Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 5,968,025 C→G P426A (CCC→GCC)  phaC → class II poly(R)‑hydroxyalkanoic acid synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084635,968,0250CG99.6% 1853.0 / NA 480P426A (CCC→GCC) phaCclass II poly(R)‑hydroxyalkanoic acid synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (251/227);  minor base T (2/0);  total (253/227)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 483 total aligned reads displayed.

GAACTACTGGGTCAACAACTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCCCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGCGAGTTCATCCTCT  >  NC_008463/5967877‑5968172
                                                                                                                                                    |                                                                                                                                                   
 aaCTACTGGGTCAACAACTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGCGTTCGACGAACTGTTCAAGAGCAACCCGCTGAACAGCCCCCGcgccc                                                                                                                                                  >  2:10569460/1‑151 (MQ=255)
   ctactGGGTCAACAACTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTg                                                                                                                                                <  1:8208880/151‑1 (MQ=255)
   ctactGGGTCAACAACTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTg                                                                                                                                                <  1:10988980/151‑1 (MQ=255)
     actGGGTCAACAACTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGa                                                                                                                                              >  2:2596763/1‑151 (MQ=255)
      ctGGGTCAACAACTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAg                                                                                                                                             >  2:1116060/1‑151 (MQ=255)
         ggTCAACAACTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGtc                                                                                                                                          <  1:1743596/151‑1 (MQ=255)
         ggTCAACAACTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGtc                                                                                                                                          <  2:7263358/151‑1 (MQ=255)
            caacaaCTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTcc                                                                                                                                       >  2:10107955/1‑151 (MQ=255)
            caacaaCTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTcc                                                                                                                                       >  2:7048108/1‑151 (MQ=255)
            caacaaCTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTcc                                                                                                                                       <  2:6085955/151‑1 (MQ=255)
             aacaacTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCTCCCTGGAGGTCTCCg                                                                                                                                      >  2:1265534/1‑151 (MQ=255)
             aacaacTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCg                                                                                                                                      >  2:8947091/1‑151 (MQ=255)
              acaacTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCgg                                                                                                                                     <  1:7588933/151‑1 (MQ=255)
              acaacTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCgg                                                                                                                                     >  1:9287789/1‑151 (MQ=255)
                aacTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCa                                                                                                                                   >  1:3558825/1‑151 (MQ=255)
                 acTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGTCACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAAGTGTTCTCGAGCAACCCGCTGATCCGCCCCGGCGCGCTGGAGGTCTCCGGCag                                                                                                                                  >  2:9324171/1‑150 (MQ=21)
                  cTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACg                                                                                                                                 >  2:7994168/1‑151 (MQ=255)
                   tACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGc                                                                                                                                >  1:7916432/1‑151 (MQ=255)
                    aCCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGcc                                                                                                                               >  1:8488336/1‑151 (MQ=255)
                      cTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCa                                                                                                                             <  1:2006791/151‑1 (MQ=255)
                       tGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCAt                                                                                                                            >  2:7150384/1‑151 (MQ=255)
                        gcgcGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATc                                                                                                                           >  1:11068889/4‑151 (MQ=255)
                        gCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATc                                                                                                                           <  2:5737934/151‑1 (MQ=255)
                        gCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATc                                                                                                                           <  1:6488715/151‑1 (MQ=255)
                        gCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATc                                                                                                                           <  2:3312389/151‑1 (MQ=255)
                                aCCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGaa                                                                                                                   <  1:5238429/151‑1 (MQ=255)
                                aCCAGCCGCCGGCGTTCGACATCCTCTACGGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGaa                                                                                                                   <  2:976160/151‑1 (MQ=255)
                                  cAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGACGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGACCTGGAGGTCGCCGGCACGCCCAGCGACCTGAAGc                                                                                                                 >  2:3788032/1‑151 (MQ=255)
                                    gccgccGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAg                                                                                                               >  1:9091726/1‑151 (MQ=255)
                                     ccgccgGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAgg                                                                                                              <  2:8460953/151‑1 (MQ=255)
                                        ccgGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGa                                                                                                           <  1:10381610/151‑1 (MQ=255)
