Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 5,530,315 A→G K229K (AAA→AAG)  PA14_RS25330 → iron ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084635,530,3150AG100.0% 1348.7 / NA 348K229K (AAA→AAG) PA14_RS25330iron ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (204/144);  total (204/144)

Only 100 of 381 total aligned reads displayed.

TCAAGGGCCAGGAGGAAGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAAAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTTCATGCTCAGCGAG  >  NC_008463/5530167‑5530462
                                                                                                                                                    |                                                                                                                                                   
tCAAGGGCCAGGAGGAAGCCGAGGATTGGCTGACCGGCGTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTgaagaa                                                                                                                                                   <  2:10037245/151‑1 (MQ=255)
 cAAGGGCCAGGAGGAAGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCAGGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGaagaag                                                                                                                                                  >  2:8648590/1‑151 (MQ=255)
   aGGGCCAGGAGGAAGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAagaagga                                                                                                                                                >  2:5363162/1‑151 (MQ=255)
    gggCCAGGAGGAAGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAagaaggaa                                                                                                                                               >  2:10307596/1‑151 (MQ=255)
       ccAGGAGGAAGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAagaaggaaaag                                                                                                                                            <  1:6151670/151‑1 (MQ=255)
        cAGGAGGAAGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAagaaggaaaagg                                                                                                                                           >  1:7679363/1‑151 (MQ=255)
        cAGGAGGAAGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAagaaggaaaagg                                                                                                                                           >  1:5482639/1‑151 (MQ=255)
            aggaAGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGa                                                                                                                                       >  1:840484/1‑151 (MQ=255)
            aggaAGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGa                                                                                                                                       <  2:8167665/151‑1 (MQ=255)
              gaAGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGt                                                                                                                                     <  1:5791813/151‑1 (MQ=255)
               aaGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGtt                                                                                                                                    <  1:4675533/151‑1 (MQ=255)
               aaGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGtt                                                                                                                                    <  1:9367726/151‑1 (MQ=255)
               aaGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGtt                                                                                                                                    >  2:9159375/1‑151 (MQ=255)
                 gCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGa                                                                                                                                  >  1:6713181/1‑151 (MQ=255)
                     aGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTc                                                                                                                              <  1:1959383/151‑1 (MQ=255)
                           ggCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCt                                                                                                                        >  2:5265486/1‑151 (MQ=255)
                           ggCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCt                                                                                                                        >  1:9600045/1‑151 (MQ=255)
                             cttaCCTGCCTTAAGGCGTACGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGc                                                                                                                      <  2:4872335/148‑1 (MQ=255)
                             cTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGc                                                                                                                      >  2:3422725/1‑151 (MQ=255)
                              tGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCa                                                                                                                     >  2:9695402/1‑151 (MQ=255)
                              tGACCGGCCAGAAGGCCTCCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCa                                                                                                                     <  2:5658744/151‑1 (MQ=255)
                                aCCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATTAAAGCCTTGGAGAACGGCGAGGTCTACATGGCTATGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCact                                                                                                                   >  1:861258/1‑151 (MQ=255)
                                  cGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCactac                                                                                                                 >  1:2754287/1‑151 (MQ=255)
                                    gCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACtt                                                                                                               <  1:7985395/151‑1 (MQ=255)
                                     ccTGAAGGCCTTCGGCTCGATCTACACCAACACCGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTc                                                                                                              <  1:8889482/151‑1 (MQ=255)
                                     ccTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTc                                                                                                              >  1:3087798/1‑151 (MQ=255)
                                     ccTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCAGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTc                                                                                                              >  2:6875196/1‑151 (MQ=255)
                                      cTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCg                                                                                                             <  1:5020178/151‑1 (MQ=255)
                                         aaGGCCTTCGGCTCGATCTACACCAACCACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCATGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCa                                                                                                          <  1:921557/151‑1 (MQ=255)
                                            gCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAAcc                                                                                                       >  2:7467813/1‑151 (MQ=255)
                                              cTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAg                                                                                                     >  2:7317309/1‑151 (MQ=255)
                                              cTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAg                                                                                                     >  2:889566/1‑151 (MQ=255)
                                                tctgcgagATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGa                                                                                                   <  2:3083685/144‑1 (MQ=255)
                                                 cGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGAc                                                                                                  <  1:5155647/151‑1 (MQ=255)
                                                   gCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGAccc                                                                                                >  2:7594074/1‑151 (MQ=255)
                                                    cTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCg                                                                                               >  1:3253101/1‑151 (MQ=255)
                                                    cTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCg                                                                                               <  2:9358773/151‑1 (MQ=255)
                                                     tCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCgg                                                                                              <  1:6854191/151‑1 (MQ=255)
                                                     tCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCgg                                                                                              <  2:3931478/151‑1 (MQ=255)
                                                     tCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCgg                                                                                              <  2:6538014/151‑1 (MQ=255)
                                                      cGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCggg                                                                                             >  2:1556937/1‑151 (MQ=255)
                                                      cGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCggg                                                                                             >  1:9813426/1‑151 (MQ=255)
                                                       gATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGgc                                                                                            >  2:2648319/1‑151 (MQ=255)
                                                       gATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGgc                                                                                            <  1:2465419/151‑1 (MQ=255)
                                                         tCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGgcgc                                                                                          >  1:1406706/1‑151 (MQ=255)
                                                          cTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGgcgcg                                                                                         >  2:3240969/1‑151 (MQ=255)
                                                               ccAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGt                                                                                    >  2:4745811/1‑151 (MQ=255)
                                                               ccAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGt                                                                                    <  2:5091706/151‑1 (MQ=255)
                                                                 aacaacGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCa                                                                                  >  2:3998215/1‑151 (MQ=255)
