Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,688,1360TG25.6% 845.5 / 23.0 295S168A (TCG→GCG) folDbifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD
Reads supporting (aligned to +/- strand):  ref base T (28/170);  new base G (68/0);  total (124/171)
Fisher's exact test for biased strand distribution p-value = 5.03e-41
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 301 total aligned reads displayed.

AAGGACGTGGACGGTTTCCATCCCTACAACATCGGCCGCCTGGCCCAGCGCATGCCCCTGCTGCGCCCCTGCACCCCGAAAGGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTCGGCGCCTC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTTGCTGCTGGGTGGCTGCA‑CCGTCACCGTGACCCACCGCTTCACCCGCGACCTGGCCGACCATGTGTCGCGCGCCGACCTGGTGGTGGTCGCTGCCGGCAAGCCGGGACTGGTCAAGG  >  NC_008463/3687986‑3688286
                                                                                                                                                      |                                                                                                                                                          
aaGGACGTGGACGGTTTCCATCCCTACAACATCGGCCGCCTGGCCCAGCGCATGCCCCTGCTGCGCCCCTGCACCCCGAAAGGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTCGGCGCCt                                                                                                                                                            <  1:6596203/151‑1 (MQ=255)
     cGTGGACGGTTTCCATCCCTACAACATCGGCCGCCTGGCCCAGCGCATGCCCCTGCTGCGCCCCTGCACCCCGAAAGGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTCGGCGCCGC‑G‑TAc                                                                                                                                                     >  2:7125060/1‑151 (MQ=255)
     cGTGGACGGTTTCCATCCCTACAACATCGGCCGCCTGGCCCAGCGCATGCCCCTGCTGCGCCCCTGCACCCCGAAAGGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGGGGGCGCCAC‑gccc                                                                                                                                                      >  1:9104898/1‑148 (MQ=255)
      gTGGACGGTTTCCATCCCTACAACATCGGCCGCCTGGCCCAGCGCATGCCCCTGCTGCGCCCCTGCACCCCGAAAGGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTCGGCGCCTC‑G‑AACa                                                                                                                                                    <  2:4139184/151‑1 (MQ=255)
        ggACGGTTTCCATCCCTACAACATCGGCCGCCTGGCCCAGCGCATGCCCCTGCTGCGCCCCTGCACCCCGAAAGGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTCGGCGCCTC‑G‑AACAtc                                                                                                                                                  <  1:10631873/151‑1 (MQ=255)
        ggACGGTTTCCATCCCTACAACATCGGCCGCCTGGCCCAGCGCATGCCCCTGCTGCGCCCCTGCACCCCGAAAGGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTCGGCGCCTC‑G‑AACAtc                                                                                                                                                  <  2:2510362/151‑1 (MQ=255)
          aCGGTTTCCATCCCTACAACATCGGCCGCCTGGCCCAGCGCATGCCCCTGCTGCGCCCCTGCACCCCGAAAGGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGGGGGGGCCGC‑G‑aaaaacct                                                                                                                                                >  1:2442405/1‑145 (MQ=255)
           cGGTGTCCATCCCTACAACATCGGCCGCCTGGCCCAGCGCATGCCCCTGCTGCGCCCCTGCACCCCGAAAGGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTCGGCGCCTC‑G‑AACAtcgtc                                                                                                                                               <  2:7703432/151‑1 (MQ=255)
              tttCCATCCCTACAACATCGGCCGCCTGGCCCAGCGCATGCCCCTGCTGCGCCCCTGCACCCCGAAAGGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTCGGCGCCGC‑G‑AACAACCTcgg‑c                                                                                                                                           >  2:6766062/1‑151 (MQ=255)
                   aTCCCTACAACATCGGCCGCCTGGCCCAGCGCATGCCCCTGCTGCGCCCCTGCACCCCGAAAGGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGGGGGCGCCGC‑G‑CACAACGTCgg‑ccggcc                                                                                                                                      >  2:5899582/1‑151 (MQ=255)
