Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 1,558,205 A→G Q390Q (CAA→CAG)  PA14_RS07290 → propionyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084631,558,2050AG100.0% 1759.6 / NA 443Q390Q (CAA→CAG) PA14_RS07290propionyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (225/218);  total (225/218)

Only 100 of 443 total aligned reads displayed.

CAAGGAAGACCCGCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAAACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCGCTGGGAGCGAACCGCCAGGG  >  NC_008463/1558058‑1558351
                                                                                                                                                   |                                                                                                                                                  
 aaGGAAGACCCGCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGAcc                                                                                                                                                 <  2:704932/150‑1 (MQ=255)
 aaGGAAGACCCGCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGAcc                                                                                                                                                 >  1:3272716/1‑150 (MQ=255)
       gACCCGCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCAGTGGTGGCAGACCGAGAcc                                                                                                                                           >  1:1251806/1‑150 (MQ=255)
           cGCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCt                                                                                                                                       >  1:2675318/1‑150 (MQ=255)
                ggCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCg                                                                                                                                  >  1:7720910/1‑150 (MQ=255)
                 gCGAACGGGAGAAGCGCAACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACGGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCgg                                                                                                                                 <  2:3054443/150‑1 (MQ=255)
                   gAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTg                                                                                                                               >  1:9435747/1‑150 (MQ=255)
                   gAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTg                                                                                                                               <  2:3904807/150‑1 (MQ=255)
                   gAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGACACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTCGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTg                                                                                                                               >  2:2122480/1‑150 (MQ=255)
                    aaCGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGa                                                                                                                              >  2:5831956/1‑150 (MQ=255)
                    aaCGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGa                                                                                                                              >  2:521131/1‑150 (MQ=255)
                     aCGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGAc                                                                                                                             >  2:7344794/1‑150 (MQ=255)
                     aCGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGAc                                                                                                                             >  1:6319152/1‑150 (MQ=255)
                     aCGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGAc                                                                                                                             <  1:462715/150‑1 (MQ=255)
                      cGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGAcc                                                                                                                            >  1:7566448/1‑150 (MQ=255)
                      cGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGAcc                                                                                                                            >  2:7941942/1‑150 (MQ=255)
                      cGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGAcc                                                                                                                            <  2:9241746/150‑1 (MQ=255)
                         gTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACcgcg                                                                                                                         >  1:2252753/1‑150 (MQ=255)
                          tGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCgcgc                                                                                                                        >  2:5818961/1‑150 (MQ=255)
                           gAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGcc                                                                                                                       <  1:2093645/150‑1 (MQ=255)
                            aaGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGccc                                                                                                                      >  2:7459843/1‑150 (MQ=255)
                              gcgcTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTg                                                                                                                    >  2:1710856/1‑150 (MQ=255)
                                gcTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCa                                                                                                                  <  1:10672123/150‑1 (MQ=255)
                                 cTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCAt                                                                                                                 >  1:6886597/1‑150 (MQ=255)
                                  tACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATc                                                                                                                <  2:339204/150‑1 (MQ=255)
                                  tACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATc                                                                                                                >  2:10464945/1‑150 (MQ=255)
                                   aCGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCg                                                                                                               <  2:9736840/150‑1 (MQ=255)
                                   aCGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCg                                                                                                               >  2:4536171/1‑150 (MQ=255)
                                      aCCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGa                                                                                                            <  1:2548013/150‑1 (MQ=255)
                                       ccTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGAt                                                                                                           <  1:8028084/150‑1 (MQ=255)
                                       ccTAAGTTCGCTGCGCCACCTGTTCCTCGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGAt                                                                                                           >  1:3749130/1‑150 (MQ=255)
                                        cTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATc                                                                                                          <  1:10036573/150‑1 (MQ=255)
                                         tAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCg                                                                                                         <  1:6486171/150‑1 (MQ=255)
                                          aaGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGc                                                                                                        >  1:1512758/1‑150 (MQ=255)
                                           aGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGcc                                                                                                       <  1:2787874/150‑1 (MQ=255)
                                           aGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGcc                                                                                                       >  1:7340514/1‑150 (MQ=255)
                                                gCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCa                                                                                                  >  2:1631537/1‑150 (MQ=255)
                                                 cTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCAc                                                                                                 <  2:8356036/150‑1 (MQ=255)
                                                    cgcCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGAc                                                                                              >  1:4343019/1‑150 (MQ=255)
                                                      ccACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCt                                                                                            <  1:3926149/150‑1 (MQ=255)
                                                          cTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCgg                                                                                        >  1:1059187/1‑150 (MQ=255)
                                                           tGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCggg                                                                                       <  1:3503505/150‑1 (MQ=255)
                                                               ccTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCgg                                                                                   <  1:4753711/150‑1 (MQ=255)
                                                                cTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGt                                                                                  <  2:5948898/150‑1 (MQ=255)
                                                                 tGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGtt                                                                                 <  2:2346978/150‑1 (MQ=255)
                                                                  ggCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTc                                                                                >  1:9585358/1‑150 (MQ=255)
                                                                   gCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCg                                                                               <  1:7954771/150‑1 (MQ=255)
                                                                   gCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCg                                                                               >  2:1931308/1‑150 (MQ=255)
                                                                      ggCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGAcc                                                                            >  2:7509461/1‑150 (MQ=255)
