Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084635,248,9500TG22.4% 882.1 / 10.8 351L666R (CTG→CGG) tpsA4two‑partner secretion system exoprotein TpsA4
Reads supporting (aligned to +/- strand):  ref base T (50/192);  new base G (66/4);  total (155/196)
Fisher's exact test for biased strand distribution p-value = 1.68e-30
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 359 total aligned reads displayed.

GGAGCAGCGAGAATGTCGGTTCCGGCCTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGG‑C‑AACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGG‑CCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCAGCGCCCTCTCCGTATCGACGACAGCCTCTCTCTCAC  >  NC_008463/5248800‑5249100
                                                                                                                                                      |                                                                                                                                                         
      gcGAGAATGTCGGTTCCGGCCTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTg                                                                                                                                                     >  2:5091690/1‑151 (MQ=255)
       cGAGAAGGTCGGTTCCGGCCTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATCCCCTGGCCCGCACCCTGTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGGCGCTGGCGTgg                                                                                                                                                    >  2:5643510/1‑151 (MQ=255)
         agaATGTCGGTTCCGGCCTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGGCGCGGTGCggggg                                                                                                                                                  >  1:3519837/1‑150 (MQ=255)
          gaATGTCGGTTCCGGCCTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGGCGCGGTGCgggggc                                                                                                                                                 >  1:7490524/1‑149 (MQ=255)
            aTGTCGGTTCCGGCCTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAg                                                                                                                                               <  1:5852540/151‑1 (MQ=255)
            aTGTCGGTTCCGGCCTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAg                                                                                                                                               <  2:3722780/151‑1 (MQ=255)
            aTGTCGGTTCCGGCCTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCGGTCCAGGGACAg                                                                                                                                               >  1:833455/1‑151 (MQ=255)
               tCGGTTCCGGCCTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCGGTCCTGGGACAGCgg                                                                                                                                            >  1:3057886/1‑151 (MQ=255)
                cGGGTCCGGCCTGGCGAATCCACGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGG‑c                                                                                                                                          <  2:1421979/151‑1 (MQ=255)
                  gTTCCGGCCTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGG‑C‑aa                                                                                                                                       <  1:9160115/151‑1 (MQ=255)
                  gTTCCGGCCTGGCGAATCCAAGCAGCGCGGGAAAAGCTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTGTCCACACGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGG‑C‑aa                                                                                                                                       <  1:7830644/151‑1 (MQ=255)
                   gtCCGGCCTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGG‑C‑AAc                                                                                                                                      <  1:3767901/150‑1 (MQ=255)
                    tCCGGCCTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGGCGCGGTCCGGGGGCAGCGG‑C‑CACg                                                                                                                                     >  1:6169773/1‑151 (MQ=255)
                    tCCGGCCTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGG‑C‑AACg                                                                                                                                     <  1:2413222/151‑1 (MQ=255)
                    tCCGGCCTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGG‑C‑AACg                                                                                                                                     <  1:3309284/151‑1 (MQ=255)
                    tCCGGCCTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCCCGGTCCTGGGACCGCGG‑C‑AACg                                                                                                                                     >  2:6628826/1‑151 (MQ=255)
                     ccGGCCTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCGGTGGGGGGACAGCGG‑G‑AACgg                                                                                                                                    >  1:9923174/1‑151 (MQ=255)
                      cGGCCTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGG‑C‑AACGGc                                                                                                                                   <  1:5654395/151‑1 (MQ=255)
                         ccTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGGCGCGGTCCTGGGGCCGCGG‑C‑AACGGccag                                                                                                                                >  1:5076020/1‑149 (MQ=255)
                         ccTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCGGTACGGGGACAGCGG‑C‑AACGGccag                                                                                                                                >  1:8015253/1‑149 (MQ=255)
