Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 4,760,743 A→G L73P (CTT→CCT)  PA14_RS21850 ← HNH endonuclease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084634,760,7430AG100.0% 1714.7 / NA 442L73P (CTT→CCT) PA14_RS21850HNH endonuclease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (233/209);  total (233/209)

Only 100 of 448 total aligned reads displayed.

AATCTCCCGGACACCACTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAAGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGGCGCTGTATCGCCTT  >  NC_008463/4760595‑4760889
                                                                                                                                                    |                                                                                                                                                  
       cGGACACCACTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGTCTCTATGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTcagggtcagggt                                                                                                                                           <  1:9227947/151‑1 (MQ=255)
       cGGACACCACTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTcagggtcagggt                                                                                                                                           <  1:7581778/151‑1 (MQ=255)
         gACACCACTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTaa                                                                                                                                         >  2:1043245/1‑151 (MQ=255)
           caccacTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAAc                                                                                                                                       >  2:6243194/1‑151 (MQ=255)
            accacTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAAcc                                                                                                                                      <  2:7051873/151‑1 (MQ=255)
              cacTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCtt                                                                                                                                    <  2:2505117/151‑1 (MQ=255)
              cacTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCtt                                                                                                                                    <  1:6406701/151‑1 (MQ=255)
              cacTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCtt                                                                                                                                    >  2:6613268/1‑151 (MQ=255)
               acTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTg                                                                                                                                   <  2:6819385/151‑1 (MQ=255)
                cTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGc                                                                                                                                  <  1:5389429/151‑1 (MQ=255)
                 tccGCCAGGAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAATCCTTgac                                                                                                                                 <  1:3697927/149‑3 (MQ=255)
                 tACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGcc                                                                                                                                 <  2:8720000/151‑1 (MQ=255)
                 tACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGcc                                                                                                                                 >  2:7861622/1‑151 (MQ=255)
                 tACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGcc                                                                                                                                 >  2:10571621/1‑151 (MQ=255)
                  aCGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCg                                                                                                                                >  1:10881303/1‑151 (MQ=255)
                  aCGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTAGTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCg                                                                                                                                <  2:2149673/151‑1 (MQ=255)
                   cGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGa                                                                                                                               <  1:4195465/151‑1 (MQ=255)
                    gCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGAt                                                                                                                              <  1:890983/151‑1 (MQ=255)
                     ccAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATa                                                                                                                             >  2:11011702/1‑151 (MQ=255)
                     ccAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATa                                                                                                                             <  2:11110272/151‑1 (MQ=255)
                     ccAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATa                                                                                                                             <  2:5991594/151‑1 (MQ=255)
                          agtTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACgcgc                                                                                                                        >  1:9667544/1‑151 (MQ=255)
                             tGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAAt                                                                                                                     <  1:1909178/151‑1 (MQ=255)
                              gCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAATCTCTCGATCATCGCGAAGAGAGCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATa                                                                                                                    >  2:4512341/1‑151 (MQ=255)
                                tGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATAcc                                                                                                                  >  2:5604951/1‑151 (MQ=255)
                                 ggCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCa                                                                                                                 >  2:126619/1‑151 (MQ=255)
                                 ggCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCa                                                                                                                 >  2:10815819/1‑151 (MQ=255)
                                       cGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTaa                                                                                                           <  1:5791313/151‑1 (MQ=255)
                                          tGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGc                                                                                                        >  2:1891604/1‑151 (MQ=255)
                                          tGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGc                                                                                                        >  2:3537650/1‑151 (MQ=255)
                                            gCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCgg                                                                                                      <  2:8933118/151‑1 (MQ=255)
                                                 aTGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAg                                                                                                 <  1:5109299/151‑1 (MQ=255)
                                                  tGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGc                                                                                                <  2:3772718/151‑1 (MQ=255)
                                                  tGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGc                                                                                                <  1:6995517/151‑1 (MQ=255)
                                                  tGGGCTCTAGGTAGCTAATCCCTTCTTATGCCAGGACTGCCTGCTCAAAAGGCAGAAAACTCACGATCAACGCGAAGATATCACCTTACGAGCTTTCAGGTTCAGGTTAAACCTTGACGATACTCGCAATACAAAAGTAATGCGGCTGAGc                                                                                                >  2:1649063/1‑151 (MQ=255)
                                                   gggCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCa                                                                                               >  2:8082458/1‑151 (MQ=255)
                                                   gggCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCa                                                                                               <  1:8122985/151‑1 (MQ=255)
                                                   gggCTCTAGGTAGCTATTCCCTTCTTTCGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCa                                                                                               >  2:1426006/1‑151 (MQ=255)
                                                       tctAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGa                                                                                           >  2:8328470/1‑151 (MQ=255)
                                                         tAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAgg                                                                                         <  2:5307009/151‑1 (MQ=255)
                                                         tAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAgg                                                                                         >  1:8561869/1‑151 (MQ=255)
                                                          aGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGgc                                                                                        >  1:2728235/1‑151 (MQ=255)
                                                           ggTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGgcg                                                                                       >  2:8627246/1‑151 (MQ=255)
                                                             tAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCt                                                                                     >  1:3504575/1‑151 (MQ=255)
                                                              aGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTg                                                                                    >  2:2861503/1‑151 (MQ=255)
                                                               gCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGa                                                                                   <  1:312618/151‑1 (MQ=255)
                                                                 tATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATc                                                                                 >  1:4880731/1‑151 (MQ=255)
                                                                  aTTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCa                                                                                <  1:2591688/151‑1 (MQ=255)
                                                                  aTTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCa                                                                                >  1:6245970/1‑151 (MQ=255)
