Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,566,769 A→G pseudogene (1120/1275 nt) PA14_RS16215 → fatty acid desaturase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,566,7690AG100.0% 1698.1 / NA 433pseudogene (1120/1275 nt)PA14_RS16215fatty acid desaturase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (200/233);  total (200/233)

Only 100 of 435 total aligned reads displayed.

TGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCACCCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACAAGGCGCGCCCCTCAAAGTTCGGCCCGATGACCAAAACCTGGGGCATGTGGGAAAACTTCCTGATCCTGCGCAACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGG‑ATCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAACCCGCATCCCAGGCT  >  NC_008463/3566620‑3566915
                                                                                                                                                     |                                                                                                                                                   
tgtTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGagaa                                                                                                                                                   >  2:1033824/1‑151 (MQ=255)
tgtTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGagaa                                                                                                                                                   >  1:8219167/1‑151 (MQ=255)
  ttCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCTGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACt                                                                                                                                                 >  1:9216354/1‑151 (MQ=255)
  ttCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACt                                                                                                                                                 >  1:6362007/1‑151 (MQ=255)
     tcCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGACCTTcc                                                                                                                                              >  2:9713486/1‑151 (MQ=255)
     tcCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTcc                                                                                                                                              <  2:8412209/151‑1 (MQ=255)
      ccTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCt                                                                                                                                             >  2:3412549/1‑151 (MQ=255)
      ccTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCt                                                                                                                                             <  2:5862035/151‑1 (MQ=255)
      ccTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCt                                                                                                                                             <  1:8466418/151‑1 (MQ=255)
        ttCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGa                                                                                                                                           >  1:2285405/1‑151 (MQ=255)
        ttCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGa                                                                                                                                           >  1:2838341/1‑151 (MQ=255)
        gtCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGa                                                                                                                                           <  2:7853226/150‑1 (MQ=255)
         tCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGAt                                                                                                                                          >  1:1336666/1‑151 (MQ=255)
              cAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTgc                                                                                                                                     <  1:7299346/151‑1 (MQ=255)
               aGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTgcg                                                                                                                                    >  1:10003156/1‑151 (MQ=255)
                ggACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTgcgc                                                                                                                                   <  2:5172341/151‑1 (MQ=255)
                   ccTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGac                                                                                                                                <  2:3921103/151‑1 (MQ=255)
                    cTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGacc                                                                                                                               >  1:8744787/1‑151 (MQ=255)
                      gCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGaccac                                                                                                                             >  2:4918503/1‑151 (MQ=255)
                      gCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGaccac                                                                                                                             <  1:6985260/151‑1 (MQ=255)
                      gCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGaccac                                                                                                                             <  1:7564589/151‑1 (MQ=255)
                         cAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACctc                                                                                                                          <  1:8311887/151‑1 (MQ=255)
                          aGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCGCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGAGCCTGCGCGACCACctct                                                                                                                         >  2:5517187/1‑151 (MQ=255)
                          aGCCACGAATTCCACCA‑CCGCAAGCCGGGTGTGAACTTCTGGCGCATCGCGCCGGATCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACctct                                                                                                                         >  2:4541123/1‑151 (MQ=255)
                            ccACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGc                                                                                                                       <  2:8026114/151‑1 (MQ=255)
                             cACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGcc                                                                                                                      <  2:2818487/151‑1 (MQ=255)
                             cACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGcc                                                                                                                      <  1:3668553/151‑1 (MQ=255)
                              aCGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCcg                                                                                                                     >  2:1729871/1‑151 (MQ=255)
                                gACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCcgcg                                                                                                                   >  1:6729179/1‑151 (MQ=255)
                                 aCTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCgg                                                                                                                  <  1:10091911/151‑1 (MQ=255)
                                   ttCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGcc                                                                                                                >  1:8636821/1‑151 (MQ=255)
                                   ttCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGcc                                                                                                                >  1:1848946/1‑151 (MQ=255)
                                      cacca‑cCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGt                                                                                                             <  2:5067446/151‑1 (MQ=255)
                                         ccaccGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCg                                                                                                           <  2:10187721/148‑1 (MQ=255)
                                           accGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGa                                                                                                         <  2:8521764/150‑1 (MQ=255)
                                             cGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGAcc                                                                                                       >  2:726091/1‑151 (MQ=255)
                                             cGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGAcc                                                                                                       <  2:9434008/151‑1 (MQ=255)
                                                aaGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGt                                                                                                    >  2:9779426/1‑151 (MQ=255)
                                                 aGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGtt                                                                                                   >  2:85629/1‑151 (MQ=255)
                                                  gCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTc                                                                                                  >  1:11035618/1‑151 (MQ=255)
                                                   ccGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCg                                                                                                 >  1:10295281/1‑151 (MQ=255)
                                                    cGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCgg                                                                                                >  2:7588768/1‑151 (MQ=255)
                                                    cGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCgg                                                                                                >  1:3255737/1‑151 (MQ=255)
                                                    cGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCgg                                                                                                >  2:3473945/1‑151 (MQ=255)
                                                     gggggTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGG‑a                                                                                              <  2:1537224/151‑1 (MQ=255)
                                                       gggTGACCTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGG‑aat                                                                                            >  1:4447577/1‑149 (MQ=255)
                                                        ggTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACGTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGG‑aatc                                                                                           <  2:10624736/151‑4 (MQ=255)
                                                           gAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAAtcgtc                                                                                         <  2:6978413/151‑1 (MQ=255)
                                                                tCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTg                                                                                    <  1:3895132/151‑1 (MQ=255)