                                                                    aacGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCg                                                                               <  1:10020834/151‑1 (MQ=255)
                                                                     acGTCACCGCCATGAAAGCAGTGGAGAACGTCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGt                                                                              <  2:4706099/151‑1 (MQ=255)
                                                                         cACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTcc                                                                          >  2:7634264/1‑151 (MQ=255)
                                                                         cACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTcc                                                                          >  2:8378594/1‑151 (MQ=255)
                                                                         cACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGACTcc                                                                          >  2:1953226/1‑151 (MQ=255)
                                                                             gCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGgcg                                                                      >  2:6698545/1‑151 (MQ=255)
                                                                               cATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGcgcc                                                                    <  1:4954440/151‑1 (MQ=255)
                                                                                   aaaGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTCCACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAg                                                                >  1:8288666/1‑151 (MQ=255)
                                                                                    aaGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGt                                                               <  2:4204601/151‑1 (MQ=255)
                                                                                     aGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTg                                                              >  1:5963827/1‑151 (MQ=255)
                                                                                      gCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGc                                                             >  2:2351522/1‑151 (MQ=255)
                                                                                         gTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCa                                                          <  2:6971641/151‑1 (MQ=255)
                                                                                          tGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCaa                                                         <  2:6000112/151‑1 (MQ=255)
                                                                                            gagaACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGt                                                       >  1:641888/1‑151 (MQ=255)
                                                                                             agaACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTc                                                      >  1:4117467/1‑151 (MQ=255)
                                                                                              gaACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTcc                                                     <  1:1457515/151‑1 (MQ=255)
                                                                                              gaACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTcc                                                     <  1:6986712/151‑1 (MQ=255)
                                                                                              gaACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTcc                                                     >  1:7183745/1‑151 (MQ=255)
                                                                                               aaCGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCa                                                    >  2:1279393/1‑151 (MQ=255)
                                                                                                   gCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCagcaa                                                >  1:10527396/1‑151 (MQ=255)
                                                                                                     gAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCagcaagc                                              >  1:4226975/1‑151 (MQ=255)
                                                                                                               tGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGa                                    <  2:10814731/151‑1 (MQ=255)
                                                                                                                 gcgcTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAg                                  <  2:9017142/151‑1 (MQ=255)
                                                                                                                 gcgcTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAg                                  >  1:10899152/1‑151 (MQ=255)
                                                                                                                 gcgcTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAg                                  <  1:9124700/151‑1 (MQ=255)
                                                                                                                 gcgcTGAGCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGacg                                  >  2:146169/1‑149 (MQ=255)
                                                                                                                   gcTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGcc                                <  1:10249162/151‑1 (MQ=255)
                                                                                                                     tGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCca                              <  2:3568600/151‑1 (MQ=255)
                                                                                                                      gATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCcag                             <  1:2673155/151‑1 (MQ=255)
                                                                                                                       aTCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCcagc                            >  1:3841979/1‑151 (MQ=255)
                                                                                                                           acaacTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGtt                        <  1:4909248/151‑1 (MQ=255)
                                                                                                                            caacTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTtc                       <  2:9811630/151‑1 (MQ=255)
                                                                                                                            caacTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTtc                       >  1:7502903/1‑151 (MQ=255)
                                                                                                                             aacTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTtcg                      >  1:6359689/1‑151 (MQ=255)
                                                                                                                              actactactGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTtcgt                     >  1:5423568/1‑151 (MQ=255)
                                                                                                                              actactactGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTtcgt                     >  1:3409239/1‑151 (MQ=255)
                                                                                                                                tactactGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCTGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTtcgtcg                   >  1:2142739/1‑151 (MQ=255)
                                                                                                                                tactactGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCCGGGACGCCCAGCAGTtcgtcg                   >  2:6212912/1‑151 (MQ=255)
                                                                                                                                tactactGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTtcgtcg                   <  1:10697856/151‑1 (MQ=255)
                                                                                                                                  ctactGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGcc                 >  1:1800381/1‑151 (MQ=255)
                                                                                                                                       ggTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTTCAt            <  1:7841892/151‑1 (MQ=255)
                                                                                                                                        gTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTTCATg           >  1:5842011/1‑151 (MQ=255)
                                                                                                                                         tACACCCTGTAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTTCATGc          >  1:4489176/1‑151 (MQ=255)
                                                                                                                                         tACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTTCATGc          >  1:1249144/1‑151 (MQ=255)
                                                                                                                                         tACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTTCATGc          >  1:1377073/1‑151 (MQ=255)
                                                                                                                                           cacCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTTCATGCTc        >  1:1691697/1‑151 (MQ=255)
                                                                                                                                            acCCTGAAGAAGGATAAGGGCGAGTTGAACTCGCGCCTGCACTTCGTCGGCAACCAGGACCCGGGCGCGCGGGTCACCGTCTCAGGCGCCGCAGTGCTCAAGTCCAGCAAGCTCCCCATGGATGCCCAGCAGTTCGTCGCCTTCATGCTCa       <  2:4467773/151‑1 (MQ=255)
                                                                                                                                                tGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGACTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTTCATGCTCAGCGa   >  2:368475/1‑151 (MQ=255)
                                                                                                                                                 gaagaaGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTTCATGCTCAGCGAg  >  1:1565265/1‑151 (MQ=255)
                                                                                                                                                 gaagaaGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTTCATGCTCAGCGAg  >  2:789936/1‑151 (MQ=255)
                                                                                                                                                 gaagaaGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTTCATGCTCAGCGAg  <  1:8822346/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TCAAGGGCCAGGAGGAAGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAAAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTTCATGCTCAGCGAG  >  NC_008463/5530167‑5530462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