Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,198,146 Δ1 bp coding (230/1980 nt) PA14_RS14610 ← cation/acetate symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,198,1440G.99.5% 1876.9 / 0.6 381coding (232/1980 nt)PA14_RS14610cation/acetate symporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base . (186/193);  minor base T (1/1);  total (187/194)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 400 total aligned reads displayed.

CGGGCGAGGCCGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAAGGGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGATCAGCAGGAGCA  >  NC_008463/3197994‑3198290
                                                                                                                                                      |                                                                                                                                                  
cgGGCGAGGCCGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAAg                                                                                                                                                    <  2:11494867/151‑1 (MQ=255)
  ggCGAGGCCGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAAggc                                                                                                                                                  <  2:6823683/151‑2 (MQ=255)
     gAGGCCGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAAggcaca                                                                                                                                               >  2:727611/1‑147 (MQ=255)
     gAGGCCGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAAggcaca                                                                                                                                               <  1:2018490/151‑5 (MQ=255)
     gAGGCCGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAAggcaca                                                                                                                                               >  1:4017236/1‑147 (MQ=255)
      aGGCCGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACag                                                                                                                                             <  2:7956948/151‑1 (MQ=255)
       ggCCGACAGCAGGCCGGAGTGCAGCGACAACGCCCGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACaga                                                                                                                                            >  1:1768158/1‑151 (MQ=255)
        gCCGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACagaa                                                                                                                                           <  2:10836134/151‑1 (MQ=255)
              agcagGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAacac                                                                                                                                     <  1:7871868/151‑1 (MQ=255)
               gcagGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACg                                                                                                                                    >  2:5718855/1‑151 (MQ=255)
                cagGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGa                                                                                                                                   <  2:2319612/151‑1 (MQ=255)
                  ggCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAAc                                                                                                                                 >  1:7959767/1‑151 (MQ=255)
                   gCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACa                                                                                                                                <  2:1967353/151‑1 (MQ=255)
                   gCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACa                                                                                                                                <  1:924297/151‑1 (MQ=255)
                     cGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAgg                                                                                                                              <  2:4660237/151‑1 (MQ=255)
                      ggAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGa                                                                                                                             >  2:2534500/1‑151 (MQ=255)
                        aGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAac                                                                                                                           >  1:11283782/1‑151 (MQ=255)
                           gCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACg                                                                                                                        >  2:9837696/1‑151 (MQ=255)
                            cAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACgc                                                                                                                       <  1:1634356/151‑1 (MQ=255)
                            cAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACgc                                                                                                                       <  1:7009199/151‑1 (MQ=255)
                             aGCGACTACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGACTTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACgcc                                                                                                                      <  2:6631957/151‑1 (MQ=255)
                             aGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACgcc                                                                                                                      <  1:962818/151‑1 (MQ=255)
                             aGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACgcc                                                                                                                      <  2:10659138/151‑1 (MQ=255)
                               cGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGGACACAGAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACgccgg                                                                                                                    >  1:806916/1‑151 (MQ=255)
                                 aCAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACgccggcc                                                                                                                  <  2:3801722/151‑1 (MQ=255)
                                 aCAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCAGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGTTCAGGCGATCGACTTCGCCGTTGGCGCGGTACAAATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACgccggcc                                                                                                                  <  2:5008260/151‑1 (MQ=255)
                                       ccAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGAcc                                                                                                            <  2:5256334/151‑1 (MQ=255)
                                          ggggAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCg                                                                                                         <  1:8447554/151‑1 (MQ=255)
                                                 gAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCa                                                                                                  >  1:2987651/1‑151 (MQ=255)
                                                 gAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCa                                                                                                  >  2:436233/1‑151 (MQ=255)
                                                  aGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCAt                                                                                                 >  2:8467142/1‑151 (MQ=255)
                                                   gCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATg                                                                                                <  1:4778865/151‑1 (MQ=255)
                                                          cATGCGCCCCGCTCCTCTTCCAGCCCATCGACCAGAGCCTGGGTCAGGCGTTCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCTACAA‑TGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCTTGTTCGAGc                                                                                         <  2:9780806/151‑1 (MQ=255)
                                                            tGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGccc                                                                                       <  1:9500798/151‑1 (MQ=255)
                                                               gcCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTgc                                                                                    >  1:3241392/1‑151 (MQ=255)
                                                               gcCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTgc                                                                                    <  1:7626927/151‑1 (MQ=255)
                                                                ccccGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTgcg                                                                                   >  1:2545842/1‑151 (MQ=255)
                                                                ccccGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTgcg                                                                                   <  2:8885638/151‑1 (MQ=255)
                                                                 cccGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTgcgc                                                                                  >  2:8075275/1‑151 (MQ=255)
                                                                  ccGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGcc                                                                                 <  1:216699/151‑1 (MQ=255)
                                                                  ccGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGcc                                                                                 >  1:905337/1‑151 (MQ=255)
                                                                   cGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGccc                                                                                >  2:3422616/1‑150 (MQ=255)
                                                                    gCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACGGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAg                                                                               >  2:1397688/1‑151 (MQ=255)
                                                                     ctcctcTTCCAGCCCAGCGACCAGATCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTACGAGCCCTGCGCCAGa                                                                              >  1:5198229/1‑151 (MQ=255)
                                                                      tcctcTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGAc                                                                             <  1:4150508/151‑1 (MQ=255)
                                                                      tcctcTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGAc                                                                             >  2:8684178/1‑151 (MQ=255)
                                                                         tctTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGcc                                                                          <  2:4223098/151‑1 (MQ=255)
                                                                         tctTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGcc                                                                          >  2:5764944/1‑151 (MQ=255)
