Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,423,281 A→G K130K (AAA→AAG)  mexY → multidrug efflux RND transporter permease subunit MexY

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,423,2810AG99.1% 1457.5 / NA 446K130K (AAA→AAG) mexYmultidrug efflux RND transporter permease subunit MexY
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (226/216);  minor base T (4/0);  total (230/216)
Fisher's exact test for biased strand distribution p-value = 1.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 449 total aligned reads displayed.

CCAGCAGCACCGGCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAAGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCCAGACCTGGGGCGC  >  NC_008463/3423133‑3423427
                                                                                                                                                    |                                                                                                                                                  
 cagcagCACCGGCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGgcg                                                                                                                                                 >  2:7963147/1‑151 (MQ=255)
  agcagcACCGGCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGgcgg                                                                                                                                                >  2:10139095/1‑151 (MQ=255)
  agcagcACCGGCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGgcgg                                                                                                                                                <  2:3369046/151‑1 (MQ=255)
     agcaCCGGCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCGACGTGGAGAAGGcggcgg                                                                                                                                             >  2:591606/1‑151 (MQ=255)
      gcaCCGGCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGa                                                                                                                                            <  2:7735904/151‑1 (MQ=255)
       caCCGGCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGGCGCGCCAGCCCGGGTCGGTGCGGCGCGACGGGATCTACGGGGTGAAGGCGGCGGAc                                                                                                                                           >  1:4809926/1‑151 (MQ=255)
        aCCGGCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACa                                                                                                                                          <  2:3173535/151‑1 (MQ=255)
         ccGGCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAg                                                                                                                                         >  2:4835922/1‑151 (MQ=255)
          cGGCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGGGGAGAAGGCGGCGGACAGc                                                                                                                                        >  2:86581/1‑151 (MQ=255)
            gCCAGGCTTCGCTGCCCCTGACCTTCCGCCAGGGCGTGAACCCCGACCGCGCCGCGTGGGAAGAGCATAACCGCCTGAATCTAGGCGACTCGCTCATGCGCGAGGCAGTGCGGCGCGATGGTATCTAAGATGAGAAGGAGGAGGACAGctt                                                                                                                                      >  2:7179722/1‑149 (MQ=255)
            gCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCAt                                                                                                                                      <  1:1623847/151‑1 (MQ=255)
            gCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCAGCCCGATTCGGTGCGGCGCGTCGGTATATAAGTGGAGAATGCGGCGGACAGCAt                                                                                                                                      >  1:4699794/1‑151 (MQ=255)
             ccAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATc                                                                                                                                     <  1:8871044/151‑1 (MQ=255)
                 gCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGc                                                                                                                                 >  1:10415402/1‑151 (MQ=255)
                 gCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCAGCCCGAGTCGGGGCGGCGCGACGGTATCTACCTGGAGAAGGCGGCGGACAGCATCCAGc                                                                                                                                 >  2:538533/1‑151 (MQ=255)
                  cTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCt                                                                                                                                >  2:2350024/1‑151 (MQ=255)
                     cGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGAt                                                                                                                             <  1:6042202/151‑1 (MQ=255)
                     cGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGAt                                                                                                                             <  1:521549/151‑1 (MQ=255)
                      gCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATc                                                                                                                            >  2:10018608/1‑151 (MQ=255)
                       ctgaccctgaccTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCg                                                                                                                           <  2:9102913/151‑1 (MQ=255)
                          accctgaccTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTTCGTGGAGAAGGCGGCGGACAGCATCCAGCTGGTCGTCa                                                                                                                        >  1:3916253/1‑151 (MQ=255)
                             ctgaccTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCGGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATAGTGAccc                                                                                                                     >  1:351986/1‑151 (MQ=255)
                             ctgaccTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGGCGCGCCTGCCCGAGTCGGTGCGGCGCGCCGGTATCTACGTGGAGAAGGCGGCGGACAGCAACCACCTGATCGGCAccc                                                                                                                     >  1:5742924/1‑151 (MQ=255)
                              tgaccTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCAcccg                                                                                                                    >  2:8391656/1‑150 (MQ=255)
                              tgaccTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCt                                                                                                                    <  2:10786595/151‑1 (MQ=255)
                               gaccTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTc                                                                                                                   <  2:5671469/151‑1 (MQ=255)
                                accTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCa                                                                                                                  <  1:5127303/151‑1 (MQ=255)
                                 ccTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACCTGGAGAAGGCGGCGGTCAGCATCCCGCTGATCGTCACCCTCAc                                                                                                                 >  1:10016953/1‑151 (MQ=255)
                                  cTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATACAGCTGATCGTCccccgaacc                                                                                                                >  2:3960867/1‑146 (MQ=255)
                                   ttCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCAcct                                                                                                               <  1:3638901/151‑1 (MQ=255)
                                    tCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCAcctc                                                                                                              <  1:300246/151‑1 (MQ=255)
                                       gCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCCCCCTCACctcctc                                                                                                           >  2:10222897/1‑151 (MQ=255)
