Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 4,707,787 G→C A208A (GCG→GCC)  PA14_RS21625 → zinc‑binding alcohol dehydrogenase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084634,707,7870GC99.8% 2300.1 / NA 591A208A (GCG→GCC) PA14_RS21625zinc‑binding alcohol dehydrogenase family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (262/328);  minor base A (0/1);  total (262/329)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 628 total aligned reads displayed.

TGGCCAAGGCCCTGGGGGCGGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCGGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTCGCGGTGGCTCGGTG  >  NC_008463/4707639‑4707934
                                                                                                                                                    |                                                                                                                                                   
   ccAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGt                                                                                                                                                <  2:8443732/151‑1 (MQ=255)
    cAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTg                                                                                                                                               <  2:3220195/151‑1 (MQ=255)
         cccTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCtt                                                                                                                                          >  2:4250213/1‑151 (MQ=255)
         cccTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCGGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCtt                                                                                                                                          <  2:6743579/151‑1 (MQ=255)
           cTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCg                                                                                                                                        >  2:10209364/1‑151 (MQ=255)
              ggggCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATa                                                                                                                                     <  1:362002/151‑1 (MQ=255)
              ggggCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATa                                                                                                                                     <  2:4064585/151‑1 (MQ=255)
                 gccgAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGa                                                                                                                                  <  2:1386131/148‑1 (MQ=255)
                    gAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCg                                                                                                                               <  1:8337114/151‑1 (MQ=255)
                    gAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCg                                                                                                                               <  1:9628666/151‑1 (MQ=255)
                       gCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGtt                                                                                                                            <  2:6565793/151‑1 (MQ=255)
                        cGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGttt                                                                                                                           >  2:7481985/1‑151 (MQ=255)
                        cGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCAACGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGttt                                                                                                                           <  1:7757578/151‑1 (MQ=255)
                         gATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTc                                                                                                                          >  2:4324252/1‑151 (MQ=255)
                          aTCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCt                                                                                                                         >  1:7025383/1‑151 (MQ=255)
                           tCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTg                                                                                                                        <  2:3466431/151‑1 (MQ=255)
                            cGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTgg                                                                                                                       <  1:5865158/151‑1 (MQ=255)
                              gcgcGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCt                                                                                                                     <  1:2844718/151‑1 (MQ=255)
                                gcgGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCg                                                                                                                   <  1:168290/151‑1 (MQ=255)
                                 cgGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGa                                                                                                                  <  1:10508157/151‑1 (MQ=255)
                                  ggTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCgtg                                                                                                                 >  1:8678469/1‑149 (MQ=255)
                                  ggTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAg                                                                                                                 <  1:3265617/151‑1 (MQ=255)
                                  ggTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAg                                                                                                                 >  2:6398677/1‑151 (MQ=255)
                                   gTGCGGCGCGAGGAGCAACGCCAGGCGCTGGCAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAgg                                                                                                                <  2:4859411/151‑1 (MQ=255)
                                     gcggcgCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAggcg                                                                                                              <  2:1805781/151‑1 (MQ=255)
                                     gcggcgCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAggcg                                                                                                              <  1:9715748/151‑1 (MQ=255)
                                       ggcgCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAggcggc                                                                                                            <  1:7516193/151‑1 (MQ=255)
                                       ggcgCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAggcggc                                                                                                            <  1:8203436/151‑1 (MQ=255)
                                       ggcgCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCGGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAggcggc                                                                                                            >  1:8865089/1‑151 (MQ=255)
                                        gcgcGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTGCGATACGACCGGTTTCTGGCTCGAGgcggcg                                                                                                           >  2:3611741/1‑151 (MQ=255)
                                          gcgAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGt                                                                                                         >  1:5707144/1‑151 (MQ=255)
                                          gcgAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGt                                                                                                         <  2:7962623/151‑1 (MQ=255)
                                           cgAGGAGCACCGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTc                                                                                                        <  1:190824/151‑1 (MQ=255)
                                           cgAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCGTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTc                                                                                                        <  2:10376845/151‑1 (MQ=255)
                                             aggagCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTccc                                                                                                      <  2:3940769/151‑1 (MQ=255)
                                             aggagCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTccc                                                                                                      >  1:10336824/1‑151 (MQ=255)
                                              ggagCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCg                                                                                                     >  2:3856969/1‑151 (MQ=255)
                                              ggagCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCg                                                                                                     >  1:6511586/1‑151 (MQ=255)
                                               gagCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCgg                                                                                                    >  1:7232126/1‑151 (MQ=255)
                                                agCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCGCGAGGCGGCGGTCCCGgc                                                                                                   <  1:250592/151‑1 (MQ=255)
                                                 gCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGgcg                                                                                                  >  1:9341955/1‑151 (MQ=255)
                                                 gCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGgcg                                                                                                  >  2:8580327/1‑151 (MQ=255)
                                                   aaCGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCt                                                                                                >  1:2220692/1‑151 (MQ=255)
                                                       ccAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGgcc                                                                                            <  2:1938289/151‑1 (MQ=255)
                                                       ccAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGgcc                                                                                            >  2:6960607/1‑151 (MQ=255)
                                                         aGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCGTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGgccgc                                                                                          <  2:9594049/151‑1 (MQ=255)
                                                           gcgcTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCt                                                                                        <  1:2695057/151‑1 (MQ=255)
                                                             gcTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTc                                                                                      <  2:5685699/151‑1 (MQ=255)
                                                              cTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCg                                                                                     >  1:810289/1‑151 (MQ=255)
