BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	2,001,811	A→C	Q66P (CAG→CCG)	pgl →	6‑phosphogluconolactonase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	2,001,811	0	A	C	100.0%	1657.5 / NA	419	Q66P (CAG→CCG)	pgl	6‑phosphogluconolactonase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (198/221); total (198/221)

Only 100 of 419 total aligned reads displayed.

CAGCGCCGCCGAGCAGGCCCGCGGCCTGGCCGCCGAGGTCGCCGGCCGGTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGATCAGGTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAGGCGGCGAAGGCGCGCTTCATCGGCCTCTACCAG  >  NC_008463/2001668‑2001953

 aGCGCCGCCGAGCAGGCCCGCGGCCTGGCCGCCGAGGTCGCCGGCCGGTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGAc                                                                                                                                         <  1:4140439/150‑1 (MQ=255)

     ccgccgAGCAGGCCCGCGGCCTGGCCGCCGAGGTCGCCGGCCGGTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTggg                                                                                                                                     >  2:1476119/1‑149 (MQ=255)

     ccgccgAGCAGGCCCGCGGCCTGGCCGCCGAGGTCGCCGGCCGGTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGt                                                                                                                                     <  1:5546832/150‑1 (MQ=255)

      cgccgAGCAGGCCCGCGGCCTGGCCGCCGAGGTCGCCGGCCGGTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTc                                                                                                                                    >  1:8715450/1‑150 (MQ=255)

       gccgAGCAGGCCCGCGGCCTGGCCGCCGAGGTCGCCGGCCGGTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTcg                                                                                                                                   >  1:5862884/1‑150 (MQ=255)

        ccgAGCAGGCCCGCGGCCTGGCCGCCGAGGTCGCCGGCCGGTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTcgc                                                                                                                                  >  1:4510627/1‑150 (MQ=255)

         cgAGCAGGCCCGCGGCCTGGCCGCCGAGGTCGCCGGCCGGTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTcgcg                                                                                                                                 <  1:9998621/150‑1 (MQ=255)

         cgAGCAGGCCCGCGGCCTGGCCGCCGAGGTCGCCGGCCGGTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTcgcg                                                                                                                                 <  1:4870739/150‑1 (MQ=255)

           aGCAGGCCCGCGGCCTGGCCGCCGAGGTCGCCGGCCGGTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGa                                                                                                                               >  1:7455361/1‑150 (MQ=255)

                gCCCGCGGCCTGGCCGCCGACGACGCCGGCCGGCTGCGCTCGGCCCTGGCCGAGCAGGGCCAGGCGCTGCTCGTCGTGTCCGGTGGGCGCAGTCCGGGGGCCTTCCTCGAAGCCTTGCGCGAGGAGCCGCTGGACGGGTCGCGGATCAcc                                                                                                                          <  2:7656760/150‑1 (MQ=255)

                      ggCCTGGCCGCCGAGGTCGCCGGCCGGTTGCGCTCGGCGCGGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGc                                                                                                                    <  2:685226/150‑1 (MQ=255)

                       gCCTGGCCGCCGAGGTCGCCGGCCGGTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGcc                                                                                                                   >  2:3344389/1‑150 (MQ=255)

                          tGGCCGCCGAGGTCGCCGGCCGGTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTgg                                                                                                                <  2:2913495/150‑1 (MQ=255)

                          tGGCCGCCGAGGTCGCCGGCCGGTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTgg                                                                                                                >  1:4334714/1‑150 (MQ=255)

                           gtccgccgAGGTCGGCGGCAGGTTGCTCTCGGAGCTGGCCGAGCAGGGCCAGGCGCGGCTGGTGGGGTCAGGTGGGCGCAGTCCGGTGGCCTTCCTCGTAGCCTTGTGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGc                                                                                                               <  2:423440/148‑1 (MQ=255)

                              cgccgAGGTCGCCGGCCGGTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCcga                                                                                                            <  1:6613564/150‑1 (MQ=255)

                                    ggTCGCCGGCCGGTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTTGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAgcg                                                                                                      <  2:2974094/150‑1 (MQ=255)

                                    ggTCGCCGGCCGGTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAgcg                                                                                                      >  2:3025539/1‑150 (MQ=255)

                                     gTCGCCGGCCGGTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAgcgc                                                                                                     >  2:850657/1‑150 (MQ=255)

                                      tCGCCGGCCGGTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCt                                                                                                    <  2:4775112/150‑1 (MQ=255)

                                      tCGCCGGCCGGGTGCGCTCGGCGCGGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCt                                                                                                    <  2:2452247/150‑1 (MQ=255)

                                      gcGCCGGCCGGTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCt                                                                                                    >  1:3722381/2‑150 (MQ=255)

                                       cGCCGGCCGGTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTg                                                                                                   >  2:8098055/1‑150 (MQ=255)