                                       gCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGGATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACcacctc                                                                                                           >  1:7309165/1‑151 (MQ=255)
                                         cAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGTTCGTCACCCTCACCtcctccc                                                                                                         >  1:5610624/1‑150 (MQ=255)
                                         cAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCa                                                                                                         <  1:4642729/151‑1 (MQ=255)
                                         cAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGGGCGGCGCGACGGGATCTACGGGGGGTAGGCGGCGGACAGCATCCAGCAGATCGTCACCCTCACCTCCTCCa                                                                                                         >  2:10077846/1‑151 (MQ=255)
                                            ggCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCg                                                                                                      >  1:8076796/1‑151 (MQ=255)
                                             gCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCgg                                                                                                     <  2:4131378/151‑1 (MQ=255)
                                              cGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATATACGTGGTGAAGGCGGCGGACAGCATCCAGCAGATCGTCACCCACACCGCCTCCCGCGGc                                                                                                    >  1:8449051/1‑151 (MQ=255)
                                               gTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGCGGCGGTGCGGGGCGACGGTTTCTACGTGGAGAAGGCGGCGGGCGGCATCCAGCTGATCGTCACCCTCACCACCGCCAGCGGcc                                                                                                   >  2:2710962/1‑151 (MQ=255)
                                                tGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCg                                                                                                  <  2:6637662/151‑1 (MQ=255)
                                                 gAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGc                                                                                                 <  2:9566128/151‑1 (MQ=255)
                                                   aCGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTa                                                                                               <  1:1882384/151‑1 (MQ=255)
                                                     gCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTacg                                                                                             <  1:6652987/151‑1 (MQ=255)
                                                     gCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGGGCGGCGCGACGGTATATACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCACACCTCCTCCAGCGGCCGCTacg                                                                                             >  2:2567420/1‑151 (MQ=255)
                                                       cGACCTCGCCGCGGTGGAAGTGCAGAACCTCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTacgac                                                                                           <  2:1456024/151‑1 (MQ=255)
                                                       cGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGCGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCGCCCCTCCACCAGCGGCCGCgacgac                                                                                           >  1:8591286/1‑151 (MQ=255)
                                                        gACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGCCGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCACCAGCGGCCGCGacgacg                                                                                          >  1:10928556/1‑151 (MQ=255)
                                                        gACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATAGACGTGGAGAAGGCGGCGGACAGCATACAGCTGATCGTCACCCGCACCACCTCCAGCGGCCGCTacgacg                                                                                          >  2:1937480/1‑151 (MQ=255)
                                                         aCCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGGGCGGCGCGACGGTATCTAAGTGGAGAAGGCGGCGGACAGCATCCCGCGGATCGTCACCCACACCTCCTCCAGCGGCCGCTACGACGc                                                                                         >  1:9691648/1‑151 (MQ=255)
                                                           cTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCa                                                                                       <  1:2578506/151‑1 (MQ=255)
                                                              gccgcGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGTGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCAACCAGCTGATCGTCACCCACACCTCCTCCCGCGGCCGCTACGACGCCATgg                                                                                    >  1:9484570/1‑151 (MQ=255)
                                                               ccgcGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCGGCCCGAGTCGGTGCGGCGCGACGGGATCTACGTGGAGAAGGCGGCGGACCGCATCCAGCTGAGCGTCCCCCTCACCACCGCCAGCGGCCGCTACGACGcccagga                                                                                   >  2:2846158/1‑146 (MQ=255)
                                                                cgcgGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGGCGGGGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCGCACCTCCTCCCGCGGCCGCTACGACGCCAAGGaa                                                                                  >  2:9660459/1‑151 (MQ=255)
                                                                 gcgGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCAGCCCGAGTCGGTGCGGCGCGACGGTATCTACGGGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAAc                                                                                 >  2:9203992/1‑151 (MQ=255)
                                                                   ggTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGGCGCGCCTGCCCGAGTCGGGGCGGCGCGACGGTATCAACGGGGAGAAGGCGGCGGACAGCCTCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGGCGCTTCGTCGCCATGGAACTg                                                                               >  2:4496788/1‑151 (MQ=255)
                                                                     tGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGTTCGTCACCCACACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTggg                                                                             >  1:9137601/1‑151 (MQ=255)
                                                                      ggAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGc                                                                            <  2:1180159/151‑1 (MQ=255)
                                                                         aGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCgag                                                                         <  1:7007122/151‑1 (MQ=255)
                                                                          gTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCGGCCCGAGGGGGTGCGGCGCGACGGGATCTACGGGGGGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCGCACCTCCGCCAGCGGCCGCGACGACGCCATGGAACTGGGCggga                                                                        >  1:9584970/1‑151 (MQ=255)
                                                                                     ccTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCCGCGGCCGCTACGACGCCAAGGAACTGGGCGAGATCGCCTCGTcc                                                             >  2:6377033/1‑151 (MQ=255)
                                                                                       tGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCaa                                                           <  2:8141270/151‑1 (MQ=255)
                                                                                             tcgtcgAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGtt                                                     <  1:1554480/151‑1 (MQ=255)
                                                                                                tcgAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTAACTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGct                                                  >  2:5462515/1‑150 (MQ=255)
