BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	4,957,549	2 bp→AT	intergenic (‑371/‑300)	PA14_RS22700 ← / → PA14_RS22705	16S ribosomal RNA/exonuclease SbcCD subunit D C‑terminal domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	4,957,549	0	G	A	99.6%	471.2 / ‑4.7	234	intergenic (‑371/‑301)	PA14_RS22700/PA14_RS22705	16S ribosomal RNA/exonuclease SbcCD subunit D C‑terminal domain‑containing protein
Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (111/122); total (111/123)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01
*	NC_008463	4,957,550	0	C	T	99.6%	468.6 / NA	234	intergenic (‑372/‑300)	PA14_RS22700/PA14_RS22705	16S ribosomal RNA/exonuclease SbcCD subunit D C‑terminal domain‑containing protein
Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (111/122); total (111/123)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 234 total aligned reads displayed.

CGTTATTTTCGAAAATTTTCTTTTCTACTCAACCGCTTGCGCCGACCTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGATGAAGCTTCGTTTCGAGCCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCATTATAGGGAGATAGAAACT  >  NC_008463/4957418‑4957693

||

                                      gcgcCGACCTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTc                                                                                          >  1:1927833/1‑150 (MQ=9)

                                       cgcCGACCTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTcc                                                                                         <  2:8098273/150‑1 (MQ=12)

                                       ccccGACCTACAAACCCCCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACTACCTGCCGGAGCGCTTCACCACTcc                                                                                         <  1:5570649/148‑1 (MQ=11)

                                        gcCGACCTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCt                                                                                        >  2:803374/1‑150 (MQ=12)

                                         ccGACCTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTg                                                                                       >  1:841558/1‑150 (MQ=12)

                                           gACCTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTggg                                                                                     >  1:32981/1‑150 (MQ=12)

                                            aCCTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGga                                                                                    >  2:5321000/1‑150 (MQ=12)

                                            aCCTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGga                                                                                    >  2:5434498/1‑150 (MQ=12)

                                             ccTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGgag                                                                                   >  1:4020092/1‑150 (MQ=12)

                                             ccTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGgag                                                                                   <  1:10689185/150‑1 (MQ=12)

                                              cTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGgaga                                                                                  <  1:1084570/150‑1 (MQ=12)

                                                tCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGgagaga                                                                                >  2:2224173/1‑150 (MQ=12)

                                                tCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGgagaga                                                                                >  1:5996717/1‑150 (MQ=12)

                                                tCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGgagaga                                                                                >  1:4478492/1‑150 (MQ=12)

                                                  aaaCCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAAt                                                                              <  2:5783129/150‑1 (MQ=12)

                                                   aaCCATCATCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATc                                                                             <  2:7941271/150‑1 (MQ=255)

                                                      cATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGt                                                                          >  2:2360366/1‑150 (MQ=12)

                                                      cATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGt                                                                          <  1:6097351/150‑1 (MQ=12)

                                                       aTCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACAACTCCTGGGAGAGAATCCGTa                                                                         >  1:10143225/1‑150 (MQ=255)

                                                         cTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTaaa                                                                       <  2:476123/150‑1 (MQ=12)

                                                          ttCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAAt                                                                      >  1:7657951/1‑150 (MQ=12)

                                                           tCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATc                                                                     <  1:10228197/150‑1 (MQ=12)

                                                             cGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGAACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCt                                                                   <  2:4724280/150‑1 (MQ=255)

                                                              gATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCtt                                                                  >  2:1411476/1‑150 (MQ=12)

                                                                tCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGa                                                                >  2:3939889/1‑150 (MQ=12)

                                                                tCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGa                                                                <  2:3441908/150‑1 (MQ=12)

                                                                 cAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGAt                                                               <  2:369976/150‑1 (MQ=12)

                                                                  aGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGAtt                                                              <  2:3160151/150‑1 (MQ=12)

                                                                  aGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGAtt                                                              >  1:7310963/1‑150 (MQ=12)

