Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,443,292 C→G P459A (CCC→GCC)  PA14_RS15680 → GntP family permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,443,2920CG100.0% 1564.0 / NA 406P459A (CCC→GCC) PA14_RS15680GntP family permease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (226/180);  total (226/180)

Only 100 of 408 total aligned reads displayed.

CGGCGGGATGGATACCCTGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACCCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAGGCCGGCGCCGACCTG  >  NC_008463/3443144‑3443438
                                                                                                                                                    |                                                                                                                                                  
   cggGATGGAGACCCTGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCAcc                                                                                                                                               <  2:5660501/151‑1 (MQ=255)
      gatggatACCCTGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGc                                                                                                                                            >  2:4046977/1‑151 (MQ=255)
        tggatACCCTGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCt                                                                                                                                          <  2:5042207/151‑1 (MQ=255)
         ggatACCCTGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTg                                                                                                                                         <  1:6339198/151‑1 (MQ=255)
         ggatACCCTGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTg                                                                                                                                         >  2:4137812/1‑151 (MQ=255)
            tACCCTGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGTACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTc                                                                                                                                      >  1:26434/1‑151 (MQ=255)
             aCCCTGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCt                                                                                                                                     >  1:6031476/1‑151 (MQ=255)
             aCCCTGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCt                                                                                                                                     <  1:1960220/151‑1 (MQ=255)
             aCCCTGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATAGGGAGGCGAACAAGGAGCATATCTGGATTGAACTGAACAAGACCCTGCAGGTGTTCTTAGACATCGGTCTGTTCTGCGCGACCGGCCAGGTCt                                                                                                                                     >  1:742941/1‑151 (MQ=255)
              cccTGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTg                                                                                                                                    <  1:4069379/151‑1 (MQ=255)
                 tGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATc                                                                                                                                 <  1:4743700/151‑1 (MQ=255)
                  gCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATcc                                                                                                                                <  1:9404979/151‑1 (MQ=255)
                      cacaACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGccc                                                                                                                            >  1:3427858/1‑151 (MQ=255)
                       acaACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCt                                                                                                                           >  2:237272/1‑151 (MQ=255)
                         aaCGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCttt                                                                                                                         >  1:8624512/1‑151 (MQ=255)
                          aCGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCtttt                                                                                                                        <  2:10053043/151‑1 (MQ=255)
                             gcgcGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTc                                                                                                                     >  2:6807625/1‑151 (MQ=255)
                             gcgcGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTc                                                                                                                     <  1:1042076/151‑1 (MQ=255)
                              cgcgGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCa                                                                                                                    <  1:9156854/151‑1 (MQ=255)
                              cgcgGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCa                                                                                                                    <  1:3114333/151‑1 (MQ=255)
                                 ggTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATcc                                                                                                                 <  2:10489803/151‑1 (MQ=255)
                                 ggTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATcc                                                                                                                 <  1:1252604/151‑1 (MQ=255)
                                   tGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTg                                                                                                               >  1:5844404/1‑151 (MQ=255)
                                    gATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGc                                                                                                              <  1:70944/151‑1 (MQ=255)
                                     aTCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCg                                                                                                             <  1:374652/151‑1 (MQ=255)
                                      tCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGTTCCGCCCTTTTTTCATCCTGCgg                                                                                                            >  2:7737347/1‑151 (MQ=255)
                                       cACCCTCCTGGCGGTCACCGGCCTGACCCATCGTGTGGCGTACTAGGACATTATATGATTTACCGTGTTCATGTCCGTGGGGGGGTAAATGGTCAGCGCTCTGATGTACGCAAGCGGCGTGTTGGGCTCCGACCTGTTGTCGTCCTGCggg                                                                                                           >  2:10280510/1‑151 (MQ=255)
                                       cACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGTCTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCggg                                                                                                           <  2:11105018/151‑1 (MQ=255)
                                       cACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCggg                                                                                                           >  1:10810967/1‑151 (MQ=255)
                                        aCCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGga                                                                                                          >  2:2177850/1‑151 (MQ=255)
                                        aCCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGga                                                                                                          >  2:2982376/1‑151 (MQ=255)
