BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	2,730,681	2 bp→CG	intergenic (+19/+95)	PA14_RS12860 → / ← ctpH	anti‑virulence regulator CigR family protein/methyl‑accepting chemotaxis protein CtpH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	2,730,681	0	G	C	100.0%	1525.1 / NA	386	intergenic (+19/+96)	PA14_RS12860/ctpH	anti‑virulence regulator CigR family protein/methyl‑accepting chemotaxis protein CtpH
Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (181/205); total (181/205)
*	NC_008463	2,730,682	0	C	G	99.7%	1491.9 / NA	386	intergenic (+20/+95)	PA14_RS12860/ctpH	anti‑virulence regulator CigR family protein/methyl‑accepting chemotaxis protein CtpH
Reads supporting (aligned to +/- strand): ref base C (0/0); major base G (181/204); minor base T (0/1); total (181/205)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 386 total aligned reads displayed.

GGCCGCCTGCTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGCAGGCCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGTTGCGCGGAGAGG  >  NC_008463/2730537‑2730822

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ggCCGCCTGCTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCAt                                                                                                                                          <  1:7506476/150‑1 (MQ=255)

ggCCGCCTGCTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCAt                                                                                                                                          <  1:2414115/150‑1 (MQ=255)

  ccgccTGCTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAg                                                                                                                                        <  1:1868436/150‑1 (MQ=255)

     ccTGCTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGgcg                                                                                                                                     >  2:6829786/1‑150 (MQ=255)

      cTGCTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGgcgc                                                                                                                                    >  1:1162326/1‑150 (MQ=255)

       tGCTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCt                                                                                                                                   <  1:6917519/150‑1 (MQ=255)

        gCTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTa                                                                                                                                  >  1:6979990/1‑150 (MQ=255)

          tAGGCCGGCTGCCGCACTACGACGGCTACGCGTGGATGCAGGCCGGCACCGACCTGCTGCGGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATc                                                                                                                                <  2:7468796/150‑1 (MQ=255)

          tAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCCTAGGCGCTATc                                                                                                                                >  2:1969073/1‑150 (MQ=255)

               cGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCa                                                                                                                           <  1:7934805/150‑1 (MQ=255)

                 gCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAgg                                                                                                                         >  1:2253732/1‑150 (MQ=255)

                  cTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGc                                                                                                                        <  1:9103615/150‑1 (MQ=255)

                    gCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTTGCGCCATAGGCGCTATCCGCCAGGccc                                                                                                                      <  1:4277946/150‑1 (MQ=255)

                    gCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCACTCGACTGAGCGGGACAGGGCGGGTATCGCCATAGGCGCTATCCGCCAGGccc                                                                                                                      >  2:10054034/1‑150 (MQ=255)

                      cGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCg                                                                                                                    <  1:713025/150‑1 (MQ=255)

                      cGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCg                                                                                                                    >  1:4053707/1‑150 (MQ=255)

                      cGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCGGCTGGGGGCGGGGGCGACGGGGCTGATCTACGAAGTGCTGGAAAACGCTCTCGACTGAGGGGGCCAGGGCGGGTAGCGCAATAGGCGCTATCCGCCAGGCCCCg                                                                                                                    <  1:5446244/150‑1 (MQ=255)

                       gCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGt                                                                                                                   <  2:3020120/150‑1 (MQ=255)

                        cACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGATGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGtt                                                                                                                  <  1:2786335/150‑1 (MQ=255)

                        cACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGtt                                                                                                                  <  1:2559558/150‑1 (MQ=255)

                         aCTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCAGAGCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGAGCTCTCCGCCAGGCCCCGTTg                                                                                                                 >  2:1786407/1‑150 (MQ=255)

                            acgacgGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAg                                                                                                              >  2:5100507/1‑150 (MQ=255)

                             cgacgGCTACGAGTGGATGCAGGCCGGCACCGCCCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTTTCCGCCAGGCCCCGTTGGAGt                                                                                                             <  2:4565430/150‑1 (MQ=255)

                             cgacgGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGt                                                                                                             <  2:841292/150‑1 (MQ=255)

                             cgacgGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGt                                                                                                             >  2:5416282/1‑150 (MQ=255)

                               acgGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTgg                                                                                                           >  1:3080856/1‑150 (MQ=255)

                                cgGCTACGAGTGGATGCAGGCCGTCACCTACCTGCTGCTGGTGGCGGTGGCGACCGGGATGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCTGGCCCCGTTGGAGTGGc                                                                                                          <  1:9432031/150‑1 (MQ=255)

