Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 5,905,079 A→G F731F (TTT→TTC)  glnE ← bifunctional [glutamate‑‑ammonia ligase]‑adenylyl‑L‑tyrosine phosphorylase/[glutamate‑‑ammonia‑ligase] adenylyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084635,905,0790AG100.0% 1755.2 / NA 448F731F (TTT→TTC) glnEbifunctional [glutamate‑‑ammonia ligase]‑adenylyl‑L‑tyrosine phosphorylase/[glutamate‑‑ammonia‑ligase] adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (264/184);  total (264/184)

Only 100 of 451 total aligned reads displayed.

CAGGCGCATGTCGACCTCGTAGAGGGTGCCGGATGGCGTCTGCGCGGTGAGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATAAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGGCCGGCCGTGACGCTGCACCAACTGCCGCCAGGCCAGT  >  NC_008463/5904932‑5905223
                                                                                                                                                   |                                                                                                                                                
cAGGCGCATGTCGACCTCGTAGAGGGTGCCGGATGGCGTCTGCGCGGTGAGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATgaa                                                                                                                                                <  1:3073078/150‑1 (MQ=255)
     gcATGTCGACCTCGTAGAGGGTGCCGGATGGCGTCTGCGCGGTGAGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCa                                                                                                                                           <  2:1842860/150‑1 (MQ=255)
       ctGTCGACCTCGTAGAGGGTGCCGGATGGCGTCTGCGCGGTGAGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAgg                                                                                                                                         <  1:9289457/149‑1 (MQ=255)
             aCCTCGTAGAGGGTGCCGGATGGCGTCTGCGCGGTGAGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAgg                                                                                                                                   >  2:7807974/1‑150 (MQ=255)
                 cGTAGAGGGTGCCGGATGGCGTCTGCGCGGTGAGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCg                                                                                                                               <  2:6939997/150‑1 (MQ=255)
                 cGTAGAGGGTGCCGGATGGCGTCTGCGCGGTGAGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCg                                                                                                                               <  1:2496132/150‑1 (MQ=255)
                  gTAGAGGGTGCCGGATGGCGTCTGCGCGGTGAGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACCCCAGGTCGAGGTCCGa                                                                                                                              <  2:8363869/150‑1 (MQ=255)
                     gagGGTGCCGGATGGCGTCTGCGCGGTGAGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGcc                                                                                                                           >  1:5909167/1‑150 (MQ=255)
                     gagGGTGCCGGATGGCGTCTGCGCGGTGAGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGcc                                                                                                                           >  1:540348/1‑150 (MQ=255)
                      agGGTGCCGGATGGCGTCTGCGCGGTGAGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCa                                                                                                                          >  2:1999818/1‑150 (MQ=255)
                        ggTGCCGGATGGCGTCTGCGCGGTGAGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATg                                                                                                                        <  2:3096360/150‑1 (MQ=255)
                          tGCCGGATGGCGTCTGCGCGGTGAGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGAc                                                                                                                      >  1:1000992/1‑150 (MQ=255)
                           gCCGGATGGCGTCTGCGCGGTGAGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCACGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGAcc                                                                                                                     <  2:4077863/150‑1 (MQ=255)
                               gATGGCGTCTGCGCGGTGAGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAAc                                                                                                                 <  2:9492137/150‑1 (MQ=255)
                                ttGGCGTCTGCGCGGTGAGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACt                                                                                                                >  1:4658575/2‑150 (MQ=255)
                                aTGGCGTCTGCGCGGTGAGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACt                                                                                                                >  2:6046135/1‑150 (MQ=255)
                                aTGGCGTCTGCGCGGTGAGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACt                                                                                                                <  2:4287928/150‑1 (MQ=255)
                                  ggCGTCTGCGCGGTGAGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTcc                                                                                                              >  2:10535222/1‑150 (MQ=255)
                                  ggCGTCTGCGCGGTGAGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTcc                                                                                                              <  1:5129499/150‑1 (MQ=255)
                                    cGTCTGCGCGGTGAGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAg                                                                                                            >  1:8090462/1‑150 (MQ=255)
                                      tCTGCGCGGTGAGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGcc                                                                                                          <  2:3749738/150‑1 (MQ=255)
                                         gcgcGGTGAGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCccgc                                                                                                       <  1:7059852/150‑1 (MQ=255)
                                           gcgGTGAGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCccgccg                                                                                                     >  2:3841469/1‑150 (MQ=255)
                                               tGAGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCt                                                                                                 >  2:8913100/1‑150 (MQ=255)
                                                 aGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTg                                                                                               >  2:1147445/1‑150 (MQ=255)
                                                 aGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTg                                                                                               <  1:1023381/150‑1 (MQ=255)
                                                 aGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTg                                                                                               >  1:9006967/1‑150 (MQ=255)
                                                      aTGGATGATCTTCTGCCCCAGGCGGGTAATGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTa                                                                                          >  1:2075882/1‑150 (MQ=255)
                                                       tGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAg                                                                                         <  1:7607117/150‑1 (MQ=255)
                                                        ggATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGc                                                                                        >  2:6628146/1‑150 (MQ=255)