                                                                tCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTg                                                                                    <  2:6580024/151‑1 (MQ=255)
                                                                 cTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTgg                                                                                   <  1:10178232/151‑1 (MQ=255)
                                                                  tGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGt                                                                                  >  1:10976528/1‑151 (MQ=255)
                                                                  tGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGt                                                                                  <  1:4923310/151‑1 (MQ=255)
                                                                  tGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGt                                                                                  <  1:7373644/151‑1 (MQ=255)
                                                                    gcgcATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTAc                                                                                <  2:159609/151‑1 (MQ=255)
                                                                    gcgcATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTAc                                                                                <  1:2422897/151‑1 (MQ=255)
                                                                       cATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACcgc                                                                             >  1:9405496/1‑151 (MQ=255)
                                                                       cATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACcgc                                                                             >  2:2019327/1‑151 (MQ=255)
                                                                        aTCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACcgcg                                                                            <  2:8167165/151‑1 (MQ=255)
                                                                            cgcCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCACTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGaa                                                                        <  2:4202795/151‑1 (MQ=255)
                                                                             gcCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAAc                                                                       >  2:9844552/1‑151 (MQ=255)
                                                                               cGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCa                                                                     >  1:5425518/1‑151 (MQ=255)
                                                                                ggAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCAt                                                                    >  2:8681961/1‑151 (MQ=255)
                                                                                ggAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCAt                                                                    >  2:6183276/1‑151 (MQ=255)
                                                                                 gAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATg                                                                   >  1:4801501/1‑151 (MQ=255)
                                                                                  aGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATGc                                                                  >  1:7717714/1‑151 (MQ=255)
                                                                                     gcgcgGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATGCCCg                                                               <  2:7151202/151‑1 (MQ=255)
                                                                                      cgcgGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATGCCCgg                                                              <  2:6754817/151‑1 (MQ=255)
                                                                                      cgcgGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATGCCCgg                                                              <  2:6014810/151‑1 (MQ=255)
                                                                                        cgGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATGCCCGgct                                                            <  2:3135229/151‑1 (MQ=255)
                                                                                             aacgaGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATGCCCGgctcgctc                                                       <  1:10778673/151‑1 (MQ=255)
                                                                                               cgaGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCg                                                     >  2:9241612/3‑151 (MQ=255)
                                                                                                    cgcgcCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGAc                                                <  1:7210701/151‑1 (MQ=255)
                                                                                                        ccccTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCCCCGGAGACCGAt                                            <  2:6379811/151‑1 (MQ=255)
                                                                                                         cccTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATg                                           <  1:3490032/151‑1 (MQ=255)
                                                                                                          ccTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATgc                                          <  1:276055/151‑1 (MQ=255)
                                                                                                          ccTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATgc                                          <  1:5787584/151‑1 (MQ=255)
                                                                                                            tcgaaGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATgccg                                        <  2:8331093/148‑1 (MQ=255)
                                                                                                            tcgaaGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATgccg                                        >  2:375284/4‑151 (MQ=255)
                                                                                                             cgaaGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATgccgc                                       >  1:61505/3‑151 (MQ=255)
                                                                                                                   tCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCa                                 <  1:5088242/151‑1 (MQ=255)
                                                                                                                   tCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCa                                 <  2:4622270/151‑1 (MQ=255)
                                                                                                                   tCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCa                                 >  2:10974291/1‑151 (MQ=255)
                                                                                                                         cGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCca                           >  2:661966/1‑151 (MQ=255)
                                                                                                                           aTGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCcaca                         <  2:4535190/151‑1 (MQ=255)
                                                                                                                            tGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCacac                        <  2:10225999/151‑1 (MQ=255)
                                                                                                                             gACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACacc                       >  1:6956013/1‑151 (MQ=255)
                                                                                                                                 gagaCCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACaccaacc                   <  1:5955724/148‑1 (MQ=255)
                                                                                                                                    aCCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACcaaccaac                >  1:9995850/1‑151 (MQ=255)
                                                                                                                                      cTGGGCCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAAccc              >  2:5225622/1‑151 (MQ=255)
                                                                                                                                       tGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAACCCg             >  1:2084248/1‑151 (MQ=255)
                                                                                                                                        ggggCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAACCCGc            <  1:4104776/151‑1 (MQ=255)
                                                                                                                                        ggggCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAACCCGc            <  1:9069501/151‑1 (MQ=255)
                                                                                                                                           gCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAACCCGCATc         <  1:7896394/151‑1 (MQ=255)
                                                                                                                                            cATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAACCCGCATcc        >  2:8954656/1‑151 (MQ=255)
                                                                                                                                             aTGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAACCCGCATccc       <  2:1241529/151‑1 (MQ=255)
                                                                                                                                              tgtgGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAACCCGCATCCCa      >  2:2212308/1‑151 (MQ=255)
                                                                                                                                              tgtgGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTACGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAACCCGCATCCCa      >  2:4994999/1‑151 (MQ=255)
                                                                                                                                               gtgGGAGAACTTCCTGATCCTGCTCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAACCCGCATCCCAg     <  2:2563556/151‑1 (MQ=255)
                                                                                                                                                  ggAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAATCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAACCCGCATCCCAGGCt  >  1:1479125/1‑151 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TGTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCACCCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACAAGGCGCGCCCCTCAAAGTTCGGCCCGATGACCAAAACCTGGGGCATGTGGGAAAACTTCCTGATCCTGCGCAACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGG‑ATCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAACCCGCATCCCAGGCT  >  NC_008463/3566620‑3566915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