Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,670,543 +A coding (543/1020 nt) PA14_RS16675 → ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,670,5421.A99.8% 1789.2 / ‑3.7 421coding (542/1020 nt)PA14_RS16675ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base A (209/211);  total (210/211)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

Only 100 of 434 total aligned reads displayed.

GACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCGTTTTCCTGG‑AGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTTCCTCCGCGGG  >  NC_008463/3670391‑3670687
                                                                                                                                                        |                                                                                                                                                 
  cGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGG‑aa                                                                                                                                                 >  2:10960756/1‑150 (MQ=255)
   gTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGG‑aag                                                                                                                                                >  1:6559341/1‑149 (MQ=255)
        cGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTgg                                                                                                                                            >  1:9832138/1‑151 (MQ=255)
         gCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGt                                                                                                                                           <  1:1840872/151‑1 (MQ=255)
           ccGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCCAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCg                                                                                                                                         >  2:5814403/1‑151 (MQ=255)
           ccGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCg                                                                                                                                         <  1:7771412/151‑1 (MQ=255)
           ccGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCg                                                                                                                                         >  1:6028555/1‑151 (MQ=255)
             gggTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCggg                                                                                                                                       <  1:8537577/151‑1 (MQ=255)
             gggTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCggg                                                                                                                                       <  2:9229565/151‑1 (MQ=255)
             gggTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCggg                                                                                                                                       <  2:8154074/151‑1 (MQ=255)
              ggTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCgggg                                                                                                                                      <  2:2717810/151‑1 (MQ=255)
              ggTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCgggg                                                                                                                                      >  1:840053/1‑151 (MQ=255)
              ggTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCgggg                                                                                                                                      <  1:872527/151‑1 (MQ=255)
               gTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGc                                                                                                                                     <  1:10317188/151‑1 (MQ=255)
                 gATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTg                                                                                                                                   >  1:7525282/1‑151 (MQ=255)
                  aTTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGc                                                                                                                                  >  2:5468401/1‑151 (MQ=255)
                    ttACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCg                                                                                                                                <  2:7178844/151‑1 (MQ=255)
                      aCGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGt                                                                                                                              <  1:2363571/151‑1 (MQ=255)
                       cGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGTCCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTg                                                                                                                             >  2:11431486/1‑151 (MQ=255)
                       cGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTg                                                                                                                             <  1:11590611/151‑1 (MQ=255)
                       cGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTg                                                                                                                             >  1:7919195/1‑151 (MQ=255)
                        ggCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGc                                                                                                                            >  2:7811801/1‑151 (MQ=255)
                         gCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGct                                                                                                                           <  1:4589595/151‑1 (MQ=255)
                           ttCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGctct                                                                                                                         >  2:3674261/1‑151 (MQ=255)
                            tCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTa                                                                                                                        >  2:10012339/1‑151 (MQ=255)
                             ccGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTAc                                                                                                                       >  1:5503663/1‑151 (MQ=255)
                              cgcgTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTAcc                                                                                                                      >  2:2031866/1‑151 (MQ=255)
                                cgTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACctg                                                                                                                    >  2:8921879/1‑151 (MQ=255)
                                          ctctTCGCCCTGACCCTGACCGTGCTCAGGTCTCTGATCGGCGTCACCGTCGGCGCCCAGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATcc                                                                                                          <  2:8409053/151‑1 (MQ=255)
                                             ttCGCCCTGACCCTGCCCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTgg                                                                                                       <  2:4784672/151‑1 (MQ=255)
                                             ttCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTgg                                                                                                       <  1:10042043/151‑1 (MQ=255)
                                               cGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGcc                                                                                                     >  2:3246600/1‑151 (MQ=255)
                                                     gaccctgaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTc                                                                                               <  1:8140487/151‑1 (MQ=255)
                                                       ccctgaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGt                                                                                             >  1:6649721/1‑151 (MQ=255)
                                                       ccctgaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGt                                                                                             <  1:6308223/151‑1 (MQ=255)
                                                       ccctgaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGt                                                                                             >  2:7939967/1‑151 (MQ=255)
                                                          tgaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCa                                                                                          <  2:3036458/151‑1 (MQ=255)
                                                           gaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAg                                                                                         <  1:4044727/151‑1 (MQ=255)
                                                            accGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGc                                                                                        <  2:9818805/151‑1 (MQ=255)
                                                            accGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGc                                                                                        <  2:2406609/151‑1 (MQ=255)
                                                             ccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGcc                                                                                       >  1:525270/1‑151 (MQ=255)
                                                             ccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGcc                                                                                       <  1:8959300/151‑1 (MQ=255)
                                                                 gCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAAc                                                                                   >  2:9559684/1‑151 (MQ=255)
                                                                 gCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAAc                                                                                   >  1:10814876/1‑151 (MQ=255)
                                                                     aGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCt                                                                               >  2:2238798/1‑151 (MQ=255)
                                                                          tctGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCtggtgg                                                                          <  1:9296200/151‑1 (MQ=255)
                                                                          tctGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCtggtgg                                                                          <  2:1584990/151‑1 (MQ=255)
                                                                          tctGATCGGCGTCACCGTCGGCGCCCAGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCtggtgg                                                                          >  1:8201461/1‑151 (MQ=255)
                                                                            tGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGgct                                                                        <  2:10878083/151‑1 (MQ=255)
