BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	1,280,717	(C)_6→5	intergenic (‑46/+41)	PA14_RS06005 ← / ← PA14_RS06010	DUF4345 domain‑containing protein/M23 family metallopeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	1,280,712	0	C	.	100.0%	2610.5 / NA	525	intergenic (‑41/+46)	PA14_RS06005/PA14_RS06010	DUF4345 domain‑containing protein/M23 family metallopeptidase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base . (318/207); total (318/207)

Only 100 of 576 total aligned reads displayed.

TCGCCGCTGGTGAGCAGGGAGGCGAGCGGGCCGGGGAAGAAGACGAAGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCGCCCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCG‑GGTCGACCCGCGCGTCGTTCAGGCTGACGTTCCAGTGCATGTGCGGTCCGGTGGCCCTGCCGGTGGCGC  >  NC_008463/1280562‑1280858

    cgcTGGTGAGCAGGGAGGCGAGCGGGCCGGGGAAGAAGACGAAGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCGccccc                                                                                                                                                 <  1:8123472/151‑1 (MQ=255)

     gcTGGTGAGCAGGGAGGCGAGCGGGCCGGGGAAGAAGACGACGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCGccccct                                                                                                                                                <  1:3218150/151‑2 (MQ=255)

      cTGGTGAGCAGGGAGGCGAGCGGGCCGGGGAAGAAGACGAAGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCGccccctg                                                                                                                                               >  2:3244554/1‑149 (MQ=255)

       tGGTGAGCAGGGAGGCGAGCGGGCCGGGGAAGAAGACGAAGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCTTCGACGCGccccctgt                                                                                                                                              >  1:1330546/1‑148 (MQ=255)

       tGGTGAGCAGGGAGGCGAGCGGGCCGGGGAAGAAGACGAAGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCGccccctgt                                                                                                                                              <  1:3768914/151‑4 (MQ=255)

        ggTGAGCAGGGAGGCGAGCGGGCCGGGGAAGAAGACGACGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCGccccctgtt                                                                                                                                             <  1:6653603/151‑5 (MQ=255)

         gTGAGCAGGGAGGCGAGCGGGCCGGGGAAGAAGACGAAGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTc                                                                                                                                           >  1:10164928/1‑151 (MQ=255)

         gTGAGCAGGGAGGCGAGCGGGCCGGGGAAGAAGACGAAGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTc                                                                                                                                           <  1:1168540/151‑1 (MQ=255)

            aGCAGGGAGGCGAGCGGGCCGGGGAAGAAGACGAAGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCtt                                                                                                                                        >  2:10350274/1‑151 (MQ=255)

             gCAGGGAGGCGAGCGGGCCGGGGAAGAAGACGAAGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTc                                                                                                                                       >  2:2314611/1‑151 (MQ=255)

              cAGGGAGGCGAGCGGGCCGGGGAAGAAGACGAAGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCt                                                                                                                                      <  2:11483148/151‑1 (MQ=255)

              cAGGGAGGCGAGCGGGCCGGGGAAGAAGACGAAGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCt                                                                                                                                      <  1:6446129/151‑1 (MQ=255)

               aGGGAGGCGAGCGGGCCGGGGAAGAAGACGACGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGATGCGGGTGAGTCATGCGGTGTCTCACTTGGTCATGAAAGGGGCGCATCGACGCG‑CACCGTGTTCCTTCTg                                                                                                                                     >  2:6433819/1‑151 (MQ=255)

                gggAGGCGAGCGGGCCGGGGAAGAAGACGAAGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGa                                                                                                                                    <  2:5487659/151‑1 (MQ=255)

                 ggAGGCGAGCGGGCCGGGGAAGAAGACGAAGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGAc                                                                                                                                   <  2:1503462/151‑1 (MQ=255)

                    ggCGAGCGGGCCGGGGAAGAAGACGAAGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGACGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTc                                                                                                                                <  2:7281650/151‑1 (MQ=255)

                        cgCGGGCCGGGGAAGAAGACGAAGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGcc                                                                                                                            <  2:7060695/150‑1 (MQ=255)

                           gggCCGGGGAAGAAGACGAAGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGgc                                                                                                                         <  2:4619621/151‑1 (MQ=255)

                               cGGGGAAGAAGACGAAGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGcgccg                                                                                                                     <  1:7939590/151‑1 (MQ=255)

                                ggggAAGAAGACGAAGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGcgccgc                                                                                                                    >  1:11472003/1‑151 (MQ=255)

                                ggggAAGAAGACGAAGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGcgccgc                                                                                                                    >  1:2149610/1‑151 (MQ=255)

                                  ggAAGAAGACGAAGCCCAGGCCGAGGACCTCGAACAGTGCCGAGCACAGCCAGAGCTGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCTTGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCcgcgg                                                                                                                  >  2:1847421/1‑150 (MQ=255)

                                      gaagaCGAAGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGGTGCt                                                                                                              >  2:11644945/1‑151 (MQ=255)

                                       aagaCGAAGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTc                                                                                                             >  2:2651124/1‑151 (MQ=255)

                                        agaCGAAGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCa                                                                                                            <  2:7575087/151‑1 (MQ=255)

                                              aGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTtctc                                                                                                      <  1:5810431/151‑1 (MQ=255)

                                              aGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTtctc                                                                                                      <  1:2066164/151‑1 (MQ=255)

                                                cccAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAg                                                                                                    >  2:6202691/1‑151 (MQ=255)

                                                cccAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAg                                                                                                    <  1:3340217/151‑1 (MQ=255)

                                                cccAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAg                                                                                                    <  1:9227896/151‑1 (MQ=255)

                                                 ccAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAgg                                                                                                   <  2:934938/151‑1 (MQ=255)

                                                  cAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAggg                                                                                                  >  1:10413310/1‑151 (MQ=255)

                                                   aGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGc                                                                                                 >  1:559991/1‑151 (MQ=255)

                                                     gCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTg                                                                                               >  1:6348354/1‑151 (MQ=255)

                                                      ccGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTgg                                                                                              <  1:10004749/151‑1 (MQ=255)

                                                      ccGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGGTGCTCACTTCTCAGGGCTgg                                                                                              >  2:3943669/1‑151 (MQ=255)

                                                          ggACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAgg                                                                                          <  2:6819124/151‑1 (MQ=255)

                                                             ccACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCAc                                                                                       >  2:9036303/1‑151 (MQ=255)

                                                              cACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGAGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCAcc                                                                                      >  1:9487439/1‑151 (MQ=255)

                                                                    cAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGaa                                                                                <  2:9793936/151‑1 (MQ=255)

                                                                    cAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGaa                                                                                >  1:11186755/1‑151 (MQ=255)

                                                                     aGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAg                                                                               >  2:8388442/1‑151 (MQ=255)

                                                                       tGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGAt                                                                             <  1:1279594/151‑1 (MQ=255)

                                                                        gCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATc                                                                            <  2:3786399/151‑1 (MQ=255)

                                                                        gCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATc                                                                            <  2:4687412/151‑1 (MQ=255)

                                                                              cacaGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCg‑gg                                                                     >  1:1819634/1‑151 (MQ=255)

                                                                                  gCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCG‑GGTCGa                                                                 >  1:2901758/1‑151 (MQ=255)

                                                                                   ccAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACGTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCG‑GGTCGAc                                                                >  1:6527116/1‑151 (MQ=255)

                                                                                     agagCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGACGATCGCCG‑GGTCGAccc                                                              >  2:7855214/1‑151 (MQ=255)

                                                                                     agagCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCG‑GGTCGAccc                                                              >  1:4194785/1‑151 (MQ=255)

                                                                                       agCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCG‑GGTCGACCcgc                                                            <  1:4895450/151‑1 (MQ=255)

                                                                                          aGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCG‑GGTCGACCcgcgcg                                                         >  2:6353062/1‑151 (MQ=255)

                                                                                           ggCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCG‑GGTCGACCCGCGcgt                                                        >  2:5350476/1‑151 (MQ=255)

                                                                                           ggCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCG‑GGTCGACCCGCGcgt                                                        >  1:4032659/1‑151 (MQ=255)

                                                                                            gCGACGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCTGGGCTGGAAGGCACCGATGAAGATCGCCG‑GGTCGACCCGCGcgtc                                                       <  2:5880511/151‑1 (MQ=255)

                                                                                              gAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCCGGGCTGGAAGGCACCGATGAAGATCGCCG‑GGTCGACCCGCGcgtcgt                                                     <  2:9482898/151‑1 (MQ=255)

                                                                                              gAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCG‑GGTCGACCCGCGcgtcgt                                                     <  2:11284639/151‑1 (MQ=255)