                                                                  aGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGAtt                                                              <  1:10681075/150‑1 (MQ=12)

                                                                   gCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGAttt                                                             <  2:7130529/150‑1 (MQ=12)

                                                                   gCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGAttt                                                             >  2:4829697/1‑150 (MQ=12)

                                                                    cGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGAtttt                                                            <  1:1504083/150‑1 (MQ=12)

                                                                     gggAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTc                                                           <  1:5874413/150‑1 (MQ=12)

                                                                      ggAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCa                                                          >  1:671890/1‑150 (MQ=12)

                                                                       gAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCaa                                                         <  1:7992906/150‑1 (MQ=12)

                                                                         ggCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGACTCAGTAAATCCTTGATTTTCAAGc                                                       >  2:9684516/1‑150 (MQ=11)

                                                                         ggCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGc                                                       <  2:8185561/150‑1 (MQ=12)

                                                                         ggCGCATTCTACAGCGTTCAAAGTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGc                                                       <  2:10556520/150‑1 (MQ=255)

                                                                          gcgcATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCa                                                      <  1:3759803/150‑1 (MQ=12)

                                                                          gcgcATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCa                                                      >  2:2657532/1‑150 (MQ=12)

                                                                            gcATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCAtt                                                    >  1:5044511/1‑150 (MQ=12)

                                                                            gcATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCAtt                                                    <  1:1441583/150‑1 (MQ=12)

                                                                            gcATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCAtt                                                    <  1:6375024/150‑1 (MQ=12)

                                                                            gcATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCAtt                                                    <  2:5907949/150‑1 (MQ=12)

                                                                             cATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCAttt                                                   >  1:1896479/1‑150 (MQ=12)

                                                                              aTTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTa                                                  >  1:1254797/1‑150 (MQ=12)

                                                                                tCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCTAGCATTTAct                                                >  2:6076060/1‑150 (MQ=255)

                                                                                 cTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTActc                                               <  1:2395096/150‑1 (MQ=12)

                                                                                  tACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTActct                                              <  1:3424314/150‑1 (MQ=12)

                                                                                   aCAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCtt                                             >  2:6353061/1‑150 (MQ=12)

                                                                                   aCAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCtt                                             <  2:8115763/150‑1 (MQ=12)

                                                                                    cAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTtc                                            <  1:9670187/150‑1 (MQ=12)

                                                                                     aGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTtcg                                           >  2:3385973/1‑150 (MQ=12)

                                                                                      gCGTTCACATTCGCTGACAAGCACCTCGGAGACGCTTCGTATCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAAGCCTTGATTTTCAAGCATTTACTCTtcgt                                          <  2:10259616/150‑1 (MQ=11)

                                                                                       cGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTtcgtc                                         <  2:10523100/150‑1 (MQ=12)

                                                                                       cGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTtcgtc                                         <  2:7476397/150‑1 (MQ=12)

                                                                                       cGTTCAAATTCACTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTtcgtc                                         <  1:649572/150‑1 (MQ=255)

                                                                                         ttCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTtcgtcgt                                       <  2:4603211/150‑1 (MQ=12)

                                                                                         ttCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTtcgtcgt                                       >  2:4427471/1‑150 (MQ=12)

                                                                                          tCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGCGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTtcgtcgtc                                      >  2:8957159/1‑150 (MQ=255)

                                                                                          tCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTtcgtcgtc                                      <  1:100997/150‑1 (MQ=12)

                                                                                            aaaTTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGc                                    <  2:6700379/150‑1 (MQ=12)

                                                                                            aaaTTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGc                                    <  2:9843517/150‑1 (MQ=12)

                                                                                             aaTTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGcc                                   >  2:309148/1‑150 (MQ=12)

                                                                                             aaTTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGcc                                   <  1:3333571/150‑1 (MQ=12)

                                                                                              aTTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCg                                  <  2:1578170/150‑1 (MQ=12)

                                                                                                     gTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAgtgg                           <  2:9069391/150‑1 (MQ=12)