                                                                            tGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGgct                                                                        >  1:309526/1‑151 (MQ=255)
                                                                             gATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGgctg                                                                       >  2:515849/1‑151 (MQ=255)
                                                                             gATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGgctg                                                                       <  1:479356/151‑1 (MQ=255)
                                                                                cGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGctgctg                                                                    <  1:3841192/151‑1 (MQ=255)
                                                                                  gCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTggg                                                                  <  2:6319078/151‑1 (MQ=255)
                                                                                  gCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTggg                                                                  >  2:867637/1‑151 (MQ=255)
                                                                                    gTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGca                                                                <  1:2853807/151‑1 (MQ=255)
                                                                                    gTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGca                                                                >  1:717194/1‑151 (MQ=255)
                                                                                     tCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGcat                                                               <  1:4464246/151‑1 (MQ=255)
                                                                                      cACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGcatc                                                              >  1:7251888/1‑151 (MQ=255)
                                                                                       aCCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGcatca                                                             <  2:6214708/151‑1 (MQ=255)
                                                                                       aCCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGcatca                                                             >  1:150955/1‑151 (MQ=255)
                                                                                       aCCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGcatca                                                             >  2:9289709/1‑151 (MQ=255)
                                                                                       aCCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGcatca                                                             >  2:5687359/1‑151 (MQ=255)
                                                                                        ccGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGcatcat                                                            >  2:9414884/1‑151 (MQ=255)
                                                                                          gTCGGCGCCCTGCAAGGCTACTCCGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCATCCGCACTTCTGGTGGCTGCTGGGCATCAtgc                                                          <  2:10358158/151‑1 (MQ=255)
                                                                                          gTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCAtgc                                                          >  2:6877182/1‑151 (MQ=255)
                                                                                          gTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCAtgc                                                          >  1:5796040/1‑151 (MQ=255)
                                                                                            cGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCAtgctg                                                        >  1:9743302/1‑151 (MQ=255)
                                                                                             ggCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCAtgctgc                                                       <  1:1704484/151‑1 (MQ=255)
                                                                                                gcCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGt                                                    <  2:8823393/151‑1 (MQ=255)
                                                                                                  ccTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGttt                                                  >  2:7503123/1‑151 (MQ=255)
                                                                                                   cTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGtttt                                                 >  2:10399839/1‑151 (MQ=255)
                                                                                                      cAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCt                                              <  2:4898769/151‑1 (MQ=255)
                                                                                                      cAAGGCTACAACGGAGGCTGGGTCGACCTGCTCGGCCTGCG‑TTTCCTGGAAGACTGGTCGGGGCTGCCGTTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGGTTTCCt                                              >  1:10578782/1‑151 (MQ=255)
                                                                                                       aaGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTg                                             <  2:2817508/151‑1 (MQ=255)
                                                                                                        aGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTgg                                            <  2:11740196/151‑1 (MQ=255)
                                                                                                         ggCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGa                                           <  1:11345741/151‑1 (MQ=255)
                                                                                                         ggCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGa                                           <  1:1205034/151‑1 (MQ=255)
                                                                                                            tactacGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGa                                        >  2:7365703/1‑151 (MQ=255)
                                                                                                                acGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCt                                    >  2:6169109/1‑151 (MQ=255)
                                                                                                                     ggcTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCtggtgg                               >  1:10713000/1‑151 (MQ=255)
                                                                                                                     ggcTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCtggtgg                               <  2:4336193/151‑1 (MQ=255)
                                                                                                                     ggcTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCtggtgg                               >  1:9172950/1‑151 (MQ=255)
                                                                                                                       cTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGAc                             <  1:8311989/151‑1 (MQ=255)
                                                                                                                       cTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGAc                             <  1:10396669/151‑1 (MQ=255)
                                                                                                                       cTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGAc                             >  2:3555167/1‑151 (MQ=255)
                                                                                                                         gggTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGt                           <  2:10586228/151‑1 (MQ=255)
                                                                                                                          ggTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTg                          >  1:3525320/1‑151 (MQ=255)
                                                                                                                              gACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTcc                      >  2:11165812/1‑151 (MQ=255)
                                                                                                                                 cTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCcgcg                   <  1:10254168/151‑1 (MQ=255)
                                                                                                                                 cTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCcgcg                   <  2:3763391/151‑1 (MQ=255)
                                                                                                                                   gCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGcc                 <  1:3717279/151‑1 (MQ=255)
                                                                                                                                    cTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCg                <  1:4905889/151‑1 (MQ=255)
                                                                                                                                      cGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAg              <  2:6604250/151‑1 (MQ=255)
                                                                                                                                      cGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAg              <  2:4026173/151‑1 (MQ=255)
                                                                                                                                        gCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGtt            <  2:11083272/151‑1 (MQ=255)
                                                                                                                                              cgtttCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTtcctcc       >  1:5344698/3‑151 (MQ=255)
                                                                                                                                              cgtttCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTtcctcc       <  1:11245789/149‑1 (MQ=255)
                                                                                                                                                tttCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTTCCTCcgc     >  2:534593/1‑151 (MQ=255)
                                                                                                                                                   ccTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTTCCTCCGCggg  <  2:1427566/151‑1 (MQ=255)
                                                                                                                                                        |                                                                                                                                                 
GACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCGTTTTCCTGG‑AGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTTCCTCCGCGGG  >  NC_008463/3670391‑3670687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