Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 4,707,658 G→C A165A (GCG→GCC)  PA14_RS21625 → zinc‑binding alcohol dehydrogenase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084634,707,6580GC99.8% 2214.8 / NA 573A165A (GCG→GCC) PA14_RS21625zinc‑binding alcohol dehydrogenase family protein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base C (246/326);  total (247/326)
Fisher's exact test for biased strand distribution p-value = 4.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 573 total aligned reads displayed.

CCCAGGCCGCCAGTTGCGGGGTGCCCTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCGGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCGGAGGTGATCTTCGATACG  >  NC_008463/4707510‑4707805
                                                                                                                                                    |                                                                                                                                                   
     gccgccAGTTGCGGGGTGCCCTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGa                                                                                                                                              >  1:8808320/1‑151 (MQ=255)
       cgccAGTTGCGGGGTGCCCTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATc                                                                                                                                            <  2:8023291/151‑1 (MQ=255)
          cAGTTGCGGGGTGCCCTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGgc                                                                                                                                         <  1:3337549/151‑1 (MQ=255)
             ttGCGGGGTGCCCTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGcgcg                                                                                                                                      >  1:8160873/1‑151 (MQ=255)
             ttGCGGGGTGCCCTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGcgcg                                                                                                                                      <  2:2388636/151‑1 (MQ=255)
              tGCGGGGTGCCCTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCgg                                                                                                                                     <  2:7909676/151‑1 (MQ=255)
               gCGGGGTGCCCTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGt                                                                                                                                    <  2:1348960/151‑1 (MQ=255)
                 ggggTGCCCTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTgc                                                                                                                                  >  2:9233556/1‑151 (MQ=255)
                 ggggTGCCCTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTgc                                                                                                                                  <  1:10609777/151‑1 (MQ=255)
                  gggTGCCCTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTgcg                                                                                                                                 >  1:6819552/1‑151 (MQ=255)
                   ggTGCCCTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTgcgg                                                                                                                                <  2:7106967/151‑1 (MQ=255)
                    gTGCCCTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTgcggc                                                                                                                               <  1:3517677/151‑1 (MQ=255)
                     tGCCCTACAGCACCGCCTGGGACGCCCTGCACCGCAGCCAGGTCAAGGCCGGCCCCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTgcggcg                                                                                                                              <  1:4736836/151‑1 (MQ=255)
                     tGCCCTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTgcggcg                                                                                                                              <  2:5464521/151‑1 (MQ=255)
                     tGCCCTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTgcggcg                                                                                                                              <  2:6250478/151‑1 (MQ=255)
                      gCCCTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGgcgc                                                                                                                             >  2:8533233/1‑151 (MQ=255)
                      gCCCTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGgcgc                                                                                                                             <  1:4166591/151‑1 (MQ=255)
                         cTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCgag                                                                                                                          >  1:2201770/1‑151 (MQ=255)
                         cTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCgag                                                                                                                          <  2:4273340/151‑1 (MQ=255)
                            cAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCGCCGGGTAATCGGCGGCCAGGGCGCGGGCGGCACGGCCGGGCTGGCGCTGGCCAAGGCCCGGGGGGCCGACGCGTGCGGCGCGGTGCGGCGCgaggtg                                                                                                                       >  2:7466552/1‑149 (MQ=255)
                             aGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGc                                                                                                                      <  1:5517595/151‑1 (MQ=255)
                              gCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCa                                                                                                                     >  1:7433799/1‑151 (MQ=255)
                                aCCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCGGGGGGGCCGAAGCGAGCGGCGCGGTGCTGCGCGGGGAGCAAc                                                                                                                   >  2:1884717/1‑151 (MQ=255)
                                aCCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAAc                                                                                                                   <  1:1180246/151‑1 (MQ=255)
                                 ccGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGTCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGTGCAACg                                                                                                                  <  2:1573196/151‑1 (MQ=255)
                                    ccTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCGGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCGGGGGGCCGAAGCGAGCGGCGCGGTGCGGCGCGAGGAGCACCGCCa                                                                                                               >  2:2475224/1‑151 (MQ=255)
                                      tGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAgg                                                                                                             <  1:165318/151‑1 (MQ=255)
                                         gACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGgcgc                                                                                                          <  2:2908610/151‑1 (MQ=255)
                                          aCGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCt                                                                                                         >  1:9850897/1‑151 (MQ=255)
                                           cGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTg                                                                                                        >  2:6871102/1‑151 (MQ=255)
