BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	6,070,064	Δ1 bp	pseudogene (533/1086 nt)	PA14_RS27735 →	oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	6,070,063	0	T	.	99.9%	3465.5 / ‑3.8	697	pseudogene (532/1086 nt)	PA14_RS27735	oxidoreductase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base . (337/359); minor base G (1/0); total (338/359)
Fisher's exact test for biased strand distribution p-value = 4.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 718 total aligned reads displayed.

TTCCAGAACCGGCGCTTCGACAGCGACTTCCTCGCCGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGCTTCCGGGCTGTGGTTTGACCTTGGCCCGCAACCTGGTTGACCAAGGCACTGTGCCTGTTTGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCC  >  NC_008463/6069913‑6070211

ttCCAGAACCGGCGCTTCGACAGCGACTTCCTCGCCGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGCt                                                                                                                                                      >  2:9693877/1‑151 (MQ=255)

ttCCAGAACCGGCGCTTCGACAGCGACTTCCTCGCCGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGCt                                                                                                                                                      <  2:2188197/151‑1 (MQ=255)

ttCCAGAACCGGCGCTTCGACAGCGACTTCCTCGCCGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGCt                                                                                                                                                      >  2:6452088/1‑151 (MQ=255)

  ccAGAACCGGCGCTTCGACAGCGACTTCCTCGCCGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGCtcc                                                                                                                                                    <  1:7975950/151‑3 (MQ=255)

  ccAGAACCGGCGCTTCGACAGCGACGTCCTCGCCGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGCtcc                                                                                                                                                    <  2:2790800/151‑3 (MQ=255)

     gAACCGGCGCTTCGACAGCGACTTCCTCGCCGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCggg                                                                                                                                                <  2:8862509/151‑1 (MQ=255)

          ggCGCTTCGACAGCGACTTCCTCGCCGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCtgtg                                                                                                                                           <  2:1548938/151‑1 (MQ=255)

             gcTTCGACAGCGACTTCCTCGCCGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGtt                                                                                                                                        <  2:4670131/151‑1 (MQ=255)

             gcTTCGACAGCGACTTCCTCGCCGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGtt                                                                                                                                        <  2:10196418/151‑1 (MQ=255)

               ttCGACAGCGACTTCCTCGCCGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGTTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGttcg                                                                                                                                      <  1:9482198/151‑3 (MQ=255)

               ttCGACAGCGACTTCCTCGCCGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGttcg                                                                                                                                      <  2:7080395/151‑3 (MQ=255)

                 cGACAGCGACTTCCTCGCCGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGAc                                                                                                                                    <  1:8809627/151‑1 (MQ=255)

                  gACAGCGACTTCCTCGCCGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGAcc                                                                                                                                   >  2:556986/1‑151 (MQ=255)

                   aCAGCGACTTCCTCGCCGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCt                                                                                                                                  <  1:9036547/151‑1 (MQ=255)

                   aCAGCGACTTCCTCGCCGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCt                                                                                                                                  >  2:3172582/1‑151 (MQ=255)

                    cAGCGACTTCCTCGCCGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCtc                                                                                                                                 >  1:6779401/1‑150 (MQ=255)

                    cAGCGACTTCCTCGCCGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCAGCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCtc                                                                                                                                 >  2:10775285/1‑150 (MQ=255)

                      gCGACTTCCTCGCCGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCgg                                                                                                                               <  1:6318917/151‑1 (MQ=255)

                         aCTTCCTCGCCGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGccc                                                                                                                            <  1:3015937/151‑1 (MQ=255)

                             ccTCGCCGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGCac                                                                                                                        <  2:1126626/151‑2 (MQ=255)

                              cTCGCCGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGCacc                                                                                                                       <  1:10397618/151‑3 (MQ=255)

                               tCGCCGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGCacct                                                                                                                      <  2:3744672/151‑4 (MQ=255)

                               tCGCCGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGCacct                                                                                                                      <  1:9619284/151‑4 (MQ=255)

