BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	5,931,608	(T)_6→5	intergenic (‑27/‑151)	PA14_RS27130 ← / → PA14_RS27135	LysR family transcriptional regulator/MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	5,931,603	0	T	.	100.0%	2149.0 / NA	437	intergenic (‑22/‑156)	PA14_RS27130/PA14_RS27135	LysR family transcriptional regulator/MFS transporter
Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (158/279); total (158/279)

Only 100 of 470 total aligned reads displayed.

CGATTTCCTCCTCCAGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTATTTAGGTGTTTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGCCTGCTCGCCACCGTCCCCAG  >  NC_008463/5931452‑5931749

 gATTTCCTCCTCCAGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTGtt                                                                                                                                                   >  2:4786281/1‑151 (MQ=255)

    ttCCTCCTCCAGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTGttttt                                                                                                                                                >  1:10283679/1‑151 (MQ=255)

    ttCCTCCTCCAGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTGttttt                                                                                                                                                >  1:9150896/1‑151 (MQ=255)

    ttCCTCCTCCAGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTGttttt                                                                                                                                                >  2:7020288/1‑151 (MQ=255)

    ttCCTCCTCCAGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTGtattt                                                                                                                                                <  1:4204761/151‑1 (MQ=255)

        tcctccAGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTGtttttacat                                                                                                                                            <  2:10910277/151‑5 (MQ=255)

        tcctccAGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTGtttttacat                                                                                                                                            >  1:3420408/1‑147 (MQ=255)

          ctccAGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGCTCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGTTGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACatat                                                                                                                                         <  2:8358348/151‑1 (MQ=255)

          ctccAGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACatat                                                                                                                                         <  1:5846753/151‑1 (MQ=255)

          ctccAGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACatat                                                                                                                                         <  2:10973974/151‑1 (MQ=255)

           tccAGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATc                                                                                                                                        >  1:852184/1‑151 (MQ=255)

             cAGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCGA‑TTAGGTG‑TTTTTACATATCaa                                                                                                                                      >  2:3896809/1‑151 (MQ=255)

              aGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAAt                                                                                                                                     <  1:11693400/151‑1 (MQ=255)

               gcgcCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATc                                                                                                                                    <  1:2139025/151‑1 (MQ=255)

                cgcCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCg                                                                                                                                   <  2:8245726/151‑1 (MQ=255)

                cgcCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCg                                                                                                                                   <  2:6472080/151‑1 (MQ=255)

                cgcCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCg                                                                                                                                   >  1:4637207/1‑151 (MQ=255)

                     ggATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCg                                                                                                                              <  2:10539645/151‑1 (MQ=255)

                      gATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGa                                                                                                                             <  2:1564294/151‑1 (MQ=255)

                       aTCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGAt                                                                                                                            <  2:1505461/151‑1 (MQ=255)

                        tCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCTATc                                                                                                                           <  1:951569/151‑1 (MQ=255)

                           gctgGCTCATCGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAat                                                                                                                        <  1:5396993/151‑1 (MQ=255)

                           gctgGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAat                                                                                                                        <  2:7214435/151‑1 (MQ=255)

                           gctgGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAat                                                                                                                        >  1:9252187/1‑151 (MQ=255)

                            ctgGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAata                                                                                                                       <  2:10068588/151‑1 (MQ=255)

                            ctgGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAata                                                                                                                       <  1:8120317/151‑1 (MQ=255)

                            ctgGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAAGAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCACTCGTGTCGATCAata                                                                                                                       <  2:8407041/151‑1 (MQ=255)

                             tgGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAatat                                                                                                                      >  1:1830117/1‑151 (MQ=255)

                             tgGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAatat                                                                                                                      <  1:11510140/151‑1 (MQ=255)

                             tgGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAatat                                                                                                                      <  1:6215083/151‑1 (MQ=255)

                               gCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAatatat                                                                                                                    >  2:925158/1‑151 (MQ=255)

                                cTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATAtt                                                                                                                   <  1:2789318/151‑1 (MQ=255)

                                cTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATAtt                                                                                                                   <  1:7756312/151‑1 (MQ=255)

                                  cAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTgg                                                                                                                 <  2:7365294/151‑1 (MQ=255)

                                   aaCGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGa                                                                                                                <  1:8278166/151‑1 (MQ=255)

                                       gcggcTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATc                                                                                                            <  1:6586699/151‑1 (MQ=255)

                                        cggcTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCa                                                                                                           <  2:9906697/151‑1 (MQ=255)

                                        cggcTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCa                                                                                                           <  1:157008/151‑1 (MQ=255)

                                         ggcTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCat                                                                                                          <  2:11268137/151‑1 (MQ=255)

                                         ggcTGGGACACCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCTGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCat                                                                                                          <  2:116475/151‑1 (MQ=255)

                                          gcTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCata                                                                                                         >  1:8417331/1‑151 (MQ=255)

                                             gggAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATaa                                                                                                      <  1:10168052/151‑1 (MQ=255)

                                              ggAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATaaa                                                                                                     <  2:6698174/151‑1 (MQ=255)

                                               gagaTCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAAc                                                                                                    <  2:979213/151‑1 (MQ=255)

                                                 gaTCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACct                                                                                                  <  2:9144789/151‑1 (MQ=255)

                                                   tCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACctat                                                                                                <  2:10494448/151‑1 (MQ=255)

                                                     cccAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACctatct                                                                                              <  1:7610392/151‑1 (MQ=255)

                                                     cccAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACctatct                                                                                              <  1:10746443/151‑1 (MQ=255)

                                                        aGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCtata                                                                                           <  2:8611874/151‑1 (MQ=255)

                                                         ggCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGCTCAATATATTGGAAATCATATAAACCTAGCTatat                                                                                          >  2:2830955/1‑151 (MQ=255)

