BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	155,993	Δ1 bp	coding (922/981 nt)	PA14_RS00700 →	ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	155,990	0	G	.	99.8%	2781.5 / ‑2.2	562	coding (919/981 nt)	PA14_RS00700	ABC transporter permease
Reads supporting (aligned to +/- strand): ref base G (0/1); new base . (126/434); total (127/435)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 578 total aligned reads displayed.

TGGCCCAGGGGATCGGGCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCCGGGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATTCTCCCAGTCGATTTGAAACAC  >  NC_008463/155841‑156136

tggCCCAGGGGATCGGGCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCCgg                                                                                                                                                   <  1:10342956/151‑1 (MQ=255)

 ggCCCAGGGGATCGGGCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCCggg                                                                                                                                                  <  2:3885005/151‑1 (MQ=255)

  gCCCAGGGGATCGGGCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCCgggc                                                                                                                                                 <  2:7891817/151‑2 (MQ=255)

     cAGGGGATCGGGCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCCgggcact                                                                                                                                              <  1:627198/151‑5 (MQ=255)

      aGGGGATCGGTCTGTCGATTCCGGCCAACCAGCAGGCGATGCTGCCCTATGCGGCGACCAACCTGGTGCTGGTCCAGCACACCCGCGATGCCCTGCGCACCCGGCTGTAAGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCCgggcacag                                                                                                                                             >  2:1809107/1‑146 (MQ=255)

      aGGGGATCGGGCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTg                                                                                                                                            <  2:4107577/151‑1 (MQ=255)

         ggATCGGGCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACg                                                                                                                                         >  1:2774639/1‑151 (MQ=255)

           aTCGGGCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAg                                                                                                                                       <  1:6472094/151‑1 (MQ=255)

            tCGGGCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAgg                                                                                                                                      <  1:6928574/151‑1 (MQ=255)

            tCGGGCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAgg                                                                                                                                      <  1:1825578/151‑1 (MQ=255)

               ggCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCa                                                                                                                                   >  2:3975145/1‑151 (MQ=255)

                gCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAg                                                                                                                                  <  2:1509159/151‑1 (MQ=255)

                gCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAg                                                                                                                                  <  1:8533939/151‑1 (MQ=255)

                 cTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAgg                                                                                                                                 <  2:10797971/151‑1 (MQ=255)

                  tGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGt                                                                                                                                <  1:1437914/151‑1 (MQ=255)

                   gTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGtt                                                                                                                               <  1:10835174/151‑1 (MQ=255)

                    tCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGttc                                                                                                                              <  2:10591878/151‑1 (MQ=255)

                    tCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGttc                                                                                                                              >  1:1504635/1‑151 (MQ=255)

                     cGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGttct                                                                                                                             <  1:7713603/151‑1 (MQ=255)

                     cGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGttct                                                                                                                             <  1:5603840/151‑1 (MQ=255)

                       aTTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGttcttc                                                                                                                           >  1:386807/1‑151 (MQ=255)

                        ttCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCg                                                                                                                          <  1:3430936/151‑1 (MQ=255)

                          ccGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGAt                                                                                                                        <  1:2412465/151‑1 (MQ=255)

                           cGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGaat                                                                                                                       >  2:55971/1‑149 (MQ=255)

                           cGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGAtt                                                                                                                       <  2:4261944/151‑1 (MQ=255)

                            ggCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTa                                                                                                                      <  1:93913/151‑1 (MQ=255)

                            ggCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTa                                                                                                                      <  1:8404455/151‑1 (MQ=255)

                            ggCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTa                                                                                                                      >  2:5350231/1‑151 (MQ=255)

                             gCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAg                                                                                                                     <  2:451273/151‑1 (MQ=255)

                               cAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAgtt                                                                                                                   >  1:4176357/1‑151 (MQ=255)

                               cAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAgtt                                                                                                                   <  1:594943/151‑1 (MQ=255)

                                 aCCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAgttgt                                                                                                                 <  1:8620537/151‑1 (MQ=255)

                                   ctgctgGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGttt                                                                                                               <  1:8899508/151‑1 (MQ=255)