                                                                                                 cgAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAg                                                 <  1:3373834/151‑1 (MQ=255)
                                                                                                 cgAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAg                                                 <  2:2229033/151‑1 (MQ=255)
                                                                                                 cgAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAg                                                 >  2:2516353/1‑151 (MQ=255)
                                                                                                  gAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAgg                                                <  1:2051522/151‑1 (MQ=255)
                                                                                                  gAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAgg                                                >  2:209952/1‑151 (MQ=255)
                                                                                                   aGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGgc                                               >  1:2872308/1‑151 (MQ=255)
                                                                                                    gTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGgcg                                              <  1:4956453/151‑1 (MQ=255)
                                                                                                    gTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGgcg                                              <  1:9900348/151‑1 (MQ=255)
                                                                                                    gTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGgcg                                              <  2:685379/151‑1 (MQ=255)
                                                                                                    gTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGgcg                                              <  2:6302592/151‑1 (MQ=255)
                                                                                                      cgcgCCTGCCCGAGTCGGTGCTGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCTCCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCATCGTCCTACGTGTTGCAGGCGCt                                            <  2:8406325/151‑1 (MQ=255)
                                                                                                      cgcgCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCt                                            >  1:793520/1‑151 (MQ=255)
                                                                                                       gcgcCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTg                                           <  2:7336408/151‑1 (MQ=255)
                                                                                                        cgcCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTgc                                          <  2:8881970/151‑1 (MQ=255)
                                                                                                             gCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCgg                                     >  1:2283830/1‑151 (MQ=255)
                                                                                                               ccGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGt                                   <  2:7875681/151‑1 (MQ=255)
                                                                                                               ccGAGTCGGTGCGGCGCGACGGTATCGACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGt                                   <  2:10605737/151‑1 (MQ=255)
                                                                                                                 gAGTCGGTGCGGCGCGACGGTATCTACGTGGAGACGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTgg                                 <  1:10266992/151‑1 (MQ=255)
                                                                                                                  aGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGa                                >  2:5136727/1‑151 (MQ=255)
                                                                                                                   gTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAg                               <  2:7484965/151‑1 (MQ=255)
                                                                                                                     cGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAggg                             <  1:1387375/151‑1 (MQ=255)
                                                                                                                       gTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCg                           <  2:1824719/151‑1 (MQ=255)
                                                                                                                          cggcgCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTgg                        >  2:3547500/1‑151 (MQ=255)
                                                                                                                               cgACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGCTCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAg                   <  2:1680090/151‑1 (MQ=255)
                                                                                                                               cgACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAg                   >  2:7239894/1‑151 (MQ=255)
                                                                                                                                gACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAgg                  <  2:4341605/151‑1 (MQ=255)
                                                                                                                                gACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAgg                  >  1:6263804/1‑151 (MQ=255)
                                                                                                                                 aCGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGt                 <  1:10544230/151‑1 (MQ=255)
                                                                                                                                   ggTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTcg               <  1:4188496/151‑2 (MQ=255)
                                                                                                                                     tATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCGAg             >  2:10988951/1‑151 (MQ=255)
                                                                                                                                       tCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCGAGAc           <  1:8359090/151‑1 (MQ=255)
                                                                                                                                          aCGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCGAGACCTg        <  2:10324467/151‑1 (MQ=255)
                                                                                                                                          aCGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCGAGACCTg        <  1:7474258/151‑1 (MQ=255)
                                                                                                                                          aCGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCGAGACCTg        <  1:3167440/151‑1 (MQ=255)
                                                                                                                                           cGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGGGGAGGGCGTGGGCAAGGTCGAGACCTgg       >  2:3873887/1‑151 (MQ=255)
                                                                                                                                             tGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCGAGACCTgggg     >  1:10144047/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CCAGCAGCACCGGCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAAGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCCAGACCTGGGGCGC  >  NC_008463/3423133‑3423427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