                                cgGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGc                                                                                                          >  2:5021461/1‑150 (MQ=255)

                                cgGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGc                                                                                                          >  2:936936/1‑150 (MQ=255)

                                cgGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGc                                                                                                          <  1:10006812/150‑1 (MQ=255)

                                 ggCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCg                                                                                                         >  2:6111304/1‑150 (MQ=255)

                                 ggCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCg                                                                                                         <  2:8814021/150‑1 (MQ=255)

                                    tccGAGTGTATGCAGGCCGCCACCGACCTGCTGCTGGTGGCGGTGGCGCCCGGGCTGATCGACGCAGTGCTGCACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGATGGAGTGGCGGAt                                                                                                      <  1:1828349/148‑1 (MQ=255)

                                    tACGTGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGGGGCGACCGGGCTGATCTACGATGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATTGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGAt                                                                                                      <  1:8603969/150‑1 (MQ=255)

                                     aCGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCACTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATa                                                                                                     <  1:974824/150‑1 (MQ=255)

                                       gAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAAc                                                                                                   >  2:7037086/1‑150 (MQ=255)

                                         gTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCg                                                                                                 >  1:3935305/1‑150 (MQ=255)

                                           ggATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCgtg                                                                                               >  1:7370117/1‑150 (MQ=255)

                                            gATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCgtgt                                                                                              >  2:817137/1‑150 (MQ=255)

                                             aTGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGtt                                                                                             <  2:2721560/150‑1 (MQ=255)

                                             aTGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGtt                                                                                             <  1:2038234/150‑1 (MQ=255)

                                             aTGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGtt                                                                                             <  2:9117130/150‑1 (MQ=255)

                                             aTGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGtt                                                                                             >  2:655146/1‑150 (MQ=255)

                                               gCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTgc                                                                                           >  1:6073245/1‑150 (MQ=255)

                                                 aGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGTAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCGAGGCCCCGTTGGAGTGGCGGATAACCGTGTTgcgc                                                                                         >  1:9671243/1‑150 (MQ=255)

                                                     cGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGt                                                                                     <  1:3029117/150‑1 (MQ=255)

                                                       gCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTcc                                                                                   >  1:1779024/1‑150 (MQ=255)

                                                        cACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCg                                                                                  >  1:6444963/1‑150 (MQ=255)

                                                           cGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGccc                                                                               >  2:1217773/1‑150 (MQ=255)

                                                            gACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCt                                                                              <  2:1676106/150‑1 (MQ=255)

                                                             aCCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTa                                                                             >  2:1019718/1‑150 (MQ=255)

                                                             aCCAGCTGCGGGTGGCGGTTGCGACCGGGCTGATCTAAGAAGTGCTGGACAACGCACACGACTGAGCGGGCCCGGGCGGGTATCGCCATAGGCGCTATCCGCCAGGCCCCCTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTa                                                                             >  2:5919150/1‑150 (MQ=255)

                                                              actgctgctgGTGGCGGTGCCGACCGGGCAGATCAACGAAGAGCTCGACAACGCTCTCGACTGAGCGCGCCAGGCCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGATGGAGTGGCGCATAACCGTGTTGCGCTTGACCGCCCTAc                                                                            >  2:4445049/2‑150 (MQ=255)

                                                                tgcagcggGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGACAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCt                                                                          <  2:5827232/150‑1 (MQ=255)

                                                                 gctgctgGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTc                                                                         <  2:4543197/150‑1 (MQ=255)

                                                                   tgctgGTGGCGGTGGCGACCGGGCTGATCTACGAAGGGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGt                                                                       <  1:5100605/150‑1 (MQ=255)

                                                                   tgctgGGGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGt                                                                       <  2:2351418/150‑1 (MQ=255)

                                                                          ggcggtggcgACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTc                                                                <  2:7892390/150‑1 (MQ=255)

                                                                          ggcggtggcgACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTc                                                                <  2:2501600/150‑1 (MQ=255)

                                                                                 gcgACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCt                                                         <  2:5517470/150‑1 (MQ=255)

                                                                                  cgACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATTGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCtt                                                        >  1:6697925/1‑150 (MQ=255)

                                                                                   gACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTa                                                       <  1:1326965/150‑1 (MQ=255)

                                                                                    aCCGGGCTGATCTACGAAGTGCTGGACACCGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTAc                                                      <  1:8286478/150‑1 (MQ=255)