                         ccTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCCCAGTGCAGGGTCAGCGG‑C‑CACCGCCTg                                                                                                                                >  1:647116/1‑151 (MQ=255)
                            ggCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCCCAGTGGTGGGGcaacggcc‑aacggcgggcg                                                                                                                              >  1:9749894/1‑146 (MQ=255)
                              cGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGGCGCGGTGCGGGGGGCGCGG‑C‑AAACGCCGGACGCt                                                                                                                           >  1:903182/1‑151 (MQ=255)
                              cGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGGCGCAGTGCGGGGTCCGGGG‑C‑AACCGCCTGTCGCt                                                                                                                           >  1:10126777/1‑151 (MQ=255)
                                aaTCCTAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGG‑C‑AACGGCCTGACGCTaa                                                                                                                         <  2:7330637/151‑1 (MQ=255)
                                 aTCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCCCAGTGCGGGGACACCGG‑C‑CACCGCCTGAAGCGAAc                                                                                                                        >  2:8385408/1‑151 (MQ=255)
                                  tCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGG‑C‑AACGGCCTGACGCTAAcc                                                                                                                       <  1:5463651/151‑1 (MQ=255)
                                  tCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCGGTCCGGGGACAGCGG‑C‑CACGGCCTGAAGCTaaac                                                                                                                       >  2:4251812/1‑149 (MQ=255)
                                     aaGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGGCGCGGTCCAGGGACAGCGG‑G‑CACCGCCCGACGCGaaacccc                                                                                                                    >  1:8775479/1‑146 (MQ=255)
                                     aaGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGG‑C‑AACGGCCTGACGCTAAccgcc                                                                                                                    <  1:8535169/151‑1 (MQ=255)
                                       gcagcGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCAGTCCGGGGACAGCCG‑C‑AAAGGCCAGACGCTAACCCCCGa                                                                                                                  >  2:10768222/1‑151 (MQ=255)
                                           cgcgGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGGCCGGGTGCAGGGGCAGCGG‑G‑AAAGGCCGGACGCGAAACGCCCAgcgc                                                                                                              >  1:2718405/1‑151 (MQ=255)
                                             cgGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACCGCGG‑C‑AACGGCCAGACGCGAACCGCCGAGCGCgg                                                                                                            >  2:9615651/1‑151 (MQ=255)
                                               ggAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGG‑C‑AACGGCCTGACGCTAACCGCCGAGCGCgggg                                                                                                          <  2:9198548/151‑1 (MQ=255)
                                                gAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGG‑C‑AACGGCCTGACGCTAACCGCCGAGCGCGGGGa                                                                                                         <  2:7398255/151‑1 (MQ=255)
                                                 acaaGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCAACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGG‑C‑AACGGCCTGACGCTAACCGCCGAGCGCGGGGAc                                                                                                        <  1:946005/149‑1 (MQ=255)
                                                 aaaaGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTACTGGGACAGCCG‑C‑AACGGCCAGTCGCAAACCGCCGAGCGCGGGGAc                                                                                                        >  2:7674657/1‑151 (MQ=255)
                                                 aaaaGGTCCGACGCGGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGG‑C‑AACGGCCTGACGCTAACCGCCGAGCGCGGGGAc                                                                                                        <  2:6812277/151‑1 (MQ=255)
                                                    aGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGGCGCGGGCCGTTGACACCGG‑CGAACGCCCT‑ACGCGAAACCCCCACCGCGGGGccccc                                                                                                     >  1:2536208/1‑149 (MQ=255)
                                                     ggTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGG‑C‑AACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCa                                                                                                    <  2:5843151/151‑1 (MQ=255)
                                                      gTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGGCGCGGGGCGGGGGACGCGG‑C‑CACCGCCGGAGGCAAACCGCCCAGCGCGGGGGCCTCAg                                                                                                   >  1:11094257/1‑151 (MQ=255)
                                                      gTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGG‑C‑AACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAg                                                                                                   <  2:6974918/151‑1 (MQ=255)
                                                      gTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCGGGGACAGCGG‑C‑AACCGCCTGACGCAAAACCCCGAGCGCGGGGACCTCAg                                                                                                   >  2:3356066/1‑151 (MQ=255)