                                   ctgctgGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTGTCCCTGGGCCAGCCGTGTAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGttt                                                                                                               <  2:1919362/151‑1 (MQ=255)

                                     gctgGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTa                                                                                                             <  2:3601706/151‑1 (MQ=255)

                                      ctgGCGATGCGGCCCTATGCGGCGACCAGCCTGGTGCTGGTCCGGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGGGTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTaa                                                                                                            <  2:2128166/151‑1 (MQ=255)

                                          cGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTAt                                                                                                        <  2:7082554/151‑1 (MQ=255)

                                           gATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTAtt                                                                                                       <  1:8244660/151‑1 (MQ=255)

                                           gATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTAtt                                                                                                       >  1:3609628/1‑151 (MQ=255)

                                           gATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTAtt                                                                                                       >  1:9894343/1‑151 (MQ=255)

                                             tgctgcCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTta                                                                                                     <  1:4577568/151‑1 (MQ=255)

                                              gctgcCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTtat                                                                                                    >  1:6712971/1‑151 (MQ=255)

                                              gctgcCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTtat                                                                                                    <  1:4381180/151‑1 (MQ=255)

                                               ctgcCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTtata                                                                                                   <  2:1022961/151‑1 (MQ=255)

                                               ctgcCCTAAGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTtata                                                                                                   <  2:8527631/151‑1 (MQ=255)

                                                tgcCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTtatat                                                                                                  >  2:10958934/1‑151 (MQ=255)

                                                tgcCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTtatat                                                                                                  <  1:791711/151‑1 (MQ=255)

                                                tgcCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTtatat                                                                                                  <  1:303336/151‑1 (MQ=255)

                                                 gcCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTtatata                                                                                                 >  1:1061859/1‑151 (MQ=255)

                                                   ccTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATAtt                                                                                               <  1:2170301/151‑1 (MQ=255)

                                                    cTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCTCGATGCCCTGCGCAACCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GTGCACTGACGTGGGCTGGTTCTTCGATTAGTTGTTTTAACTATTTATATAttc                                                                                              <  2:2903916/151‑1 (MQ=255)

                                                    cTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATAttc                                                                                              <  1:1324238/151‑1 (MQ=255)

                                                    cTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATAttc                                                                                              <  2:9547721/151‑1 (MQ=255)

                                                      aTGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATAttctt                                                                                            <  2:9433533/151‑1 (MQ=255)

                                                        gcggcgACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCg                                                                                          <  1:5479615/151‑1 (MQ=255)

                                                         cggcgACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGa                                                                                         <  2:6168517/151‑1 (MQ=255)

                                                          ggcgACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGAt                                                                                        <  2:5428809/151‑1 (MQ=255)

                                                          ggcgACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGAt                                                                                        <  2:374197/151‑1 (MQ=255)

                                                           gcgACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATa                                                                                       >  1:5372568/1‑151 (MQ=255)

                                                            cgACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAg                                                                                      <  2:1728548/151‑1 (MQ=255)

                                                             gACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCGGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGa                                                                                     <  2:7143107/151‑1 (MQ=255)

                                                                      ggTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATaa                                                                            <  1:5048208/151‑1 (MQ=255)

                                                                         gCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAAttt                                                                         <  1:7166572/151‑1 (MQ=255)

                                                                           tGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCa                                                                       <  1:10293608/151‑1 (MQ=255)

                                                                            tgTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGGCAGCCGTTGTAGGCC‑GGGCACTGTCGTGGGCAGGTTCTTCGATTAGTTTTTTTAACTTTTTATATATTCTTCGATAGATTTAGATAATTTCAc                                                                      <  2:8082225/150‑1 (MQ=255)

                                                                             gTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACt                                                                     <  2:9058678/151‑1 (MQ=255)

                                                                               ccTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTAGGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATGCTTCGATAGATTTAGATAATTTCACTGa                                                                   >  2:4637305/1‑151 (MQ=255)

