Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 1,128,156 3 bp→GCG intergenic (+17/‑42) PA14_RS05280 → / → PA14_RS05285 DUF1302 domain‑containing protein/DUF1329 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084631,128,1560NG100.0% 1528.7 / NA 404intergenic (+17/‑44)PA14_RS05280/PA14_RS05285DUF1302 domain‑containing protein/DUF1329 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base N (0/0);  new base G (211/193);  total (211/193)
*NC_0084631,128,1570NC99.8% 1481.5 / ‑4.3 404intergenic (+18/‑43)PA14_RS05280/PA14_RS05285DUF1302 domain‑containing protein/DUF1329 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base N (0/0);  major base C (211/192);  minor base A (0/1);  total (211/193)
Fisher's exact test for biased strand distribution p-value = 4.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01
*NC_0084631,128,1580NG99.8% 1502.5 / NA 404intergenic (+19/‑42)PA14_RS05280/PA14_RS05285DUF1302 domain‑containing protein/DUF1329 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base N (0/0);  major base G (210/192);  minor base T (0/1);  total (211/193)
Fisher's exact test for biased strand distribution p-value = 4.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 404 total aligned reads displayed.

CCTGGGGGTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTNNNANCNGGNCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCGGC  >  NC_008463/1128010‑1128304
                                                                                                                                                  |||                                                                                                                                                  
ccTGGGGGTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTCCACCCTCTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGa                                                                                                                                                   >  2:7797424/1‑150 (MQ=255)
 cTGGGGGTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGaa                                                                                                                                                  <  1:5809366/150‑2 (MQ=255)
   gggggTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAAcc                                                                                                                                                <  2:6845554/150‑2 (MQ=255)
    ggggTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCg                                                                                                                                               <  1:3890516/150‑1 (MQ=255)
     gggTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCgg                                                                                                                                              <  1:4453486/150‑1 (MQ=255)
     gggTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACGTCTGATGGACGCCGGCCCGGTGCGAACCgg                                                                                                                                              >  1:2069319/1‑150 (MQ=255)
      ggTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCggg                                                                                                                                             <  2:2107600/150‑2 (MQ=255)
         ggACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCTCCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCCTGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCg                                                                                                                                          >  2:9370127/1‑150 (MQ=255)
               ggAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGc                                                                                                                                    >  2:2551099/1‑150 (MQ=255)
                gAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCa                                                                                                                                   >  2:3814771/1‑150 (MQ=255)
                gAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGAGCGCGACTTCGTCGCGCTCAGGTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGTACCGGGCCGGCGAGCa                                                                                                                                   >  1:2758964/1‑150 (MQ=255)
                  gTACCAGAACCCCTACACCGCGAGCCTCTCCTACACCAACTTCTGCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAgg                                                                                                                                 <  2:8476060/150‑1 (MQ=255)
                       aGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTa                                                                                                                            >  2:1297959/1‑150 (MQ=255)
                         aaCACCTACACCGCGAGCCTCTCATACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAAc                                                                                                                          <  1:346363/150‑1 (MQ=255)
                          acacCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGACCCGGGCCGGCGAGCAGGACTTAACg                                                                                                                         >  2:1049992/1‑150 (MQ=255)
                           cacCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGa                                                                                                                        >  2:10076755/1‑150 (MQ=255)
                           cacCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTACGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGa                                                                                                                        <  2:5457231/150‑1 (MQ=255)
                               tACACCGCGCGCCTCTCCTACACCACCTTCTTCGACGGAAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATTAACTTCTGATGTACGCCGGCCGGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGgac                                                                                                                    <  1:930555/150‑1 (MQ=255)
                               tACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACNGTGGACGAGCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGgac                                                                                                                    >  1:5670654/1‑150 (MQ=255)
                                acacCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACATCGTCGCGCTCAGCGTCGGCATGAACTTCTGATGTACGCCGGCCCCGTGCGACCCGGGCCGGCGAGCCCGACTTAAAGAGgacg                                                                                                                   >  1:2988566/1‑150 (MQ=255)
                                 cacCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGgacga                                                                                                                  >  2:4063722/1‑150 (MQ=255)
                                    cgcgAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGAcgacga                                                                                                               <  2:1435915/150‑1 (MQ=255)
                                     gcgAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAg                                                                                                              <  1:5447579/150‑1 (MQ=255)
                                      cgAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGc                                                                                                             >  2:1230909/1‑150 (MQ=255)
                                       gAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTAAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCg                                                                                                            >  2:6730376/1‑150 (MQ=255)
                                       gAGCCTCTCCTACACAAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCg                                                                                                            <  2:4428906/150‑1 (MQ=255)
                                        aGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCCGTGCGAACCGGGCCGGCGAGCAGGACTTAAAGAGGACGTCGAGCGa                                                                                                           >  1:10038791/1‑150 (MQ=255)
                                         gCCTCTCCTACACCCACTTCTACGACGGCAAGTACCCCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGaa                                                                                                          <  1:7230716/150‑1 (MQ=255)