                    tcacTACAACCTCGGCCGCCTGGCCCAGCGCATGCCCCTGCTGCGCCCCTGCCCCCCGAAAGGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTCGGCGCCTC‑G‑AACATCGTCGG‑CCGGccc                                                                                                                                     <  2:10462783/148‑1 (MQ=255)
                     cccTACAACATCGGCCGCCTGGCCCAGCGCATGCCCCTGCTGCGCCCCTGCACCCCGAAAGGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTCGGCGCCGG‑G‑AACATAGTCGG‑CCGGCCCa                                                                                                                                    >  1:858648/1‑151 (MQ=255)
                       cTACAACATCGGCCGCCTGGCCCAGCGCATGCCCCTGCTGCGCCCCTGCACCCCGCAAGGCATCATGACCCTGCTCGCCAGCACCGGCGCCGAGCTGTACGGCATGGACGCGGTCGTGGGCCGCGACGA‑G‑AACCTAGTAGG‑CCCGGCCATg                                                                                                                                  >  2:4734612/1‑151 (MQ=255)
                        tACAACATCGGCCGCCTGGCCCAGCGCATGCCCCTGCTGCGCCCCTGCACCCCGAAAGGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTCGGCGCCTC‑G‑AACATCGTCGG‑CCGGCCCATgg                                                                                                                                 <  1:8208286/151‑1 (MQ=255)
                          caacaTCGGCCGCCTGGCCCAGCGCATGCCCCTGCTGCGCCCCTGCACCCCGAAAGGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTGGGGGCCCC‑G‑AACAACGGCGG‑GCCGCCCAAGGcg                                                                                                                               >  1:9343774/1‑150 (MQ=255)
                          caacaTCGGCCGCCTGGCCCAGCGCATGCCCCTGCTGCGCCCCTGCACCCCGAAAGGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTCGGCGCCTC‑G‑AACATCGTCGG‑CCGGCCCATGGct                                                                                                                               <  1:5993957/151‑1 (MQ=255)
                           aacaTCGGCCGCCTGGCCCAGCGCATGCCCCTGCTGCGCCCCTGCACCCCGAAAGGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTGGGCGGCGC‑G‑AACAACGTCGG‑CCGGCCCAAGGcgc                                                                                                                              >  2:2660993/1‑149 (MQ=255)
                           aacaTCGGCCGCCTGGCCCAGCGCATGCCCCTGCTGCGCCCCTGCACCCCGAAAGGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTCGGCGCCAC‑G‑AACCTAGTAGG‑CCCGCCCATGGcgc                                                                                                                              >  2:852666/1‑149 (MQ=255)
                           aacaTCGGCCGCCTGGCCCAGCGCATGCCCCTGCTGCGCCCCTGCACCCCGAAAGGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGGCGGCGCCGC‑G‑AACAACGGCGG‑CCGGccccagggtg                                                                                                                              >  2:6114767/1‑144 (MQ=255)
                            acaTCGGCCGCCTGGCCCAGCGCATGCCCCTGCTGCGCCCCTGCACCCCGAAAGGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTCGGCGCCTC‑G‑AACAACCTCGG‑CCGGCCCCAGGctct                                                                                                                             >  2:11191906/1‑151 (MQ=255)
                               tCGGCCGCCTGGCCCAGCGCATGCCCCTGCTGCGCCCCTGCACCCCGAAAGGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTGGGCGCCGC‑G‑AACATCGTCGG‑CCCGCCCATGGGTCTGGa                                                                                                                          >  2:5360490/1‑151 (MQ=255)
                                cGGCCGCCTGGCCCAGCGCATGCCCCTGCTGCGCCCCTGCACCCCGAAAGGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTCGGCGCCGC‑G‑CACAGCGGCGG‑CCCGCCCATGGctcgtgtg                                                                                                                         >  2:6845036/1‑146 (MQ=255)
                                 ggCCGCCTGGCCCAGCGCATGCCCCTGCTGCGCCCCTGCACCCCGAAAGGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTCGGCGCCTC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGt                                                                                                                        <  1:2561684/151‑1 (MQ=255)
                                 ggCCGCCTGGCCCAGCGCATGCCCCTGCTGCGCCCCTGCACCCCGAAAGGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTCGGCGCCTC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGt                                                                                                                        <  1:9422141/151‑1 (MQ=255)
