BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	5,809,315	G→C	intergenic (‑201/+18)	rpsF ← / ← rlmB	30S ribosomal protein S6/23S rRNA (guanosine(2251)‑2'‑O)‑methyltransferase RlmB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	5,809,315	0	G	C	100.0%	1384.7 / NA	366	intergenic (‑201/+18)	rpsF/rlmB	30S ribosomal protein S6/23S rRNA (guanosine(2251)‑2'‑O)‑methyltransferase RlmB
Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (220/146); total (220/146)

Only 100 of 366 total aligned reads displayed.

TTGGCTTGCCTGGTGGAGAGGCGAACGAACGCCTGCCCGCGCGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATGGGCGCGCGGGGCCCCCCCCGGGGGGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTTGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAG  >  NC_008463/5809175‑5809461

ttcttggcttgcctggtggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCg                                                                                                                                          >  1:2093110‑M2/125‑150 (MQ=255)

 tcttggcttgcctggtggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGc                                                                                                                                         >  2:2711909‑M2/124‑150 (MQ=255)

  cttggcttgcctggtggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCa                                                                                                                                        >  1:1745496‑M2/123‑150 (MQ=255)

   ttggcttgcctggtggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCAc                                                                                                                                       <  1:3016224‑M2/29‑1 (MQ=255)

    tggcttgcctggtggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctcgagaagggcaaccggcgaagcaaggaagattggcgaatatttgtccagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCAcg                                                                                                                                      >  2:4508711‑M2/121‑150 (MQ=255)

       cttgcctggtggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACgcgc                                                                                                                                   >  2:1472553‑M2/118‑150 (MQ=255)

          gcctggtggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCa                                                                                                                                >  2:8797672‑M2/115‑150 (MQ=255)

           cctggtggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCAc                                                                                                                               >  2:7972215‑M2/114‑150 (MQ=255)

            ctggtggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACt                                                                                                                              >  1:8196084‑M2/113‑150 (MQ=255)

             tggtggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggacgattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTc                                                                                                                             >  2:5163271‑M2/112‑150 (MQ=255)

              ggtggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTcc                                                                                                                            >  1:407676‑M2/111‑150 (MQ=255)

                 ggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGc                                                                                                                         >  2:6456972‑M2/108‑150 (MQ=255)

                  gagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattatagagaagggcaaccggcgaagcatggacgattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCg                                                                                                                        <  1:2576999‑M2/44‑1 (MQ=255)

                   agaggcgaacgaacgcctgcccgcgcggcacggggcgacattctagagacgggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCgg                                                                                                                       <  2:7231448‑M2/45‑1 (MQ=255)

                     aggcgaacgaacgcctgcccgcgcggcaaggggcgaccttctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCgggg                                                                                                                     >  2:7632971‑M2/104‑150 (MQ=255)

                     aggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCgggg                                                                                                                     <  1:851871‑M2/47‑1 (MQ=255)

                      ggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGt                                                                                                                    <  2:9689553‑M2/48‑1 (MQ=255)

                      ggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGt                                                                                                                    >  1:4845481‑M2/103‑150 (MQ=255)

                          aacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTgcgc                                                                                                                <  2:5968897‑M2/52‑1 (MQ=255)

                           acgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCt                                                                                                               >  1:739463‑M2/98‑150 (MQ=255)

                           acgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCt                                                                                                               >  1:5038306‑M2/98‑150 (MQ=255)

                              aacgcctgcccgcgcggcaaggggcgacattctagagaagggcacccggcgcagcacggaagattggcgaatctttgtccagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGc                                                                                                            <  1:9032241‑M2/56‑1 (MQ=255)

                              aacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggagaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCACGCACGCGCTCACTCCAGCGGGGTGCGCTGGc                                                                                                            >  2:3942736‑M2/95‑150 (MQ=255)

                                cgcctgcccgcgcggccaggggcgacattctagagtagggcaaccggcgtagcaaggaagattggcgaagatgtgtacagcggagggacgggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCgg                                                                                                          >  2:5449429‑M2/93‑150 (MQ=255)

                                 gcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaataattgtccagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCgga                                                                                                         <  2:4085247‑M2/59‑1 (MQ=255)

                                  cctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtccagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCggag                                                                                                        >  2:1219535‑M2/91‑150 (MQ=255)

                                    tgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggagaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACACAAGCGGGGTGCGCTGGCggagga                                                                                                      >  1:8636413‑M2/89‑150 (MQ=255)

                                     gcccgcgcggcaaggggcgccattctcgagacgggcaaccggcgaagcaaggaagattggcgaatattggtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGAt                                                                                                     <  2:6591601‑M2/63‑1 (MQ=255)

                                     gcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGAt                                                                                                     <  2:6355984‑M2/63‑1 (MQ=255)

                                     gcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCACCCTCCAGCGGGGTGCGCTGgcggaggtt                                                                                                     >  2:4881083‑M2/88‑148 (MQ=255)

                                        cgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATctc                                                                                                  <  2:870378‑M2/66‑2 (MQ=255)