                                                      gTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCGGTGCTGGGACCGCGG‑G‑AACGGCCTGACGCTAACCGCCGTGCGCGGGGGCCTCAg                                                                                                   >  2:7173473/1‑151 (MQ=255)
                                                       tCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGG‑C‑AACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAgg                                                                                                  <  1:10812420/151‑1 (MQ=255)
                                                         cGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGG‑C‑AACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGAt                                                                                                <  2:3924858/151‑1 (MQ=255)
                                                          gACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCGGTCCAGGGACAGCGG‑C‑CACGGCCTGACGCAAACCGCCGAGCGCGGGGACCTCAGGAta                                                                                               >  2:10136500/1‑150 (MQ=255)
                                                           aCGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGG‑C‑AACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCa                                                                                              <  1:3809710/151‑1 (MQ=255)
                                                            cGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGG‑C‑AACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCaa                                                                                             <  1:6871682/151‑1 (MQ=255)
                                                            cGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCGGGGACCGCGG‑C‑CACGGCCTGACGCTAACCGCCGAGCGCGGGGCCCTCAGGATCaa                                                                                             >  2:5997854/1‑151 (MQ=255)
                                                              cTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGG‑C‑AACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATg                                                                                           <  1:2225493/151‑1 (MQ=255)
                                                               tGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCGGTCCAGGGCCAGCGG‑C‑AAAGGCCTGACCCTAACCGCCGAGCGCGGGGACCTCAGGATAAATgg                                                                                          >  2:10390776/1‑151 (MQ=255)
                                                                gCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCCCAGTGCGGGGACCGCGG‑C‑CACCGCCAGACGCGAACCCCCCCGCGCGGGGCCCGCAGGGTCAATGgc                                                                                         >  2:9789325/1‑151 (MQ=255)
                                                                  aTGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCAGTCCTGGGACAGCGG‑C‑AACGGCCTGACGCTAACCCCCGAGCGCGGGGACCACAGGATCAATGgcgc                                                                                       >  2:6145973/1‑151 (MQ=255)
                                                                    gCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGGCGCTGTCCAGGGACAGCGG‑C‑AACGGCCAGAAGCGAACCCCCGAGCGCGGGGACCGCCCGATCAAAGgcgcgc                                                                                     >  2:5869994/1‑151 (MQ=255)
                                                                     ccGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCAGGGAAACCGG‑C‑CAAGGGCAGACGCAAACCGCCGAGCGCGGGGTCCACAGGATAAAAGGCGCGCt                                                                                    >  2:8905967/1‑151 (MQ=255)
                                                                       gATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCGGTGCAGGGGCCCCGG‑G‑CACGGCCAGACGCAAACCGCCGCGCGCGGGGGCCCCCGGGGAAAAGGCGCGCGGa                                                                                  >  1:10901405/1‑151 (MQ=255)
                                                                        aTACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGG‑C‑AACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGAc                                                                                 <  2:4797308/151‑1 (MQ=255)
                                                                             cTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGGCGCGGGCCAGGGGCAGCGG‑C‑AACCGCCAGACGCGAAACCCCCCGCGCGGGGACCGCAGGATCAAAGGCGCGCGGGCCG‑ccc                                                                           >  2:445225/1‑151 (MQ=255)
                                                                               ggCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGG‑C‑AACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGG‑CCCAg                                                                         <  2:1254851/151‑1 (MQ=255)
                                                                                gCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGG‑C‑AACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGG‑CCCAgg                                                                        <  1:1309722/151‑1 (MQ=255)
                                                                                 cccGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCGGTCCCGGGGCAGCGG‑C‑AACCGCCAGACGCAAAACCCCCAGCCCGGGGGCCACAGGATCAAAGGCGCGCTGACGG‑CCCCggg                                                                       >  2:1063774/1‑151 (MQ=255)
                                                                                 cccGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCAGTGCTGGGACCGCGG‑C‑AACGGCCTGACGCAAACCGCCCAGCGCCGGGACCACATGATCAATAGCGCGCAGACGG‑CCCAcgg                                                                       >  1:5385147/1‑151 (MQ=255)
                                                                                  ccGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCGGTCCTGGGTCAGCGG‑C‑AACGGCCGGACGCGAAACGCCGAGCGCGGGGACCACAGGATCAAAGGCGCGCTGACGG‑CCCAgggg                                                                      >  2:5411559/1‑151 (MQ=255)