                                                                                    aCCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTAc                                                      >  2:7717322/1‑150 (MQ=255)

                                                                                       gggCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTAcccc                                                   >  2:5182111/1‑150 (MQ=255)

                                                                                        ggCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCa                                                  <  2:8461115/150‑1 (MQ=255)

                                                                                         gCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAg                                                 <  1:7792341/150‑1 (MQ=255)

                                                                                            gATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTc                                              >  1:6158873/1‑150 (MQ=255)

                                                                                               cTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAgg                                           >  2:4188988/1‑150 (MQ=255)

                                                                                               cTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAgg                                           <  1:8066583/150‑1 (MQ=255)

                                                                                                 aCGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGcc                                         <  2:9284817/150‑1 (MQ=255)

                                                                                                 aCGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGcc                                         <  1:3663085/150‑1 (MQ=255)

                                                                                                   gAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCg                                       >  1:8736272/1‑150 (MQ=255)

                                                                                                    aaGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGc                                      <  1:7449049/150‑1 (MQ=255)

                                                                                                      gTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAg                                    >  1:9975171/1‑150 (MQ=255)

                                                                                                       tGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAgg                                   <  2:1476393/150‑1 (MQ=255)

                                                                                                          tGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGt                                >  1:4724670/1‑150 (MQ=255)

                                                                                                            gACAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTg                              <  2:1856938/150‑1 (MQ=255)

                                                                                                             aCAACGCTCTCGCCTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGc                             <  2:7189515/150‑1 (MQ=255)

                                                                                                             aCAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGc                             >  2:1117312/1‑150 (MQ=255)

                                                                                                             aCAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGc                             <  1:170408/150‑1 (MQ=255)

                                                                                                             aCAACGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGc                             <  1:5549989/150‑1 (MQ=255)

                                                                                                               aaCGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAg                           <  2:3702723/150‑1 (MQ=255)

                                                                                                                aCGCTCTCGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAgg                          >  2:9715650/1‑150 (MQ=255)

                                                                                                                    tctcGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCAt                      <  2:4766368/150‑1 (MQ=255)

                                                                                                                     ctcGACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATc                     >  1:5803516/1‑150 (MQ=255)

                                                                                                                        gACTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGcc                  <  1:325639/150‑1 (MQ=255)

                                                                                                                          cTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCa                >  1:7051065/1‑150 (MQ=255)

                                                                                                                          cTGAGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCa                >  1:1664344/1‑150 (MQ=255)

                                                                                                                             aGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGtt             >  2:6393232/1‑150 (MQ=255)

                                                                                                                             aGCGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGtt             <  1:409162/150‑1 (MQ=255)

                                                                                                                               cGGGCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGTTgc           <  2:219407/150‑1 (MQ=255)

                                                                                                                                gggCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGTTgcg          <  1:5210905/150‑1 (MQ=255)

                                                                                                                                 ggCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGTTgcgc         >  1:10184247/1‑150 (MQ=255)

                                                                                                                                  gCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGTTGcgcg        <  2:3903209/150‑1 (MQ=255)

                                                                                                                                  gCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGTTGcgcg        <  2:10361153/150‑1 (MQ=255)

                                                                                                                                  gCCAGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGTTGcgcg        <  1:3346668/150‑1 (MQ=255)

                                                                                                                                     aGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGTTGCGCGgag     >  1:5386178/1‑150 (MQ=255)

                                                                                                                                     aGGGCGGGTAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCACGCAGGCGTTGCAGGGCATCGCCCAGTTGCGCGgag     >  2:4073054/1‑150 (MQ=255)

                                                                                                                                      gggcgggtAGCGCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGTTGCGCGgaga    <  1:5768403/150‑1 (MQ=255)

||

GGCCGCCTGCTAGGCCGGCTGCCGCACTACGACGGCTACGAGTGGATGCAGGCCGGCACCGACCTGCTGCTGGTGGCGGTGGCGACCGGGCTGATCTACGAAGTGCTGGACAACGCTCTCGACTGAGCGGGCCAGGGCGGGCAGGCCCATAGGCGCTATCCGCCAGGCCCCGTTGGAGTGGCGGATAACCGTGTTGCGCTTGTCCGCCCTACCTCGTGGAAGTCCTTTTCTTACCCCAGGTCAGGCCCGCAGGCGTTGCAGGGCATCGCCCAGTTGCGCGGAGAGG  >  NC_008463/2730537‑2730822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`