BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	3,670,543	+A	coding (543/1020 nt)	PA14_RS16675 →	ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	3,670,542	1	.	A	99.3%	1712.8 / 2.2	408	coding (542/1020 nt)	PA14_RS16675	ABC transporter permease
Reads supporting (aligned to +/- strand): ref base . (0/3); new base A (198/206); total (198/210)
Fisher's exact test for biased strand distribution p-value = 2.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 420 total aligned reads displayed.

GACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCGTTTTCCTGG‑A‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTTCCTCCGCGGG  >  NC_008463/3670391‑3670687

gACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGTGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTgg                                                                                                                                                     >  1:1317186/1‑151 (MQ=255)

 aCGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGG‑a                                                                                                                                                   <  1:6688546/151‑1 (MQ=255)

 aCGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGG‑a                                                                                                                                                   <  2:6758167/151‑1 (MQ=255)

     gCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGG‑aagtc                                                                                                                                               >  1:617321/1‑147 (MQ=255)

         gCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGt                                                                                                                                           <  2:9215427/151‑1 (MQ=255)

           ccGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCg                                                                                                                                         >  1:9276589/1‑151 (MQ=255)

              ggTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCgggg                                                                                                                                      <  1:3723487/151‑1 (MQ=255)

                tGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGGGCTCAGTTCTCTGATCGGCGTCACCGT‑CGGCGCCCTGCACGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGG‑ACGTCTGGTCGGGGCt                                                                                                                                    <  2:4831947/151‑1 (MQ=255)

                 gATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTg                                                                                                                                   <  1:1659077/151‑1 (MQ=255)

                 gATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTg                                                                                                                                   >  1:5947572/1‑151 (MQ=255)

                   ttttCGGCTTCCGCGTGTCGATCCTCATCGCCCTGTCCGTGACCGTGCTCTGTTCACTGATCGGCGTCACCTT‑AGGCGCCCTGCAAGTCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGcc                                                                                                                                 <  2:5481327/151‑1 (MQ=255)

                   tttACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGcc                                                                                                                                 >  1:9811839/1‑151 (MQ=255)

                      ccgcccaccGCGTGTCGATCCTCTACGCCCTGACCCTGCCCCCGCTCAGTTCACTGGTGTGCGTCCCAGTGCCGTGTCCT‑CTTGGGTTCTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGt                                                                                                                              <  1:5200749/144‑1 (MQ=255)

                          cTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGctc                                                                                                                          >  1:87039/1‑151 (MQ=255)

                           ttCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑ATTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGctct                                                                                                                         >  1:10448353/1‑151 (MQ=255)

                            tCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTa                                                                                                                        >  1:4027631/1‑151 (MQ=255)

                             ccGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGctcttc                                                                                                                       <  1:6312624/151‑3 (MQ=255)

                              cgcgTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTAcc                                                                                                                      <  1:10031767/151‑1 (MQ=255)

                               gcgTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACct                                                                                                                     <  1:8542847/151‑1 (MQ=255)

                                 gtgtCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACctgc                                                                                                                   <  2:1193157/151‑1 (MQ=255)

                                 gtgtCGATCAACTTCGCCCTGACCAAGACCGAGCTCAGTTCTCTGATCGGCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACctgc                                                                                                                   >  2:573060/1‑151 (MQ=255)

                                        tCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGatcat                                                                                                            >  2:9436926/1‑151 (MQ=255)

                                        tCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGatcat                                                                                                            >  2:8283457/1‑151 (MQ=255)

                                           tctTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCt                                                                                                         >  2:232949/1‑151 (MQ=255)

                                            ctTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTg                                                                                                        >  1:5413851/1‑151 (MQ=255)

                                             ttCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTgg                                                                                                       >  2:7863147/1‑151 (MQ=255)

                                             ttCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTgg                                                                                                       >  1:8981048/1‑151 (MQ=255)

                                               cGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGcc                                                                                                     <  1:8084572/151‑1 (MQ=255)

                                                gCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCa                                                                                                    <  2:1505305/151‑1 (MQ=255)