                                                                                   cGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGGCGCGGTGCGGGGCCAGCGG‑C‑AACCGCCCGACCCGAACCCCCCAGGGCGGGGGCCGCCGGATCAAATGCGCGCGGGCGG‑CCCAggggg                                                                     >  2:1967720/1‑151 (MQ=255)
                                                                                    gCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGG‑C‑AACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGG‑CCCAGGGGGa                                                                    <  2:5476335/151‑1 (MQ=255)
                                                                                     cAACCTTTCCACCAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGG‑C‑AACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGG‑CCCAGGGGGaa                                                                   <  2:5754797/151‑1 (MQ=255)
                                                                                     cAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGG‑C‑AACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGG‑CCCAGGGGGaa                                                                   <  1:1615853/151‑1 (MQ=255)
                                                                                       aCCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGG‑C‑AACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGG‑CCCAGGGGGaaaa                                                                 <  2:1855430/151‑1 (MQ=255)
                                                                                       aCCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCCCGGTCCAGGGACAGCGG‑C‑AAAGGCCAGACGCAAACCGCCCCGCGCGGGGACCTCAGGATCAATGGCGCGCAGACGG‑CCCAGGGGGaaaa                                                                 >  2:8100341/1‑151 (MQ=255)
                                                                                           ttCCACAAGCAACGTCGAAGTGGTCGCCAACCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGAAAGCGG‑C‑AACTGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGG‑CCCAGGGGGATATCGcc                                                             <  2:7936139/151‑1 (MQ=255)
                                                                                                caAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCGCCTGTCCTGGGACAGCGG‑C‑AACGGCCGGACGCGAACCGCCGAGCGCGGGGGCCTCACGAGAAAATGCGCGCTGTCGG‑GCCAGGGGGGCAACGCCAGccg                                                        >  2:3165711/1‑150 (MQ=255)
                                                                                                      aCGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGG‑C‑AACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGG‑CCCAGGGGGAAAACGCCAGCCTTActct                                                  <  2:3057886/151‑1 (MQ=255)
                                                                                                       cGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTGCTGGGGCAGCGG‑C‑AACGGCCGGACGCGAACCGCCGAGCGCGGGGACCACAGGATCAAAAGCGCGCTGTCGG‑CCCCCGGGGGAAACGCCAGCCTTACtctc                                                 >  2:10861721/1‑151 (MQ=255)
                                                                                                       cGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCGGTCCTGGGACAGCGG‑C‑AACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGG‑CCCAGGGGGAAAACGCCAGCCTTACtctc                                                 <  2:817321/151‑1 (MQ=255)
                                                                                                           gAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGG‑C‑AACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGG‑CCCAGGGGGAAAACGCCAGCCTTACTCTCAATg                                             <  2:1773940/151‑1 (MQ=255)
                                                                                                            aaGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGG‑C‑AACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGG‑CCCAGGGGGAAAACGCCAGCCTTACTCTCAATGc                                            <  2:10474164/151‑1 (MQ=255)
                                                                                                             aGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGG‑C‑AACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGG‑CCCAGGGGGAAAACGCCAGCCTTACTCTCAATGcc                                           <  2:7026688/151‑1 (MQ=255)
                                                                                                                  tCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGG‑C‑AACGGCCTGACGCTAACCGGCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGG‑CCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCa                                      <  1:458157/151‑1 (MQ=255)
                                                                                                                   cGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCGGTCCTGGGACAGCGG‑C‑AACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGG‑CCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCAg                                     <  2:6286438/151‑1 (MQ=255)
                                                                                                                    gCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGG‑C‑AACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGG‑CCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCAgc                                    <  1:6794847/151‑1 (MQ=255)
                                                                                                                    gCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGG‑C‑AACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGG‑CCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCAgc                                    <  1:4336707/151‑1 (MQ=255)
                                                                                                                      cACCCGGGGCAACGCGCATGTCGGCGCGGCGCGGTGCTGGGCCAGCGG‑C‑AACGGCCAGACGCTAACCCCCCAGCGCGGGGACCACAGGAAAAATGTCGCGCTGACGGCCCCAGGGGGAAAA‑GCCAGCCATTAACTCCATGCCGGGCAgcgg                                  >  2:775707/1‑150 (MQ=255)
