Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 5,968,025 C→G P426A (CCC→GCC)  phaC → class II poly(R)‑hydroxyalkanoic acid synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084635,968,0250CG100.0% 1572.1 / NA 401P426A (CCC→GCC) phaCclass II poly(R)‑hydroxyalkanoic acid synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (190/211);  total (190/211)

Only 100 of 405 total aligned reads displayed.

AACTACTGGGTCAACAACTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCCCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGCGAGTTCATCCTC  >  NC_008463/5967878‑5968171
                                                                                                                                                   |                                                                                                                                                  
aaCTACTGGGTCAACAACTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGcgcc                                                                                                                                                  <  1:868704/150‑1 (MQ=255)
 actactGGGTCAACAACTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCTCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGcgccc                                                                                                                                                 <  2:3715913/150‑1 (MQ=255)
  ctactGGGTCAACAACTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCt                                                                                                                                                <  1:6495669/150‑1 (MQ=255)
   tactGGGTCAACAACTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTg                                                                                                                                               >  1:10044334/1‑150 (MQ=255)
      tGGGTCAACAACTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAg                                                                                                                                            >  2:3778936/1‑150 (MQ=255)
            aacaacTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTcc                                                                                                                                      <  2:8443853/150‑1 (MQ=255)
             acaacTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCg                                                                                                                                     <  1:463426/150‑1 (MQ=255)
              caacTACCTGCTCGGCAACCAGCAGCCGGCGTTCGTCATCCTCTACTGGAACAAAGACACCTCGCGCCTGCCCGCCGCGCTGCACGTCGAGTTCGTCGAAATGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCgg                                                                                                                                    <  2:229263/150‑1 (MQ=255)
               aacTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGc                                                                                                                                   >  1:5098128/1‑150 (MQ=255)
                acTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCa                                                                                                                                  <  1:6150675/150‑1 (MQ=255)
                  tACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACg                                                                                                                                >  2:10313526/1‑150 (MQ=255)
                   aCCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCAGCACGGCGAGATCGTCGAACAGTTCAAGAGCAACACGCTGAACCGCCGCGGCGCCCTGGAGGTCTCCGGCACGc                                                                                                                               >  2:2946625/1‑150 (MQ=25)
                    ccTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGcc                                                                                                                              >  2:10581980/1‑150 (MQ=255)
                     cTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGccc                                                                                                                             >  1:3540094/1‑150 (MQ=255)
                      tGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCa                                                                                                                            <  2:7305111/150‑1 (MQ=255)
                      agCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCa                                                                                                                            <  1:9366128/149‑1 (MQ=255)
                       gCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCAt                                                                                                                           >  2:10187131/1‑150 (MQ=255)
                            gCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGAcc                                                                                                                      >  1:3397350/1‑150 (MQ=255)
                             cAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCt                                                                                                                     <  1:8989244/150‑1 (MQ=255)
                               aCCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGa                                                                                                                   >  2:513617/1‑150 (MQ=255)
                                ccAGCCGCCGTCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGTGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGaa                                                                                                                  <  2:177336/150‑1 (MQ=255)
                                ccAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGaa                                                                                                                  <  1:5717077/150‑1 (MQ=255)
                                      gccgGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGt                                                                                                            >  2:10395505/1‑150 (MQ=255)
                                       ccgGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCAGCGACCTGAAGCAGGTg                                                                                                           >  2:7595189/1‑150 (MQ=255)
                                        cgGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGa                                                                                                          >  1:6350922/1‑150 (MQ=255)
                                            gTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTg                                                                                                      <  1:5191171/150‑1 (MQ=255)
                                            gTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTg                                                                                                      <  2:85985/150‑1 (MQ=255)
                                             ttCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGc                                                                                                     <  1:6116109/150‑1 (MQ=255)
                                             ttCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGc                                                                                                     <  2:5299303/150‑1 (MQ=255)
                                               cGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGa                                                                                                   <  1:9922692/150‑1 (MQ=255)
                                                gACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGAc                                                                                                  <  1:2155963/150‑1 (MQ=255)
                                                gACATCCTCTACTGGAACAACGACACCACGCGCCAGCCCGCCGCGCTGCACGGCGAGTTCGACGAACTGTACAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGAc                                                                                                  >  2:1266473/1‑150 (MQ=255)
                                                 aCATCCTCTACTGGAACAACGACACCACGCGCCGGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACt                                                                                                 <  2:9859877/150‑1 (MQ=255)
                                                      ctctACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTAc                                                                                            >  1:1744839/1‑150 (MQ=255)
                                                          aCTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTAGCGACTTCTACtgtg                                                                                        >  2:2815333/1‑150 (MQ=255)
                                                              gAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGc                                                                                    <  1:10581235/150‑1 (MQ=255)
                                                              gAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGc                                                                                    <  1:9564017/150‑1 (MQ=255)
                                                                acaacGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCg                                                                                  <  2:8525920/150‑1 (MQ=255)
                                                                acaacGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCg                                                                                  <  1:1924620/150‑1 (MQ=255)
                                                                 caacGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCgg                                                                                 >  1:2731889/1‑150 (MQ=255)
                                                                 caacGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCgg                                                                                 <  2:10462688/150‑1 (MQ=255)
                                                                   acGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTc                                                                               >  1:3629959/1‑150 (MQ=255)
                                                                   acGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTc                                                                               >  1:4781115/1‑150 (MQ=255)
                                                                    cGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCt                                                                              <  1:757429/150‑1 (MQ=255)
                                                                      acacCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGa                                                                            >  2:5713933/1‑150 (MQ=255)
                                                                      acacCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGa                                                                            <  2:7860307/150‑1 (MQ=255)
                                                                         ccacGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACg                                                                         >  2:6302077/1‑150 (MQ=255)
                                                                         ccacGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACg                                                                         <  1:863975/150‑1 (MQ=255)
