Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,149,425 T→C Q549Q (CAA→CAG)  PA14_RS09965 ← M14 family metallopeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,149,4250TC99.8% 1697.7 / NA 434Q549Q (CAA→CAG) PA14_RS09965M14 family metallopeptidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (222/211);  minor base A (0/1);  total (222/212)
Fisher's exact test for biased strand distribution p-value = 4.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 434 total aligned reads displayed.

CGCAGGCGTTTGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTTTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGCCCGGAATCCGGTT  >  NC_008463/2149279‑2149571
                                                                                                                                                  |                                                                                                                                                  
cgcAGGCGTTTGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGc                                                                                                                                                 <  1:4779672/150‑1 (MQ=255)
 gcAGGCGTTTGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCg                                                                                                                                                <  1:3294607/150‑1 (MQ=255)
  cAGGCGTTTGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCgg                                                                                                                                               >  1:4235159/1‑150 (MQ=255)
   aGGCGTTTGTCGCAGGCGGACTTCAGCGCCGGGTCTGCCGCCAGCATCTGGCGCGCCATCGGCTCCAGCACGTAGTCCGCCCGCTCCTCGGCGGCCACCAGGGTGGAAATGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGt                                                                                                                                              <  2:6571274/150‑1 (MQ=255)
    ggCGTTTGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGtt                                                                                                                                             <  1:4664986/150‑1 (MQ=255)
      cGTTTGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTgc                                                                                                                                           >  1:2286063/1‑150 (MQ=255)
      cGTTGGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTAGTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTgc                                                                                                                                           <  2:5621838/150‑1 (MQ=255)
        tttGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTgcag                                                                                                                                         <  1:6303645/150‑1 (MQ=255)
        tttGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTgcag                                                                                                                                         <  1:4078406/150‑1 (MQ=255)
         ttGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTgcagc                                                                                                                                        >  1:951700/1‑150 (MQ=255)
          tGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTgcagca                                                                                                                                       <  1:9645936/150‑1 (MQ=255)
              gAAGGCGGACTTCAGCGCCGGGTCGTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTc                                                                                                                                   <  1:118765/150‑1 (MQ=255)
               aaGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCg                                                                                                                                  <  1:7868655/150‑1 (MQ=255)
               aaGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCg                                                                                                                                  >  1:128031/1‑150 (MQ=255)
                aGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGa                                                                                                                                 >  1:5147245/1‑150 (MQ=255)
                     gACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGcc                                                                                                                            >  2:8234097/1‑150 (MQ=255)
                     gACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGcc                                                                                                                            >  1:3947635/1‑150 (MQ=255)
                      aCTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCa                                                                                                                           <  1:9847966/150‑1 (MQ=255)
                        ttCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAgg                                                                                                                         >  2:6756753/1‑150 (MQ=255)
                        ttCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAgg                                                                                                                         >  1:8858861/1‑150 (MQ=255)
                        ttCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAgg                                                                                                                         >  1:10714796/1‑150 (MQ=255)
                            gcgcCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGc                                                                                                                     >  1:10409092/1‑150 (MQ=255)
                            gcgcCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGc                                                                                                                     <  1:6022926/150‑1 (MQ=255)
                              gcCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGccc                                                                                                                   >  2:35177/1‑150 (MQ=255)
                              gcCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGccc                                                                                                                   <  1:7198915/150‑1 (MQ=255)
                                cGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAg                                                                                                                 <  1:1779504/150‑1 (MQ=255)
                                  ggTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCg                                                                                                               >  1:8684695/1‑150 (MQ=255)
                                     cTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCt                                                                                                            >  1:5500355/1‑150 (MQ=255)
                                        ccGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTggg                                                                                                         <  1:1726672/150‑1 (MQ=255)
                                           ccAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGACGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCgg                                                                                                      <  2:916043/150‑1 (MQ=255)
                                           ccAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCgg                                                                                                      <  2:2023762/150‑1 (MQ=255)
                                           ccAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCgg                                                                                                      >  1:4454774/1‑150 (MQ=255)
