Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,762,024 A→G N125D (AAC→GAC)  PA14_RS12995 → lysophospholipid acyltransferase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,762,0240AG100.0% 1719.5 / NA 432N125D (AAC→GAC) PA14_RS12995lysophospholipid acyltransferase family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (219/213);  total (219/213)

Only 100 of 451 total aligned reads displayed.

GCAGCTACGTGATCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCAAGGGCACCCGCAACCTGAACAACAAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGTTGCCGCTGCTCTGC  >  NC_008463/2761878‑2762170
                                                                                                                                                  |                                                                                                                                                  
 cAGCTACGTGATCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGgacga                                                                                                                                                <  2:8865549/150‑3 (MQ=255)
   gCTACGTGATCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGgacgacg                                                                                                                                              >  1:1357717/1‑149 (MQ=255)
    cTACGTGATCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGgacgacga                                                                                                                                             <  2:4577734/150‑3 (MQ=255)
    cTACGTGATCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGgacgacga                                                                                                                                             >  2:6394552/1‑148 (MQ=255)
     tACGTGATCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAg                                                                                                                                            >  2:4273820/1‑150 (MQ=255)
       cGTGATCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGt                                                                                                                                          >  2:2080979/1‑150 (MQ=255)
        gTGATCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTc                                                                                                                                         <  1:8329805/150‑1 (MQ=255)
          gATCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCaa                                                                                                                                       <  2:3921419/150‑1 (MQ=255)
          gATCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCaa                                                                                                                                       <  1:10633115/150‑1 (MQ=255)
           aTCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGTGGGCACCCGCAACCTGGACGACGAGGTCAAg                                                                                                                                      <  2:9828676/150‑1 (MQ=255)
           aTCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAg                                                                                                                                      >  2:1364996/1‑150 (MQ=255)
           aTCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAg                                                                                                                                      <  1:4506086/150‑1 (MQ=255)
            tCAACAAGGTGTTCAACGCCGACCTAGTCGACCGCCAGCGAATCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGTACTTCGGCGATACGCTCATCATCTTCCCCGACGGCACCCGCAACCTGGACGACGAGGTCAAGc                                                                                                                                     >  2:9192305/1‑150 (MQ=255)
               acaaGGTGGTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGt                                                                                                                                  <  1:4892570/150‑1 (MQ=255)
                 aaGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTg                                                                                                                                <  2:9509519/150‑1 (MQ=255)
                    gtgtTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCa                                                                                                                             <  1:6774064/150‑1 (MQ=255)
                      gtTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCGGTTGCCAtt                                                                                                                           >  1:6284142/1‑150 (MQ=255)
                       ttCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTc                                                                                                                          <  2:8376582/150‑1 (MQ=255)
                       gtCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCCCCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTc                                                                                                                          <  1:3961399/149‑1 (MQ=255)
                        tCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCa                                                                                                                         <  1:1567459/150‑1 (MQ=255)
                                gTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGt                                                                                                                 >  2:5898138/1‑150 (MQ=255)
                                  gCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTAt                                                                                                               >  2:9636772/1‑150 (MQ=255)
                                   cTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATc                                                                                                              <  1:9408376/150‑1 (MQ=255)
                                   cTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATc                                                                                                              <  1:10267890/150‑1 (MQ=255)
                                     ggTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTa                                                                                                            >  2:7268883/1‑150 (MQ=255)
                                      gTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTac                                                                                                           >  2:3416062/1‑150 (MQ=255)
                                         gACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGAGTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTaccac                                                                                                        <  2:1154766/150‑1 (MQ=255)
                                            cGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTg                                                                                                     <  2:7550849/150‑1 (MQ=255)
                                             gCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGt                                                                                                    >  1:9120395/1‑150 (MQ=255)
                                             gCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGt                                                                                                    >  2:6280309/1‑150 (MQ=255)
                                             gCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGt                                                                                                    <  2:4778713/150‑1 (MQ=255)
                                              ccAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTc                                                                                                   >  1:6881260/1‑150 (MQ=255)
                                               cagcgcagcgATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTcc                                                                                                  >  1:8675961/1‑150 (MQ=255)
                                                    cagcgATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGc                                                                                             >  1:9533565/1‑150 (MQ=255)
                                                       cgATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCa                                                                                          <  2:10050684/150‑1 (MQ=255)
                                                         atgccaatgccaATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCAcc                                                                                        >  2:8488670/1‑150 (MQ=255)
                                                           gccaatgccaatCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCg                                                                                      <  1:4730743/150‑1 (MQ=255)
                                                            ccaatgccaatCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCgg                                                                                     <  1:3687960/150‑1 (MQ=255)
                                                            ccaatgccaatCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCgg                                                                                     >  2:3033032/1‑150 (MQ=255)
                                                             caatgccaatCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGa                                                                                    <  2:5673236/150‑1 (MQ=255)
                                                               atgccaatCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAgg                                                                                  >  2:7449350/1‑150 (MQ=255)
                                                               atgccaatCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAgg                                                                                  <  2:5225154/150‑1 (MQ=255)
                                                               atgccaatCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAgg                                                                                  >  1:8502708/1‑150 (MQ=255)
                                                               atgccaatCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAgg                                                                                  <  1:3362796/150‑1 (MQ=255)
                                                                tgccaatCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGt                                                                                 <  1:2953931/150‑1 (MQ=255)
                                                                    aatCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAg                                                                             <  1:733750/150‑1 (MQ=255)
                                                                    aatCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAg                                                                             >  2:9342733/1‑150 (MQ=255)
                                                                        cGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGGGTTTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCGGTTGCCATACAAGAGCGTTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTgg                                                                         <  1:8098336/150‑1 (MQ=255)