                                                 ccctgaccctgaCCGTGCTCAGTTCTCTGATCGGCGTCACCGT‑CGGCGCCCTGCAAGGATACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTGGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAg                                                                                                   >  2:9356121/1‑151 (MQ=255)

                                                  cctgaccctgacCGTGCTCAGTTCTCTGATCGGCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGc                                                                                                  >  1:1500544/1‑151 (MQ=255)

                                                  cctgaccctgacCGTGCTCAGTTCTCTGATCGGCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGc                                                                                                  >  1:2746897/1‑151 (MQ=255)

                                                   ctgaccctgaccGTGCTCAGTTCTCTGATCGGCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCt                                                                                                 <  1:2006292/151‑1 (MQ=255)

                                                     gaccctgaccGTGCTCAGTTCTCTGATCGGCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTc                                                                                               <  1:11112952/151‑1 (MQ=255)

                                                      accctgaccGTGCTCAGTTCTCTGATCGGCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCg                                                                                              >  1:4585416/1‑151 (MQ=255)

                                                          tgaccGTGCTCAGTTCTCTGATCGGCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCa                                                                                          <  2:6507973/151‑1 (MQ=255)

                                                           gaccGTGCTCAGTTCTCTGATCGGCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAg                                                                                         <  2:4653488/151‑1 (MQ=255)

                                                            accGTGCTCAGTTCTCTGATCGGCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGc                                                                                        >  1:3230150/1‑151 (MQ=255)

                                                              cGTGCTCAGTTCTCTGATCGGCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCg                                                                                      <  1:7861802/151‑1 (MQ=255)

                                                              cGTGCTCAGTTCTCTGATCGGCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCAGCCTGGCCAGCTTCGTCCAGCCg                                                                                      <  2:3963228/151‑1 (MQ=255)

                                                                tGCTCAGTTCTCTGATCGGCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGaa                                                                                    >  2:9825/1‑151 (MQ=255)

                                                                   tCAGTTCTCTGATCGGCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACtt                                                                                 >  1:6069989/1‑151 (MQ=255)

                                                                   tCAGTTCTCTGATCGGCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACtt                                                                                 <  2:3882439/151‑1 (MQ=255)

                                                                    cAGTTCTCTGATCGGCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTc                                                                                >  1:10258509/1‑151 (MQ=255)

                                                                     aGTTCTCTGATCGGCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCt                                                                               <  2:6907548/151‑1 (MQ=255)

                                                                      gTTCTCTGATCGGCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCtg                                                                              <  1:10832315/151‑1 (MQ=255)

                                                                      gTTCTCTGATCGGCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCtg                                                                              <  2:1065933/151‑1 (MQ=255)

                                                                       ttCTCTGATCGGCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCtgg                                                                             >  2:2804914/1‑151 (MQ=255)

                                                                       ttCTCTGATCGGCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCtgg                                                                             <  2:5265111/151‑1 (MQ=255)

                                                                        tctcTGATCGGCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCtggt                                                                            <  1:4075882/151‑1 (MQ=255)

                                                                            tGATCGGCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGgct                                                                        <  1:9702314/151‑1 (MQ=255)

                                                                            tGATCGGCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGgct                                                                        <  1:4448318/151‑1 (MQ=255)

                                                                               tCGGCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGgctgct                                                                     >  1:4300392/1‑151 (MQ=255)

                                                                                cGGCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGctgctg                                                                    <  1:2519282/151‑1 (MQ=255)

                                                                                 ggCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCTTCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTgg                                                                   <  1:5695584/151‑1 (MQ=255)

                                                                                 ggCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTgg                                                                   <  1:6523628/151‑1 (MQ=255)

                                                                                  gCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTggg                                                                  <  1:8208315/151‑1 (MQ=255)

                                                                                   cGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGc                                                                 <  2:6083361/151‑1 (MQ=255)

                                                                                   cGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TGTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGc                                                                 <  2:6215448/151‑1 (MQ=255)

                                                                                      cACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGcatc                                                              >  2:3708846/1‑151 (MQ=255)

                                                                                             cGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCAtgctg                                                        <  1:3832176/151‑1 (MQ=255)