                                          ggCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACt                                                                                                         >  1:2449153/1‑151 (MQ=255)
                                           gCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACtt                                                                                                        >  2:10832578/1‑151 (MQ=255)
                                            cGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCGGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCCGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTg                                                                                                       <  2:10320656/151‑1 (MQ=255)
                                             gTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGc                                                                                                      >  1:1296800/1‑151 (MQ=255)
                                                 gACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACt                                                                                                  >  1:4010256/1‑151 (MQ=255)
                                                 gACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACt                                                                                                  >  1:497785/1‑151 (MQ=255)
                                                 gACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACt                                                                                                  >  2:4465479/1‑151 (MQ=255)
                                                 gACATCCTCCACATGACCAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGACCTGAACTAGCGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACt                                                                                                  <  2:4799690/151‑1 (MQ=255)
                                                  aCATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCACCCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTAGCGACtt                                                                                                 >  2:6617567/1‑151 (MQ=255)
                                                    aTCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCt                                                                                               >  2:10915137/1‑151 (MQ=255)
                                                     tCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTa                                                                                              <  1:5592919/151‑1 (MQ=255)
                                                      ccTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTAc                                                                                             >  1:9757000/1‑151 (MQ=255)
                                                       ctctACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACt                                                                                            >  2:2958473/1‑151 (MQ=255)
                                                            cTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTCCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTc                                                                                       >  1:6690061/1‑151 (MQ=255)
                                                               gAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGTACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGcc                                                                                    >  1:941788/1‑151 (MQ=255)
                                                                 acaacGACTCCACGCGCCTGCCCGCCGCGCTGCTCGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGTCTTCTACTGTGTCGCCgg                                                                                  <  2:1643806/151‑1 (MQ=255)
                                                                    acGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCt                                                                               >  2:1856352/1‑151 (MQ=255)
                                                                       acacCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGaa                                                                            >  2:9252/1‑151 (MQ=255)
                                                                        caccacGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAAc                                                                           <  1:7326707/151‑1 (MQ=255)
                                                                        caccacGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAAc                                                                           <  1:2497592/151‑1 (MQ=255)
                                                                          ccacGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCTTCGAACTGTTCAAGAGCTACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACATGAAGCAGGTGGCTTGCGTCGTCTACGGTGTCGCCGGTCTGAACGa                                                                         >  2:2126716/1‑151 (MQ=255)
                                                                            acGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGAcc                                                                       >  1:2992142/1‑151 (MQ=255)
                                                                             cgcgCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACca                                                                      <  1:3480959/151‑1 (MQ=255)
                                                                             cgcgCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACca                                                                      >  2:19992/1‑151 (MQ=255)
                                                                              gcgcCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACcac                                                                     <  2:3037114/151‑1 (MQ=255)
                                                                                gcCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACAt                                                                   >  2:6485675/1‑151 (MQ=255)
                                                                                gcCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACAt                                                                   >  2:7723843/1‑151 (MQ=255)
                                                                                   ggCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCAc                                                                <  1:7956694/150‑1 (MQ=255)
                                                                                     cccGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCAccc                                                              >  1:603691/1‑151 (MQ=255)
                                                                                        gccgcGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCt                                                           >  2:8244245/1‑151 (MQ=255)
                                                                                         ccgcGCTGCACGGCGAGATCGGCGAACTGGACAAGAGCAACCCGCTGACCCGCCCCGGCGCCCTGGAGGTCTCCGGCCCGCCCATCGCCCTTAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTg                                                          <  2:2275822/151‑1 (MQ=255)
                                                                                           gcgcTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTggg                                                        <  1:4798724/151‑1 (MQ=255)
                                                                                             gcTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAg                                                      >  1:10043813/1‑151 (MQ=255)
                                                                                               tGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTc                                                    <  2:7546824/151‑1 (MQ=255)