                                                                      ggCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGAcc                                                                            >  1:5320794/1‑150 (MQ=255)
                                                                          agaAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAAcc                                                                        <  2:3850387/150‑1 (MQ=255)
                                                                          agaAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAAcc                                                                        >  2:1460901/1‑150 (MQ=255)
                                                                              gCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACcgcgc                                                                    >  2:5553013/1‑150 (MQ=255)
                                                                              gCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACcgcgc                                                                    >  1:10169554/1‑150 (MQ=255)
                                                                               cTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACcgcgcg                                                                   <  2:1104873/150‑1 (MQ=255)
                                                                               cTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACcgcgcg                                                                   <  2:9825329/150‑1 (MQ=255)
                                                                                tCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCgg                                                                  <  2:8571858/150‑1 (MQ=255)
                                                                                 cGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGt                                                                 <  2:9544448/150‑1 (MQ=255)
                                                                                   aCAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGc                                                               >  2:7703568/1‑150 (MQ=255)
                                                                                    cagcagCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGcc                                                              <  2:6373664/150‑1 (MQ=255)
                                                                                    cagcagCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGcc                                                              >  1:5863147/1‑150 (MQ=255)
                                                                                     agcagcACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGccc                                                             <  1:8282927/150‑1 (MQ=255)
                                                                                        agcaCCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGc                                                          <  1:9196029/150‑1 (MQ=255)
                                                                                         gcaCCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCTTTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCt                                                         <  1:1106255/150‑1 (MQ=255)
                                                                                         gcaCCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCt                                                         <  2:3940964/150‑1 (MQ=255)
                                                                                          caCCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTa                                                        <  1:6818153/150‑1 (MQ=255)
                                                                                          caCCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTa                                                        <  1:1768915/150‑1 (MQ=255)
                                                                                           aCCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTAc                                                       >  2:4405301/1‑150 (MQ=255)
                                                                                             ccAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCa                                                     <  1:10298689/150‑1 (MQ=255)
                                                                                               aGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACg                                                   <  2:8818067/150‑1 (MQ=255)
                                                                                                  aCTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGc                                                >  1:3350716/1‑150 (MQ=255)
                                                                                                    tggctggAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAg                                              >  2:2676126/1‑150 (MQ=255)
                                                                                                     ggctggAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAgg                                             <  1:6083168/150‑1 (MQ=255)
                                                                                                     ggctggAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAgg                                             <  1:8924875/150‑1 (MQ=255)
                                                                                                     ggctggAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAgg                                             <  1:4834329/150‑1 (MQ=255)
                                                                                                      gctggAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCACCCGCGCGGTGCCCGGCTACCACGGGCAGGt                                            >  2:9171713/1‑150 (MQ=255)
                                                                                                      gctggAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGt                                            >  2:5087362/1‑150 (MQ=255)
                                                                                                      gctggAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGt                                            <  2:6689250/150‑1 (MQ=255)
                                                                                                       ctggAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTg                                           >  1:1643015/1‑150 (MQ=255)
                                                                                                          gAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTg                                        >  2:9366183/1‑150 (MQ=255)
                                                                                                           aaGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTgg                                       <  1:9638796/150‑1 (MQ=255)
                                                                                                           aaGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTgg                                       <  2:3287779/150‑1 (MQ=255)
                                                                                                             gagCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGgac                                     >  1:8524624/1‑150 (MQ=255)
                                                                                                             gagCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGgac                                     <  1:8097334/150‑1 (MQ=255)
                                                                                                                 acacCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGacgacg                                 <  1:4023472/150‑1 (MQ=255)
                                                                                                                   acCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGaa                               <  2:6193781/150‑1 (MQ=255)
                                                                                                                    ccGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAg                              >  1:3091901/1‑150 (MQ=255)
                                                                                                                     cGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAgg                             <  2:6886597/150‑1 (MQ=255)
                                                                                                                     cGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAgg                             >  1:6654162/1‑150 (MQ=255)
                                                                                                                       ggcggcCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTCCCACGTGCAGGTGCTGGACGACGAAggac                           >  2:4197070/1‑148 (MQ=255)
                                                                                                                        gcggcCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAggccg                          >  1:2501640/1‑150 (MQ=255)
                                                                                                                         cggcCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAggccgg                         <  1:9891520/150‑1 (MQ=255)
                                                                                                                                tCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCGCTgg                  <  2:3646438/150‑1 (MQ=255)
                                                                                                                                 ccACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCGCTggg                 >  2:7640370/1‑150 (MQ=255)
                                                                                                                                  cACGACCATTGGTGGCAGTCCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCGGGACGACGAAGGCCGGCCGCGGGGa                >  2:889993/1‑150 (MQ=255)
                                                                                                                                  cACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCGCTGGGa                >  1:9761571/1‑150 (MQ=255)
                                                                                                                                   aCGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCGCTGGGAg               >  1:5133794/1‑150 (MQ=255)
                                                                                                                                        cATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCGCTGGGAGCGAAc          <  1:5693007/150‑1 (MQ=255)
                                                                                                                                            ggtggCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCGCTGGGAGCGAACCGcc      <  1:7829749/150‑1 (MQ=255)
                                                                                                                                             gtggCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCGCTGGGAGCGAACCGCCa     >  1:7111447/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CAAGGAAGACCCGCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAAACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCGCTGGGAGCGAACCGCCAGGG  >  NC_008463/1558058‑1558351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