                                                          atgatCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCcg                                                                                      >  2:1633103/1‑150 (MQ=255)
                                                          atgatCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCcg                                                                                      >  1:1141891/1‑150 (MQ=255)
                                                           tgatCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCcga                                                                                     <  2:7296072/150‑1 (MQ=255)
                                                                 tctGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGa                                                                               <  1:57771/150‑1 (MQ=255)
                                                                 tctGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGa                                                                               <  1:2674472/150‑1 (MQ=255)
                                                                  ctGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGAc                                                                              >  2:8733999/1‑150 (MQ=255)
                                                                  ctGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCAGGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGAc                                                                              <  1:5237184/150‑1 (MQ=255)
                                                                   tGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACg                                                                             >  1:7961844/1‑150 (MQ=255)
                                                                    gCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGa                                                                            <  2:7881831/150‑1 (MQ=255)
                                                                     ccccAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGaa                                                                           >  1:5609519/1‑150 (MQ=255)
                                                                       ccAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGt                                                                         >  2:6449889/1‑150 (MQ=255)
                                                                         aGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTcc                                                                       <  1:1251930/150‑1 (MQ=255)
                                                                         aGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTcc                                                                       <  2:5702872/150‑1 (MQ=255)
                                                                         aGGCGGGTAAAGAACTGCCCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTcc                                                                       <  1:8586176/150‑1 (MQ=255)
                                                                          ggCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCg                                                                      >  2:5882559/1‑150 (MQ=255)
                                                                           gCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCgg                                                                     <  2:9094639/150‑1 (MQ=255)
                                                                            cGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGa                                                                    <  2:10255161/150‑1 (MQ=255)
                                                                            cGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGa                                                                    <  1:1578686/150‑1 (MQ=255)
                                                                             gggTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGAt                                                                   <  1:7809001/150‑1 (MQ=255)
                                                                             gggTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGAt                                                                   >  2:9361626/1‑150 (MQ=255)
                                                                               gTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCg                                                                 >  2:8250025/1‑150 (MQ=255)
                                                                                tAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGc                                                                <  2:4462099/150‑1 (MQ=255)
                                                                                tAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGc                                                                <  2:745394/150‑1 (MQ=255)
                                                                                  aaGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGGCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCAc                                                              <  1:8715411/150‑1 (MQ=255)
                                                                                  aaGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCAc                                                              >  1:7348139/1‑150 (MQ=255)
                                                                                    taaCTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACgg                                                            >  2:3320420/2‑150 (MQ=255)
                                                                                    gAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACgg                                                            >  1:9976446/1‑150 (MQ=255)
                                                                                    gAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACgg                                                            >  1:6054661/1‑150 (MQ=255)
                                                                                    gAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACgg                                                            <  2:8616539/150‑1 (MQ=255)
                                                                                    gAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACgg                                                            >  1:3885251/1‑150 (MQ=255)
                                                                                     aaCTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGc                                                           <  1:4113553/150‑1 (MQ=255)
                                                                                        tGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTg                                                        >  1:1790475/1‑150 (MQ=255)
                                                                                         gcgcgcCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCTCGGCGTGc                                                       >  1:10659385/1‑150 (MQ=255)
                                                                                             gcCATCGATGGATTTGCCGCCATCGGTCTCGCCGTGGGGGTCGCCGTCATGGATGACCCCCAGGTCGAGGTCCGCGCCATGCCCGAACTCCAGCCCGCCGACCTCGCCGTAGCCGACGATGACGAAGTCCGGAGCGCACGGCGTGCCGTc                                                   <  2:1375105/150‑1 (MQ=255)
                                                                                              ccATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCg                                                  >  1:3375378/1‑150 (MQ=255)
                                                                                              ccATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCg                                                  >  1:3800061/1‑150 (MQ=255)
                                                                                                aTCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGgc                                                >  1:3523429/1‑150 (MQ=255)