                                           cGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTg                                                                                                        >  2:2226217/1‑151 (MQ=255)
                                              ccTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGaa                                                                                                     <  2:10243610/151‑1 (MQ=255)
                                                tGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAgc                                                                                                   <  1:2520086/151‑1 (MQ=255)
                                                tGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAgc                                                                                                   >  2:4921732/1‑151 (MQ=255)
                                                   aaCGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCa                                                                                                >  1:5280990/1‑151 (MQ=255)
                                                    aCGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAg                                                                                               >  1:6895618/1‑151 (MQ=255)
                                                      gCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCTAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAggg                                                                                             <  1:10002579/151‑1 (MQ=255)
                                                       cAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGc                                                                                            <  1:2061210/151‑1 (MQ=255)
                                                         gCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCAt                                                                                          <  1:1402793/151‑1 (MQ=255)
                                                            aGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATcgc                                                                                       <  1:5620492/151‑1 (MQ=255)
                                                            aGGTCAAGGCCGGCACCCGGCTCCGGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATcgc                                                                                       >  1:1483847/1‑151 (MQ=255)
                                                              gTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATcgccg                                                                                     <  2:1255280/151‑1 (MQ=255)
                                                                cAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCcgcg                                                                                   >  1:4045316/1‑151 (MQ=255)
                                                                cAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCAGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCcgcg                                                                                   >  1:5137041/1‑151 (MQ=255)
                                                                   ggCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCgctg                                                                                >  2:9106535/1‑151 (MQ=255)
                                                                      cGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCgctgctg                                                                             >  1:6339367/1‑151 (MQ=255)
                                                                        gCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCgctgctgct                                                                           >  2:3905363/1‑151 (MQ=255)
                                                                         cACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGctgctgctg                                                                          <  1:5029938/151‑1 (MQ=255)
                                                                           cccGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTggg                                                                        >  1:9334874/1‑151 (MQ=255)
                                                                            ccGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTgggn                                                                       <  1:7576558/151‑2 (MQ=255)
                                                                             cGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCg                                                                      <  2:10823661/151‑1 (MQ=255)
                                                                              ggCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGa                                                                     >  2:3830452/1‑151 (MQ=255)
                                                                               gCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCTCCGCGCTGCTGCTGGGCGAg                                                                    <  1:5988369/151‑1 (MQ=255)
                                                                               gCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCGGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAg                                                                    <  2:6553370/151‑1 (MQ=255)
                                                                                 tCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCCTCGCCGCGCTGCTGCTGGGCGAgcc                                                                  >  2:6398842/1‑151 (MQ=255)
                                                                                  ccTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAgccg                                                                 <  1:5736364/151‑1 (MQ=255)
                                                                                    tGGTACTCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAgccggc                                                               <  1:28591/151‑1 (MQ=255)
                                                                                      gTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAgccggccg                                                             >  2:9665773/1‑151 (MQ=255)
                                                                                      gTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAgccggccg                                                             <  2:8859296/151‑1 (MQ=255)
                                                                                           cGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTg                                                        >  1:4323771/1‑151 (MQ=255)
                                                                                            ggCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCTGGGCATCGCCGCGCTGGTGCTGGGCGAGCCGGCCGAACTgg                                                       >  1:2708498/1‑151 (MQ=255)
                                                                                            ggCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCGGGGCGAGCCGGCCGAACTgg                                                       >  1:5367382/1‑151 (MQ=255)
                                                                                               gcCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTggcgg                                                    >  2:5227976/1‑151 (MQ=255)
                                                                                                  aaCGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGcac                                                 >  1:1930068/1‑151 (MQ=255)
                                                                                                    cGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAg                                               <  1:726777/151‑1 (MQ=255)