                                         gcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGAtctcg                                                                                                 >  1:6541606‑M2/84‑148 (MQ=255)

                                             ggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGACCa                                                                                             <  1:2633682‑M2/71‑1 (MQ=255)

                                              gcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAg                                                                                            >  1:5719839‑M2/79‑150 (MQ=255)

                                              gcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAg                                                                                            >  2:3448548‑M2/79‑150 (MQ=255)

                                                aaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGc                                                                                          >  2:9441956‑M2/77‑150 (MQ=255)

                                                 aggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCa                                                                                         >  2:1731961‑M2/76‑150 (MQ=255)

                                                 aggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCa                                                                                         >  1:9056327‑M2/76‑150 (MQ=255)

                                                    ggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGAc                                                                                      >  2:7305629‑M2/73‑150 (MQ=255)

                                                      cgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGAGCTGGCGGAGGATCTCGAACAGGCAGACGc                                                                                    >  2:8442642‑M2/71‑150 (MQ=255)

                                                        acattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCg                                                                                  >  2:9004866‑M2/69‑150 (MQ=255)

                                                        acattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCg                                                                                  >  1:10162087‑M2/69‑150 (MQ=255)

                                                          attctagagaagggcaaccggcgaagcaaggaagatgggcgaatatttgtccagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGt                                                                                <  1:3943056‑M2/84‑1 (MQ=255)

                                                            tctcgataagggcaaccggcgaagcaaggaagatgggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTgg                                                                              <  1:1851030‑M2/86‑1 (MQ=255)

                                                            tctagagaagggctaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTgg                                                                              <  2:4386248‑M2/86‑1 (MQ=255)

                                                            tctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTgg                                                                              <  1:1383769‑M2/86‑1 (MQ=255)

                                                             ctagagacgggccaccggcgaagcaaggaagatgggcgaagatttggacagcggatggaaaggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGc                                                                             <  1:7823884‑M2/87‑1 (MQ=255)

                                                             ctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGc                                                                             >  2:1512495‑M2/64‑150 (MQ=255)

                                                              tagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCg                                                                            >  2:4281062‑M2/63‑150 (MQ=255)

                                                                gagaagggcaaccggcgaagcaaggaagattggcgaatatttgttcagcggatggaagggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCTCTCCAGCGGGGTGCGCTGGCGGAGGTTATCGATCAGGCCGACGCCGGTGGCGAc                                                                          >  1:4646799‑M2/61‑150 (MQ=255)

                                                                gagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGAc                                                                          >  2:1836162‑M2/61‑150 (MQ=255)

                                                                     gggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCgaga                                                                     <  1:9768057‑M2/95‑1 (MQ=255)

                                                                       gcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACg                                                                   >  2:5030913‑M2/54‑150 (MQ=255)

                                                                       gcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACg                                                                   <  2:2921515‑M2/97‑1 (MQ=255)

                                                                       gcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACg                                                                   >  2:10425155‑M2/54‑150 (MQ=255)

                                                                        caaccggcgaagcaaggaagattggcgaatattggtaccgcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGt                                                                  <  1:7075077‑M2/98‑1 (MQ=255)

                                                                         aaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGtt                                                                 >  2:9528562‑M2/52‑150 (MQ=255)

                                                                          accggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTc                                                                >  2:4626343‑M2/51‑150 (MQ=255)

                                                                          accggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGGGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTc                                                                >  2:4659764‑M2/51‑150 (MQ=255)

                                                                             ggcgaagcaaggaagattggctaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAgg                                                             <  1:125929‑M2/103‑1 (MQ=255)

                                                                              gcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGgc                                                            <  2:7388299‑M2/104‑1 (MQ=255)

                                                                                gaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGgctg                                                          >  1:9442793‑M2/45‑150 (MQ=255)

                                                                                  agcaaggaagattggcgaatatttgtaccgcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGgctgct                                                        <  1:1003071‑M2/108‑1 (MQ=255)

                                                                                     aaggaagattggcgaatatttgtacagcggatggaatggCTCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTAACTCCAGCGGGGTGCGCTGGCGGAGGATCACGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCATGCTGCTgaa                                                     >  1:2062258‑M2/40‑149 (MQ=255)

                                                                                       ggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCCCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGc                                                   >  1:7196814‑M2/38‑150 (MQ=255)

                                                                                         aagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACGCCAGCGGGGGGCGCTGGCGGCGGATCTCGAACAGGCAGACGCCGGGGGCGACCGAGCAGTTCAGGCTGCGGACGCTg                                                 >  2:7425894‑M2/36‑150 (MQ=255)

                                                                                           gattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGcc                                               >  1:10595252‑M2/34‑150 (MQ=255)

                                                                                             ttggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCgg                                             >  2:8956817‑M2/32‑150 (MQ=255)

                                                                                             ttggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCAATCCAGCGGGGTGCGCTGGCGGAGGATATCGAACAGGCCGACGCCGGGGGCGACCGAGACGTTCAGGCTGCGGTCGCAGCCgg                                             >  1:7300508‑M2/32‑150 (MQ=255)