                                                                                                 tCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGgcg                                               >  2:463618/1‑150 (MQ=255)
                                                                                                  cGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGgcgc                                              >  2:8131101/1‑150 (MQ=255)
                                                                                                   gATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGgcgcg                                             >  1:4195084/1‑150 (MQ=255)
                                                                                                    aTGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGgcgcgc                                            >  1:8839132/1‑150 (MQ=255)
                                                                                                     tGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCa                                           >  1:441789/1‑150 (MQ=255)
                                                                                                       gATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAAc                                         >  1:7160889/1‑150 (MQ=255)
                                                                                                            gccgccATCGGTCTCGCAGTGGGGGTCGCCGTCAGGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCTTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAAcggccg                                    <  1:3118018/150‑1 (MQ=255)
                                                                                                              cgccATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAAcggccggc                                  >  2:3589239/1‑150 (MQ=255)
                                                                                                              cgccATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAAcggccggc                                  >  1:2775599/1‑150 (MQ=255)
                                                                                                                ccATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGgccggccg                                >  2:6696640/1‑150 (MQ=255)
                                                                                                                ccATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGgccggccg                                >  2:2671114/1‑150 (MQ=255)
                                                                                                                ccATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGgccggccg                                >  1:1346006/1‑150 (MQ=255)
                                                                                                                ccATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGgccggccg                                <  1:6114270/150‑1 (MQ=255)
                                                                                                                  aTCGGTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGGCCGGCCGTg                              <  1:7435322/150‑1 (MQ=255)
                                                                                                                     ggTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGGCCGGCCGTGACg                           >  2:2321108/1‑150 (MQ=255)
                                                                                                                      gTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGGCCGGCCGGGACGc                          >  2:8078001/1‑150 (MQ=255)
                                                                                                                      gTCTCGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTCGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGGCCGGCCGTGACGc                          >  1:2452809/1‑150 (MQ=255)
                                                                                                                         tcGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGGCCGGCCGTGACGCTGc                       >  2:5328570/1‑150 (MQ=255)
                                                                                                                         tcGCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGGCCGGCCGTGACGCTGc                       >  1:9880053/1‑150 (MQ=255)
                                                                                                                           gCAGTGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGGCCGGCCGTGACGCTGCAc                     >  1:4472525/1‑150 (MQ=255)
                                                                                                                               tGGGGGTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGGCCGGCCGTGACGCTGCACCAAc                 <  2:279055/150‑1 (MQ=255)
                                                                                                                                   ggTCGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGGCCGGCCGTGACGCTGCACCAACTgcc             >  1:4944987/1‑150 (MQ=255)
                                                                                                                                      cGCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGGCCGGCCGTGACGCTGCACCAACTgccgcc          >  2:886280/1‑150 (MQ=255)
                                                                                                                                       gCCGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGGCCGGCCGTGACGCTGCACCAACTGCCgcca         >  2:10013094/1‑150 (MQ=255)
                                                                                                                                        ccGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGGCCGGCCGTGACGCTGCACCAACTGCCgccag        >  2:5184250/1‑150 (MQ=255)
                                                                                                                                         cGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGGCCGGCCGTGACGCTGCACCAACTGCCgccagg       >  1:6603127/1‑150 (MQ=255)
                                                                                                                                         cGTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGGCCGGCCGTGACGCTGCACCAACTGCCgccagg       >  2:7107326/1‑150 (MQ=255)
                                                                                                                                          gTCATGGATGTCCACCTGGTCGAGGTCGGAGCCATTACCGAACTCCAGCCCGCCGACCTTGCCTTAGACGACGATGACGAAGTCCGGATCGCTCGGCGTGCCTTCGGCGCGCAACGGCCGGCCGTGACGCTGCACCAACTGCCgcctggc      <  2:2978090/150‑1 (MQ=255)
                                                                                                                                          gTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGGCCGGCCGTGACGCTGCACCAACTGCCgccaggc      <  1:5532402/150‑1 (MQ=255)
                                                                                                                                          gTCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGGCCGGCCGTGACGCTGCACCAACTGCCgccaggc      >  1:5329534/1‑150 (MQ=255)
                                                                                                                                           tCATGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGGCCGGCCGTGACGCTGCACCAACTGCCgccaggcc     >  1:6747183/1‑150 (MQ=255)
                                                                                                                                              tGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGGCCGGCCGTGACGCTGCACCAACTGCCGCCAGGCCAGt  <  1:10454955/150‑1 (MQ=255)
                                                                                                                                              tGGATGAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGGCCGGCCGTGACGCTGCACCAACTGCCGCCAGGCCAGt  >  1:950547/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
CAGGCGCATGTCGACCTCGTAGAGGGTGCCGGATGGCGTCTGCGCGGTGAGGAAATGGATGATCTTCTGCCCCAGGCGGGTAAAGAACTGCGCGCCATCGATGGATTTGCCGCCATCGGTCTCGCAGTGGGGGTCGCCGTCATGGATAAACACCAGGTCGAGGTCCGAGCCATGACCGAACTCCAGCCCGCCGACCTTGCCGTAGCCGACGATGACGAAGTCCGGATCGCACGGCGTGCCGTCGGCGCGCAACGGCCGGCCGTGACGCTGCACCAACTGCCGCCAGGCCAGT  >  NC_008463/5904932‑5905223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