                                          cctcctGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGTACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGgaga                                                                                                        <  2:3231122/151‑1 (MQ=255)
                                          cctcctGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGgaga                                                                                                        <  2:9750144/151‑1 (MQ=255)
                                              ctGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACa                                                                                                    >  1:7784528/1‑151 (MQ=255)
                                               tGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAg                                                                                                   <  1:8043347/151‑1 (MQ=255)
                                                 gCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCa                                                                                                 <  1:753925/151‑1 (MQ=255)
                                                   ggTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATg                                                                                               >  1:9594737/1‑151 (MQ=255)
                                                     tCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGAc                                                                                             <  2:1052101/151‑1 (MQ=255)
                                                         cGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTg                                                                                         >  1:42180/1‑151 (MQ=255)
                                                          ggCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGa                                                                                        >  1:6473283/1‑151 (MQ=255)
                                                            ccTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGaaa                                                                                      >  2:8844380/1‑151 (MQ=255)
                                                              tGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAgg                                                                                    >  1:8353293/1‑151 (MQ=255)
                                                               gACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGc                                                                                   >  1:4840807/1‑151 (MQ=255)
                                                                      cGGGAGGCGTACTAGGACATTTTCGTCATTACGCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCg                                                                            <  2:3763916/151‑1 (MQ=255)
                                                                       gggAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGc                                                                           >  1:6751935/1‑151 (MQ=255)
                                                                       gggAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGc                                                                           <  2:1535937/151‑1 (MQ=255)
                                                                       gggAGGCGAACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGGTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCTTCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGc                                                                           >  2:2726343/1‑151 (MQ=255)
                                                                         gAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGccc                                                                         <  2:8555365/151‑1 (MQ=255)
                                                                         gAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGccc                                                                         >  1:2705801/1‑151 (MQ=255)
                                                                           ggCGTAAAAGTAAATTTTCGGAATTACCCTGATAAAGACCCTGGCGGTTTTTTTCTTAATCGGACTGTTCTACGCCACCGGCCTGTTATTATCCGCCATTTTTTCATCCTGCGGGATAAACAGAATGACCCTGAAAGGCAAGACCGCCCTg                                                                       <  2:9591917/151‑1 (MQ=255)
                                                                               tACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCa                                                                   >  2:7201891/1‑151 (MQ=255)
                                                                               tACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCa                                                                   >  1:7475885/1‑151 (MQ=255)
                                                                                 cAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCAcc                                                                 <  1:2048698/151‑1 (MQ=255)
                                                                                  aaGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCg                                                                >  1:3904672/1‑151 (MQ=255)
                                                                                    ggACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGt                                                              >  1:2214365/1‑151 (MQ=255)
                                                                                    ggACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGt                                                              >  1:8481190/1‑151 (MQ=255)
                                                                                       cATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTcc                                                           <  2:5340627/151‑1 (MQ=255)
                                                                                         ttttCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTccac                                                         >  1:2477994/1‑151 (MQ=255)
                                                                                         ttttCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTccac                                                         <  1:5665965/151‑1 (MQ=255)
                                                                                          tttCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTccacc                                                        >  1:5062135/1‑151 (MQ=255)
                                                                                           ttCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTccacca                                                       <  1:8930068/151‑1 (MQ=255)
                                                                                            tCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTGCCACCAg                                                      >  1:10229560/1‑151 (MQ=255)
                                                                                             cGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTATTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTccaccaac                                                     >  2:3668746/1‑149 (MQ=255)
                                                                                                cATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGc                                                  >  1:181435/1‑151 (MQ=255)
                                                                                                cATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGc                                                  <  2:7823424/151‑1 (MQ=255)
                                                                                                   tACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATc                                               <  2:7029292/151‑1 (MQ=255)
                                                                                                      ccTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGc                                            >  2:8390303/1‑151 (MQ=255)