                                                              tCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCttt                                                                                     <  2:4462988/151‑1 (MQ=255)

                                                              tCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCttt                                                                                     <  1:2372202/151‑1 (MQ=255)

                                                              tCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCttt                                                                                     <  1:7469619/151‑1 (MQ=255)

                                                              tCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCttt                                                                                     <  2:10416783/151‑1 (MQ=255)

                                                               cggcggCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTg                                                                                    >  2:5820099/1‑151 (MQ=255)

                                                               cggcggCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTg                                                                                    <  1:1283769/151‑1 (MQ=255)

                                                               cggcggCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTg                                                                                    <  1:7041232/151‑1 (MQ=255)

                                                               cggcggCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTg                                                                                    <  2:6334743/151‑1 (MQ=255)

                                                                ggcggcGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGc                                                                                   <  2:2056320/151‑1 (MQ=255)

                                                                 gcggcgcggcCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGcc                                                                                  <  2:8846793/151‑1 (MQ=255)

                                                                 gcggcgcggcCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGcc                                                                                  >  2:11450965/1‑151 (MQ=255)

                                                                  cggcgcggcCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCca                                                                                 <  2:1818999/151‑1 (MQ=255)

                                                                     cgcggcCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACAt                                                                              >  1:4855120/1‑151 (MQ=255)

                                                                     cgcggcCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACAt                                                                              <  1:3198616/151‑1 (MQ=255)

                                                                     cgcggcCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACAt                                                                              <  2:3710255/151‑1 (MQ=255)

                                                                        ggcCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAAc                                                                           >  1:176108/1‑151 (MQ=255)

                                                                        ggcCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAAc                                                                           >  2:7741209/1‑151 (MQ=255)

                                                                        ggcCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAAc                                                                           >  1:9670560/1‑151 (MQ=255)

                                                                        ggcCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAAc                                                                           <  2:10279596/151‑1 (MQ=255)

                                                                          ccGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCt                                                                         >  2:3304574/1‑151 (MQ=255)

                                                                           cGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTc                                                                        >  2:3484638/1‑151 (MQ=255)

                                                                            gAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTcc                                                                       <  1:6825031/151‑1 (MQ=255)

                                                                            gAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTcc                                                                       >  1:7853222/1‑151 (MQ=255)

                                                                            gAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTcc                                                                       >  1:5780245/1‑151 (MQ=255)

                                                                                       cctcGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGAccc                                                            >  2:1366106/1‑151 (MQ=255)

                                                                                       cctcGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGAccc                                                            >  2:2515405/1‑151 (MQ=255)

                                                                                         tcGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCt                                                          <  1:11316766/151‑1 (MQ=255)

                                                                                          cggcgacggcgaTGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCtt                                                         <  2:5674850/151‑1 (MQ=255)

                                                                                            gcgacggcgaTGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGt                                                       <  2:2949804/151‑1 (MQ=255)

                                                                                              gacggcgaTGAAAGAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTcc                                                     <  1:10513779/151‑1 (MQ=255)

                                                                                                     aTGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCGGAAAt                                              >  1:5138112/1‑151 (MQ=255)

                                                                                                       gAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATcc                                            >  1:3337242/1‑151 (MQ=255)

                                                                                                        aaaTAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACCACGACCCCTTGTCCCTGAAATCCa                                           >  2:4229236/1‑151 (MQ=255)

                                                                                                        aaaTAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCa                                           <  1:3854619/151‑1 (MQ=255)

                                                                                                        aaaTAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCa                                           >  1:7353894/1‑151 (MQ=255)

                                                                                                         aaTAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAg                                          <  2:9385588/151‑1 (MQ=255)

                                                                                                          aTAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑ATTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAgg                                         <  2:4946993/151‑1 (MQ=255)

                                                                                                             gcgcAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTgg                                      <  1:10948370/151‑1 (MQ=255)

                                                                                                                 aGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGcgcg                                  <  1:4988454/151‑1 (MQ=255)

                                                                                                                    tGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATCACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGcc                               >  1:6035773/1‑151 (MQ=255)

                                                                                                                      ccTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTc                             <  2:6110831/151‑1 (MQ=255)

                                                                                                                         gAGTTCCAAGCGGCCCCTCCT‑CTTAGGTG‑TTTTTACATATCACTCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCATTGCCACATAACCTCCAACCACGACCCCTTGTCCCTGAACTCCAGGTGGCGCGGCCTCGtt                          <  2:5569389/151‑1 (MQ=255)

                                                                                                                                   cGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATACACCTATCTATATCCTTTGCCACCTAACCTCCAACCACGACCCCTGGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGCCTGCTc                <  2:5459316/151‑1 (MQ=255)

                                                                                                                                    ggCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGCCTGCTCg               <  2:4349696/151‑1 (MQ=255)

                                                                                                                                        cctcctA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGCCTGCTCGCCAc           >  1:4435241/1‑151 (MQ=255)

                                                                                                                                         ctcctA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGCCTGCTCGCCAcc          <  1:8915490/151‑1 (MQ=255)

                                                                                                                                               attAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGCCTGCTCGCCACCGTccc     >  2:3206123/2‑151 (MQ=255)

                                                                                                                                               attAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGCCTGCTCGCCACCGTccc     >  1:9810325/2‑151 (MQ=255)

                                                                                                                                                ttAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGCCTGCTCGCCACCGTcccc    >  2:1955501/1‑151 (MQ=255)

                                                                                                                                                  aGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGCCTGCTCGCCACCGTCCCCag  <  2:11220294/151‑1 (MQ=255)

CGATTTCCTCCTCCAGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTATTTAGGTGTTTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGCCTGCTCGCCACCGTCCCCAG  >  NC_008463/5931452‑5931749

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`