Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,318,606 A→G E190E (GAA→GAG)  sbrI → ECF family RNA polymerase sigma factor SbrI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,318,6060AG100.0% 1963.0 / NA 509E190E (GAA→GAG) sbrIECF family RNA polymerase sigma factor SbrI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (250/259);  total (250/259)

Only 100 of 517 total aligned reads displayed.

GCCCGAGGAACAGCGCGAGGTATTCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAAGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCTCGCCGCCGCGCGACGCCAGGCGGCGCAT  >  NC_008463/2318459‑2318752
                                                                                                                                                   |                                                                                                                                                  
gCCCGAGGACCAGCGCGAGGTATTCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAgga                                                                                                                                                  >  1:10727116/1‑150 (MQ=255)
gCCCGAGGAACAGCGCGAGGTATTCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGACGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAgga                                                                                                                                                  >  2:4966655/1‑150 (MQ=255)
     aGGAACAGCGCGAGGTATTCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTgg                                                                                                                                             >  2:6971959/1‑150 (MQ=255)
     aGGAACAGCGCGAGGTATTCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTgg                                                                                                                                             >  1:9246288/1‑150 (MQ=255)
      ggAACAGCGCGAGGTATTCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGc                                                                                                                                            >  1:1433637/1‑150 (MQ=255)
      ggAACAGCGCGAGGTATTCCTGATGCGCGCCCACCGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGc                                                                                                                                            <  1:9090158/150‑1 (MQ=255)
        aaCAGCGCGAGGTATTCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCgg                                                                                                                                          >  2:1385755/1‑150 (MQ=255)
        aaCAGCGCGAGGTATTCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCgg                                                                                                                                          >  1:990775/1‑150 (MQ=255)
         aCAGCGCGAGGTATTCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGt                                                                                                                                         >  2:5703198/1‑150 (MQ=255)
         aCAGCGCGAGGTATTCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGt                                                                                                                                         <  1:4305741/150‑1 (MQ=255)
          cAGCGCGAGGTATTCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTa                                                                                                                                        <  1:3569951/150‑1 (MQ=255)
           aGCGCGAGGTATTCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTAt                                                                                                                                       >  1:10382659/1‑150 (MQ=255)
            gcgcGAGGTATTCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATg                                                                                                                                      >  2:5799453/1‑150 (MQ=255)
               cgAGGTATTCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTCTGAAc                                                                                                                                   >  2:10222858/1‑150 (MQ=255)
               cgAGGTATTCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAAc                                                                                                                                   <  1:10286360/150‑1 (MQ=255)
                    tATTCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCcaca                                                                                                                              <  2:2147297/150‑1 (MQ=255)
                       tCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGaa                                                                                                                           <  2:431814/150‑1 (MQ=255)
                       tCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGaa                                                                                                                           >  2:8508821/1‑150 (MQ=255)
                        ccTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAg                                                                                                                          >  2:246636/1‑150 (MQ=255)
                          tGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCa                                                                                                                        >  2:3788351/1‑150 (MQ=255)
                           gATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTGCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCAt                                                                                                                       >  1:4630312/1‑150 (MQ=255)
                           gATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCAt                                                                                                                       <  2:4708451/150‑1 (MQ=255)
                           gATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCAt                                                                                                                       <  2:3980516/150‑1 (MQ=255)
                            aTGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCGACGCGGTGCAGAAGAATCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGTAACCACAgtatcagt                                                                                                                      >  1:159415/1‑143 (MQ=255)
                            aTGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGACGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCAtt                                                                                                                      <  2:1681755/150‑1 (MQ=255)
                             tGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTc                                                                                                                     <  1:7622129/150‑1 (MQ=255)
                             tGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTc                                                                                                                     <  1:73650/150‑1 (MQ=255)
                              gcgcgcCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTcc                                                                                                                    <  1:6649791/150‑1 (MQ=255)
                               cgcgcCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCcg                                                                                                                   <  2:6054792/150‑1 (MQ=255)
                                gcgcCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCcgc                                                                                                                  >  2:2270534/1‑150 (MQ=255)
                                       cGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTc                                                                                                           <  1:6983807/150‑1 (MQ=255)
                                         gagaACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGc                                                                                                         <  2:7588886/150‑1 (MQ=255)