                                         gCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGaa                                                                                                          >  1:6716371/1‑150 (MQ=255)
                                         gCCTCTCCTACACCAACTTCATCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGaa                                                                                                          <  1:7684363/150‑1 (MQ=255)
                                           ctctCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATg                                                                                                        <  1:490602/150‑1 (MQ=255)
                                           ctctCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATg                                                                                                        >  2:6635278/1‑150 (MQ=255)
                                           ctctCCTACACCAACTACTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATg                                                                                                        <  1:7492947/150‑1 (MQ=255)
                                            tctcCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGGCGCGCTCCGCTTCGGCATGAACTTCTGATGTAAGCCGGCCCGGTGCGAACCGGGCCGGCGAGCCGGACTTAACGAGGACGACGACCGAATgt                                                                                                       >  1:2350351/1‑149 (MQ=255)
                                              tcCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCAAGAACTTCTGATGTACGCCGGCCCGGTGCGACCCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGaaa                                                                                                     <  2:6436792/150‑1 (MQ=255)
                                               ccTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGCGCAGGACTTAACGAGGACGACGAGCGAATTaaaa                                                                                                    >  1:8242048/1‑150 (MQ=255)
                                               ccTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGaaaa                                                                                                    >  1:7983489/1‑150 (MQ=255)
                                                cTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAaac                                                                                                   <  2:9823760/150‑1 (MQ=255)
                                                cTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAaac                                                                                                   <  1:8652906/150‑1 (MQ=255)
                                                  acacCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGTACTTATGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAAGGAAaacca                                                                                                 >  1:7422495/1‑148 (MQ=255)
                                                     ccAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGa                                                                                              <  1:5352244/150‑1 (MQ=255)
                                                      cAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGaa                                                                                             <  2:7193572/150‑1 (MQ=255)
                                                        aCTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCCTGAACTTCTGATGTACGCCGGCCCGGTGCGACCCGGGCCGGCGAGCAGGACTTTACGAGGACGACGAGCGAATGAAAAAAACGAAGa                                                                                           >  1:2209317/1‑150 (MQ=255)
                                                         cttcttCGACGGCAAGTACCCCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGAt                                                                                          <  2:5347427/150‑1 (MQ=255)
                                                             ttcGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGAttctt                                                                                      <  1:1656786/150‑1 (MQ=255)
                                                                 aCGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGc                                                                                  <  2:5858492/150‑1 (MQ=255)
                                                                    gCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATa                                                                               >  1:6989711/1‑150 (MQ=255)
                                                                      aaGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACATAACGAGGACGACGAGCGCATGAAAAAAACGAATATTCTTCTGCATaac                                                                             >  2:2781363/1‑148 (MQ=255)
                                                                      aaGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCGGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATAcc                                                                             >  2:700524/1‑150 (MQ=255)
                                                                       aGTACACCACCGTGGACGATCGCGACATCGTCGCGCGCAGCCTCGGCCTGTACTTCTGTTGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGCCGACGAGCGACTGAAAACAACGAAGTTTCTTCTGCATACCg                                                                            >  1:4302433/1‑150 (MQ=255)
                                                                        gTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCgg                                                                           <  1:5535714/150‑1 (MQ=255)
                                                                        gTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCgg                                                                           >  2:8742040/1‑150 (MQ=255)
                                                                          acacCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGACCCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCgggg                                                                         <  1:135038/150‑3 (MQ=255)
                                                                          acacCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGTAAACAACGAAGATTCTTCTGCATACCGgtg                                                                         >  2:7442691/1‑150 (MQ=255)
                                                                          acacCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACCACGAAGATTCTTCTGCATACCGgtg                                                                         >  2:10748190/1‑150 (MQ=255)
                                                                           caccacCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGgtgt                                                                        <  2:8973656/150‑1 (MQ=255)
                                                                             ccaccGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTAc                                                                      <  1:9701091/150‑1 (MQ=255)
                                                                             ccaccGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCAAGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTAc                                                                      <  1:10576188/150‑1 (MQ=255)
                                                                                ccGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTgg                                                                   >  2:4176449/1‑150 (MQ=255)
                                                                                 cGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGc                                                                  >  2:6200603/1‑150 (MQ=255)
                                                                                 cGTGGACGATCGCGACTTCGTCGCGCTCAGCTTAGGCATGAACATATGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGAAGACGAGCGAATGAAAACAAAGAAGATTCTTCTGCAAACCGGTGTACAGGc                                                                  >  2:7234710/1‑150 (MQ=255)
                                                                                  gTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCAGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGcc                                                                 >  2:5135112/1‑150 (MQ=255)
                                                                                       cGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAg                                                            >  1:875707/1‑150 (MQ=255)
                                                                                        gATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGc                                                           >  2:3652088/1‑150 (MQ=255)