                                                                        cGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTgg                                                                         >  2:4898343/1‑150 (MQ=255)
                                                                         gCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGt                                                                        >  1:2074995/1‑150 (MQ=255)
                                                                          cTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTg                                                                       >  1:7539290/1‑150 (MQ=255)
                                                                           tCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGc                                                                      <  2:6976074/150‑1 (MQ=255)
                                                                           tCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGc                                                                      >  1:5085857/1‑150 (MQ=255)
                                                                           tCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGc                                                                      >  1:10540137/1‑150 (MQ=255)
                                                                           tCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGc                                                                      <  2:2313864/150‑1 (MQ=255)
                                                                           tCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGc                                                                      >  1:10381734/1‑150 (MQ=255)
                                                                              aaCCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCgg                                                                   >  1:4276084/1‑150 (MQ=255)
                                                                              aaCCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGGTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCgg                                                                   >  2:8151013/1‑150 (MQ=255)
                                                                                 cccTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTct                                                                <  1:10065558/150‑1 (MQ=255)
                                                                                 cccTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTct                                                                <  2:4022181/150‑1 (MQ=255)
                                                                                   cTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTctgg                                                              >  1:10754663/1‑150 (MQ=255)
                                                                                    tCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTctggc                                                             <  2:2282965/150‑1 (MQ=255)
                                                                                       aCCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTctggctgg                                                          >  2:9000235/1‑150 (MQ=255)
                                                                                       aCCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTctggctgg                                                          <  1:8434456/150‑1 (MQ=255)
                                                                                       aCCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTctggctgg                                                          <  1:9793825/150‑1 (MQ=255)
                                                                                        ccGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCtggctggc                                                         <  1:8832803/150‑1 (MQ=255)
                                                                                         cgcgCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGcc                                                        <  1:6352973/150‑1 (MQ=255)
                                                                                          gcgcCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCa                                                       >  1:6066824/1‑150 (MQ=255)
                                                                                            gcCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAAc                                                     <  2:5452453/150‑1 (MQ=255)
                                                                                              ccTGGACGGCGGCGACTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCAGTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCt                                                   <  2:7660489/150‑1 (MQ=255)
                                                                                               cTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTc                                                  >  1:57784/1‑150 (MQ=255)
                                                                                                tGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCa                                                 <  1:270228/150‑1 (MQ=255)
                                                                                                tGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCa                                                 >  1:2436547/1‑150 (MQ=255)
                                                                                                tGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCa                                                 >  1:6998357/1‑150 (MQ=255)
                                                                                                tGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCa                                                 >  2:2639348/1‑150 (MQ=255)
                                                                                                  gACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAAc                                               >  2:4267195/1‑150 (MQ=255)
                                                                                                   aCGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAAcc                                              <  2:1039886/150‑1 (MQ=255)
                                                                                                      gcggcgATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCggg                                           >  1:7195669/1‑150 (MQ=255)
                                                                                                          cgATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCAt                                       >  1:5507601/1‑150 (MQ=255)
                                                                                                            aTTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGc                                     >  1:897274/1‑150 (MQ=255)
                                                                                                               cGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCa                                  >  1:5369825/1‑150 (MQ=255)
                                                                                                                 cTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAg                                >  2:610016/1‑150 (MQ=255)
                                                                                                                 cTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAg                                <  2:3621324/150‑1 (MQ=255)
                                                                                                                 cTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAg                                <  1:4124342/150‑1 (MQ=255)
                                                                                                                         cTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCAt                        >  2:5625176/1‑150 (MQ=255)
                                                                                                                          ttCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGGTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCAtg                       <  2:5051410/150‑1 (MQ=255)
                                                                                                                           tCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCAtgc                      <  1:1767307/150‑1 (MQ=255)
                                                                                                                           tCCCCGAGGGCACCCGCAACCTCGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCAtgc                      <  2:10153919/150‑1 (MQ=255)
                                                                                                                            ccccGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCAtgct                     <  2:6807288/150‑1 (MQ=255)
                                                                                                                              ccgaGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCAtgctgc                   <  1:7874989/147‑1 (MQ=255)
                                                                                                                                gaGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCCTGCCCAAGGGCCGCTTGCTGCCg                 >  2:9106256/2‑150 (MQ=255)
                                                                                                                                  gggCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGtt               >  2:6602669/1‑150 (MQ=255)
                                                                                                                                  gggCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGtt               >  1:5942345/1‑150 (MQ=255)
                                                                                                                                      aCCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGTTGCCg           >  1:1202248/1‑150 (MQ=255)
                                                                                                                                       cccGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGTTGCCgc          <  2:218291/150‑1 (MQ=255)
                                                                                                                                          gCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGTTGCCgctgc       >  2:2941866/1‑150 (MQ=255)
                                                                                                                                           cAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGTTGCCgctgct      <  2:788095/150‑1 (MQ=255)
                                                                                                                                            aaCCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGTTGCCGCTGctc     >  1:9913604/1‑150 (MQ=255)
                                                                                                                                               cTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGTTGCCGCTGCTCTGc  <  1:9246151/150‑1 (MQ=255)
                                                                                                                                               cTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGTTGCCGCTGCTCTGc  <  2:2596053/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GCAGCTACGTGATCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCAAGGGCACCCGCAACCTGAACAACAAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGTTGCCGCTGCTCTGC  >  NC_008463/2761878‑2762170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