                                                                          cacGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGa                                                                        >  2:6442616/1‑150 (MQ=255)
                                                                           acGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGAc                                                                       >  1:2919633/1‑150 (MQ=255)
                                                                            cgcgCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCCCGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACCGTGTCGCCGGTCTGAACGAcc                                                                      <  2:244971/150‑1 (MQ=255)
                                                                            cgcgCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGAcc                                                                      >  2:2006683/1‑150 (MQ=255)
                                                                                ccTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACAt                                                                  >  2:7137550/1‑150 (MQ=255)
                                                                                ccTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACAt                                                                  >  2:3298133/1‑150 (MQ=255)
                                                                                 cTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATc                                                                 <  1:4229972/150‑1 (MQ=255)
                                                                                 cTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATc                                                                 <  1:10695670/150‑1 (MQ=255)
                                                                                  tGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCa                                                                >  1:1775437/1‑150 (MQ=255)
                                                                                   gCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCAc                                                               <  2:9186868/150‑1 (MQ=255)
                                                                                    cccGCCGCGCTGCACGGCGAGTTCGTCGAACAGTTCAATAGCAACCCGCAGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCAcc                                                              >  2:5437320/1‑150 (MQ=255)
                                                                                            gcTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGa                                                      >  2:3956305/1‑150 (MQ=255)
                                                                                             cTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAg                                                     >  2:148340/1‑150 (MQ=255)
                                                                                             cTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAg                                                     >  1:2333604/1‑150 (MQ=255)
                                                                                              tGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGt                                                    <  2:1961718/150‑1 (MQ=255)
                                                                                              tGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGt                                                    >  2:4451543/1‑150 (MQ=255)
                                                                                              tGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGt                                                    >  2:4303631/1‑150 (MQ=255)
                                                                                                cACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCg                                                  <  1:1182991/150‑1 (MQ=255)
                                                                                                cACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCg                                                  <  1:78185/150‑1 (MQ=255)
                                                                                                 aCGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGt                                                 >  2:9007420/1‑150 (MQ=255)
                                                                                                   ggCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGc                                               <  2:9540173/150‑1 (MQ=255)
                                                                                                    gCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCt                                              <  1:3280394/150‑1 (MQ=255)
                                                                                                    gCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCt                                              >  2:6805325/1‑150 (MQ=255)
                                                                                                    gCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCt                                              <  2:1702943/150‑1 (MQ=255)
                                                                                                        gTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACaa                                          >  1:3944676/1‑150 (MQ=255)
                                                                                                          tcgtcgAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGt                                        <  1:5399215/150‑1 (MQ=255)
                                                                                                           cgtcgAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTc                                       <  1:7969667/150‑1 (MQ=255)
                                                                                                            gtcgAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCg                                      <  1:6728948/150‑1 (MQ=255)
                                                                                                             tcgAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCgg                                     >  1:5916756/1‑150 (MQ=255)
                                                                                                              cgAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGc                                    >  2:8228002/1‑150 (MQ=255)
                                                                                                                aaCTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCa                                  <  1:7353594/150‑1 (MQ=255)
                                                                                                                aaCTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCa                                  >  2:4142591/1‑150 (MQ=255)
                                                                                                                   tGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGACCGACCACATCACCCCCTGGGAGTCGTGCTCCAAGTCGGCCAGGc                               >  2:4513366/1‑150 (MQ=255)
                                                                                                                   tGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGc                               <  1:5843040/150‑1 (MQ=255)
                                                                                                                    gTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCt                              <  1:1823498/150‑1 (MQ=255)
                                                                                                                       cAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACt                           <  2:6098225/150‑1 (MQ=255)
                                                                                                                             cAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGggcgg                     <  1:909254/150‑1 (MQ=255)
                                                                                                                              aaCCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGggcggc                    <  1:3840797/150‑1 (MQ=255)
                                                                                                                                cccGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCaa                  <  2:700716/150‑1 (MQ=255)
                                                                                                                                cccGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCaa                  >  1:8872329/1‑150 (MQ=255)
                                                                                                                                 ccGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAg                 <  1:4031775/150‑1 (MQ=255)
                                                                                                                                   gCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTg               >  2:5252611/1‑150 (MQ=255)
                                                                                                                                    cTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGc              >  2:3640028/1‑150 (MQ=255)
                                                                                                                                    cTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGc              >  2:5907806/1‑150 (MQ=255)
                                                                                                                                        aCCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGCGAGt          <  2:10099008/150‑1 (MQ=255)
                                                                                                                                        aCCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGCGAGt          <  1:9752468/150‑1 (MQ=255)
                                                                                                                                          cGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGCGAGTTc        <  2:4897928/150‑1 (MQ=255)
                                                                                                                                              ccGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGGCGGCCCGGCTACTGGGCGGCAAGTGCGAGTTCATcc    >  2:2752472/1‑150 (MQ=255)
                                                                                                                                               cGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGCTAGTTCATCct   >  2:2993626/1‑150 (MQ=255)
                                                                                                                                               cGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGCGAGTTCATCct   <  2:1106469/150‑1 (MQ=255)
                                                                                                                                                ggCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGCGAGTTCATCctc  <  2:4992582/150‑1 (MQ=255)
                                                                                                                                                ggCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGCGAGTTCATCctc  <  1:8340799/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AACTACTGGGTCAACAACTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCCCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGCGAGTTCATCCTC  >  NC_008463/5967878‑5968171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