCGCCACGCGCAACAACGACGGCGACGTCAGCACCGGCGAGCAGCAGATGGGCCTGGGTCTGGACTGGGACAGCCCGCTGGGCCTGGCCGACCAGCTCAACCT  >  NC_008463/41609‑41899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