                                          agaACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGcc                                                                                                        <  2:8895785/150‑1 (MQ=255)
                                             actggaactggCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGaa                                                                                                     <  2:1860276/150‑1 (MQ=255)
                                              ctggaactggCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAag                                                                                                    <  1:8494498/150‑1 (MQ=255)
                                               tggaactggCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAaga                                                                                                   >  2:8735273/1‑150 (MQ=255)
                                               tggaactggCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAaga                                                                                                   >  2:3503900/1‑150 (MQ=255)
                                                    ctggCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGGGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCGGAAGGGCAgg                                                                                              >  2:9704853/1‑149 (MQ=255)
                                                     tggCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGAt                                                                                             >  2:9146400/1‑150 (MQ=255)
                                                     tggCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGAt                                                                                             <  1:2166923/150‑1 (MQ=255)
                                                      ggCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATg                                                                                            <  2:9456549/150‑1 (MQ=255)
                                                      ggCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGGATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATg                                                                                            >  1:3904941/1‑150 (MQ=255)
                                                           agaTCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCGGgc                                                                                       >  2:10439796/1‑150 (MQ=255)
                                                             aTCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCGTTCCGCGAGCGTCGCCGAAGAGCAGATGCTGgcgc                                                                                     >  1:8153656/1‑150 (MQ=255)
                                                              tCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCa                                                                                    <  2:3643024/150‑1 (MQ=255)
                                                              tCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCa                                                                                    <  2:4442911/150‑1 (MQ=255)
                                                              tCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCa                                                                                    <  1:6429624/150‑1 (MQ=255)
                                                              tCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCAGTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCa                                                                                    <  2:2682170/150‑1 (MQ=255)
                                                              tCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGGATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGgcgcc                                                                                    >  1:5591268/1‑149 (MQ=255)
                                                                gCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCAtt                                                                                  <  2:2899778/150‑1 (MQ=255)
                                                                  cACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTc                                                                                <  1:4831420/150‑1 (MQ=255)
                                                                    cGCTCACCCCGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCcg                                                                              <  2:8025829/150‑1 (MQ=255)
                                                                      cTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCcgcg                                                                            >  2:9246785/1‑150 (MQ=255)
                                                                       tCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCgcgc                                                                           >  1:9595075/1‑150 (MQ=255)
                                                                       tCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCgcgc                                                                           <  2:10113073/150‑1 (MQ=255)
                                                                        cACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGcc                                                                          <  1:4101058/150‑1 (MQ=255)
                                                                        cACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGcc                                                                          <  2:5573214/150‑1 (MQ=255)
                                                                         aCCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGccc                                                                         <  1:1689363/150‑1 (MQ=255)
                                                                           ccAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCAc                                                                       >  1:8002130/1‑150 (MQ=255)
                                                                            cAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCAcg                                                                      >  2:5309537/1‑150 (MQ=255)
                                                                            cAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCAcg                                                                      <  1:2726443/150‑1 (MQ=255)
                                                                              gTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCAcgcg                                                                    >  1:6214553/1‑150 (MQ=255)
                                                                               tCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACgcgc                                                                   >  2:6492113/1‑150 (MQ=255)
                                                                               tCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACgcgc                                                                   <  1:1997907/150‑1 (MQ=255)
                                                                                  ccgcTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGcgccgc                                                                <  2:3123428/150‑1 (MQ=255)
                                                                                     cTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCgcagc                                                             >  2:1098930/1‑150 (MQ=255)
                                                                                     cTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCAGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTACGCGCCCACGCGCCgcagc                                                             >  1:3727867/1‑150 (MQ=255)