                                   ccgccTGGCCCAGCGCATGCCCCTGCTGCGCCCCTGCACCCCGAAAGGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTCGGCGCCTC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTtg                                                                                                                      <  2:10615360/151‑1 (MQ=255)
                                    cgccTGGCCCAGCGCATGCCCCTGCTGCGCCCCTGCACCCCGAAAGGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTCGGCGCCTC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTtgc                                                                                                                     <  1:7247398/151‑1 (MQ=255)
                                     gccTGGCCCAGCGCATGCCCCTGCTGCGCCCCTGCACCCCGAAAGGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTCGGCGCCTC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTtgct                                                                                                                    <  1:2356575/151‑1 (MQ=255)
                                     gccTGGCCCAGCGCATGCCCCTGCTGCGCCCCTGCACCCCGAAAGGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGGGGGCGCCGC‑G‑AACAACGTAGG‑CCCGCCCAAGGCGCTGGAGTtgct                                                                                                                    >  2:2412164/1‑151 (MQ=255)
                                      ccTGGCCCAGCGCATGCCCCTGCTGCGCCCCTGCACCCCGAAAGGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGGGGGGGCCGC‑C‑GACATCGTCGG‑GCCGCCCCTGGGGCGGGAGTtgggg                                                                                                                   >  2:3952836/1‑148 (MQ=255)
                                        tGGCCCAGCGCATGCCCCTGCTGCGCCCCTGCACCCCGAAAGGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTCGGCGCCTC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTTgctgct                                                                                                                 <  1:1466325/151‑1 (MQ=255)
                                          gCCCAGCGCATGCCCCTGCTGCGCCCCTGCACCCCGAAAGGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTCGGCGCCTC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTTGCTGCTgg                                                                                                               <  2:11478391/151‑1 (MQ=255)
                                          gCCCAGCGCATGCCCCTGCTGCGCCCCTGCACCCCGAAAGGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGGGGGCGGCGCGG‑ACCAACG‑CGG‑CCGGCCCCTGGCGCTGGAGgtggtggggg                                                                                                               >  2:2988967/1‑147 (MQ=255)
                                           cccAGCGCATGCCCCTGCTGCGCCCCTGCACCCCGAAAGGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTCGGCGCCTC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTTGCTGCTggg                                                                                                              <  1:2800805/151‑1 (MQ=255)
                                           cccAGCGCATGCCCCTGCTGCGCCCCTGCACCCCGAAAGGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGGGGGCGCCGC‑C‑CACAAAGTCGG‑CCGGCCCCAGGCGCTGGGGTTGCTGGTggg                                                                                                              >  1:1699013/1‑151 (MQ=255)
                                               gcgcATGCCCCTGCTGCGCCCCTGCACCCCGAAAGGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTCGGCGCCTC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTTGCTGCTGGGTGGc                                                                                                          <  1:7713227/151‑1 (MQ=255)
                                               gcgcATGCCCCTGCTGCGCCCCTGCACCCCGAAAGGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTCGGCGCCTC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTTGCTGCTGGGTGGc                                                                                                          <  1:5691098/151‑1 (MQ=255)
                                                cgcATGCCCCTGCTGCGCCCCTGCACCCCGAAAGGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTCGGCGCCTC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTTGCTGCTGGGTGGCt                                                                                                         <  1:5288364/151‑1 (MQ=255)