                                                                                                     gTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAgtgg                           >  1:3359266/1‑150 (MQ=12)

                                                                                                       cAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAgtggtg                         >  2:3779125/1‑150 (MQ=12)

                                                                                                       cAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAgtggtg                         >  1:9704920/1‑150 (MQ=12)

                                                                                                       cAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAgtggtg                         <  2:5395210/150‑1 (MQ=12)

                                                                                                         aGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTgcg                       >  1:2361751/1‑150 (MQ=12)

                                                                                                          gCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTgcgc                      >  2:1136076/1‑150 (MQ=12)

                                                                                                              cTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCATta                  >  1:8737232/1‑150 (MQ=12)

                                                                                                              cTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCATta                  <  1:5017745/150‑1 (MQ=12)

                                                                                                               tCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCATtat                 >  2:7604685/1‑150 (MQ=12)

                                                                                                                cggtGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCATtata                <  2:5116434/147‑1 (MQ=12)

                                                                                                                   tGAAGCTTCGTTTCGAATCCCTTTCTGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCATTATAggg             <  2:1189076/150‑1 (MQ=12)

                                                                                                                   tGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCATTATAggg             <  1:9405048/150‑1 (MQ=17)

                                                                                                                   tGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCATTATAggg             >  1:4408906/1‑150 (MQ=17)

                                                                                                                     aaGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCATTATAGGgag           >  2:8344004/1‑150 (MQ=17)

                                                                                                                      aGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCTGCGAACAACCTGCGGGAGGGCTTCACCACTCGTGGGAGAGAATCCGTAAATCGTTGATTTTCAAGCATTTTCTCTTCGTCGTCGCCGGGAGGGGTGCGCATTATAGGgaga          >  2:3651399/1‑150 (MQ=11)

                                                                                                                      aGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCATTATAGGgaga          >  1:2852597/1‑150 (MQ=17)

                                                                                                                      aGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCATTATAGGgaga          <  1:636476/150‑1 (MQ=17)

                                                                                                                       gCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCATTATAGGGAGAt         >  1:8970152/1‑150 (MQ=17)

                                                                                                                       gCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCATTATAGGGAGAt         <  2:1707823/150‑1 (MQ=17)

                                                                                                                       gCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCATTATAGGGAGAt         <  1:4583437/150‑1 (MQ=17)

                                                                                                                        cTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCATTATAGGGAGATa        <  1:9498451/150‑1 (MQ=17)

                                                                                                                        cTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCAGTATAGGGAGATa        >  1:9156664/1‑150 (MQ=12)

                                                                                                                          tCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCATTATAGGGAGATAGa      >  1:2204928/1‑150 (MQ=17)

                                                                                                                          tCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCATTATAGGGAGATAGa      >  2:4090457/1‑150 (MQ=17)

                                                                                                                           cGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCATTATAGGGAGATAGaa     <  1:6911872/150‑1 (MQ=17)

                                                                                                                           cGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCATTATAGGGAGATAGaa     <  2:8769347/150‑1 (MQ=17)

                                                                                                                           cGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCATTATAGGGAGATAGaa     <  2:9275418/150‑1 (MQ=17)

                                                                                                                           cGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCATTATAGGGAGATAGaa     <  1:9982607/150‑1 (MQ=17)

                                                                                                                            gTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCATTATAGGGAGATAGaaa    <  2:1663400/150‑1 (MQ=17)

                                                                                                                            gTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCATTATAGGGAGATAGaaa    <  2:7056794/150‑1 (MQ=17)

                                                                                                                             tttCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCATTATAGGGAGATAGAAAc   >  2:5677472/1‑150 (MQ=17)

                                                                                                                              ttCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCATTATAGGGAGATAGAAACt  >  2:695640/1‑150 (MQ=17)

||

CGTTATTTTCGAAAATTTTCTTTTCTACTCAACCGCTTGCGCCGACCTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGATGAAGCTTCGTTTCGAGCCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCATTATAGGGAGATAGAAACT  >  NC_008463/4957418‑4957693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`