                                                                                       ggAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAATCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGcagcag                                                           >  1:7145886/1‑150 (MQ=255)
                                                                                        gagaCGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCagcagc                                                          <  2:590241/150‑1 (MQ=255)
                                                                                         agaCGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAgcc                                                         <  1:9717211/150‑1 (MQ=255)
                                                                                         agaCGGTGAAGAGCCGTGTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCGGGCCGTGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAgcc                                                         >  1:2536572/1‑150 (MQ=255)
                                                                                           aCGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAgccgg                                                       <  1:97893/150‑1 (MQ=255)
                                                                                           aCGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTAGGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAgccgg                                                       <  2:8456197/150‑1 (MQ=255)
                                                                                            cGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAgccggc                                                      <  1:3002349/150‑1 (MQ=255)
                                                                                             ggTGAAGAGCCGTTTCCGCTACGCGGTGCAGAATCTGCGCCGGCTGCTGGCCGCGGAGGTGGCGGTATGTACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAgccggcg                                                     >  1:3258893/1‑150 (MQ=255)
                                                                                              gTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAgccggcgc                                                    <  1:5860137/150‑1 (MQ=255)
                                                                                              gTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAgccggcgc                                                    >  2:6724585/1‑150 (MQ=255)
                                                                                                 aaGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGCAGCAGTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAgccggcgccgg                                                 <  1:401484/150‑1 (MQ=255)
                                                                                                 aaGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAgccggcgccgg                                                 >  2:6829254/1‑150 (MQ=255)
                                                                                                    agCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCcggcgccggcgc                                              >  2:4833335/1‑150 (MQ=255)
                                                                                                       cGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTgg                                           <  1:10038254/150‑1 (MQ=255)
                                                                                                         tttCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGAc                                         >  2:2970124/1‑150 (MQ=255)
                                                                                                           tCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCa                                       >  2:5232556/1‑150 (MQ=255)
                                                                                                            ccGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACACACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAg                                      >  1:3094034/1‑150 (MQ=255)
                                                                                                              gCTACGCGGTGCAGAAGCGGCGCCGGCTGCTGGCCGAGGTGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGc                                    <  1:5497361/150‑1 (MQ=255)
                                                                                                                 aCGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGAt                                 >  2:996046/1‑150 (MQ=255)
                                                                                                                  cgcgGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCCCAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATc                                <  1:10613325/150‑1 (MQ=255)
                                                                                                                    cgGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCt                              <  1:1162544/150‑1 (MQ=255)
                                                                                                                       tGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGACCCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCTcgc                           <  1:2270872/150‑1 (MQ=255)
                                                                                                                           gAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCTcgccgcc                       >  1:1911076/1‑150 (MQ=255)
                                                                                                                            aaGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCTcgccgccg                      >  2:1896925/1‑150 (MQ=255)
                                                                                                                                  cgcCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCTCGCCGCCGCGCGAc                <  2:460795/150‑1 (MQ=255)
                                                                                                                                  cgcCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCTCGCCGCCGCGCGAc                >  1:8903008/1‑150 (MQ=255)
                                                                                                                                    ccGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCTCGCCGCCGCGCGACGc              >  1:8405049/1‑150 (MQ=255)
                                                                                                                                     cGGCTGCTGGCCGAGGAGGTGGCGGTTTGAACCCACAGAAGCAGTCCGCGAGCGTCGCCGAAGAGCATAGGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCTCGCCGCCGCGCGACGcc             <  2:832344/150‑1 (MQ=255)
                                                                                                                                     cGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCTCGCCGCCGCGCGACGcc             >  2:10612729/1‑150 (MQ=255)
                                                                                                                                          gctgGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCTCGCCGCCGCGCGACGCCAggcg        >  1:8807490/1‑150 (MQ=255)
                                                                                                                                           ctgGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCTCGCCGCCGCGCGACGCCAggcgg       >  2:4064774/1‑150 (MQ=255)
                                                                                                                                              gCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCTCGCCGCCGCGCGACGCCAGGCGgcgc    >  1:514130/1‑150 (MQ=255)
                                                                                                                                               ccGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCTCGCCGCCGCGCGACGCCAGGCGGCGCa   >  2:7354411/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCCCGAGGAACAGCGCGAGGTATTCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAAGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCTCGCCGCCGCGCGACGCCAGGCGGCGCAT  >  NC_008463/2318459‑2318752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