                                                   aTGCCCCTGCTGCGCCCCTGCACCCCGAAAGGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTGGGCGCCAC‑G‑AAAAAAGTCGG‑CCGGCCCCAGGCGCTGGAGTTTGTGCTGGGTGGCTGca                                                                                                      >  2:1476204/1‑151 (MQ=255)
                                                       cccTGCTGCGCCCCTGCACCCCGAAAGGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTCGGCGCCTC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTTGCTGCTGGGTGGCTGca‑ccgt                                                                                                 <  1:8419242/151‑1 (MQ=255)
                                                         ctgctgCGCCCCTGCACCCCGAAAGGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTCGGCGCCGC‑GTAACA‑GGTCGG‑GCGGCCCCGGGGGCAGGTGTTTCTGGGGGGGGGCTGga‑ccgaca                                                                                               >  2:332144/1‑151 (MQ=255)
                                                                gcCCCTGCACCCCGAAAGGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGGCGGCGCCAC‑G‑AACAAAGTCGG‑CCGGCCCAAGGCTCAGGAGTTGCTGCGGGGTTGCTGca‑acctcaaccggac                                                                                        >  2:10099537/1‑144 (MQ=255)
                                                                 ccccTGCACCCCGAAAGGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTCGGCGCCTC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTTGCTGCTGGGTGGCTGCA‑CCGTCACCGTGAcc                                                                                       <  2:1632531/151‑1 (MQ=255)
                                                                   ccTGCACCCCGAAAGGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTCGGCGCCTC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTTGCTGCTGGGTGGCTGCA‑CCGTCACCGTGACCCa                                                                                     <  2:11036111/151‑1 (MQ=255)
                                                                   ccTGCACCCCGAAAGGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGGGGGGGCCGG‑G‑AACCACCTCGG‑CCCGCCCCCGGCGCGGGAGGTGGTGGGGGGGGGGTGGT‑CCGGCACCCAGAcccc                                                                                     >  1:4092823/1‑150 (MQ=255)
                                                                   ccTGCACCCCGAAAGGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGGGGGCGCCGC‑G‑AACAACTGCGG‑GCGGCCCATGGCGATGGAGTTGGTGCTGGGTGGCGGCA‑CCGTCAACGTGGCCCa                                                                                     >  2:560319/1‑151 (MQ=255)
                                                                      gCACCCCGAAAGGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTCGGCGCCTC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTTGCTGCTGGGTGGCTGCA‑CCGTCACCGTGACCCACCg                                                                                  <  2:879124/151‑1 (MQ=255)
                                                                       cACCCCGAAAGGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTGGGCGCCGC‑G‑CACAACGGCGG‑CGCGCCCAAGGGTCTGGGGTGTGTGCGGGGTGGGGGGA‑GCGGGACCCGGACCCACCGc                                                                                 >  1:11390193/1‑151 (MQ=255)
                                                                         ccccGAAAGGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTCGGCGCCAC‑G‑CACAACGTCGG‑CCGGCCCCAGGCTCTGGAGTTGCTGCTGGGGGGCTGCA‑CCCGCACCGGGACCCCCCGcgt                                                                               >  2:7640353/1‑149 (MQ=255)
                                                                           ccGAAAGGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTGGGGGCCAC‑G‑AACAAACTCGGCCCGCCCCATGG‑TCTGGGGTTGGTGCGGGGGGGCGGCC‑CCGTCACCCGGTCCCAccccgaga                                                                             >  2:1556379/1‑145 (MQ=255)
                                                                           ccGAAAGGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTCGGCGCCTC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTTGCTGCTGGGTGGCTGCA‑CCGTCACCGTGACCCACCGCTTCa                                                                             <  2:185784/151‑1 (MQ=255)