                                                                                                     ggCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGACGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGACGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAgg                                              <  2:4733851/151‑1 (MQ=255)
                                                                                                      gcgcGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGt                                             >  1:3595009/1‑151 (MQ=255)
                                                                                                      gcgcGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGt                                             <  1:7826620/151‑1 (MQ=255)
                                                                                                      gcgcGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGt                                             <  1:9481688/151‑1 (MQ=255)
                                                                                                       cgcgGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTg                                            >  1:1070666/1‑151 (MQ=255)
                                                                                                       cgcgGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTg                                            <  1:11090729/151‑1 (MQ=255)
                                                                                                       cgcgGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTg                                            <  1:3231093/151‑1 (MQ=255)
                                                                                                       cgcgGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTg                                            >  1:8069453/1‑151 (MQ=255)
                                                                                                        gcgGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGACCTGGCGGCACAGGTgg                                           >  2:11051478/1‑151 (MQ=255)
                                                                                                        gcgGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTgg                                           <  2:3056780/151‑1 (MQ=255)
                                                                                                         cgGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGa                                          <  2:1947892/151‑1 (MQ=255)
                                                                                                            tCGGCAAGGCCGCGCTGGCGCTGGCCACGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAgg                                       <  2:8676166/151‑1 (MQ=255)
                                                                                                              ggCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGa                                     >  1:376598/1‑151 (MQ=255)
                                                                                                              ggCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGa                                     >  1:8365844/1‑151 (MQ=255)
                                                                                                               gCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGAt                                    >  1:8576762/1‑151 (MQ=255)
                                                                                                                 aaGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCt                                  >  2:341039/1‑151 (MQ=255)
                                                                                                                  aGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCtt                                 <  2:1701257/151‑1 (MQ=255)
                                                                                                                  aGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCtt                                 <  2:4233439/151‑1 (MQ=255)
                                                                                                                    gCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTcc                               <  2:7196625/151‑1 (MQ=255)
                                                                                                                        cgctggcgctggCCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCcgg                           >  2:3284414/1‑151 (MQ=255)
                                                                                                                         gctggcgctggcCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCcggc                          <  2:6476541/151‑1 (MQ=255)
                                                                                                                          ctggcgctggcCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCcggcg                         >  2:10397311/1‑151 (MQ=255)
                                                                                                                           tggcgctggcCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCcggcgg                        <  2:4376745/151‑1 (MQ=255)
                                                                                                                            ggcgctggcCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCggcggg                       >  1:10451546/1‑151 (MQ=255)
                                                                                                                              cgctggcCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCggcggggc                     >  2:10695690/1‑151 (MQ=255)
                                                                                                                                   gcCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAgg                <  2:9443988/151‑1 (MQ=255)
                                                                                                                                   gcCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAgg                >  2:8238451/1‑151 (MQ=255)
                                                                                                                                   gcCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAgg                <  2:4011546/151‑1 (MQ=255)
                                                                                                                                   gcCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAgg                <  1:6539535/151‑1 (MQ=255)
                                                                                                                                    ccAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGt               <  2:8443732/151‑1 (MQ=255)
                                                                                                                                     cAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTg              >  2:5828372/1‑151 (MQ=255)
                                                                                                                                        ggCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATc           <  1:9193210/151‑1 (MQ=255)
                                                                                                                                            cTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCg       >  2:99178/1‑151 (MQ=255)
                                                                                                                                              gggggCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGAt     <  1:10297488/151‑1 (MQ=255)
                                                                                                                                               ggggCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATa    <  2:3235323/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CCCAGGCCGCCAGTTGCGGGGTGCCCTACAGCACCGCCTGGGACGCCCTGCAACGCAGCCAGGTCAAGGCCGGCACCCGGCTCCTGGTAATCGGCGCCAACGGCGCGGTCGGCAAGGCCGCGCTGGCGCTGGCCAAGGCCCTGGGGGCGGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCGGAGGTGATCTTCGATACG  >  NC_008463/4707510‑4707805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