                                             aGCATCTTGCGCGCCATCGGCTCCATCACGTCGTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGgtg                                                                                                    <  2:9783127/150‑1 (MQ=255)
                                             aGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGgtg                                                                                                    >  1:7123411/1‑150 (MQ=255)
                                             aGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGgtg                                                                                                    >  2:3295578/1‑150 (MQ=255)
                                               cATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGtc                                                                                                  <  2:2985540/150‑1 (MQ=255)
                                                  cTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGtcgat                                                                                               <  1:6418173/150‑1 (MQ=255)
                                                  cTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGtcgat                                                                                               <  2:5681626/150‑1 (MQ=255)
                                                     gcgcgcCATCGGCTCCATCACGTATTCCTCCGGCGCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGtcgatcga                                                                                            <  2:4685045/150‑1 (MQ=255)
                                                      cgcgcCATCGGCTCCATCACGTAGTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGAc                                                                                           <  2:10553051/150‑1 (MQ=255)
                                                         gcCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCTGTGTCGATCGACAGc                                                                                        >  1:8223157/1‑150 (MQ=255)
                                                         gcCATCGGCTCCATCACGTAGTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGc                                                                                        <  2:6218143/150‑1 (MQ=255)
                                                           cATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGa                                                                                      <  1:9089164/150‑1 (MQ=255)
                                                            aTCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGGTCGACAGCGAg                                                                                     >  2:4971139/1‑150 (MQ=255)
                                                             tCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGACGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAgc                                                                                    >  1:6103843/1‑150 (MQ=255)
                                                             tCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAgc                                                                                    >  2:7826131/1‑150 (MQ=255)
                                                                 cTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAgccggc                                                                                >  1:7563802/1‑150 (MQ=255)
                                                                   ccATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAgccggccg                                                                              >  2:282637/1‑150 (MQ=255)
                                                                   ccATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAgccggccg                                                                              >  2:10727222/1‑150 (MQ=255)
                                                                    cATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGccggccgg                                                                             <  2:2510130/150‑1 (MQ=255)
                                                                    cATCACGTATTCCTCCGGCTCCACGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCCGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGccggccgg                                                                             <  2:2102111/150‑1 (MQ=255)
                                                                     aacACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCggg                                                                            <  1:10288503/148‑1 (MQ=255)
                                                                      tCACGTCTTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAAGTGAAGAAGCCCCAGGACCAGAACGAATCCGGACGCTGCGGTTGCAGCAGGTCGAGGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGa                                                                           <  2:6401282/150‑1 (MQ=255)
                                                                       cACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGaa                                                                          >  1:8490583/1‑150 (MQ=255)
                                                                         cGTATTCCTCCGGCTCCTCGGCGGCCCCCCGGGTGGAATTGAAGAAGCCCCAGGACCAGACCGAATCCGGACTCTGCGGTTGCAGCAGGTCGAGGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAgg                                                                        >  2:1231378/1‑150 (MQ=255)
                                                                          gTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGTAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGt                                                                       >  1:9322736/1‑150 (MQ=255)
                                                                            agtcctccGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCt                                                                     <  2:145217/148‑1 (MQ=255)
                                                                                 tccGGCTCCTCGGCGGCCACCAGGGTGGAAATGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAg                                                                <  2:5441015/150‑1 (MQ=255)
                                                                                  ccGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAgc                                                               <  2:9668555/150‑1 (MQ=255)
                                                                                  ccGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAgc                                                               >  1:357730/1‑150 (MQ=255)
                                                                                   cGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAgcg                                                              <  2:8469712/150‑1 (MQ=255)
                                                                                      ctcctcGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGACGGTCTGCAAGCGCTg                                                           >  2:7473102/1‑150 (MQ=255)
                                                                                      ctcctcGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTg                                                           >  2:4292955/1‑150 (MQ=255)
                                                                                        cctcGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGa                                                         >  2:2328211/1‑150 (MQ=255)
                                                                                        cctcGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGa                                                         >  2:3721436/1‑150 (MQ=255)
                                                                                            ggcggcCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACgg                                                     >  1:8996454/1‑150 (MQ=255)