                                                                      ccTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCaa                                                                            >  1:869053/1‑151 (MQ=255)
                                                                        ttcttTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAg                                                                          <  2:3206371/151‑1 (MQ=255)
                                                                         tcttTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAgg                                                                         <  1:7844646/151‑1 (MQ=255)
                                                                         tcttTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAgg                                                                         <  2:4066138/151‑1 (MQ=255)
                                                                         tcttTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAgg                                                                         >  2:4089924/1‑151 (MQ=255)
                                                                          cttTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGa                                                                        <  2:9479928/151‑1 (MQ=255)
                                                                           ttttGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGAc                                                                       <  1:10563415/151‑1 (MQ=255)
                                                                           ctttGCCAGGACTGCCTGCTCACATGGCAGACAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAACGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGAc                                                                       <  2:3473360/150‑1 (MQ=255)
                                                                            tttGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACt                                                                      <  2:2374024/151‑1 (MQ=255)
                                                                            tttGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACt                                                                      <  1:4749825/151‑1 (MQ=255)
                                                                             ttGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACtt                                                                     <  2:7431728/151‑1 (MQ=255)
                                                                             ttGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACtt                                                                     >  2:2984267/1‑151 (MQ=255)
                                                                                ccAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCACCGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATc                                                                  <  1:771720/151‑1 (MQ=255)
                                                                                   ggACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGt                                                               >  2:6401828/1‑151 (MQ=255)
                                                                                     cctgccagcGCACATCGCTGAAAACTCTCTAACACCGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCACTACCAAAGGAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCa                                                             <  2:9084724/150‑1 (MQ=255)
                                                                                      ctgcctgcTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAg                                                            <  1:2444133/151‑1 (MQ=255)
                                                                                         cctgcTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCtt                                                         <  1:1358863/151‑1 (MQ=255)
                                                                                           tgcTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGc                                                       <  2:5462320/151‑1 (MQ=255)
                                                                                           tgcTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGc                                                       >  2:8406008/1‑151 (MQ=255)
                                                                                           tgcTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGc                                                       >  1:4556695/1‑151 (MQ=255)
                                                                                           agcTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGc                                                       <  1:6305693/150‑1 (MQ=255)
                                                                                            gcTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGTACTTATCTGTCAGCTTGcc                                                      >  2:9780668/1‑151 (MQ=255)
                                                                                             cTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGccc                                                     <  1:3892465/151‑1 (MQ=255)
                                                                                              tCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCt                                                    >  2:5624683/1‑151 (MQ=255)
                                                                                              tCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCt                                                    <  2:1561948/151‑1 (MQ=255)
                                                                                                aaaTGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTTAGCTTGCCCTTg                                                  <  1:930977/151‑1 (MQ=255)
                                                                                                aaaTGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTg                                                  <  1:8430247/151‑1 (MQ=255)
                                                                                                aaaTGGCTGAAAACGCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTg                                                  >  2:1741546/1‑151 (MQ=255)
                                                                                                       tGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTaaa                                           >  1:7243472/1‑151 (MQ=255)
                                                                                                        gAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAac                                          <  1:6479063/151‑1 (MQ=255)
                                                                                                           aaCTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAaccac                                       >  2:7254043/1‑151 (MQ=255)
                                                                                                           aaCTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAaccac                                       >  2:6492556/1‑151 (MQ=255)
                                                                                                             ctctCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCt                                     >  2:5081970/1‑151 (MQ=255)
                                                                                                              tctcGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTg                                    >  2:6912599/1‑151 (MQ=255)
                                                                                                                tcGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCa                                  <  1:10526390/151‑1 (MQ=255)
                                                                                                                  gATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCAcc                                >  1:10128555/1‑151 (MQ=255)
                                                                                                                    tCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCAcccc                              <  1:5982736/151‑1 (MQ=255)
                                                                                                                      aaCGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTg                            >  1:2192848/1‑151 (MQ=255)
                                                                                                                           gAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTa                       >  1:7055879/1‑151 (MQ=255)
                                                                                                                           gAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTa                       >  2:7619973/1‑151 (MQ=255)
                                                                                                                                   cACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGgc               >  2:4827394/1‑151 (MQ=255)
                                                                                                                                   cACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGgc               >  2:10196655/1‑151 (MQ=255)
                                                                                                                                   cACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGgc               >  2:9698762/1‑151 (MQ=255)
                                                                                                                                    aCCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGgcg              >  2:4194189/1‑151 (MQ=255)
                                                                                                                                     ccTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACGGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGgcgc             >  1:7380738/1‑151 (MQ=255)
                                                                                                                                      cTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGGCGCt            >  1:6096932/1‑151 (MQ=255)
                                                                                                                                        ttCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGGCGCTGt          >  1:6704678/1‑151 (MQ=255)
                                                                                                                                         tCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGGCGCTGTa         >  2:2570540/1‑151 (MQ=255)
                                                                                                                                           gAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGGCGCTGTATc       >  1:1812451/1‑151 (MQ=255)
                                                                                                                                           gAGCGTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGGCGCTGTATc       >  1:3294762/1‑151 (MQ=255)
                                                                                                                                            aGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGGCGCTGTATCg      >  2:9543499/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
AATCTCCCGGACACCACTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAAGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGGCGCTGTATCGCCTT  >  NC_008463/4760595‑4760889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