                                   cGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGCacctgttc                                                                                                                  <  1:7913318/151‑8 (MQ=255)

                                   cGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGCacctgctc                                                                                                                  >  2:11736704/1‑144 (MQ=255)

                                   cGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGCacctgctc                                                                                                                  >  1:2874414/1‑144 (MQ=255)

                                         ggCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACCag                                                                                                           <  1:1110974/151‑2 (MQ=255)

                                         ggCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACCag                                                                                                           >  2:4306947/1‑150 (MQ=255)

                                             gccCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCTCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCAc                                                                                                      <  2:7837396/151‑1 (MQ=255)

                                             gccCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCAc                                                                                                      <  2:5382588/151‑1 (MQ=255)

                                             gccCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCAc                                                                                                      >  1:7274494/1‑151 (MQ=255)

                                               ccTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACtg                                                                                                    >  1:8295728/1‑151 (MQ=255)

                                               ccTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACtg                                                                                                    <  1:76359/151‑1 (MQ=255)

                                                    aGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCt                                                                                               <  2:2333480/151‑1 (MQ=255)

                                                    aGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCt                                                                                               <  1:6421983/151‑1 (MQ=255)

                                                      gCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGt                                                                                             >  1:7149081/1‑151 (MQ=255)

                                                       ccGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGtt                                                                                            <  2:6637233/151‑1 (MQ=255)

                                                        cGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCCCTGTGCCTGttc                                                                                           >  2:10894115/1‑150 (MQ=255)

                                                        cGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGttc                                                                                           <  1:2700134/151‑2 (MQ=255)

                                                         ggCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGttcg                                                                                          <  1:7760512/151‑3 (MQ=255)

                                                            agcaTCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCa                                                                                       <  2:1396380/151‑1 (MQ=255)

                                                             gcaTCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCAt                                                                                      <  2:4933344/151‑1 (MQ=255)

                                                             gcaTCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCAt                                                                                      <  1:8577624/151‑1 (MQ=255)

                                                               aTCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATAc                                                                                    >  1:1168566/1‑151 (MQ=255)

                                                               aTCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATAc                                                                                    <  1:9917862/151‑1 (MQ=255)

                                                                tCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATAcc                                                                                   <  1:5835402/151‑1 (MQ=255)

                                                                 cGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCg                                                                                  >  1:5650181/1‑151 (MQ=255)

                                                                 cGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCg                                                                                  >  2:444844/1‑151 (MQ=255)

                                                                   gCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCa                                                                                >  2:5761453/1‑151 (MQ=255)

                                                                     ccGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGc                                                                              <  1:3217941/151‑1 (MQ=255)

                                                                     ccGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGc                                                                              >  1:4042669/1‑151 (MQ=255)

                                                                        gTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTa                                                                           <  2:10464651/151‑1 (MQ=255)

                                                                           cgcTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGc                                                                        >  2:1921286/1‑151 (MQ=255)

                                                                               aCTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACgg                                                                    <  1:3745461/151‑1 (MQ=255)

                                                                               aCTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACgg                                                                    <  1:11500371/151‑1 (MQ=255)

                                                                               aCTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACgg                                                                    >  2:3309493/1‑151 (MQ=255)

                                                                                 ttCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGac                                                                  <  1:2463455/151‑1 (MQ=255)

                                                                                  tCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGaca                                                                 <  2:8159539/151‑1 (MQ=255)

                                                                                    gAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACAcc                                                               >  2:6196464/1‑151 (MQ=255)

                                                                                     aGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCt                                                              >  2:5917185/1‑151 (MQ=255)

                                                                                      gTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTg                                                             >  2:11508599/1‑151 (MQ=255)

                                                                                          gCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGcc                                                         >  1:5015763/1‑151 (MQ=255)

                                                                                           ccATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCg                                                        >  1:9422274/1‑151 (MQ=255)

                                                                                           ccATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCg                                                        >  1:5375447/1‑151 (MQ=255)