                                                               tGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTGTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCgg                                                                                    >  2:2518459/1‑151 (MQ=255)
                                                                    gcgcAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGcc                                                                               <  2:9156979/151‑1 (MQ=255)
                                                                     cgcAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCt                                                                              <  2:9307478/151‑1 (MQ=255)
                                                                      gcAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTg                                                                             <  1:4705494/151‑1 (MQ=255)
                                                                      gcAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTg                                                                             <  1:1115727/151‑1 (MQ=255)
                                                                      gcAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTGCGGTCGCCTg                                                                             >  1:134851/1‑151 (MQ=255)
                                                                       cAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTgg                                                                            >  1:9975057/1‑151 (MQ=255)
                                                                          ggCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGa                                                                         <  1:946025/151‑1 (MQ=255)
                                                                                   gcgcTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATcgccg                                                                >  1:10289857/1‑151 (MQ=255)
                                                                                     gctgctgctGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCa                                                              >  1:4338741/1‑151 (MQ=255)
                                                                                        gctgctgGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCa                                                           <  1:2985695/151‑1 (MQ=255)
                                                                                          tgctgGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGc                                                         <  2:1982367/151‑1 (MQ=255)
                                                                                            ctgGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCg                                                       <  2:4131907/151‑1 (MQ=255)
                                                                                             tgGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGa                                                      >  1:7977858/1‑151 (MQ=255)
                                                                                              gggCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGAc                                                     >  2:2719806/1‑151 (MQ=255)
                                                                                                gCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACgg                                                   <  1:1475771/151‑1 (MQ=255)
                                                                                                  gAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGcc                                                 >  1:3426528/1‑151 (MQ=255)
                                                                                                     ccggccgAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCAtg                                              <  1:9117328/151‑1 (MQ=255)
                                                                                                        gccgAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTgc                                           <  2:1437819/151‑1 (MQ=255)
                                                                                                         ccgAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTgcg                                          >  1:6141845/1‑151 (MQ=255)
                                                                                                         ccgAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTgcg                                          >  2:4584204/1‑151 (MQ=255)
                                                                                                         ccgAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCGTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTgcg                                          >  2:8223762/1‑151 (MQ=255)
                                                                                                            taCTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGTGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCt                                       >  1:5321606/2‑151 (MQ=255)
                                                                                                            aaCTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCCTGTGCGCCt                                       >  2:5963527/1‑151 (MQ=255)
                                                                                                            aaCTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCt                                       >  2:10640878/1‑151 (MQ=255)
                                                                                                            aaCTGGCGGCACAGGTGGAAGGGATCGTCCCCGGCGGGGCCGAGGGGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCt                                       <  2:1573133/151‑1 (MQ=255)
                                                                                                               tGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGcc                                    <  2:225243/151‑1 (MQ=255)
                                                                                                               tGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGcc                                    >  1:5951517/1‑151 (MQ=255)
                                                                                                                ggcggcACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCg                                   <  1:8512072/151‑1 (MQ=255)
                                                                                                                     cacaGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGACCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCGGGCGATCATCGCCGCCCCAGCCGCCGGCCATGTGCGCCTGCCGGCGtt                              <  2:1211322/151‑1 (MQ=255)
                                                                                                                     cacaGGTGGAAGGGAGCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGAGCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGtt                              >  1:3883405/1‑151 (MQ=255)
                                                                                                                        aGGTGGTAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTGCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCAGGTGCGCCTGCCGGCGTTGac                           >  1:4490733/1‑150 (MQ=255)
                                                                                                                          gTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAAcc                         >  2:8669728/1‑151 (MQ=255)
                                                                                                                             gAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGt                      >  2:7726294/1‑151 (MQ=255)
                                                                                                                              aaGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGGTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCATCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTa                     <  2:5874250/151‑1 (MQ=255)
                                                                                                                                gggATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTAcc                   <  2:623990/151‑1 (MQ=255)
                                                                                                                                gggATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCGGCCGGCGTTGAACCTGTAcc                   <  2:723859/151‑1 (MQ=255)
                                                                                                                                 ggATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCg                  >  2:8913018/1‑151 (MQ=255)
                                                                                                                                  gATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGt                 <  1:5132822/151‑1 (MQ=255)
                                                                                                                                  gATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGt                 <  2:7562827/151‑1 (MQ=255)
                                                                                                                                  gATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGt                 >  1:632854/1‑151 (MQ=255)
                                                                                                                                   aTCGTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTc                <  2:5466950/151‑1 (MQ=255)
                                                                                                                                     cTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTcgg              <  2:2968216/151‑2 (MQ=255)
                                                                                                                                      ttCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTcgcg             >  2:3974937/1‑151 (MQ=255)
                                                                                                                                      ttCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTcgcg             <  1:10723099/151‑1 (MQ=255)
                                                                                                                                        ccccGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTCGCGGt           <  1:6871102/151‑1 (MQ=255)
                                                                                                                                         cccGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGGTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTCGCGGTg          <  1:10054990/151‑1 (MQ=255)
                                                                                                                                          ccGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTCGCGGTgg         <  2:9492199/151‑1 (MQ=255)
                                                                                                                                            ggcggggccgAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTCGCGGTGGCt       <  2:10836765/151‑1 (MQ=255)
                                                                                                                                             gcggggccgAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTCGCGGTGGCTc      <  2:5163067/151‑1 (MQ=255)
                                                                                                                                                 ggccgAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTCGCGGTGGCTCggtg  <  2:10999791/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TGGCCAAGGCCCTGGGGGCGGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCGGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTCGCGGTGGCTCGGTG  >  NC_008463/4707639‑4707934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