                                        gccggccgGTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTgg                                                                                                  >  1:2605974/1‑150 (MQ=255)

                                         ccggccggTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTggg                                                                                                 >  1:3172270/1‑150 (MQ=255)

                                            gccggTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGTGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGc                                                                                              >  2:9043610/1‑150 (MQ=255)

                                             ccggTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGcc                                                                                             <  2:6277047/150‑1 (MQ=255)

                                               ggTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCgg                                                                                           >  1:3375451/1‑150 (MQ=255)

                                                gTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGa                                                                                          <  1:6520461/150‑1 (MQ=255)

                                                 ttGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAg                                                                                         <  1:1155478/150‑1 (MQ=255)

                                                 ttGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGGCCGGTGGGCGCAGTCCGGTGGCCGTCCTCGACGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCCGCCTGGCCGACGAGCGCTGGGTGCCGGAg                                                                                         <  2:6652430/150‑1 (MQ=255)

                                                 ttGCGCTCGGCGCGGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCGTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAg                                                                                         <  2:2238259/150‑1 (MQ=255)

                                                  tGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGt                                                                                        <  2:3846878/150‑1 (MQ=255)

                                                  tGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGt                                                                                        <  1:7146120/150‑1 (MQ=255)

                                                   gcgcTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTc                                                                                       >  1:2924759/1‑150 (MQ=255)

                                                      cTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCa                                                                                    >  1:1057158/1‑150 (MQ=255)

                                                       tCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCAt                                                                                   >  2:10404347/1‑150 (MQ=255)

                                                          gcgcTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGcc                                                                                >  1:4684780/1‑150 (MQ=255)

                                                           cgcgGGCCGAGCAGGGCCACGCGCTGCTGGTGGTGTCCGGGGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTGGAGCGAGGACCCGCTGGACTGGTCGCGGATCACCCTCAGCCAGGCCGACGCGCGCTGGGTGCCGGAGTCGCATGCCg                                                                               <  2:2115864/150‑1 (MQ=255)

                                                           cgcTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCg                                                                               >  1:5841422/1‑150 (MQ=255)

                                                            gcTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGa                                                                              <  2:4474987/150‑1 (MQ=255)

                                                            gcTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCGTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGa                                                                              <  2:9626264/150‑1 (MQ=255)

                                                                gCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGc                                                                          <  2:1763249/150‑1 (MQ=255)

                                                                 ccGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCa                                                                         <  2:5383354/150‑1 (MQ=255)

                                                                  cGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCaa                                                                        <  1:7711200/150‑1 (MQ=255)

                                                                    aGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACg                                                                      >  1:6030742/1‑150 (MQ=255)

                                                                     gCAGGCCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGc                                                                     >  1:9070481/1‑150 (MQ=255)

                                                                         ggCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGCTCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGc                                                                 >  1:8918564/1‑150 (MQ=255)

                                                                             aGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTgg                                                             <  2:4877604/150‑1 (MQ=255)

                                                                               gcgcTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGtt                                                           <  2:8295350/150‑1 (MQ=255)

                                                                               gcgcTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGtt                                                           <  2:7036675/150‑1 (MQ=255)

                                                                                cgcTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTc                                                          <  1:10007986/150‑1 (MQ=255)

                                                                                 gctgctGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTcg                                                         <  1:844763/150‑1 (MQ=255)

                                                                                   tgctgGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTcgcc                                                       <  1:3909805/150‑1 (MQ=255)

                                                                                     cgggtggtGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGTCTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTcgccgc                                                     <  2:4033981/148‑1 (MQ=255)

                                                                                      tggtggTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGACGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCgccgcc                                                    <  1:8941797/150‑1 (MQ=255)

                                                                                       ggtggtGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCa                                                   >  1:8997283/1‑150 (MQ=255)

                                                                                       ggtggtGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCa                                                   >  1:6653913/1‑150 (MQ=255)

                                                                                        gtggggTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCAc                                                  <  1:7790193/150‑1 (MQ=255)

                                                                                           gtgtCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTg                                               >  1:9270205/1‑150 (MQ=255)

                                                                                            tgtCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGc                                              <  1:6145729/150‑1 (MQ=255)

                                                                                              tCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTc                                            >  2:8798724/1‑150 (MQ=255)

                                                                                                cGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCg                                          >  2:3778871/1‑150 (MQ=255)

                                                                                                cGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCg                                          <  1:2463714/150‑1 (MQ=255)

                                                                                                 ggTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGt                                         >  1:9666472/1‑150 (MQ=255)

                                                                                                  gTGGGCGCAGTCCGGTGGCCGTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGGTCGCCGCCACCTGCTCCGTg                                        <  2:2287919/150‑1 (MQ=255)