                                                                                            cATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGGGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCgg                                                       >  2:4802994/1‑151 (MQ=255)

                                                                                                 aGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTgcg                                                  >  2:8586832/1‑151 (MQ=255)

                                                                                                      ttCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAgg                                             >  2:9233328/1‑151 (MQ=255)

                                                                                                       tccgtccgCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAggg                                            >  1:747526/1‑151 (MQ=255)

                                                                                                       tccgtccgCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAggg                                            >  1:4311608/1‑151 (MQ=255)

                                                                                                         cgtccgCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGgcg                                          >  2:6880424/1‑151 (MQ=255)

                                                                                                           tccgCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGgcgcg                                        >  1:8123143/1‑151 (MQ=255)

                                                                                                            ccgCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGgcgcgc                                       >  1:2315167/1‑151 (MQ=255)

                                                                                                             cgCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCt                                      <  1:1192818/151‑1 (MQ=255)

                                                                                                              gCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTg                                     <  2:6376967/151‑1 (MQ=255)

                                                                                                               cAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCGGCTCGACC‑AGGCACTGGGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGa                                    <  1:11047406/151‑1 (MQ=255)

                                                                                                                aGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGAc                                   <  2:7399502/151‑1 (MQ=255)

                                                                                                                aGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCGGCGCCGGCTGCGCGAGGGCGCGCTGAc                                   >  2:5199097/1‑151 (MQ=255)

                                                                                                                    ccGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACcgac                               >  1:5128624/1‑151 (MQ=255)

                                                                                                                    ccGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACcgac                               <  1:3517020/151‑1 (MQ=255)

                                                                                                                    ccGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACcgac                               <  2:5114558/151‑1 (MQ=255)

                                                                                                                     cgcgACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACcgacg                              >  2:10085268/1‑151 (MQ=255)

                                                                                                                     cgcgACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACcgacg                              <  2:9608235/151‑1 (MQ=255)

                                                                                                                       cgACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCgacgac                            >  1:6680883/1‑151 (MQ=255)

                                                                                                                        gACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACt                           >  1:7340874/1‑151 (MQ=255)

                                                                                                                          ccGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTgg                         <  2:9124885/151‑1 (MQ=255)

                                                                                                                            gCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGtt                       <  2:3988672/151‑1 (MQ=255)

                                                                                                                             cTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATCCCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTc                      <  1:1840094/151‑1 (MQ=255)

                                                                                                                             cTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTc                      >  1:1469675/1‑151 (MQ=255)

                                                                                                                              tGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCg                     >  1:10020367/1‑151 (MQ=255)

                                                                                                                               ggCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGa                    <  1:1014402/151‑1 (MQ=255)

                                                                                                                                  gcgAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGt                 <  1:10577108/151‑1 (MQ=255)

                                                                                                                                  gcgAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGt                 <  1:6524206/151‑1 (MQ=255)

                                                                                                                                      acacGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTg             <  1:6069726/151‑1 (MQ=255)

                                                                                                                                      acacGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTg             <  2:3613896/151‑1 (MQ=255)

                                                                                                                                         cGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTc          >  2:603205/1‑151 (MQ=255)

                                                                                                                                         cGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTc          <  1:4682049/151‑1 (MQ=255)

                                                                                                                                           aCCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGa        <  1:1333750/151‑1 (MQ=255)

                                                                                                                                            ccTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGAc       >  1:2029002/1‑151 (MQ=255)

                                                                                                                                            ccTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGAc       <  1:3309887/151‑1 (MQ=255)

                                                                                                                                                 ccGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATcc  <  1:4130866/151‑1 (MQ=255)

TTCCAGAACCGGCGCTTCGACAGCGACTTCCTCGCCGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGCTTCCGGGCTGTGGTTTGACCTTGGCCCGCAACCTGGTTGACCAAGGCACTGTGCCTGTTTGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATCC  >  NC_008463/6069913‑6070211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`