                                                                              accggCATCCTGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTCGGCGCCTC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTTGCTGCTGGGTGGCTGCA‑CCGTCACCGTGACCCACCGCTTCAccc                                                                          <  2:7271498/148‑1 (MQ=255)
                                                                              aaaGGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGGAGGCGCCGC‑C‑AACAAAGTCGG‑CCGGCCCAATGGTCTGGAGTTGGTGCTGGGTGGGTGCA‑CCGGCACCCTGTACCACCCCCTCAccc                                                                          >  2:4773462/1‑151 (MQ=255)
                                                                                aGGCATCATGACACTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTCGGCGCCTC‑G‑AACATCGTCGG‑CCGGCCCCTGGCTCTGGAGTTGCTGCTGGGTGGCTGCA‑CCGTCACCGTGACCCACCGCTTCACCcgc                                                                        <  2:8153283/151‑1 (MQ=255)
                                                                                 ggCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTCGGCGCCTC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTTGCTGCTGGGTGGCTGCA‑CCGTCACCGTGACCCACCGCTTCACCcgcg                                                                       <  1:4826058/151‑1 (MQ=255)
                                                                                  gCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTCGGCGCCTC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTTGCTGCTGGGTGGCTGCA‑CCGTCACCGTGACCCACCGCTTCACCCGCGa                                                                      <  2:519221/151‑1 (MQ=255)
                                                                                     tcatGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTCGGCGCCTC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTTGCTGCTGGGTGGCTGCA‑CCGTCACCGTGACCCACCGCTTCACCCGCGACCt                                                                   <  2:8261818/151‑1 (MQ=255)
                                                                                      catGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTCGGCGCCTC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTTGCTGCTGGGTGGCTGCA‑CCGTCACCGTGACCCACCGCTTCACCCGCGACCTg                                                                  <  1:9763602/151‑1 (MQ=255)
                                                                                      catGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTCGGCGCCTC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTTGCTGCTGGGTGGCTGCA‑CCGTCACCGTGACCCACCGCTTCACCCGCGACCTg                                                                  <  1:395181/151‑1 (MQ=255)
                                                                                       atGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTGGGCGCCGC‑G‑AAAATCGTCGG‑CCGGCCCCAGTCTCTGGTGTTGGTGCTGGGGGGCTGGA‑CCGTCACCGCGACCCCACCCGTCAACCGCGCCCCgg                                                                 >  2:7414603/1‑151 (MQ=255)
                                                                                       atGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTCGGCGCCTC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTTGCTGCTGGGTGGCTGCA‑CCGTCACCGTGACCCACCGCTTCACCCGCGACCTgg                                                                 <  2:4527085/151‑1 (MQ=255)
                                                                                         gACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTCGGCGCCTC‑G‑CAAAACGTCGG‑CCGGCCCAAGGCGCTGGTGTTGGGGCTGGGTGGGGGCA‑CCGGAACCCGGACCCACCGCGACACCCCCGCCCAGGcc                                                               >  2:2823353/1‑151 (MQ=255)
                                                                                            ccTGCTCGCCAGCACCGGCGCCGACCTGGACGGCATGGACGCGGTCGTGGTCGGCGCCTC‑G‑ACCCTCGTCGG‑CCGGCCCCGGGCTCTGGAGTTGCTGCTGGGTGGCTGCA‑CCGTCACCGTGACCCACCGCTTCACCCGCGACCTGGCCGAc                                                            <  2:9511801/151‑1 (MQ=255)
                                                                                              tGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTCGGCGCCTC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTTGCTGCTGGGTGGCTGCA‑CCGTCACCGTGACCCACCGCTTCACCCGCGACCTGGCCGACCa                                                          <  1:11565227/151‑1 (MQ=255)