                                                                                              gAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCG‑GGTCGACCCGCGcgtcgt                                                     >  1:8299125/1‑151 (MQ=255)

                                                                                               aaGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCG‑GGTCGACCCGCGCGTCGtt                                                    >  2:4932321/1‑151 (MQ=255)

                                                                                               aaGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCG‑GGTCGACCCGCGCGTCGtt                                                    <  1:7794742/151‑1 (MQ=255)

                                                                                               aaGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCG‑GGTCGACCCGCGCGTCGtt                                                    >  2:3354566/1‑151 (MQ=255)

                                                                                                aGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCG‑GGTCGACCCGCGCGTCGTTc                                                   >  2:1734162/1‑151 (MQ=255)

                                                                                                aGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCG‑GGTCGACCCGCGCGTCGTTc                                                   >  1:8640742/1‑151 (MQ=255)

                                                                                                 gCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCG‑GGTCGACCCGCGCGTCGTTCa                                                  <  1:9057331/151‑1 (MQ=255)

                                                                                                   gggTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCG‑GGTCGACCCGCGCGTCGTTCAgg                                                >  2:8193788/1‑151 (MQ=255)

                                                                                                      tGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCG‑GGTCGACCCGCGCGTCGTTCAGGCTg                                             >  2:4030212/1‑151 (MQ=255)

                                                                                                         gTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCG‑GGTCGACCCGCGCGTCGTTCAGGCTGACg                                          >  1:10653530/1‑151 (MQ=255)

                                                                                                              gCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCG‑GGTCGACCCGCGCGTCGTTCAGGCTGACGTTCCa                                     <  1:620033/151‑1 (MQ=255)

                                                                                                              gCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCG‑GGTCGACCCGCGCGTCGTTCAGGCTGACGTTCCa                                     <  2:6660826/151‑1 (MQ=255)

                                                                                                              gCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCG‑GGTCGACCCGCGCGTCGTTCAGGCTGACGTTCCa                                     <  1:5464288/151‑1 (MQ=255)

                                                                                                               cGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCG‑GGTCGACCCGCGCGTCGTTCAGGCTGACGTTCCAg                                    >  1:2678223/1‑151 (MQ=255)

                                                                                                               cGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCG‑GGTCGACCCGCGCGTCGTTCAGGCTGACGTTCCAg                                    >  2:5137356/1‑151 (MQ=255)

                                                                                                                ggTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCG‑GGTCGACCCGCGCGTCGTTCAGGCTGACGTTCCAGt                                   >  2:9656949/1‑151 (MQ=255)

                                                                                                                ggTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCG‑GGTCGACCCGCGCGTCGTTCAGGCTGACGTTCCAGt                                   >  2:10962072/1‑151 (MQ=255)

                                                                                                                ggTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCG‑GGTCGACCCGCGCGTCGTTCAGGCTGACGTTCCAGt                                   <  2:8784972/151‑1 (MQ=255)

                                                                                                                ggTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCG‑GGTCGACCCGCGCGTCGTTCAGGCTGACGTTCCAGt                                   >  2:8683058/1‑151 (MQ=255)

                                                                                                                  tgtCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCG‑GGTCGACCCGCGCGTCGTTCAGGCTGACGTTCCAGTGc                                 >  2:4461859/1‑151 (MQ=255)

                                                                                                                    tctcCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCG‑GGTCGACCCGCGCGTCGTTCAGGCTGACGTTCCAGTGCAt                               >  1:9887230/1‑151 (MQ=255)

                                                                                                                        ccTTGGTCATGACAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCG‑GGTCGACCCGCGCGTCGTTCAGGCTGACGTTCCAGTGCATGTGc                           <  1:11122927/151‑1 (MQ=255)

                                                                                                                        ccTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCG‑GGTCGACCCGCGCGTCGTTCAGGCTGACGTTCCAGTGCATGTGc                           >  1:10858480/1‑151 (MQ=255)

                                                                                                                         cTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCG‑GGTCGACCCGCGCGTCGTTCAGGCTGACGTTCCAGTGCATGTGCg                          >  1:7027068/1‑151 (MQ=255)

                                                                                                                         cTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCG‑GGTCGACCCGCGCGTCGTTCAGGCTGACGTTCCAGTGCATGTGCg                          >  1:7562780/1‑151 (MQ=255)