                                                                                                    gggCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGc                                      <  2:9805010/150‑1 (MQ=255)

                                                                                                    gggCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGc                                      >  1:10176170/1‑150 (MQ=255)

                                                                                                     ggCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCATCTGCTCCGTGGCg                                     >  2:1269209/1‑150 (MQ=255)

                                                                                                     ggCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCg                                     >  1:1811952/1‑150 (MQ=255)

                                                                                                      gcgcAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGa                                    <  1:7871105/150‑1 (MQ=255)

                                                                                                       cgcAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAg                                   <  2:9464342/150‑1 (MQ=255)

                                                                                                        gcAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAgg                                  >  1:8148714/1‑150 (MQ=255)

                                                                                                            tCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCTACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAggcggc                              >  2:7309803/1‑150 (MQ=255)

                                                                                                            tCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAggcggc                              >  2:8539648/1‑150 (MQ=255)

                                                                                                            gccGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGCCGAGCGCGGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAggcggc                              <  2:9362673/149‑1 (MQ=255)

                                                                                                             ccGGGGGCCGTCCTCGACGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAGgcggcg                             <  2:5703185/150‑1 (MQ=255)

                                                                                                                gTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAGGCGGCGAAg                          >  1:3262591/1‑150 (MQ=255)

                                                                                                                 tGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAGGCGGCGAAgg                         >  1:1247260/1‑150 (MQ=255)

                                                                                                                 tGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAGGCGGCGAAgg                         <  1:5929473/150‑1 (MQ=255)

                                                                                                                    ccgtCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAGGCGGCGAAGgcgc                      <  2:2646070/147‑1 (MQ=255)

                                                                                                                     cTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAGGCGGCGAAGgcgcg                     >  2:6320798/1‑150 (MQ=255)

                                                                                                                      ttCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAGGCGGCGAAGgcgcgc                    >  1:1083456/1‑150 (MQ=255)

                                                                                                                         cTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAGGCGGCGAAGGCGCGCTTc                 >  1:6096947/1‑150 (MQ=255)

                                                                                                                         cTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAGGCGGCGAAGGCGCGCTTc                 >  2:10262313/1‑150 (MQ=255)

                                                                                                                          tCGACGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAGGCGGCGAAGGCGCGCTTCa                <  2:5403018/150‑1 (MQ=255)

                                                                                                                           cGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAGGCGGCGAAGGCGCGCTTCAt               >  1:2213553/1‑150 (MQ=255)

                                                                                                                            gAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAGGCGGCGAAGGCGCGCTTCATc              >  2:4583776/1‑150 (MQ=255)

                                                                                                                              aGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCCGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAGGCGGCGAAGGCGCGCTTCATCgg            <  2:6503860/150‑1 (MQ=255)

                                                                                                                               gCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAGGCGGCGAAGGCGCGCTTCATCGGc           >  2:6173403/1‑150 (MQ=255)

                                                                                                                                ccTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAGGCGGCGAAGGCGCGCTTCATCGGcc          <  2:10715896/150‑1 (MQ=255)

                                                                                                                                ccTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGGTCGCCGCCACCTGCTCCGTGGCGAGGCGGCGAAGGCGCGCTTCATCGGcc          <  1:9520974/150‑1 (MQ=255)

                                                                                                                                 cTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAGGCGGCGAAGGCGCGCTTCATCGGCct         <  2:2870684/150‑1 (MQ=255)

                                                                                                                                 cTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAGGCGGCGAAGGCGCGCTTCATCGGCct         >  2:9566038/1‑150 (MQ=255)

                                                                                                                                 cTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAGGCGGCGAAGGCGCGCTTCATCGGCct         <  2:5583357/150‑1 (MQ=255)

                                                                                                                                  ttGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAGGCGGCGAAGGCGCGCTTCATCGGCctc        <  2:8905666/150‑1 (MQ=255)

                                                                                                                                  ttGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAGGCGGCGAAGGCGCGCTTCATCGGCctc        <  1:8554552/150‑1 (MQ=255)

                                                                                                                                       cGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAGGCGGCGAAGGCGCGCTTCATCGGCCTCTACCa   <  1:2113454/150‑1 (MQ=255)

                                                                                                                                        gaggagCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAGGCGGCGAAGGCGCGCTTCATCGGCCTCTACCAg  >  1:1591826/1‑150 (MQ=255)

                                                                                                                                        gaggagCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAGGCGGCGAAGGCGCGCTTCATCGGCCTCTACCAg  >  1:5415250/1‑150 (MQ=255)

CAGCGCCGCCGAGCAGGCCCGCGGCCTGGCCGCCGAGGTCGCCGGCCGGTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGATCAGGTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAGGCGGCGAAGGCGCGCTTCATCGGCCTCTACCAG  >  NC_008463/2001668‑2001953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`