                                                                                                  cGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGGCGGCGCCGC‑G‑CACAACGTCGG‑CCGGCCCCTGGCGCGGGAGTTGGGGGGGGGTGGCGGGA‑CCCGCCCCGGGCCCCACCGCTGCACCCCCGCCCTGGGCCAcccggtg                                                      >  2:9299759/1‑146 (MQ=255)
                                                                                                     cAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTCGGCGCCTC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTTGCTGCTGGGTGGCTGCA‑CCGTCACCGTGACCCACCGCTTCACCCGCGACCTGGCCGACCATGTGTcg                                                   <  2:2570523/151‑1 (MQ=255)
                                                                                                      aGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTGGGCGCCAC‑G‑TAAAACGTCGG‑CCGGCCCAAGGCTCTTGAGTTTCATCTTGGTGGCGTGA‑CCGTCACCCTGTCCCACCCCGTAACCCGCGACCAGGGCGGCCAtgtttagc                                                  >  2:6402470/1‑146 (MQ=255)
                                                                                                       gCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTCGGCGCCTC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTTGCTGCTGGGTGGCTGCA‑CCGTCACCGTGACCCACCGCTTCACCCGCGACCTGGCCGACCATGTGTcgcg                                                 <  1:10085998/151‑1 (MQ=255)
                                                                                                       gCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTCGGCGCCTC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTTGCTGCTGGGTGGCTGCA‑CCGTCACCGTGACCCACCGCTTCACCCGCGACCTGGCCGACCATGTGTcgcg                                                 <  1:8124447/151‑1 (MQ=255)
                                                                                                        cACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTCGGCGCCTC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTTGCTGCTGGGTGGCTGCA‑CCGTCACCGTGACCCACCGCTTCACCCGCGACCTGGCCGACCATGTGTcgcgc                                                <  2:9609631/151‑1 (MQ=255)
                                                                                                               gcCGACCTGTACGGCATGGACGCGGTCGTGGTCGGCGCCAC‑G‑AAAAAAGGAGG‑GCGGCCCATGGGGGTGGAGTTGTTGGTGGGTGGGTGCG‑CCCTCACCGTTAACCACCCCGTAAACCCCGACCAGGGCGACCATGTGTCGCGCGCCGAcc                                         >  2:10894816/1‑151 (MQ=255)
                                                                                                                  gACCTGTACGGCATGGACGCGGTCGTGGTCGGGGCCGC‑C‑AAAAACGTCGG‑CCGGCCCAAGGGTCTGGAGTTTGTGCGGGGTGGGTGGACCCGACCCCG‑GACCCACCCCGTCAACCCCCACCAGGCCGACCAGTAGTCGCGCGCCCACCagg                                      >  2:5720056/1‑151 (MQ=255)
                                                                                                                  gACCTGTACGGCATGGACGCGGTCGTGGTCGGCGCCTC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTTGCTGCTGGGTGGCTGCA‑CCGTCACCGTGACCCACCGCTTCACCCGCGACCTGGCCGACCATGTGTCGCGCGCCGACCtgg                                      <  1:3438727/151‑1 (MQ=255)
                                                                                                                  gACCTGTACGGCATGGACGCGGTCGTGGTCGGCGCCTC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTTGCTGCTGGGTGGCTGCA‑CCGTCACCGTGACCCACCGCTTCACCCGCGACCTGGCCGACCATGTGTCGCGCGCCGACCtgg                                      <  1:4688395/151‑1 (MQ=255)
                                                                                                                     cTGTACGGCATGGACGCGGTCGTGGTCGGCGCCTC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTTGCTGCTGGGTGGCTGCA‑CCGTCACCGTGACCCACCGCTTCACCCGCGACCTGGCCGACCATGTGTCGCGCGCCGACCtggtgg                                   <  1:7493836/151‑1 (MQ=255)
                                                                                                                         aCGGCATGGACGCGGTCGTGGTCGGCGCCTC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTTGCTGCTGGGTGGCTGCA‑CCGTCACCGTGACCCACCGCTTCACCCGCGACCTGGCCGACCATGTGTCGCGCGCCGACCTggtggtggt                               <  2:5073180/151‑1 (MQ=255)
                                                                                                                          cGGCATGGACGCGGTCGTGGTCGGCGCCTC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTTGCTGCTGGGTGGCTGCA‑CCGTCACCGTGACCCACCGCTTCACCCGCGACCTGGCCGACCATGTGTCGCGCGCCGACCTGGTGGTGGTc                              <  2:9691597/151‑1 (MQ=255)