                                                                                                                       aCCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGG‑C‑AACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGG‑CCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCAGCGcc                                 <  1:10262980/151‑1 (MQ=255)
                                                                                                                       aCCCGGGGCAACGCGCATGTCGGCGCGCCGCGGTGCAGGGACCGCGG‑C‑CACCGCCAGAAGCGAACCCCCCAGCGCGGGGACCACAGGATCAAAGGCGCGCAGACGG‑CCCAGGGGGGAAAAGCCCCCCATACACACAATGCCGGGCCGCGcc                                 >  2:6371000/1‑151 (MQ=255)
                                                                                                                           ggggCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGG‑C‑AACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGG‑CCCAGGGGGAAAACGCCAGCCTTACTCTCACTGCCGGGCAGCGCCctct                             <  2:2761690/151‑1 (MQ=255)
                                                                                                                           ggggCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGG‑C‑AACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGG‑CCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCAGCGCCctct                             <  2:2493480/151‑1 (MQ=255)
                                                                                                                                aaCGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGG‑C‑AACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGG‑CCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCAGCGCCCTCTCCGTa                        <  2:9510096/151‑1 (MQ=255)
                                                                                                                                 aCGCGCATGTCGGCGCGCCGCGGTGCAGGGGCACCGG‑C‑CACGGCCAGACGCAAAACCCCGAGCGCGGGGACCGCCGGGTAAAATGCGCGCGGTCGG‑CCCCGGGGGTAAACCCCCGCCATAAAAGCAAAGCCGGGCCGCCCCCGCACCGGAt                       >  2:4167277/1‑151 (MQ=255)
                                                                                                                                       aTGTCGGCGCGCCGCTGTCCTGGGACAGCGG‑C‑AACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGG‑CCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCAGCGCCCTCTCCGTATcgacga                 <  2:7805267/151‑1 (MQ=255)
                                                                                                                                        tGTCGGCGCGCCGCTGTCCTGGGACAGCGG‑C‑AACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGG‑CCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCAGCGCCCTCTCCGTATCgacgac                <  1:1472953/151‑1 (MQ=255)
                                                                                                                                        tGTCGGCGCGCCGCTGTCCTGGGACAGCGG‑C‑AACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGG‑CCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCAGCGCCCTCTCCGTATCgacgac                <  1:8194233/151‑1 (MQ=255)
                                                                                                                                         gTCGGCGCGCCGCTGTCCTGGGACAGCGG‑C‑AACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGG‑CCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCAGCGCCCTCTCCGTATCGACGACa               <  1:6627210/151‑1 (MQ=255)
                                                                                                                                             gcgcgcCGCTGTCCTGGGACAGCGG‑C‑AACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGG‑CCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCAGCGCCCTCTCCGTATCGACGACAGCct           >  2:4045017/1‑151 (MQ=255)
                                                                                                                                              cgcgcCGCTGTCCTGGGACAGCGG‑C‑AACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGG‑CCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCAGCGCCCTCTCCGTATCGACGACAGCctc          >  1:2889987/1‑151 (MQ=255)
                                                                                                                                                 gccgcTGTCCTGGGACAGCGG‑C‑AACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGG‑CCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCAGCGCCCTCTCCGTATCGACGACAGCctctct       >  1:478589/1‑151 (MQ=255)
                                                                                                                                                  ccgcTGTCCTGGGACAGCGG‑C‑AACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGG‑CCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCAGCGCCCTCTCCGTATCGACGACAGCctctctc      >  1:4161185/1‑151 (MQ=255)
                                                                                                                                                   cgcTGTCCTGGGACAGCGG‑C‑AACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGG‑CCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCAGCGCCCTCTCCGTATCGACGACAGCctctctct     >  1:4014395/1‑151 (MQ=255)
                                                                                                                                                   cgcTGTCCTGGGACAGCGG‑C‑AACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGG‑CCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCAGCGCCCTCTCCGTATCGACGACAGCctctctct     <  2:11083502/151‑1 (MQ=255)
                                                                                                                                                      tGTCCTGGGACAGCGG‑C‑AACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGG‑CCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCAGCGCCCTCTCCGTATCGACGACAGCCTCTCTCTCAc  >  2:8772119/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                         
GGAGCAGCGAGAATGTCGGTTCCGGCCTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGG‑C‑AACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGG‑CCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCAGCGCCCTCTCCGTATCGACGACAGCCTCTCTCTCAC  >  NC_008463/5248800‑5249100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