                                                                                                        tGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCt                                          <  1:8132427/151‑1 (MQ=255)
                                                                                                        tGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCt                                          <  2:4691784/151‑1 (MQ=255)
                                                                                                         gATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTg                                         >  2:4896979/1‑151 (MQ=255)
                                                                                                          aTCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTgg                                        <  1:4539102/151‑1 (MQ=255)
                                                                                                           tCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTggg                                       >  2:3122408/1‑151 (MQ=255)
                                                                                                             aaGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCa                                     >  2:8269196/1‑151 (MQ=255)
                                                                                                              aGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCGCTGGTCACCGGTTCCACCCGCGGCAGCGGCCTGGGCag                                    >  2:10915541/1‑150 (MQ=255)
                                                                                                              aGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCAACGGCCTGGGCAt                                    >  2:7481540/1‑151 (MQ=255)
                                                                                                               gACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATc                                   <  1:8208943/151‑1 (MQ=255)
                                                                                                                aCCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCg                                  >  2:9126215/1‑151 (MQ=255)
                                                                                                                  ccTGGCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGcc                                >  1:8725681/1‑151 (MQ=255)
                                                                                                                     ggCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTg                             >  1:9962846/1‑151 (MQ=255)
                                                                                                                     ggCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTg                             <  1:109333/151‑1 (MQ=255)
                                                                                                                      gCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGa                            >  2:4530126/1‑151 (MQ=255)
                                                                                                                      gCGGTGTTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGa                            >  1:3073757/1‑151 (MQ=255)
                                                                                                                        ggtggtCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGc                          >  1:5245333/1‑151 (MQ=255)
                                                                                                                        ggTGTTCATCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTATTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGc                          >  2:3037798/1‑151 (MQ=255)
                                                                                                                           gtTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTg                       >  1:10059678/1‑151 (MQ=255)
                                                                                                                           gtTCTTCGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTg                       <  1:1731188/151‑1 (MQ=255)
                                                                                                                            ttcttcGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTgg                      <  2:1993795/151‑1 (MQ=255)
                                                                                                                              cttcGACATCGGACTGTTCTACGCCACAGGCCAGGTCTGATCCGCCCTTTTTTCATACTGCGGGAGAAAAAGCATGACCCTGAAATGCAAGACCGCCATGGTCACCGGTTCCACCAGCGTCATCGGCCTGGGCATCGCCCTGAGCCTGGcc                    >  2:4459004/1‑151 (MQ=255)
                                                                                                                               ttcGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCg                   >  1:4389289/1‑151 (MQ=255)
                                                                                                                                 cGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAg                 <  1:1439659/151‑1 (MQ=255)
                                                                                                                                 cGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAg                 <  1:6118033/151‑1 (MQ=255)
                                                                                                                                 cGTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAg                 <  1:3221373/151‑1 (MQ=255)
                                                                                                                                  gTCATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAgg                <  2:7370107/151‑1 (MQ=255)
                                                                                                                                    cATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAGGcc              >  1:5570372/1‑151 (MQ=255)
                                                                                                                                    cATCGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAGGcc              <  2:1177295/151‑1 (MQ=255)
                                                                                                                                       cGGACTGTTCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAGGCCGgc           <  2:3361154/151‑1 (MQ=255)
                                                                                                                                              ttCTACGCCACCGGCCTGGTCTGATCCGCCCTTTGTTCATCCTGCGGGAGACACAGCATGACCCTGAAAGGCTAGTCCGCCCTGGTCACCGGTTCCACCTGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAGGCCGGCGCCGAcc    >  2:10566237/1‑151 (MQ=255)
                                                                                                                                               tCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAGGCCGGCGCCGACCt   <  2:6429019/151‑1 (MQ=255)
                                                                                                                                               tCTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAGGCCGGCGCCGACCt   <  2:8463752/151‑1 (MQ=255)
                                                                                                                                                cTACGCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAGGCCGGCGCCGACCTg  >  2:3503946/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CGGCGGGATGGATACCCTGCCGCACAACGGCGCGGTGATCACCCTCCTGGCGGTCACCGGCCTGACCCATCGGGAGGCGTACAAGGACATTTTCGGCATTACCCTGATCAAGACCCTGGCGGTGTTCTTCGTCATCGGACTGTTCTACCCCACCGGCCTGGTCTGATCCGCCCTTTTTTCATCCTGCGGGAGAAACAGCATGACCCTGAAAGGCAAGACCGCCCTGGTCACCGGTTCCACCAGCGGCATCGGCCTGGGCATCGCCCTGAGCCTGGCCGAGGCCGGCGCCGACCTG  >  NC_008463/3443144‑3443438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