                                                                                                                          cGGCATGGACGCGGTCGTGGTCGGCGCCTC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTTGCTGCTGGGTGGCTGCA‑CCGTCACCGTGACCCACCGCTTCACCCGCGACCTGGCCGACCATGTGTCGCGCGCCGACCTGGTGGTGGTc                              <  2:9458753/151‑1 (MQ=255)
                                                                                                                             cATGGACGCGGTCGTGGTCGGCGCCTC‑G‑AACCTCGTCGG‑CCGGCCCATGGCTCTGGAGTTGCTGCTGGGTGGCTGCA‑CCGTCACCGTGACCCACCGCTTCACCCGCGACCTGGCCGACCATGTGTCGCGCGCCGACCTGGTGGTGGTCGCt                           <  2:393029/151‑1 (MQ=255)
                                                                                                                             cATGGACGCGGTCGTGGTCGGCGCCTC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTTGCTGCTGGGTGGCTGCA‑CCGTCACCGTGACCCACCGCTTCACCCGCGACCTGGCCGACCATGTGTCGCGCGCCGACCTGGTGGTGGTCGCt                           <  1:10011539/151‑1 (MQ=255)
                                                                                                                             cATGGACGCGGTCGTGGTCGGCGCCTC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTTGCTGCTGGGTGGCTGCA‑CCGTCACCGTGACCCACCGCTTCACCCGCGACCTGGCCGACCATGTGTCGCGCGCCGACCTGGTGGTGGTCGCt                           <  1:5027057/151‑1 (MQ=255)
                                                                                                                                 gACGCGGTCGTGGTCGGCGCCTC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTTGCTGCTGGGTGGCTGCA‑CCGTCACCGTGACCCACCGCTTCACCCGCGACCTGGCCGACCATGTGTCGCGCGCCGACCTGGTGGTGGTCGCTGCCg                       <  2:7059084/151‑1 (MQ=255)
                                                                                                                                       gTCGTGGTCGGCGCCTC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTTGCTGCTGGGTGGCTGCA‑CCGTCACCGTGACCCACCGCTTCACCCGCGACCTGGCCGACCATGTGTCGCGCGCCGACCTGGTGGTGGTCGCTGCCGGCAAGc                 >  2:11676340/1‑151 (MQ=255)
                                                                                                                                        gcGTGGTCGGCGCCTC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTTGCTGCTGGGTGGCTGCA‑CCGTCACCGTGACCCACCGCTTCACCCGCGACCTGGCCGACCATGTGTCGCGCGCCGTCCGGGTGGTGGTCGCTGCCGGCAAGcc                >  1:6379426/2‑151 (MQ=255)
                                                                                                                                         cGTGGTCGGCGCCTC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTTGCTGCTGGGTGGCTGCA‑CCGTCACCGTGACCCACCGCTTCACCCGCGACCTGGCCGACCATGTGTCGCGCGCCGACCTGGTGGTGGTCGCTGCCGGCAAGCCg               >  2:1905590/1‑151 (MQ=255)
                                                                                                                                         cGTGGTCGGCGCCTC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTTGCTGCTGGGTGGCTGCA‑CCGTCACCGTGACCCACCGCTTCACCCGCGACCTGGCCGACCATGTGTCGCGCGCCGACCTGGTGGTGGTCGCTGCCGGCAAGCCg               <  1:42606/151‑1 (MQ=255)
                                                                                                                                             gTCGGCGCCTC‑G‑AACATCGTCGG‑CCGTCCCATGGCTCTGGAGTTGCTGCTGGGTGGCTGCA‑CCGTCACCGTGACCCACCGCTTCACCCGCGACCTGGCCGACCATGTGTCGCGCGCCGACCGGGAGGTGGTCGCTGCCGGCAAGCCGGGAc           >  1:3168329/1‑151 (MQ=255)
                                                                                                                                             gTCGGCGCCTC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTTGCTGCTGGGTGGCTGCA‑CCGTCACCGTGACCCACCGCTTCACCCGCGACCTGGCCGACCATGTGTCGCGCGCCGACCTGGTGGTGGTCGCTGCCGGCAAGCCGGGAc           >  2:10832188/1‑151 (MQ=255)
                                                                                                                                              tCGGCGCCTC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTTGCTGCTGGGTGGCTGCA‑CCGTCACCGTGACCCACCGCTTCACCCGCGACCTGGCCGACCATGTGTCGCGCGCCGACCTGGTGGTGGTCGCTGCCGGCAAGCCGGGACt          <  1:6268638/151‑1 (MQ=255)
                                                                                                                                              tCGGCGCCTC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTTGCTGCTGGGTGGCTGCA‑CCGTCACCGTGACCCACCGCTTCACCCGCGACCTGGCCGACCATGTGTCGCGCGCCGACCTGGTGGTGGTCGCTGCCGGCAAGCCGGGACt          >  2:8048923/1‑151 (MQ=255)