                                                                                         aTCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGcc                                                          <  1:9675499/150‑1 (MQ=255)
                                                                                         aTCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGcc                                                          >  1:708892/1‑150 (MQ=255)
                                                                                         aTCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGcc                                                          >  2:5503803/1‑150 (MQ=255)
                                                                                          tCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCct                                                         >  2:764293/1‑150 (MQ=255)
                                                                                            gcgACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCctgc                                                       <  1:10430294/150‑1 (MQ=255)
                                                                                               gcTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGTGGACGTCGTGCGAATGAAAACAACGACGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTgg                                                    >  1:2583836/2‑150 (MQ=255)
                                                                                               acgtcgtcgCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTgg                                                    <  2:7974077/147‑1 (MQ=255)
                                                                                                    gtcgCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGACTGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCAcc                                               >  2:8198778/1‑150 (MQ=255)
                                                                                                          cTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTg                                         >  2:9467383/1‑150 (MQ=255)
                                                                                                             aGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATg                                      >  2:3088640/1‑150 (MQ=255)
                                                                                                              gCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATgg                                     <  2:10604500/150‑1 (MQ=255)
                                                                                                                ttCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATggcg                                   >  1:10659048/1‑150 (MQ=255)
                                                                                                                 tCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCCGGTGATggcgg                                  <  2:2241350/150‑1 (MQ=255)
                                                                                                                 tCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATggcgg                                  >  2:1824763/1‑150 (MQ=255)
                                                                                                                 tCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATggcgg                                  >  1:9444416/1‑150 (MQ=255)
                                                                                                                 tCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATggcgg                                  <  1:593773/150‑1 (MQ=255)
                                                                                                                 tCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATggcgg                                  >  1:7995612/1‑150 (MQ=255)
                                                                                                                    gCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGcggcgg                               <  1:10689/150‑1 (MQ=255)
                                                                                                                      aTGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTa                             >  1:6874997/1‑150 (MQ=255)
                                                                                                                       tGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTAt                            <  2:3259617/150‑1 (MQ=255)
                                                                                                                         aaCTTCTGCTGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATcc                          <  1:6239781/150‑1 (MQ=255)
                                                                                                                          aCTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATccg                         >  2:1656493/1‑150 (MQ=255)
                                                                                                                             tCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATccgccg                      <  1:9855372/150‑1 (MQ=255)
                                                                                                                                gATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCcgacg                   <  1:5049411/150‑1 (MQ=255)
                                                                                                                                gATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCcgacg                   <  2:9679926/150‑1 (MQ=255)
                                                                                                                                    tACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAggc               >  2:9863600/1‑150 (MQ=255)
                                                                                                                                     aCGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGTCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAggcg              >  2:3406428/1‑150 (MQ=255)
                                                                                                                                     aCGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAggcg              >  2:8809767/1‑150 (MQ=255)
                                                                                                                                            cccGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGc       <  2:5704629/150‑1 (MQ=255)
                                                                                                                                            cccGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGc       >  2:2646617/1‑150 (MQ=255)
                                                                                                                                            cccGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGc       >  1:6535336/1‑150 (MQ=255)
                                                                                                                                             ccGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCt      >  1:3258151/1‑150 (MQ=255)
                                                                                                                                              cGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTc     >  1:8885994/1‑150 (MQ=255)
                                                                                                                                              cGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTc     >  1:8390172/1‑150 (MQ=255)
                                                                                                                                              cGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTc     <  1:676550/150‑1 (MQ=255)
                                                                                                                                                gTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCgg   >  1:10293105/1‑150 (MQ=255)
                                                                                                                                                  |||                                                                                                                                                  
CCTGGGGGTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTNNNANCNGGNCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCGGC  >  NC_008463/1128010‑1128304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