                                                                                               tGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTc                                                    >  2:5286433/1‑151 (MQ=255)
                                                                                                gCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCg                                                   >  2:3539656/1‑151 (MQ=255)
                                                                                                   cGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGc                                                >  2:632564/1‑151 (MQ=255)
                                                                                                       gAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACa                                            <  1:686975/151‑1 (MQ=255)
                                                                                                        aGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACaa                                           <  2:2667023/151‑1 (MQ=255)
                                                                                                        aGTTCGTCGAACTGTTCAAGAGCAACCAGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACaa                                           >  2:397381/1‑151 (MQ=255)
                                                                                                          ttCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGt                                         >  1:10742389/1‑151 (MQ=255)
                                                                                                          ttCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGt                                         <  1:8059876/151‑1 (MQ=255)
                                                                                                            cgtcgAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCg                                       <  2:4592064/151‑1 (MQ=255)
                                                                                                            cgtcgAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCg                                       <  1:3855941/151‑1 (MQ=255)
                                                                                                             gtcgAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCgg                                      >  1:194762/1‑151 (MQ=255)
                                                                                                             gtcgAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCgg                                      <  1:2539439/151‑1 (MQ=255)
                                                                                                                gAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCa                                   >  1:3196724/1‑151 (MQ=255)
                                                                                                                gAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCa                                   <  1:8328010/151‑1 (MQ=255)
                                                                                                                 aaCTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAg                                  <  2:194070/151‑1 (MQ=255)
                                                                                                                  aCTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAgg                                 <  1:7048108/151‑1 (MQ=255)
                                                                                                                  aCTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAgg                                 <  1:8660500/151‑1 (MQ=255)
                                                                                                                   cTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCACTGGGAGTCGTGCTACAAGTCGGCCAGGc                                >  1:4563804/1‑151 (MQ=255)
                                                                                                                    tGTTCAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCt                               >  1:1444073/1‑151 (MQ=255)
                                                                                                                        cAAGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTg                           >  2:11050510/1‑151 (MQ=255)
                                                                                                                         aaGAGCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTgg                          >  1:4235366/1‑151 (MQ=255)
                                                                                                                          agagCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTggg                         <  1:5459888/151‑1 (MQ=255)
                                                                                                                           gagCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGggc                        <  2:5665583/151‑1 (MQ=255)
                                                                                                                           gagCAACCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGggc                        <  2:143162/151‑1 (MQ=255)
                                                                                                                               aaCCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCa                    >  2:4903116/1‑151 (MQ=255)
                                                                                                                               aaCCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCa                    <  2:7897852/151‑1 (MQ=255)
                                                                                                                               aaCCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCa                    <  2:419846/151‑1 (MQ=255)
                                                                                                                               aaCCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCa                    <  1:7468565/151‑1 (MQ=255)
                                                                                                                               aaCCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCa                    >  2:10487935/1‑151 (MQ=255)
                                                                                                                                aCCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCaa                   >  2:9052114/1‑151 (MQ=255)
                                                                                                                                aCCCGCTGAACCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCaa                   <  2:9520260/151‑1 (MQ=255)
                                                                                                                                        aaCCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGCGAGt           >  2:6861380/1‑151 (MQ=255)
                                                                                                                                         aCCGCCCCGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTCCTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGCGAGtt          <  2:4536937/151‑1 (MQ=255)
                                                                                                                                              cccGGCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGCGAGTTCATcc     <  2:3627954/151‑1 (MQ=255)
                                                                                                                                                 ggCGCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGCGAGTTCATCctct  >  1:6313111/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GAACTACTGGGTCAACAACTACCTGCTCGGCAACCAGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGCGCTGCACGGCGAGTTCGTCGAACTGTTCAAGAGCAACCCGCTGAACCGCCCCGGCCCCCTGGAGGTCTCCGGCACGCCCATCGACCTGAAGCAGGTGACTTGCGACTTCTACTGTGTCGCCGGTCTGAACGACCACATCACCCCCTGGGAGTCGTGCTACAAGTCGGCCAGGCTACTGGGCGGCAAGTGCGAGTTCATCCTCT  >  NC_008463/5967877‑5968172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