                                                                                                                                              tCGGCGCCTC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTTGCTGCTGGGTGGCTGCA‑CCGTCACCGTGACCCACCGCTTCACCCGCGACCTGGCCGACCATGTGTCGCGCGCCGACCTGGTGGTGGTCGCTGCCGGCAAGCCGGGACt          <  1:2173111/151‑1 (MQ=255)
                                                                                                                                               cGGCGCCTC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTTGCTGCTGGGTGGCTGCA‑CCGTCACCGTGACCCACCGCTTCACCCGCGACCTGGCCGACCATGTGTCGCGCGCCGACCTGGTGGTGGTCGCTGCCGGCAAGCCGGGACTg         >  2:10193015/1‑151 (MQ=255)
                                                                                                                                                 gcgcCTC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTTGCTGCTGGGTGGCTGCA‑CCGTCACCGTGACCCACCGCTTCACCCGCGACCTGGCCGACCATGTGTCGCGCGCCGACCTGGTGGTGGTCGCTGCCGGCAAGCCGGGACTGGt       <  2:8993893/151‑1 (MQ=255)
                                                                                                                                                  cgcCTC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTTGCTGCTGGGTGGCTGCA‑CCGTCACCGTGACCCACCGCTTCACCCGCGACCTGGCCGACCATGTGTCGCGCGCCGACCTGGTGGTGGTCGCTGCCGGCAAGCCGGGACTGGTc      >  2:6175065/1‑151 (MQ=255)
                                                                                                                                                   gcCTC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTTGCTGCTGGGTGGCTGCA‑CCGTCACCGTGACCCACCGCTTCACCCGCGACCTGGCCGACCATGTGTCGCGCGCCGACCTGGTGGTGGTCGCTGCCGGCAAGCCGGGACTGGTCa     >  2:1789236/1‑151 (MQ=255)
                                                                                                                                                    ccTC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTTGCTGCTGGGTGGCTGCA‑CCGTCACCGTGACCCACCGCTTCACCCGCGACCTGGCCGACCATGTGTCGCGCGCCGACCTGGTGGTGGTCGCTGCCGGCAAGCCGGGACTGGTCaa    <  1:11051715/151‑1 (MQ=255)
                                                                                                                                                      tC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTTGCTGCTGGGTGGCTGCA‑CCGTCACCGTGACCCACCGCTTCACCCGCGACCTGGCCGACCATGTGTCGCGCGCCGACCTGGTGGTGGTCGCTGCCGGCAAGCCGGGACTGGTCAAgg  <  1:7920782/151‑1 (MQ=255)
                                                                                                                                                      tC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTTGCTGCTGGGTGGCTGCA‑CCGTCACCGTGACCCACCGCTTCACCCGCGACCTGGCCGACCATGTGTCGCGCGCCGACCTGGTGGTGGTCGCTGCCGGCAAGCCGGGACTGGTCAAgg  <  1:10820502/151‑1 (MQ=255)
                                                                                                                                                      tC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTTGCTGCTGGGTGGCTGCA‑CCGTCACCGTGACCCACCGCTTCACCCGCGACCTGGCCGACCATGTGTCGCGCGCCGACCTGGTGGTGGTCGCTGCCGGCAAGCCGGGACTGGTCAAgg  >  1:10393835/1‑151 (MQ=255)
                                                                                                                                                      tC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTTGCTGCTGGGTGGCTGCA‑CCGTCACCGTGACCCACCGCTTCACCCGCGACCTGGCCGACCATGTGTCGCGCGCCGACCTGGTGGTGGTCGCTGCCGGCAAGCCGGGACTGGTCAAgg  >  2:6685295/1‑151 (MQ=255)
                                                                                                                                                      tC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTTGCTGCTGGGTGGCTGCA‑CCGTCACCGTGACCCACCGCTTCACCCGCGACCTGGCCGACCATGTGTCGCGCGCCGACCTGGTGGTGGTCGCTGCCGGCAAGCCGGGACTGGTCAAgg  <  1:6474549/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                          
AAGGACGTGGACGGTTTCCATCCCTACAACATCGGCCGCCTGGCCCAGCGCATGCCCCTGCTGCGCCCCTGCACCCCGAAAGGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTCGGCGCCTC‑G‑AACATCGTCGG‑CCGGCCCATGGCTCTGGAGTTGCTGCTGGGTGGCTGCA‑CCGTCACCGTGACCCACCGCTTCACCCGCGACCTGGCCGACCATGTGTCGCGCGCCGACCTGGTGGTGGTCGCTGCCGGCAAGCCGGGACTGGTCAAGG  >  NC_008463/3687986‑3688286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