                                                                                 tGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTAGTTATATATTCTTCGATAGATTTAGATAATTTCACTGATg                                                                 >  1:3093616/1‑151 (MQ=255)

                                                                                      tcCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTa                                                            <  2:3170863/151‑1 (MQ=255)

                                                                                      tcCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTa                                                            <  2:5130316/151‑1 (MQ=255)

                                                                                       cccGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTaa                                                           <  2:8397549/151‑1 (MQ=255)

                                                                                        ccGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTaaa                                                          <  2:6259352/151‑1 (MQ=255)

                                                                                         cgcgATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAAt                                                         >  1:10030080/1‑151 (MQ=255)

                                                                                           cgATGCGCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGTTAGTCC‑GGGCAATGAAGAGGGTAGGTTCTTCGAATTTTTGTTTTAACTATTTATATATTCTTGGTTAGATTTAGATCATTTCACTGATGGCCTAAATCa                                                       <  2:6904126/151‑1 (MQ=255)

                                                                                            gATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATCTATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCaa                                                      >  2:250072/1‑151 (MQ=255)

                                                                                            gATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATCTATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCaa                                                      >  2:1173721/1‑151 (MQ=255)

                                                                                            gATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCaa                                                      >  2:6447834/1‑151 (MQ=255)

                                                                                             aTGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAAt                                                     <  2:982593/151‑1 (MQ=255)

                                                                                              tGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATg                                                    <  2:8784737/151‑1 (MQ=255)

                                                                                              tGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATg                                                    <  1:1984704/151‑1 (MQ=255)

                                                                                                cccTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATggg                                                  <  1:9028251/151‑1 (MQ=255)

                                                                                                cccTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTACGATTAGTTGTTTTCACTATTTATATATTCTTCGATAGATTTAGATAATTTAACTGATGGCCTAAATCAATggg                                                  >  2:4386972/1‑151 (MQ=255)

                                                                                                   tGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCa                                               <  1:8970929/151‑1 (MQ=255)

                                                                                                     cgcACCCGGCTGTATGCCCCGGTGTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCAtt                                             <  2:1363159/151‑1 (MQ=255)

                                                                                                            ggCTGTATGCCCCGGATTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTCTATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCg                                      <  2:5750627/151‑1 (MQ=255)

                                                                                                             gCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGa                                     <  1:9499775/151‑1 (MQ=255)

                                                                                                                gTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAAcc                                  <  1:683979/151‑1 (MQ=255)

                                                                                                                        cGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAg                          <  1:10769550/151‑1 (MQ=255)

                                                                                                                        cGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAg                          >  1:6056493/1‑151 (MQ=255)

                                                                                                                            ttCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATtc                      <  2:4074127/151‑1 (MQ=255)

                                                                                                                             tCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATtct                     <  1:5677518/151‑1 (MQ=255)

                                                                                                                                cTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATTCTccc                  <  1:3298234/151‑1 (MQ=255)

                                                                                                                                 tGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATTCTCCCa                 >  1:3550324/1‑151 (MQ=255)

                                                                                                                                 tGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATTCTCCCa                 <  2:2720234/151‑1 (MQ=255)

                                                                                                                                    ggcAGCCGTGGTAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTTGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATTCTCCCAGTc              <  2:9122200/149‑1 (MQ=255)

                                                                                                                                     ccAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATTCTCCCAGTCg             >  2:8216312/1‑151 (MQ=255)

                                                                                                                                         ccGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATTCTCCCAGTCGAttt         <  1:9650546/151‑1 (MQ=255)

                                                                                                                                          cGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATTCTCCCAGTCGATTTg        >  1:7472149/1‑151 (MQ=255)

                                                                                                                                             ggAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATTCTCCCAGTCGATTTGaaa     <  2:7461306/151‑1 (MQ=255)

                                                                                                                                              gAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATTCTCCCAGTCGATTTGAAac    <  2:665127/151‑1 (MQ=255)

TGGCCCAGGGGATCGGGCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCCGGGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATTCTCCCAGTCGATTTGAAACAC  >  NC_008463/155841‑156136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`