                                                                                             gcggcCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGc                                                    <  2:5147245/150‑1 (MQ=255)
                                                                                             gcggcCACCAGGGGGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGc                                                    >  1:7893571/1‑150 (MQ=255)
                                                                                               ggcCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCtt                                                  >  2:8675938/1‑150 (MQ=255)
                                                                                                gcCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTc                                                 >  1:2980895/1‑150 (MQ=255)
                                                                                                 ccaccaGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCg                                                >  1:2132134/1‑150 (MQ=255)
                                                                                                  caccaGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCgg                                               >  2:9420961/1‑150 (MQ=255)
                                                                                                   accaGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGc                                              >  1:9960412/1‑150 (MQ=255)
                                                                                                    ccaGGGTGGAATTGAAGAAGCCCCTGGACCAGAACTAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCg                                             <  2:3105012/150‑1 (MQ=255)
                                                                                                      aGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTg                                           >  1:194421/1‑150 (MQ=255)
                                                                                                      aGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTg                                           >  2:1951003/1‑150 (MQ=255)
                                                                                                          tGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGc                                       >  1:6771717/1‑150 (MQ=255)
                                                                                                          tGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGc                                       >  2:7532560/1‑150 (MQ=255)
                                                                                                           ggAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCt                                      >  2:7062905/1‑150 (MQ=255)
                                                                                                           ggAATTGAAGAAGCCCCAGGACCAGAACGAAGCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCt                                      <  1:5796303/150‑1 (MQ=255)
                                                                                                             aaTTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCCGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGa                                    >  1:575242/1‑150 (MQ=255)
                                                                                                               ttGAATAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCGGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGAcc                                  >  1:10552516/1‑150 (MQ=255)
                                                                                                                tGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACca                                 <  1:2879102/150‑1 (MQ=255)
                                                                                                                     cagCCCCAGGACCAGAACGACTCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAgg                            <  2:949602/149‑1 (MQ=255)
                                                                                                                     aagCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAgg                            >  2:241055/1‑150 (MQ=255)
                                                                                                                          ccAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGAcc                       >  2:7954045/1‑150 (MQ=255)
                                                                                                                           cAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCt                      <  2:9884793/150‑1 (MQ=255)
                                                                                                                            aGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCtt                     >  2:802940/1‑150 (MQ=255)
                                                                                                                            aGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCtt                     <  1:8328036/150‑1 (MQ=255)
                                                                                                                            aGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCtt                     <  2:10111431/150‑1 (MQ=255)
                                                                                                                             ggACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGACGGTCTGCAAGCGCTGGAACGGCTGCGGCGTGCCGCTGACCAGCAGGCGACCTTc                    >  2:3947881/1‑150 (MQ=255)
                                                                                                                              gACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCg                   <  1:3565151/150‑1 (MQ=255)
                                                                                                                               aCCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGCCGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGt                  <  2:7158462/150‑1 (MQ=255)
                                                                                                                               aCCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGCGCCGGCCGGGAAGGTCTGCAAGCGCTGGACCGGCTTCGGCGTGCCGCTGACCCGCAGGCGACCTTCGt                  >  2:1821565/1‑150 (MQ=255)
                                                                                                                                ccAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTa                 >  2:10265822/1‑150 (MQ=255)
                                                                                                                                   gAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGcc              >  1:10082411/1‑150 (MQ=255)
                                                                                                                                     aCGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGCCCg            <  1:1468103/150‑1 (MQ=255)
                                                                                                                                           ccGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGCCCGGAATcc      >  2:2692608/1‑150 (MQ=255)
                                                                                                                                            cGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGCCCGGATTCCg     <  1:894504/150‑1 (MQ=255)
                                                                                                                                             ggACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGCCCGGAATCCgg    >  1:7411444/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CGCAGGCGTTTGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTTTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGCCCGGAATCCGGTT  >  NC_008463/2149279‑2149571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