Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 72,440 T→C K132E (AAG→GAG)  PA14_RS00315 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00846372,4400TC99.2% 1573.8 / NA 400K132E (AAG→GAG) PA14_RS00315hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (150/247);  minor base A (1/2);  total (151/249)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 401 total aligned reads displayed.

GGCAGCGGTCCGGCGCAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTTGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAATCGCGCCAGAGCGGGC  >  NC_008463/72293‑72586
                                                                                                                                                   |                                                                                                                                                  
 gCAGCGGTCCGGCGCAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTcgcg                                                                                                                                                 >  1:7997786/1‑150 (MQ=255)
   aGCGGTCCGGCGCAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGc                                                                                                                                               <  1:3330204/150‑1 (MQ=255)
    gCGGTCCGGCGCAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGcc                                                                                                                                              <  1:10231115/150‑1 (MQ=255)
    gCGGTCCGGCGCAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGcc                                                                                                                                              >  1:9581699/1‑150 (MQ=255)
       gTCCGGCGCAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCcgcg                                                                                                                                           >  1:9161208/1‑150 (MQ=255)
        tCCGGCGCAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCcgcgc                                                                                                                                          <  2:7094240/150‑1 (MQ=255)
        tCCGGCGCAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCcgcgc                                                                                                                                          >  1:1965534/1‑150 (MQ=255)
         ccGGCGCAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCcgcgcg                                                                                                                                         >  1:4196779/1‑150 (MQ=255)
           ggCGCAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGcc                                                                                                                                       <  2:5696019/150‑1 (MQ=255)
              gcAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAAt                                                                                                                                    <  2:6179/150‑1 (MQ=255)
                aGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTc                                                                                                                                  <  1:5348886/150‑1 (MQ=255)
                  ccGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTccc                                                                                                                                <  1:5109441/150‑1 (MQ=255)
                   cGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCt                                                                                                                               >  2:150061/1‑150 (MQ=255)
                   cGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCt                                                                                                                               <  1:9637186/150‑1 (MQ=255)
                   cGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCt                                                                                                                               <  1:2518095/150‑1 (MQ=255)
                     gCGAGGAGCAGCAGCGCGTGCGGGACGAGACGGGTGAGTGGACGCAGGGGGCGGGCTCCGGAGGGCGTGCAGCTCCTAGCATAGCGCCCTCGCCTAGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCtgt                                                                                                                             >  2:8716859/1‑150 (MQ=255)
                     gCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGTGTGGACGCATGGGGCTGGCTCCGGAGGGCGTTCAGCTACTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCTATTCCCtgt                                                                                                                             <  2:751995/150‑1 (MQ=255)
                     gCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCtgt                                                                                                                             >  1:2276343/1‑150 (MQ=255)
                       gaggagCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCACTTCCCtgttg                                                                                                                           >  2:1004008/1‑150 (MQ=255)
                       gaggagCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCtgttg                                                                                                                           >  2:4664260/1‑150 (MQ=255)
                        aggagCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCtgttgt                                                                                                                          >  1:951975/1‑150 (MQ=255)
                               gcagcGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTc                                                                                                                   <  1:1441813/150‑1 (MQ=255)
                                cagcGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCg                                                                                                                  <  2:10708313/150‑1 (MQ=255)
                                cagcGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCg                                                                                                                  >  1:7244913/1‑150 (MQ=255)
                                  gcgcGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTCGTCCTGGTCGTa                                                                                                                <  2:6762517/150‑1 (MQ=255)
                                    gcgAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTaga                                                                                                              <  1:4437491/150‑1 (MQ=255)
                                     cgAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTagag                                                                                                             >  2:9680802/1‑150 (MQ=255)
                                     cgAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTagag                                                                                                             >  2:4282500/1‑150 (MQ=255)
                                      gAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGc                                                                                                            <  2:9369679/150‑1 (MQ=255)
                                       aGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCt                                                                                                           >  2:9042213/1‑150 (MQ=255)
                                        gCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCtt                                                                                                          <  2:8465626/150‑1 (MQ=255)
                                        gCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCtt                                                                                                          <  2:1817977/150‑1 (MQ=255)