                                                                           ttCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTg                                                                        >  1:2703241/1‑151 (MQ=255)
                                                                              cagcccagcGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCa                                                                     >  1:91673/1‑151 (MQ=255)
                                                                               agcccagcGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACTA‑GGCTCAGAAGAACACGAACAGGACCACGCCGGCCGCGACCCCGACGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCaa                                                                    <  2:5814929/151‑1 (MQ=255)
                                                                               agcccagcGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCaa                                                                    >  2:9278571/1‑151 (MQ=255)
                                                                                gcccagcGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAAt                                                                   <  1:4921670/151‑1 (MQ=255)
                                                                                gcccagcGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAAt                                                                   <  2:11420993/151‑1 (MQ=255)
                                                                                 cccagcGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATc                                                                  <  1:1491252/151‑1 (MQ=255)
                                                                                 cccagcGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATc                                                                  <  2:3146590/151‑1 (MQ=255)
                                                                                 cccagcGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATc                                                                  <  1:4184749/151‑1 (MQ=255)
                                                                                    agcGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGc                                                               >  2:5451091/1‑151 (MQ=255)
                                                                                    agcGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGc                                                               <  2:7888903/151‑1 (MQ=255)
                                                                                     gcGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCt                                                              <  2:28306/151‑1 (MQ=255)
                                                                                       gACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCg                                                            >  2:3742474/1‑151 (MQ=255)
                                                                                       gACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCg                                                            <  2:9595740/151‑1 (MQ=255)
                                                                                           agagCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGc                                                        >  1:4541082/1‑151 (MQ=255)
                                                                                              gCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCACTCAGCTCGGGGCAGa                                                     >  1:6229625/1‑151 (MQ=255)
                                                                                               ccTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGaa                                                    >  2:10489350/1‑151 (MQ=255)
                                                                                                  gggTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGa                                                 >  1:10736090/1‑151 (MQ=255)
                                                                                                   ggTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGAt                                                <  2:10095626/151‑1 (MQ=255)
                                                                                                   ggTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGAt                                                >  1:10580006/1‑151 (MQ=255)
                                                                                                     tCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATAc                                              <  1:2479717/151‑1 (MQ=255)
                                                                                                     tCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATAc                                              >  2:7542534/1‑151 (MQ=255)
                                                                                                     tCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATAc                                              >  2:8741306/1‑151 (MQ=255)
                                                                                                         gCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAgc                                          >  1:6818397/1‑151 (MQ=255)
                                                                                                         gCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAgc                                          <  1:5368485/151‑1 (MQ=255)
                                                                                                           gATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAgcgc                                        <  2:7024070/151‑1 (MQ=255)
                                                                                                           gATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAgcgc                                        >  1:10099317/1‑151 (MQ=255)
                                                                                                                 aTTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGCGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTa                                  >  2:11203476/1‑151 (MQ=255)
                                                                                                                  ttCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAg                                 >  2:9124612/1‑151 (MQ=255)
                                                                                                                   tCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGc                                <  1:5434676/151‑1 (MQ=255)
                                                                                                                    cGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGcc                               <  1:6296614/151‑1 (MQ=255)
                                                                                                                    cGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGcc                               <  1:7318701/151‑1 (MQ=255)
                                                                                                                        gTTGGCGCGGTACACATCGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTgg                           <  2:3924503/151‑1 (MQ=255)
                                                                                                                        gTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTgg                           >  1:6502565/1‑151 (MQ=255)
                                                                                                                        gTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTgg                           >  2:897179/1‑151 (MQ=255)
                                                                                                                         ttGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGa                          <  1:2271578/151‑1 (MQ=255)
                                                                                                                          tGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGaa                         <  1:1340590/151‑1 (MQ=255)
                                                                                                                           ggCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAAc                        <  1:10955145/151‑1 (MQ=255)
                                                                                                                               cgGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTgg                    >  2:6113978/1‑151 (MQ=255)
                                                                                                                                ggTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGc                   >  2:4509584/1‑151 (MQ=255)
                                                                                                                                    cacaTAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAgg               >  2:2069092/1‑151 (MQ=255)
                                                                                                                                      caTAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGAt             >  1:11737977/1‑151 (MQ=255)
                                                                                                                                       aTAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGATc            >  2:1759027/1‑151 (MQ=255)
                                                                                                                                         agagCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGATcag          <  1:8666502/151‑1 (MQ=255)
                                                                                                                                           agCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGATcagca        >  2:11714189/1‑151 (MQ=255)
                                                                                                                                             cgcCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGATCagcagg      <  2:4824640/151‑1 (MQ=255)
                                                                                                                                             cgcCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGATCagcagg      >  1:5822245/1‑151 (MQ=255)
                                                                                                                                               ccGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGATCagcaggag    <  2:1236172/151‑1 (MQ=255)
                                                                                                                                               ccGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGATCagcaggag    <  1:10481198/151‑1 (MQ=255)
                                                                                                                                                cGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGATCagcaggagc   >  2:1662968/1‑151 (MQ=255)
                                                                                                                                                cGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGATCagcaggagc   <  1:997786/151‑1 (MQ=255)
                                                                                                                                                cGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGATCagcaggagc   >  1:564094/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                  
CGGGCGAGGCCGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAAGGGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGATCAGCAGGAGCA  >  NC_008463/3197994‑3198290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