                                                                                                                          ttGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCG‑GGTCGACCCGCGCGTCGTTCAGGCTGACGTTCCAGTGCATGTGCgg                         <  1:2389891/151‑1 (MQ=255)

                                                                                                                           tGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCG‑GGTCGACCCGCGCGTCGTTCAGGCTGACGTTCCAGTGCATGTGCGGt                        >  2:2419871/1‑151 (MQ=255)

                                                                                                                            ggTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCG‑GGTCGACCCGCGCGTCGTTCAGGCTGACGTTCCAGTGCATGTGCGGTc                       >  2:3763728/1‑151 (MQ=255)

                                                                                                                              tCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCG‑GGTCGACCCGCGCGTCGTTCAGGCTGACGTTCCAGTGCATGTGCGGTCCg                     <  2:10538730/151‑1 (MQ=255)

                                                                                                                              tCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCG‑GGTCGACCCGCGCGTCGTTCAGGCTGACGTTCCAGTGCATGTGCGGTCCg                     >  2:4257473/1‑151 (MQ=255)

                                                                                                                              tCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCG‑GGTCGACCCGCGCGTCGTTCAGGCTGACGTTCCAGTGCATGTGCGGTCCg                     >  1:4109069/1‑151 (MQ=255)

                                                                                                                               cATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCG‑GGTCGACCCGCGCGTCGTTCAGGCTGACGTTCCAGTGCATGTGCGGTCCgg                    >  1:1506099/1‑151 (MQ=255)

                                                                                                                               cATCAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCG‑GGTCGACCCGCGCGTCGTTCAGGCTGACGTTCCAGTGCATGTGCGGTCCgg                    >  2:10459170/1‑151 (MQ=255)

                                                                                                                                 tGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCGAGGTCGa                                                                 <  2:2084467/105‑1 (MQ=255)

                                                                                                                                 tGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCG‑GGTCGACCCGCGCGTCGTTCAGGCTGACGTTCCAGTGCATGTGCGGTCCGGTg                  >  1:486561/1‑151 (MQ=255)

                                                                                                                                    aaGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCG‑GGTCGACCCGCGCGTCGTTCAGGCTGACGTTCCAGTGCATGTGCGGTCCGGTGGcc               >  2:4837108/1‑151 (MQ=255)

                                                                                                                                     cggggCGCATCGACGCG‑CCCCCTGGTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCG‑GGTCGACCCGCGCGTCGTTCAGGCTGACGTTCCAGTGCATGTGCGGTCCGGAGGccc              <  1:8782200/150‑1 (MQ=255)

                                                                                                                                           gcATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCG‑GGTCGACCCGCGCGTCGTTCAGGCTGACGTTCCAGTGCATGTGCGGTCCGGTGGCCCTGCCgg        >  2:1338464/1‑151 (MQ=255)

                                                                                                                                            cATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCG‑GGTCGACCCGCGCGTCGTTCAGGCTGACGTTCCAGTGCATGTGCGGTCCGGTGGCCCTGCCGGt       >  1:4468413/1‑151 (MQ=255)

                                                                                                                                            cATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCG‑GGTCGACCCGCGCGTCGTTCAGGCTGACGTTCCAGTGCATGTGCGGTCCGGTGGCCCTGCCGGt       <  2:2823761/151‑1 (MQ=255)

                                                                                                                                             aTCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCG‑GGTCGACCCGCGCGTCGTTCAGGCTGACGTTCCAGTGCATGTGCGGTCCGGTGGCCCTGCCGGTg      >  2:10194514/1‑151 (MQ=255)

                                                                                                                                              tCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCG‑GGTCGACCCGCGCGTCGTTCAGGCTGACGTTCCAGTGCATGTGCGGTCCGGTGGCCCTGCCGGTgg     <  1:735504/151‑1 (MQ=255)

                                                                                                                                              tCGACGCG‑CCCCCTGTACCTTCTGCCCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCG‑GGTCGACCCGCGCGTCGTTCAGGCTGACGTTCCAGTGCATGTGCGGTCCGGTGGCCCTGCCGGTgg     <  2:1201829/151‑1 (MQ=255)

TCGCCGCTGGTGAGCAGGGAGGCGAGCGGGCCGGGGAAGAAGACGAAGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCGCCCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCG‑GGTCGACCCGCGCGTCGTTCAGGCTGACGTTCCAGTGCATGTGCGGTCCGGTGGCCCTGCCGGTGGCGC  >  NC_008463/1280562‑1280858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`