                                             aCGAGACGGGTGAGTGGACGCCTGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCATCGCCTTGCGCCGTTCAGACCTGCGCGCAGCGCCCCTCGCGGCAGCGCGCCAATTCCCTGGTGTCCTGGTCGTATAGCTTCGccc                                                                                                     >  2:4000504/1‑150 (MQ=255)
                                             aCGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGccc                                                                                                     <  1:5230596/150‑1 (MQ=255)
                                                agaCGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAgg                                                                                                  >  1:5629941/1‑150 (MQ=255)
                                                 gaCGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAggg                                                                                                 >  1:10729529/1‑150 (MQ=255)
                                                 gaCGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAggg                                                                                                 <  1:1734949/150‑1 (MQ=255)
                                                  aCGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGa                                                                                                <  2:5374092/150‑1 (MQ=255)
                                                  aCGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGa                                                                                                <  2:1702346/150‑1 (MQ=255)
                                                    gggTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCACTTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACg                                                                                              <  2:3078140/150‑1 (MQ=255)
                                                      gTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGa                                                                                            <  1:3515098/150‑1 (MQ=255)
                                                      gTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGa                                                                                            >  2:10532798/1‑150 (MQ=255)
                                                        gAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAg                                                                                          <  2:2120402/150‑1 (MQ=255)
                                                          gTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGcc                                                                                        >  2:3243284/1‑150 (MQ=255)
                                                          gTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGcc                                                                                        <  2:10207237/150‑1 (MQ=255)
                                                           tGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCg                                                                                       <  1:5694922/150‑1 (MQ=255)
                                                             gACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCAGAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGAt                                                                                     <  2:6480500/150‑1 (MQ=255)
                                                              aCGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGAtg                                                                                    <  2:8190222/150‑1 (MQ=255)
                                                              aCGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGAtg                                                                                    <  1:6344473/150‑1 (MQ=255)
                                                                  aTGGGGCGGGCTCCGGAGGGCGTACAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGcc                                                                                <  1:863320/150‑1 (MQ=255)
                                                                   tGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGccc                                                                               >  1:6964536/1‑150 (MQ=255)
                                                                     gggagggcTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCgg                                                                             >  1:3725517/1‑150 (MQ=255)
                                                                      ggcgggcTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGc                                                                            <  2:3590840/150‑1 (MQ=255)
                                                                       gcgggcTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGcc                                                                           <  1:3892962/150‑1 (MQ=255)
                                                                             tCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAgg                                                                     <  1:7712/150‑1 (MQ=255)
                                                                             tCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAgg                                                                     >  1:2890370/1‑150 (MQ=255)
                                                                               cGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCg                                                                   <  2:4936528/150‑1 (MQ=255)
                                                                                ggAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCAGGCCTCCAGGCgg                                                                  <  1:10383881/150‑1 (MQ=255)
                                                                                 gAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGt                                                                 >  1:1363309/1‑150 (MQ=255)
                                                                                 gAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGt                                                                 >  1:10041114/1‑150 (MQ=255)
                                                                                 gAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGt                                                                 >  1:10567761/1‑150 (MQ=255)
                                                                                 gAGGGCGTTCAGCTCCTAGCATAGCGACCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCTCCAATTCCCTGTTGTCCTGGTCGTAGAGCTACGCACAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGt                                                                 >  2:796895/1‑150 (MQ=255)
                                                                                 gAGGGCGTTCAACTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGt                                                                 <  2:5718735/150‑1 (MQ=255)
                                                                                   gggCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAg                                                               <  2:2895627/150‑1 (MQ=255)
                                                                                   gggCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAAATCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAg                                                               >  2:6444106/1‑150 (MQ=255)
                                                                                    ggCGTTCAGCTCCTAGCATTTCCCCCTCGCCGTGCGCCGTTCAGACCTTCGCGCAGCGCCCCACGCGGCCGCGCGCCACTTCCATGTGGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAgc                                                              <  2:2505210/150‑1 (MQ=255)
                                                                                     gCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCGCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAgcg                                                             >  2:7592986/1‑150 (MQ=255)