                                                                                               gcgcCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATgctgct                                                      <  1:5825150/151‑1 (MQ=255)

                                                                                                 gcCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGt                                                    <  1:2318899/151‑1 (MQ=255)

                                                                                                    cTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGtttt                                                 >  1:1779699/1‑151 (MQ=255)

                                                                                                      gCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTcc                                               <  1:748183/151‑1 (MQ=255)

                                                                                                       cAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCt                                              <  1:2195456/151‑1 (MQ=255)

                                                                                                        aaGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTg                                             <  1:8795062/151‑1 (MQ=255)

                                                                                                          ggCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGa                                           <  2:11004766/151‑1 (MQ=255)

                                                                                                          ggCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGa                                           <  1:2921742/151‑1 (MQ=255)

                                                                                                           gCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGAt                                          <  1:7820742/151‑1 (MQ=255)

                                                                                                                tacGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGcc                                     >  2:8502552/1‑151 (MQ=255)

                                                                                                                 acGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCt                                    >  2:2599499/1‑151 (MQ=255)

                                                                                                                   ggcggcTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCtgg                                  <  2:4244265/151‑1 (MQ=255)

                                                                                                                     cggcTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCtggtg                                <  2:8619983/151‑1 (MQ=255)

                                                                                                                      ggcTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCtggtgg                               <  1:7738043/151‑1 (MQ=255)

                                                                                                                      ggcTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCtggtgg                               >  2:2143688/1‑151 (MQ=255)

                                                                                                                        cTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGTA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGAc                             <  2:886747/151‑1 (MQ=255)

                                                                                                                        cTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGAc                             <  1:3386899/151‑1 (MQ=255)

                                                                                                                         tGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTTCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACg                            >  2:5983160/1‑151 (MQ=255)

                                                                                                                         tGGGTCGACCTGCTCGGCCAGCG‑TGTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACg                            <  2:3041537/151‑1 (MQ=255)

                                                                                                                            gTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTgg                         >  1:9395002/1‑151 (MQ=255)

                                                                                                                            gTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTgg                         >  1:6990221/1‑151 (MQ=255)

                                                                                                                             tCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGt                        >  2:1923238/1‑151 (MQ=255)

                                                                                                                                aCCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCcg                     >  2:336331/1‑151 (MQ=255)

                                                                                                                                aCCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCcg                     >  2:7586947/1‑151 (MQ=255)

                                                                                                                                aCCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCcg                     <  1:3578629/151‑1 (MQ=255)

                                                                                                                                    gCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGcc                 >  2:10172858/1‑151 (MQ=255)

                                                                                                                                     cTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCg                <  1:9929725/151‑1 (MQ=255)

                                                                                                                                      tCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGa               <  2:7341350/151‑1 (MQ=255)

                                                                                                                                      tCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGa               >  1:7234800/1‑151 (MQ=255)

                                                                                                                                      tCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGa               >  2:4941924/1‑151 (MQ=255)

                                                                                                                                      tCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTCGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGa               >  2:10533377/1‑151 (MQ=255)

                                                                                                                                         gCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGtt            <  1:8456307/151‑1 (MQ=255)

                                                                                                                                           cAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTtcc          >  2:8183294/1‑151 (MQ=255)

                                                                                                                                             agcgtttCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTtcct         >  2:5090164/5‑151 (MQ=255)

                                                                                                                                             agcgtttCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTtcct         <  2:9128112/147‑1 (MQ=255)

                                                                                                                                              gcgtttCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTtcctc        <  2:7767555/148‑1 (MQ=255)

                                                                                                                                              gcgtttCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTtcctc        <  1:10194979/148‑1 (MQ=255)

                                                                                                                                              gcgtttCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCGCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTtcctc        >  1:10736833/4‑151 (MQ=255)

                                                                                                                                                 tttCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTTCCTCcgc     >  1:4772581/1‑151 (MQ=255)

GACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGT‑CGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCGTTTTCCTGG‑A‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTTCCTCCGCGGG  >  NC_008463/3670391‑3670687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`