                                                                                              tggcgtatatttgtacagcggttggtatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGc                                            <  1:3096401‑M2/120‑1 (MQ=255)

                                                                                               ggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGcc                                           >  2:6811412‑M2/30‑150 (MQ=255)

                                                                                                gcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCa                                          >  2:8244704‑M2/29‑150 (MQ=255)

                                                                                                 cgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTTCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCAt                                         >  2:227183‑M2/28‑150 (MQ=255)

                                                                                                   aatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCg                                       <  1:3745991‑M2/125‑1 (MQ=255)

                                                                                                      atttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCa                                    >  2:3651421‑M2/23‑150 (MQ=255)

                                                                                                            accgcggttggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTAAGGCTGCTGACGCTGCCGGCCATCGTCAACCTgg                              <  2:2026893‑M2/134‑1 (MQ=255)

                                                                                                             cagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGc                             <  2:5318349‑M2/135‑1 (MQ=255)

                                                                                                                cggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGCAACTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAg                          <  2:322200‑M2/138‑1 (MQ=255)

                                                                                                                 ggatggaatggCGCGGGGCCGCGCCC‑CGGCGGCCCCGCACGCGCTCACACCAGCGGGGTGCGCTGGCGGAGGCTCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGAAGCTGCCGGCCATCGGCAACCAGGCGAgg                         >  1:7674019‑M2/12‑150 (MQ=255)

                                                                                                                 ggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAgg                         <  2:4466827‑M2/139‑1 (MQ=255)

                                                                                                                  gatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGGCGGTGGCGACCGAGTCGTTCAGGCTGCGGACGCTGGCGGCCATCGGCAACCTGGCGAGGa                        >  1:6797167‑M2/11‑150 (MQ=255)

                                                                                                                  gatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGa                        >  2:9150114‑M2/11‑150 (MQ=255)

                                                                                                                  gatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACGCCAGCGGGGTGCGCTGGCGGAGGCTCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGGTAAGGCTGCTGTCGCTGCCGGCCCTCGGCAACCTGGCGaggg                        >  1:6092347‑M2/11‑149 (MQ=255)

                                                                                                                    tggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAg                      <  2:1997269‑M2/142‑1 (MQ=255)

                                                                                                                    tggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAg                      <  2:7016080‑M2/142‑1 (MQ=255)

                                                                                                                    tggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACACCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACCCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCAGCCGGCCATAGGCAACCAGGCGAGGAAg                      >  1:8463258‑M2/9‑150 (MQ=255)

                                                                                                                    tggaatggCGCGGGG‑CCGCCCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCAGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTTACGCTGCCGGCCCTCGGCAACCAGGCGAGGAAg                      >  1:10710616‑M2/9‑150 (MQ=255)

                                                                                                                      gaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCCCACCCGCTCACACCAGCGGGGTGCGCTGGCGGATGATCTCGAACAGGCAGACGCCGGTTGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCAACGGCAAACTGGCGAGGTAGTc                    >  1:431092‑M2/7‑150 (MQ=255)

                                                                                                                       aatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCCTCGGCAACCTGGCGAGGAAGTCg                   >  1:5448492‑M2/6‑150 (MQ=255)

                                                                                                                       aatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCg                   >  2:2175044‑M2/6‑150 (MQ=255)

                                                                                                                          ggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAg                >  1:5554354‑M2/3‑150 (MQ=255)

                                                                                                                           gCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGt               <  2:3096002‑M2/149‑1 (MQ=255)

                                                                                                                           gCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGt               >  1:10441525‑M2/2‑150 (MQ=255)

                                                                                                                                  ggccgcgccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGAGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCGGCTGACGCTGCCGGCCATCGCCAACCTGGCGAGGAAGGCGCAGTGCTcgc         <  2:3752061/143‑1 (MQ=255)

                                                                                                                                  ggccgcgccGCGGCGGCCCCGCACGCGCTCACTCCACCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCCGACGCCGGTGGCGACCGAGACGTTCAGGCTGCGGACGCGGCGGGCCATCGGCAACCTGGCGTGGAAGTCGCTGTGCTcgc         >  1:3249006/8‑150 (MQ=255)

                                                                                                                                   gccgcgccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTcgcg        >  2:1317938/7‑150 (MQ=255)

                                                                                                                                    ccgcgccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCgg       >  2:10224421/6‑150 (MQ=255)

                                                                                                                                     cgcgccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCggg      >  2:10576286/5‑150 (MQ=255)

                                                                                                                                       cgccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTc    <  1:2301750/148‑1 (MQ=255)

                                                                                                                                         ccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAg  <  1:3892281/150‑1 (MQ=255)

TTGGCTTGCCTGGTGGAGAGGCGAACGAACGCCTGCCCGCGCGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATGGGCGCGCGGGGCCCCCCCCGGGGGGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTTGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAG  >  NC_008463/5809175‑5809461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`