                                                                                       gTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCt                                                           >  2:3548048/1‑150 (MQ=255)
                                                                                         tCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTgg                                                         >  1:8857145/1‑150 (MQ=255)
                                                                                          cAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGCTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGc                                                        <  2:8586235/150‑1 (MQ=255)
                                                                                          cAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAAGTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGc                                                        <  2:8802206/150‑1 (MQ=255)
                                                                                             cTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCgg                                                     >  2:1280406/1‑150 (MQ=255)
                                                                                             cTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCgg                                                     >  1:2661287/1‑150 (MQ=255)
                                                                                                cTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGgcgc                                                  >  1:8240377/1‑150 (MQ=255)
                                                                                                cTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGgcgc                                                  >  1:8201344/1‑150 (MQ=255)
                                                                                                 tAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCt                                                 <  1:4601076/150‑1 (MQ=255)
                                                                                                  aGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTg                                                >  1:6724907/1‑150 (MQ=255)
                                                                                                      tAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGt                                            <  1:7836794/150‑1 (MQ=255)
                                                                                                           cccTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTg                                       <  1:8461668/150‑1 (MQ=255)
                                                                                                           cccTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTg                                       >  1:807036/1‑150 (MQ=255)
                                                                                                           cccTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTg                                       >  1:2204151/1‑150 (MQ=255)
                                                                                                            ccTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGa                                      <  1:9012616/150‑1 (MQ=255)
                                                                                                             cTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGaa                                     >  1:9855147/1‑150 (MQ=255)
                                                                                                             cTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGaa                                     <  2:6043916/150‑1 (MQ=255)
                                                                                                                gCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACtt                                  >  1:5917929/1‑150 (MQ=255)
                                                                                                                  cTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGa                                >  1:7097513/1‑150 (MQ=255)
                                                                                                                  cTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGa                                <  2:9297760/150‑1 (MQ=255)
                                                                                                                    tGCGCCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCACTTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAgg                              <  1:61315/150‑1 (MQ=255)
                                                                                                                      cgcCGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGtt                            <  2:3849466/150‑1 (MQ=255)
                                                                                                                        ccGTTCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGa                          <  2:8386097/150‑1 (MQ=255)
                                                                                                                            tCAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTg                      <  2:2772820/150‑1 (MQ=255)
                                                                                                                             cAGACCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTgg                     <  1:9248944/150‑1 (MQ=255)
                                                                                                                                aCCTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCaa                  >  1:10695910/1‑150 (MQ=255)
                                                                                                                                 ccTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAAt                 <  1:2799181/150‑1 (MQ=255)
                                                                                                                                 ccTTCGCGCAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGGTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAAt                 <  2:8922998/150‑1 (MQ=255)
                                                                                                                                        gcAGCGCCCCTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAATCGCGCCa          <  1:9961401/150‑1 (MQ=255)
                                                                                                                                           gcgcCCCTCGCGGCCGCGCGCCAATGCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCCGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCACTCGCGCCagag       >  1:1257661/1‑150 (MQ=255)
                                                                                                                                              ccccTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAATCGCGCCAGAGCgg    >  1:1497530/1‑150 (MQ=255)
                                                                                                                                                ccTCGCGGCCGCGCGCCAATTCCCTGTTTTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACGTGAGGTTGAGCTGGCAATCGCGCCAGAGCGGGc  >  1:3137980/1‑150 (MQ=255)
                                                                                                                                                ccTCGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGTGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAATCGCGCCAGAGCGGGc  <  2:2082105/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGCAGCGGTCCGGCGCAGCCGGCGAGGAGCAGCAGCGCGAGCGGGACGAGACGGGTGAGTGGACGCATGGGGCGGGCTCCGGAGGGCGTTCAGCTCCTAGCATAGCGCCCTCGCCTTGCGCCGTTCAGACCTTCGCGCAGCGCCCCTTGCGGCCGCGCGCCAATTCCCTGTTGTCCTGGTCGTAGAGCTTCGCCCAGGGACGGAAGCCGATGTGCCCGGCCTCCAGGCGGTAGCGCTGGCCGGCGCTGAAGTCCTTGAACTTGAGGTTGAGCTGGCAATCGCGCCAGAGCGGGC  >  NC_008463/72293‑72586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